AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： I...AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.展开更多
Purpose:The purpose was to explore the experiences of nurses caring for patients with delirium in ICU in China.Methods:Semi-structured qualitative interviews were conducted with 14 ICU nurses in Beijing,China.Audio re...Purpose:The purpose was to explore the experiences of nurses caring for patients with delirium in ICU in China.Methods:Semi-structured qualitative interviews were conducted with 14 ICU nurses in Beijing,China.Audio recordings of the transcripts were coded and analysed thematically.Results:The emergent themes reflected clearly similar experiences and were titled as follows:Internal and external barriers to care;Care burden:workload,psychological pressure and injury;Dilemmas in decision-making:balancing risks and benefits.Conclusions:The results of this qualitative study have provided a rich description of the perceptions of a sample of nurses caring for patients with dementia in Beijing.Clearly,the nurses suffered from their work experiences in several aspects:they lacked the knowledge and skills required assessing and managing the patients as early as possible;they were physically and psychologically stressed while looking after the patients and faced with dilemmas and compromises in their decision-making.展开更多
突发公共事件期间病人涌入导致重症医学科(intensive care unit, ICU)救治效率降低。为综合评估病人床位分配策略提升ICU救治效率的效果,采用智能体建模-离散事件仿真(agent-based modeling-discrete event simulation, ABM-DES)方法构...突发公共事件期间病人涌入导致重症医学科(intensive care unit, ICU)救治效率降低。为综合评估病人床位分配策略提升ICU救治效率的效果,采用智能体建模-离散事件仿真(agent-based modeling-discrete event simulation, ABM-DES)方法构建ICU救治系统仿真模型。以武汉大学人民医院综合性ICU为例,引入急诊科分区管理策略设计4种差异化床位分配方案,同时从病人、医护人员、物资和空间多维度出发,采用熵权逼近理想解排序法(technique for order preference by similarity to an ideal solution, TOPSIS)法构建ICU救治效率评价体系对多种病人到达模式下的救治效率进行量化评估,筛选最优床位分配方案。结果表明:病人到达模式显著影响最优床位分配方案;分区管理策略的增效作用仅体现在长期大量病人到达场景。研究结果可为ICU应对突发公共事件提供重要参考。展开更多
文摘AIM： To explore the effect of Astraga/us mongholicus polysaccharide （APS） on gene expression and mitogenactivated protein kinase （MAPK） transcriptional activity in intestinal epithelial cells （IEC）. METHODS： IEC were divided into control group, lipopolysaccharide （LPS） group, LPS＋ 50 μg/mL APS group, LPS＋ 100 μg/mL APS group, LPS＋ 200 μg/mL APS group, and LPS＋ 500 μg/mL APS group. Levels of mRNAs in LPS-induced inflammatory factors, tumor necrosis factor （TNF）-α and interleukin （IL）-8, were measured by reverse transcription-polymerase chain reaction. MAPK protein level was measured by Western blotting. RESULTS： The levels of TNF-α and IL-8 mRNAs were significantly higher in IEC with LPS-induced damage than in control cells. APS significantly abrogated the LPS-induced expression of the TNF-α and IL-8 genes. APS did not block the activation of extracellular signal- regulated kinase or c Jun amino-terminal kinase, but inhibited the activation of p38, suggesting that APS inhibits LPS-induced production of TNF-α and IL-8 mRNAs, possibly by suppressing the p38 signaling pathway.CONCLUSION： APS-modulated bacterial productmediated p38 signaling represents an attractive strategy for prevention and treatment of intestinal inflammation.
基金This study was financially supported by the Capital Foundation of Traditional Medicine and Nursing Research(approval number:10ZYH14)Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality(PHR201107114)。
文摘Purpose:The purpose was to explore the experiences of nurses caring for patients with delirium in ICU in China.Methods:Semi-structured qualitative interviews were conducted with 14 ICU nurses in Beijing,China.Audio recordings of the transcripts were coded and analysed thematically.Results:The emergent themes reflected clearly similar experiences and were titled as follows:Internal and external barriers to care;Care burden:workload,psychological pressure and injury;Dilemmas in decision-making:balancing risks and benefits.Conclusions:The results of this qualitative study have provided a rich description of the perceptions of a sample of nurses caring for patients with dementia in Beijing.Clearly,the nurses suffered from their work experiences in several aspects:they lacked the knowledge and skills required assessing and managing the patients as early as possible;they were physically and psychologically stressed while looking after the patients and faced with dilemmas and compromises in their decision-making.
文摘突发公共事件期间病人涌入导致重症医学科(intensive care unit, ICU)救治效率降低。为综合评估病人床位分配策略提升ICU救治效率的效果,采用智能体建模-离散事件仿真(agent-based modeling-discrete event simulation, ABM-DES)方法构建ICU救治系统仿真模型。以武汉大学人民医院综合性ICU为例,引入急诊科分区管理策略设计4种差异化床位分配方案,同时从病人、医护人员、物资和空间多维度出发,采用熵权逼近理想解排序法(technique for order preference by similarity to an ideal solution, TOPSIS)法构建ICU救治效率评价体系对多种病人到达模式下的救治效率进行量化评估,筛选最优床位分配方案。结果表明:病人到达模式显著影响最优床位分配方案;分区管理策略的增效作用仅体现在长期大量病人到达场景。研究结果可为ICU应对突发公共事件提供重要参考。
