The 32^nd International Conference on Coastal Engineering（ICCE 2010）,the first of its kind ever held in the mainland of China,was convened on June 30 to July 5,2010,in Shanghai,China.650 delegates from over 40 count...The 32^nd International Conference on Coastal Engineering（ICCE 2010）,the first of its kind ever held in the mainland of China,was convened on June 30 to July 5,2010,in Shanghai,China.650 delegates from over 40 countries and 16 institutional exhibitors participated in this great event.展开更多
随着第六代移动通信网络(6th generation mobile network,6G)的不断发展,通感算一体化技术已成为提升未来网络性能和智能化水平的关键技术之一。通感算一体化网络将通信、感知和计算能力深度融合,实现了对信息的全方位获取、传输和处理...随着第六代移动通信网络(6th generation mobile network,6G)的不断发展,通感算一体化技术已成为提升未来网络性能和智能化水平的关键技术之一。通感算一体化网络将通信、感知和计算能力深度融合,实现了对信息的全方位获取、传输和处理,为各类应用场景提供强有力的技术支撑。全面综述了通感算在6G中的应用及其关键技术,探讨了通感算在低空经济、移动通信系统、智能交通系统、工业互联网与智能制造、智慧城市与环境监测等领域中的广泛应用,并深入讨论了通感算融合技术的核心技术,包括通信感知融合(integrated sensing and communication,ISC)技术、通信计算融合(integrated communication and computation,ICC)技术、感知计算融合(integrated sensing and computation,ISAC)技术、通感算融合(integrated sensing,communication,and computation,ISCC)技术及通感算智融合(integrated sensing,communication,computation and intelligence,ISCCI)技术的最新进展。展望了通感算一体化网络在6G时代的发展趋势,重点分析了未来面临的挑战与研究方向。展开更多
型:极狐阿尔法S5,配置TZ220XS645电机。VIN:LNBSC2TK1RZ××××××。行驶里程:18km。故障现象:极狐阿尔法S5可以正常行驶,全景影像不显示。故障诊断:确认故障现象属实,同时该车氛围灯不亮。诊断仪检测电子座...型:极狐阿尔法S5,配置TZ220XS645电机。VIN:LNBSC2TK1RZ××××××。行驶里程:18km。故障现象:极狐阿尔法S5可以正常行驶,全景影像不显示。故障诊断:确认故障现象属实,同时该车氛围灯不亮。诊断仪检测电子座舱控制器ICC存储有故障码U316F88 ICC IBUS1 BUS off,与信息娱乐总线相关的控制器均无法访问。ICC系统故障码如图1所示。该车信息娱乐总线电路如图2所示。展开更多
目的探讨柴胡皂苷D调控CaMKKβ/AMPK信号通路,在功能性消化不良中对胃肠道Cajal间质细胞(Interstitial cells of Cajal,ICCs)细胞自噬的作用及机制。方法分离大鼠原代ICCs细胞,谷氨酸刺激构建ICCs自噬模型,免疫荧光检测Ca2+水平。将原代...目的探讨柴胡皂苷D调控CaMKKβ/AMPK信号通路,在功能性消化不良中对胃肠道Cajal间质细胞(Interstitial cells of Cajal,ICCs)细胞自噬的作用及机制。方法分离大鼠原代ICCs细胞,谷氨酸刺激构建ICCs自噬模型,免疫荧光检测Ca2+水平。将原代ICCs细胞分为对照组、模型组、模型+柴胡皂苷D组、模型+CaMKKβ抑制剂组、模型+柴胡皂苷D+CaMKKβ抑制剂组。透射电镜观察自噬体超微结构,ELISA检测Ghrelin和SP的水平,免疫荧光检测Ca2+和LC-3Ⅱ的表达,Western blot检测LC-3Ⅱ/Ⅰ、CaMKKβ、p-AMPK、Drp1、MFN2、IP3R和RyR的蛋白表达水平。结果谷氨酸诱导的模型组ICCs中LC-3Ⅱ荧光表达增强。柴胡皂苷D干预可降低Ca2+浓度,降低CaMKKβ、AMPK和MFN2水平(P<0.01),增加LC-3Ⅱ/Ⅰ、IP3R、RyR、Drp1、Ghrelin和SP水平(P<0.01)。柴胡皂苷D联合CaMKKβ抑制剂STO-609干预后效果更显著。结论柴胡皂苷D可通过CaMKKβ/AMPK信号通路介导Ca2+外流,影响ICCs细胞过度自噬及胃肠动力相关因子的表达。展开更多
Background:Huoxue Tongjiang decoction(HXTJD)is an effective prescription for treating reflux esophagitis(RE).We investigated the effects of HXTJD on esophageal motility and mucosal inflammation in a rat RE model.Metho...Background:Huoxue Tongjiang decoction(HXTJD)is an effective prescription for treating reflux esophagitis(RE).We investigated the effects of HXTJD on esophageal motility and mucosal inflammation in a rat RE model.Methods:Chemical composition of HXTJD was analyzed by ultrahigh-performance liquid chromatography Q-Orbitrap mass spectrometry(MS).The change rates of mean contraction tension forces,mean amplitudes,and mean frequencies for the lower esophageal sphincter(LES)were recorded using the isolated tissue bath system,mechanical tension transducer,and PowerLab physiological recorder.After weighing the stomach,the phenol red labeling method was used to measure the gastric emptying rate.The LES ultrastructure was observed through transmission electron microscopy.Immunofluorescence and western blotting were used to detect the number of interstitial cells of Cajal(ICC)and the expression levels of c-kit protein,connexin43(Cx43),and stem cell factor(SCF).Flow cytometric analysis and enzyme-linked immunosorbent assay were conducted to detect the percentages of T helper 17(Th17)cells and regulatory T(Treg)cells and the serum concentrations of interleukin 6(IL-6),interleukin 17(IL-17),and interleukin 10(IL-10)in the rats.Results:We identified 28 chemical constituents in HXTJD.Regarding esophageal motility,we revealed that HXTJD increased the mean contraction tension forces,mean amplitudes,and mean frequency change rate of LES and the gastric emptying rate;decreased stomach weight;and improved the LES ultrastructure.Additionally,HXTJD increased the number of ICC-positive cells,and c-kit,Cx43,and SCF expression levels.Regarding esophageal inflammation,HXTJD significantly decreased the percentage of Th17 cells,and IL-6 and IL-17 concentrations,and increased the percentage of Treg cells and IL-10 concentration.Conclusion:HXTJD was found to be efficacious in the rat RE model.It may promote esophageal motility and alleviate the inflammatory response by activating the SCF/c-kit/ICC pathway and regulating the Th17/Treg cell balance.展开更多
文摘The 32^nd International Conference on Coastal Engineering（ICCE 2010）,the first of its kind ever held in the mainland of China,was convened on June 30 to July 5,2010,in Shanghai,China.650 delegates from over 40 countries and 16 institutional exhibitors participated in this great event.
文摘随着第六代移动通信网络(6th generation mobile network,6G)的不断发展,通感算一体化技术已成为提升未来网络性能和智能化水平的关键技术之一。通感算一体化网络将通信、感知和计算能力深度融合,实现了对信息的全方位获取、传输和处理,为各类应用场景提供强有力的技术支撑。全面综述了通感算在6G中的应用及其关键技术,探讨了通感算在低空经济、移动通信系统、智能交通系统、工业互联网与智能制造、智慧城市与环境监测等领域中的广泛应用,并深入讨论了通感算融合技术的核心技术,包括通信感知融合(integrated sensing and communication,ISC)技术、通信计算融合(integrated communication and computation,ICC)技术、感知计算融合(integrated sensing and computation,ISAC)技术、通感算融合(integrated sensing,communication,and computation,ISCC)技术及通感算智融合(integrated sensing,communication,computation and intelligence,ISCCI)技术的最新进展。展望了通感算一体化网络在6G时代的发展趋势,重点分析了未来面临的挑战与研究方向。
文摘型:极狐阿尔法S5,配置TZ220XS645电机。VIN:LNBSC2TK1RZ××××××。行驶里程:18km。故障现象:极狐阿尔法S5可以正常行驶,全景影像不显示。故障诊断:确认故障现象属实,同时该车氛围灯不亮。诊断仪检测电子座舱控制器ICC存储有故障码U316F88 ICC IBUS1 BUS off,与信息娱乐总线相关的控制器均无法访问。ICC系统故障码如图1所示。该车信息娱乐总线电路如图2所示。
文摘目的探讨柴胡皂苷D调控CaMKKβ/AMPK信号通路,在功能性消化不良中对胃肠道Cajal间质细胞(Interstitial cells of Cajal,ICCs)细胞自噬的作用及机制。方法分离大鼠原代ICCs细胞,谷氨酸刺激构建ICCs自噬模型,免疫荧光检测Ca2+水平。将原代ICCs细胞分为对照组、模型组、模型+柴胡皂苷D组、模型+CaMKKβ抑制剂组、模型+柴胡皂苷D+CaMKKβ抑制剂组。透射电镜观察自噬体超微结构,ELISA检测Ghrelin和SP的水平,免疫荧光检测Ca2+和LC-3Ⅱ的表达,Western blot检测LC-3Ⅱ/Ⅰ、CaMKKβ、p-AMPK、Drp1、MFN2、IP3R和RyR的蛋白表达水平。结果谷氨酸诱导的模型组ICCs中LC-3Ⅱ荧光表达增强。柴胡皂苷D干预可降低Ca2+浓度,降低CaMKKβ、AMPK和MFN2水平(P<0.01),增加LC-3Ⅱ/Ⅰ、IP3R、RyR、Drp1、Ghrelin和SP水平(P<0.01)。柴胡皂苷D联合CaMKKβ抑制剂STO-609干预后效果更显著。结论柴胡皂苷D可通过CaMKKβ/AMPK信号通路介导Ca2+外流,影响ICCs细胞过度自噬及胃肠动力相关因子的表达。
基金supported by the National Natural Science Foundation of China(No.81573737 and 82074213)the science foundation of Tianjin Municipal Health Bureau(No.2023169 and 2021045)the Tianjin Municipal Health Commission Science and Technology Project(No.TJWJ2022QN057).
文摘Background:Huoxue Tongjiang decoction(HXTJD)is an effective prescription for treating reflux esophagitis(RE).We investigated the effects of HXTJD on esophageal motility and mucosal inflammation in a rat RE model.Methods:Chemical composition of HXTJD was analyzed by ultrahigh-performance liquid chromatography Q-Orbitrap mass spectrometry(MS).The change rates of mean contraction tension forces,mean amplitudes,and mean frequencies for the lower esophageal sphincter(LES)were recorded using the isolated tissue bath system,mechanical tension transducer,and PowerLab physiological recorder.After weighing the stomach,the phenol red labeling method was used to measure the gastric emptying rate.The LES ultrastructure was observed through transmission electron microscopy.Immunofluorescence and western blotting were used to detect the number of interstitial cells of Cajal(ICC)and the expression levels of c-kit protein,connexin43(Cx43),and stem cell factor(SCF).Flow cytometric analysis and enzyme-linked immunosorbent assay were conducted to detect the percentages of T helper 17(Th17)cells and regulatory T(Treg)cells and the serum concentrations of interleukin 6(IL-6),interleukin 17(IL-17),and interleukin 10(IL-10)in the rats.Results:We identified 28 chemical constituents in HXTJD.Regarding esophageal motility,we revealed that HXTJD increased the mean contraction tension forces,mean amplitudes,and mean frequency change rate of LES and the gastric emptying rate;decreased stomach weight;and improved the LES ultrastructure.Additionally,HXTJD increased the number of ICC-positive cells,and c-kit,Cx43,and SCF expression levels.Regarding esophageal inflammation,HXTJD significantly decreased the percentage of Th17 cells,and IL-6 and IL-17 concentrations,and increased the percentage of Treg cells and IL-10 concentration.Conclusion:HXTJD was found to be efficacious in the rat RE model.It may promote esophageal motility and alleviate the inflammatory response by activating the SCF/c-kit/ICC pathway and regulating the Th17/Treg cell balance.
