Just seeing off the BOth anniversary of the Institute of Chemistry, Chinese Academy of Sciences （ICCAS）, we are here with pleasure to conduct A Special Column for Young Scientists at ICCAS published in Chinese Chemi...Just seeing off the BOth anniversary of the Institute of Chemistry, Chinese Academy of Sciences （ICCAS）, we are here with pleasure to conduct A Special Column for Young Scientists at ICCAS published in Chinese Chemistry Letters （CCL） endeavoring to disseminate positive energy of several enthusiastic chemists and spread their recent research to the global scientific community.展开更多
In a recent effort hunting for cancer biomarkers, researchers from the Institute of Chemistry, Chinese Academy of Science (ICCAS) identified a new DNA aptamer (termed BG2) that only binds to alkaline phosphatase (AP) ...In a recent effort hunting for cancer biomarkers, researchers from the Institute of Chemistry, Chinese Academy of Science (ICCAS) identified a new DNA aptamer (termed BG2) that only binds to alkaline phosphatase (AP) heterodimer rather than its monomers. This newly-identified DNA aptamer, representing as the first molecular probe reported capable of recognizing a protein dimer, could serve as an important tool to uncover the mystery of this particular protein dimer in a biological context. This study was published in Advanced Science on April 9.展开更多
Objective To discuss the approach and technique of the direct microsurgery of intracavemous sinus carotid artery aneurysms (ICCAAns). Methods All the 15 cases of ICCAAns underwent the direct microsurgery via the carot...Objective To discuss the approach and technique of the direct microsurgery of intracavemous sinus carotid artery aneurysms (ICCAAns). Methods All the 15 cases of ICCAAns underwent the direct microsurgery via the carotid artery-cavernous sinus space approach. Results Immediate carotid arteriography after the surgery showed that the aneurysms disappeared and the carotid artery could be showed clearly and normall. In a follow up period ranged frcm 1 months to 9 years,it was showed that,among 5 cases with 333,IV,V a,VI nerve paralysis before the surgery, three completely recovered,one recovered incompletely. All patients regained the capability of undertaking mormal cativities. None experienced rebleeding or neuroparalysis. Conclusion Direct microsurgery via this is approach is an ideal treatment of ICCAAns. 6 refs.展开更多
Transforming growth factor beta(TGF-β)signalling has become an attractive therapeutic target due to its pro-tumorigenic actions on epithelial cells and its immunosuppressive effects in the tumour microenvironment.In ...Transforming growth factor beta(TGF-β)signalling has become an attractive therapeutic target due to its pro-tumorigenic actions on epithelial cells and its immunosuppressive effects in the tumour microenvironment.In intrahepatic cholangiocarcinoma(iCCA),a highly aggressive malignancy of the biliary tract with poor prognosis,the latest clinical trials using TGF-βinhibitors have failed indicating that the specific actions carried out by TGF-βin iCCA are yet not well delineated.Here,we show that TGF-βsignalling is highly active in iCCA and exerts a prominent suppressor effect on tumour cell lines and organoids established from iCCA metastases biopsies,that relies on a functional canonical SMAD2/3/4 signalling.Thus,TGF-βinhibitors promote,instead of inhibiting,tumour cell growth.In this context,a promising strategy is to target intracellular proteins downstream the TGF-βreceptors accounting only for TGF-βpro-tumorigenic actions.NADPH oxidase 4(NOX4),a downstream mediator of the TGF-βsignalling pathway,is strictly expressed in cancer-associated fibroblasts(CAF)of iCCA and acts in concert with NOX1 to regulate CAF functions.Use of a dual NOX4/NOX1 inhibitor impaired CAF actions and reduced tumour growth in vitro and in two different in vivo iCCA experimental models.Collectively,our findings reveal an actionable way to specifically target TGF-βpro-tumorigenic actions in CAF from iCCA without undesirable side effects on tumour cells,suggesting a potentially bright future for dual NOX4/NOX1 inhibitors in the clinics,alone or in combination with other therapies.展开更多
Hepatocellular carcinoma(HCC)and intrahepatic cholangiocarcinoma(iCCA)represent the most prevalent primary malignancies of the liver(1,2).Both exhibit significant heterogeneity and complex tumor microenvironments,whic...Hepatocellular carcinoma(HCC)and intrahepatic cholangiocarcinoma(iCCA)represent the most prevalent primary malignancies of the liver(1,2).Both exhibit significant heterogeneity and complex tumor microenvironments,which contribute to their aggressive nature and poor prognosis.Conventional genomic and transcriptomic methodologies,including bulk sequencing and single-cell RNA sequencing,have identified critical driver mutations and cellular subpopulations.However,these approaches lack the ability to preserve the native spatial architecture of tissues,thereby limiting insights into cellular interactions and functional niches.The emergence of spatial omics technologies addresses this fundamental limitation.By enabling the simultaneous assessment of molecular expression and its precise histological context,these methods provide an unprecedented view of the tumor ecosystem,offering new avenues for understanding hepatobiliary cancer biology and developing targeted therapies.展开更多
Importance:Infantile convulsions and choreoathetosis(ICCA)is a rare neurological disorder.Many affected patients are either misdiagnosed or prescribed multiple antiepileptic drugs.Objective:To explore therapeutic drug...Importance:Infantile convulsions and choreoathetosis(ICCA)is a rare neurological disorder.Many affected patients are either misdiagnosed or prescribed multiple antiepileptic drugs.Objective:To explore therapeutic drug treatments and dosages for ICCA in children.Methods:Detailed clinical features(e.g.,past medical history and family history),genetic features,and treatment outcomes were collected from the records of six patients with ICCA.Results:Mean age at paroxysmal kinesigenic dyskinesia(PKD)onset was 8 years 8 months(range,3-12 years);the clinical presentation was characterized by daily short paroxysmal episodes of dystonia/dyskinesia.All patients had infantile convulsions at less than 1 year of age,and the mean onset age was 5.5 months(range,4-7 months).Two patients had a family history of ICCA,PKD,or benign familial infantile epilepsy.Whole exome sequencing identified the c.649-650insC mutation in PRRT2 in six patients;three mutations were inherited and three were de novo.All patients were prescribed low-dose carbamazepine and showed dramatic improvement with the complete disappearance of dyskinetic episodes after 3 days.They attended follow-up for 5-17 months and were attack-free until the final follow-up.Interpretation:PRRT2 mutations are the primary cause of ICCA.Lowdose carbamazepine monotherapy is effective and well-tolerated in children.展开更多
Intrahepatic cholangiocarcinoma(iCCA)is the second most common type of liver cancer after hepatocellular carcinoma,accounting for 15%of all primary liver neoplasms.In particular,iCCA is the most lethal tumor of these ...Intrahepatic cholangiocarcinoma(iCCA)is the second most common type of liver cancer after hepatocellular carcinoma,accounting for 15%of all primary liver neoplasms.In particular,iCCA is the most lethal tumor of these types,with the greatest increase in incidence in Western countries over the last decade(1,2).Local resection is the only treatment that can achieve cure,but resection rates remain low and survival is still limited to less than 1 year in the presence of locally unresectable or distant metastases(3).Its incidence and mortality have increased in recent years,and the total number of new cases is expected to increase up to 10-fold over the next two to three decades.In recent years,new treatments for iCCA have been developed and overall survival has improved.展开更多
Intrahepatic cholangiocarcinoma(iCCA)is a rare malignancy,constituting approximately 3-5%of hepatic tumors,with an increasing incidence in recent years(1).Early stage iCCA typically presents asymptomatically,often res...Intrahepatic cholangiocarcinoma(iCCA)is a rare malignancy,constituting approximately 3-5%of hepatic tumors,with an increasing incidence in recent years(1).Early stage iCCA typically presents asymptomatically,often resulting in its detection at advanced stages.Surgical resection remains the primary therapeutic approach for iCCA;however,a substantial proportion of patients are precluded from surgery due to disease progression,necessitating a reliance on pharmaceutical interventions(2).展开更多
文摘Just seeing off the BOth anniversary of the Institute of Chemistry, Chinese Academy of Sciences （ICCAS）, we are here with pleasure to conduct A Special Column for Young Scientists at ICCAS published in Chinese Chemistry Letters （CCL） endeavoring to disseminate positive energy of several enthusiastic chemists and spread their recent research to the global scientific community.
文摘In a recent effort hunting for cancer biomarkers, researchers from the Institute of Chemistry, Chinese Academy of Science (ICCAS) identified a new DNA aptamer (termed BG2) that only binds to alkaline phosphatase (AP) heterodimer rather than its monomers. This newly-identified DNA aptamer, representing as the first molecular probe reported capable of recognizing a protein dimer, could serve as an important tool to uncover the mystery of this particular protein dimer in a biological context. This study was published in Advanced Science on April 9.
文摘Objective To discuss the approach and technique of the direct microsurgery of intracavemous sinus carotid artery aneurysms (ICCAAns). Methods All the 15 cases of ICCAAns underwent the direct microsurgery via the carotid artery-cavernous sinus space approach. Results Immediate carotid arteriography after the surgery showed that the aneurysms disappeared and the carotid artery could be showed clearly and normall. In a follow up period ranged frcm 1 months to 9 years,it was showed that,among 5 cases with 333,IV,V a,VI nerve paralysis before the surgery, three completely recovered,one recovered incompletely. All patients regained the capability of undertaking mormal cativities. None experienced rebleeding or neuroparalysis. Conclusion Direct microsurgery via this is approach is an ideal treatment of ICCAAns. 6 refs.
基金supported by Spanish Association for Cancer Research(AECC),Spain,grant#PRYGN211279FABR,to I.F.Agencia Estatal de Investigación(AEI),Ministry of Science and Innovation(MICIN),Spain,cofounded by FEDER funds/Development Fund—a way to build Europe,grant numbers#RTI2018-094079-B-100,#RTC2019-007125-1,to I.F.+12 种基金#PID2019-108651RJ-I00,PID2022-141984OB-I00 and RYC2021-034121-I,to J.V.J.V.funded by AEI,MICIN,through the Retos Investigación grant number PID2019-108651RJ-I00/DOI 10.13039/501100011033 and“Ramon y Cajal”program RYC2021-034121-IThe CIBEREHD,National Biomedical Research Institute on Liver and Gastrointestinal Diseases,is funded by the Instituto de Salud Carlos III,Spainsupported by Inserm,Universitéde Rennes,Ligue Contre le Cancer(R23037NN),INCa(EU TRANSCAN23-002-2023-129,INCa_18688,R21251ND)ITMO Cancer of AVIESAN within the framework of the 2021-2030 Cancer Control Strategy,on funds administered by Inserm(C18007NS,C20013NS,C20014NS)the French Ministry of Health and the French National Cancer Institute(PRT-K20-136)supported by grants from the Spanish Ministry of Science,Innovation and University(PID2019-108008RJ-I00,PID2023-146827OB-I00),FundacióLa MaratóTV3(201931-31)“Ramon y Cajal”program(RYC2020-029098-I)financial support from the“Ligue contre le cancer”(RS24/75-62)from ITMO Cancer of Aviesan on funds administered by Inserm(C21044DS/ASC21044DSA)supported by PID2021-124694OA-I00,MCIN/AEI/10.13039/501100011033FEDER Una manera de hacer Europa,The European Union grant agreement 101077312*Ramon y Cajal program RYC2022-036321-I.
文摘Transforming growth factor beta(TGF-β)signalling has become an attractive therapeutic target due to its pro-tumorigenic actions on epithelial cells and its immunosuppressive effects in the tumour microenvironment.In intrahepatic cholangiocarcinoma(iCCA),a highly aggressive malignancy of the biliary tract with poor prognosis,the latest clinical trials using TGF-βinhibitors have failed indicating that the specific actions carried out by TGF-βin iCCA are yet not well delineated.Here,we show that TGF-βsignalling is highly active in iCCA and exerts a prominent suppressor effect on tumour cell lines and organoids established from iCCA metastases biopsies,that relies on a functional canonical SMAD2/3/4 signalling.Thus,TGF-βinhibitors promote,instead of inhibiting,tumour cell growth.In this context,a promising strategy is to target intracellular proteins downstream the TGF-βreceptors accounting only for TGF-βpro-tumorigenic actions.NADPH oxidase 4(NOX4),a downstream mediator of the TGF-βsignalling pathway,is strictly expressed in cancer-associated fibroblasts(CAF)of iCCA and acts in concert with NOX1 to regulate CAF functions.Use of a dual NOX4/NOX1 inhibitor impaired CAF actions and reduced tumour growth in vitro and in two different in vivo iCCA experimental models.Collectively,our findings reveal an actionable way to specifically target TGF-βpro-tumorigenic actions in CAF from iCCA without undesirable side effects on tumour cells,suggesting a potentially bright future for dual NOX4/NOX1 inhibitors in the clinics,alone or in combination with other therapies.
基金supported by the Beijing-Tianjin-Hebei Basic Research Cooperation Special Project(grant number 22JCZXJC00200)National High Level Hospital Clinical Research Funding(2025-PUMCH-C-030).
文摘Hepatocellular carcinoma(HCC)and intrahepatic cholangiocarcinoma(iCCA)represent the most prevalent primary malignancies of the liver(1,2).Both exhibit significant heterogeneity and complex tumor microenvironments,which contribute to their aggressive nature and poor prognosis.Conventional genomic and transcriptomic methodologies,including bulk sequencing and single-cell RNA sequencing,have identified critical driver mutations and cellular subpopulations.However,these approaches lack the ability to preserve the native spatial architecture of tissues,thereby limiting insights into cellular interactions and functional niches.The emergence of spatial omics technologies addresses this fundamental limitation.By enabling the simultaneous assessment of molecular expression and its precise histological context,these methods provide an unprecedented view of the tumor ecosystem,offering new avenues for understanding hepatobiliary cancer biology and developing targeted therapies.
文摘Importance:Infantile convulsions and choreoathetosis(ICCA)is a rare neurological disorder.Many affected patients are either misdiagnosed or prescribed multiple antiepileptic drugs.Objective:To explore therapeutic drug treatments and dosages for ICCA in children.Methods:Detailed clinical features(e.g.,past medical history and family history),genetic features,and treatment outcomes were collected from the records of six patients with ICCA.Results:Mean age at paroxysmal kinesigenic dyskinesia(PKD)onset was 8 years 8 months(range,3-12 years);the clinical presentation was characterized by daily short paroxysmal episodes of dystonia/dyskinesia.All patients had infantile convulsions at less than 1 year of age,and the mean onset age was 5.5 months(range,4-7 months).Two patients had a family history of ICCA,PKD,or benign familial infantile epilepsy.Whole exome sequencing identified the c.649-650insC mutation in PRRT2 in six patients;three mutations were inherited and three were de novo.All patients were prescribed low-dose carbamazepine and showed dramatic improvement with the complete disappearance of dyskinetic episodes after 3 days.They attended follow-up for 5-17 months and were attack-free until the final follow-up.Interpretation:PRRT2 mutations are the primary cause of ICCA.Lowdose carbamazepine monotherapy is effective and well-tolerated in children.
文摘Intrahepatic cholangiocarcinoma(iCCA)is the second most common type of liver cancer after hepatocellular carcinoma,accounting for 15%of all primary liver neoplasms.In particular,iCCA is the most lethal tumor of these types,with the greatest increase in incidence in Western countries over the last decade(1,2).Local resection is the only treatment that can achieve cure,but resection rates remain low and survival is still limited to less than 1 year in the presence of locally unresectable or distant metastases(3).Its incidence and mortality have increased in recent years,and the total number of new cases is expected to increase up to 10-fold over the next two to three decades.In recent years,new treatments for iCCA have been developed and overall survival has improved.
基金supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science(KAKENHI:21K07184)a grant from Smoking Research Foundation.
文摘Intrahepatic cholangiocarcinoma(iCCA)is a rare malignancy,constituting approximately 3-5%of hepatic tumors,with an increasing incidence in recent years(1).Early stage iCCA typically presents asymptomatically,often resulting in its detection at advanced stages.Surgical resection remains the primary therapeutic approach for iCCA;however,a substantial proportion of patients are precluded from surgery due to disease progression,necessitating a reliance on pharmaceutical interventions(2).
