Objective: to investigate the relationship between the mRNA content of ICOS/ICOSL and the pathogenesis of osteoarthritis. Methods: from January 2018 to June 2020, 20 patients with severe OA (severe OA group), 15 patie...Objective: to investigate the relationship between the mRNA content of ICOS/ICOSL and the pathogenesis of osteoarthritis. Methods: from January 2018 to June 2020, 20 patients with severe OA (severe OA group), 15 patients with mild OA (mild OA group), 40 patients with non-Osteoarthritis (case control group) and 60 patients with healthy control group (healthy control group) were selected as experimental subjects, and ICOs and ICOSL signaling pathway were detected. The expression of ICOS and ICOSL protein in the four groups were compared, and the immunohistochemical scores of synovial tissue in the three groups (case control group, mild OA group and severe OA group) were explored. Results: the mRNA content of ICOS gene showed no statistical difference between healthy control group and case control group, mild OA group (P > 0 0.05), but there was statistical difference between severe OA group and healthy control group (P < 0.05). There was no statistical difference between the control group and mild OA patients (P > 0.05), but there was statistical difference between the control group and severe OA group (P < 0.05). There was no significant difference between mild OA group and severe OA group (P > 0.05). The mRNA content of ICOSL gene was statistically different between healthy control group and case control group, mild OA group and severe OA group (P < 0.05). There was statistical difference between case control group and mild OA group and severe OA group (P < 0.05). There was no significant difference between mild OA group and severe OA group (P > 0.05). ICOS protein in synovial tissue: the expression of ICOS protein in synovial tissue in mild group and severe group was significantly higher than that in disease group (P < 0.05), but there was no significant difference in the expression of ICOS protein in severe group and mild group (P > 0.05). ICOSL protein: there was no significant difference in the expression of ICOSL protein among all groups (P > 0.05).展开更多
文摘Objective: to investigate the relationship between the mRNA content of ICOS/ICOSL and the pathogenesis of osteoarthritis. Methods: from January 2018 to June 2020, 20 patients with severe OA (severe OA group), 15 patients with mild OA (mild OA group), 40 patients with non-Osteoarthritis (case control group) and 60 patients with healthy control group (healthy control group) were selected as experimental subjects, and ICOs and ICOSL signaling pathway were detected. The expression of ICOS and ICOSL protein in the four groups were compared, and the immunohistochemical scores of synovial tissue in the three groups (case control group, mild OA group and severe OA group) were explored. Results: the mRNA content of ICOS gene showed no statistical difference between healthy control group and case control group, mild OA group (P > 0 0.05), but there was statistical difference between severe OA group and healthy control group (P < 0.05). There was no statistical difference between the control group and mild OA patients (P > 0.05), but there was statistical difference between the control group and severe OA group (P < 0.05). There was no significant difference between mild OA group and severe OA group (P > 0.05). The mRNA content of ICOSL gene was statistically different between healthy control group and case control group, mild OA group and severe OA group (P < 0.05). There was statistical difference between case control group and mild OA group and severe OA group (P < 0.05). There was no significant difference between mild OA group and severe OA group (P > 0.05). ICOS protein in synovial tissue: the expression of ICOS protein in synovial tissue in mild group and severe group was significantly higher than that in disease group (P < 0.05), but there was no significant difference in the expression of ICOS protein in severe group and mild group (P > 0.05). ICOSL protein: there was no significant difference in the expression of ICOSL protein among all groups (P > 0.05).
