Indian Cancer Research Centre (ICRC) bacillus strain (C-44), a candidate vaccine against leprosy is cultured in vitro in Dubos medium enriched with amino acids and human serum. The study was conducted to find out whet...Indian Cancer Research Centre (ICRC) bacillus strain (C-44), a candidate vaccine against leprosy is cultured in vitro in Dubos medium enriched with amino acids and human serum. The study was conducted to find out whether ICRC bacilli obtained from these cultures are coated with anti mycobacterial antibody. Anti-ICRC antibody raised by intradermal inoculation of sonicated ICRC bacilli in rabbits reacted with both human immunoglobulin G (IgG) and antigens of ICRC. Further, ICRC bacilli could also be fluoresced directly with FITC labelled anti human IgG. Positive fluorescence of ICRC could be abolished by digestion of human IgG with trypsin and carbon tetrachloride (CCL4). It is concluded that ICRC bacilli present in the vaccine are coated with human IgG.展开更多
Q：Our readers are certainly fa- miliar with the ＂Red Cross＂ emblem, but maybe not so familiar with the Regional Delegation for East Asia of the International Committee of the Red Cross （ICRC）. Could you please in...Q：Our readers are certainly fa- miliar with the ＂Red Cross＂ emblem, but maybe not so familiar with the Regional Delegation for East Asia of the International Committee of the Red Cross （ICRC）. Could you please introduce the Regional Delegation for East Asia of the ICRC？ Besides routine activities, could you please let us know what has been done by the delegation？ Could you please say something about your Chinese partner？展开更多
镍钴锰三元正极材料主要由Li、Mn、Co、Ni元素组成,并含有微量的杂质元素Na、Mg、Al、Ca、Fe、Cu等,准确高效地测定三元正极材料中这些元素的含量具有重要的工业意义。但传统的测试方法操作繁琐,且难以实现高含量和微量的多元素同步测...镍钴锰三元正极材料主要由Li、Mn、Co、Ni元素组成,并含有微量的杂质元素Na、Mg、Al、Ca、Fe、Cu等,准确高效地测定三元正极材料中这些元素的含量具有重要的工业意义。但传统的测试方法操作繁琐,且难以实现高含量和微量的多元素同步测定。本文利用Plasma Quant MS Elite型ICP-MS,研究了集成碰撞反应池(iCRC)优化、RF射频功率和离子透镜电压等工作参数,并在同一个测试方法中编辑三个测试模式,实现了用HF-HNO3体系-电感耦合等离子体质谱法一次进样、同时测定主要元素和微量杂质元素的含量。本文测试了研究材料中高含量的Li、Mn、Co、Ni和微量的Na、Mg、Al、Ca、Fe、Cu含量,结果显示,各元素标准曲线线性关系良好,相关系数均大于0.9990,检出限为0.01~5.03μg/g,加标回收率为90%~112%,RSD<3%。该方法操作简便、快速、检出限低、准确度高、精确度好。展开更多
文摘Indian Cancer Research Centre (ICRC) bacillus strain (C-44), a candidate vaccine against leprosy is cultured in vitro in Dubos medium enriched with amino acids and human serum. The study was conducted to find out whether ICRC bacilli obtained from these cultures are coated with anti mycobacterial antibody. Anti-ICRC antibody raised by intradermal inoculation of sonicated ICRC bacilli in rabbits reacted with both human immunoglobulin G (IgG) and antigens of ICRC. Further, ICRC bacilli could also be fluoresced directly with FITC labelled anti human IgG. Positive fluorescence of ICRC could be abolished by digestion of human IgG with trypsin and carbon tetrachloride (CCL4). It is concluded that ICRC bacilli present in the vaccine are coated with human IgG.
文摘Q：Our readers are certainly fa- miliar with the ＂Red Cross＂ emblem, but maybe not so familiar with the Regional Delegation for East Asia of the International Committee of the Red Cross （ICRC）. Could you please introduce the Regional Delegation for East Asia of the ICRC？ Besides routine activities, could you please let us know what has been done by the delegation？ Could you please say something about your Chinese partner？
文摘镍钴锰三元正极材料主要由Li、Mn、Co、Ni元素组成,并含有微量的杂质元素Na、Mg、Al、Ca、Fe、Cu等,准确高效地测定三元正极材料中这些元素的含量具有重要的工业意义。但传统的测试方法操作繁琐,且难以实现高含量和微量的多元素同步测定。本文利用Plasma Quant MS Elite型ICP-MS,研究了集成碰撞反应池(iCRC)优化、RF射频功率和离子透镜电压等工作参数,并在同一个测试方法中编辑三个测试模式,实现了用HF-HNO3体系-电感耦合等离子体质谱法一次进样、同时测定主要元素和微量杂质元素的含量。本文测试了研究材料中高含量的Li、Mn、Co、Ni和微量的Na、Mg、Al、Ca、Fe、Cu含量,结果显示,各元素标准曲线线性关系良好,相关系数均大于0.9990,检出限为0.01~5.03μg/g,加标回收率为90%~112%,RSD<3%。该方法操作简便、快速、检出限低、准确度高、精确度好。
