[Objectives]This study was conducted to find a convenient and reliable method for detecting the virus titer of porcine circovirus type 2(PCV2).[Methods]The reaction conditions of the immunoperoxidase monolayer assay(I...[Objectives]This study was conducted to find a convenient and reliable method for detecting the virus titer of porcine circovirus type 2(PCV2).[Methods]The reaction conditions of the immunoperoxidase monolayer assay(IPMA)method for detecting the viral titer of PCV2 were optimized,and the results were compared with those obtained by indirect immunofluorescence assay(IFA).[Results]PCV2-infected cells exhibited brownish-red staining in either the nucleus or cytoplasm when detected by IPMA,and the detection results were largely consistent with IFA detection.[Conclusions]Both IPMA and IFA methods can be effectively used for determination of PCV2 viral titer,providing reliable support for assessing viral content during PCV2 vaccine development and validating virus inactivation efficacy.展开更多
基金Supported by General Project of Shandong Provincial Natural Science Foundation(ZR2022MC173)Binzhou Young Science and Technology Rising Star Program(QMX2023004)Binzhou Comprehensive Experimental Station of Shandong Swine Industry Technology System Project(SDAIT-08-13).
文摘[Objectives]This study was conducted to find a convenient and reliable method for detecting the virus titer of porcine circovirus type 2(PCV2).[Methods]The reaction conditions of the immunoperoxidase monolayer assay(IPMA)method for detecting the viral titer of PCV2 were optimized,and the results were compared with those obtained by indirect immunofluorescence assay(IFA).[Results]PCV2-infected cells exhibited brownish-red staining in either the nucleus or cytoplasm when detected by IPMA,and the detection results were largely consistent with IFA detection.[Conclusions]Both IPMA and IFA methods can be effectively used for determination of PCV2 viral titer,providing reliable support for assessing viral content during PCV2 vaccine development and validating virus inactivation efficacy.
