Lyon IARC多瘤病毒(LIPyV)是2017年于人类皮肤样本中新发现的病毒,为更加了解其致病性和流行病学状况,本试验拟建立一种灵敏度高、可快速检测LIPyV的SYBR Green I实时荧光定量PCR方法,并对该方法的特异性、灵敏性和重复性进行评价。结...Lyon IARC多瘤病毒(LIPyV)是2017年于人类皮肤样本中新发现的病毒,为更加了解其致病性和流行病学状况,本试验拟建立一种灵敏度高、可快速检测LIPyV的SYBR Green I实时荧光定量PCR方法,并对该方法的特异性、灵敏性和重复性进行评价。结果显示:该检测方法的Ct值与标准品模板线性关系良好,相关系数为0.9989;利用该方法检测CBoV、FBoV、AGV2这几种病毒的基因组均没有出现非特异性扩增;敏感性比普通PCR检测方法高100倍;组内和组间重复性试验的变异系数小于1%;通过60份样本检测发现LIPyV阳性率为6.67%,比普通PCR更灵敏可靠。结果表明,本试验建立的SYBR Green I实时荧光定量PCR方法可用于LIPyV的快速检测。展开更多
本文介绍的是WHO所属的国际癌症研究所（IARC）对900种化学物、混合物和接触场所对入致癌性的综合评价结果。每项评价后的方括号中附有化学文摘号，圆括号中附有相应的专著卷号及最近发表的年份。按IARC对评价的结果共分为4组：Ⅰ组为...本文介绍的是WHO所属的国际癌症研究所（IARC）对900种化学物、混合物和接触场所对入致癌性的综合评价结果。每项评价后的方括号中附有化学文摘号，圆括号中附有相应的专著卷号及最近发表的年份。按IARC对评价的结果共分为4组：Ⅰ组为确定的人类致癌物（Carcinogenic to humans），95种；Ⅱ组A为很可能是人类的致癌物（Probably carcinogenic to humans），66种，动物证据充分，有部分流行病学证据；Ⅱ组B为可能是人类的致癌物（Possbly carcinogenic to humans），241种，动物实验证据充分，但流行病学证据不足；Ⅲ组为因目前资料不够，无法对它们的对人类的致癌性进行分类（Not classifiable as to carcinogencity to humans），497种；IV组为很可能不是人类的致癌物，仅1种。该评价资料译自：IARC．IARC Monographs on the Evaluation of Carcinogenic Risks to Humans and their Supplements：A complete list，其网址为http：／／www．iarc．fr，于2004年7月22日更新。展开更多
Introduction:Night shift work is a complex and frequent occupational exposure,and breast cancer stands as the most prevalent cancer in women.The International Agency for Research on Cancer(IARC)has twice classified ni...Introduction:Night shift work is a complex and frequent occupational exposure,and breast cancer stands as the most prevalent cancer in women.The International Agency for Research on Cancer(IARC)has twice classified night shift work as a probable breast carcinogen,with the latest classification in June 2019.Since that time,new epidemiologic data has emerged.Methods:We searched PubMed for original articles based on cohort and case-control studies of“breast cancer and night shift work”published after the IARC evaluation in June 2019.Results:In total six cohorts and four case-control studies were included in our review.Overall,we observed some support for associations between persistent(long duration or high frequency)night shift work and an increase in breast cancer risk,though most studies were relatively small and statistically under-powered.Moreover,the recent studies do not contribute further evidence regarding the interaction with menopausal status,diurnal preference,hormonal subtypes of breast cancer or gene-environment aspects,which were issues that were left from the IARC evaluation.Conclusions:The available new results somewhat consolidate the epidemiological evidence from IARC’s 2019 evaluation,and do not provide further evidence regarding interaction of interest,e.g.menopausal status,etc.Therefore,long term follow-up of prospective cohorts or nested case-control studies,including precise exposure assessment and examinations of relevant interactions such as menopausal status,diurnal preference,hormonal subtypes of breast cancer and gene-environment aspects,are warranted.Meanwhile,protective measures for the night workers should be considered.展开更多
文摘Lyon IARC多瘤病毒(LIPyV)是2017年于人类皮肤样本中新发现的病毒,为更加了解其致病性和流行病学状况,本试验拟建立一种灵敏度高、可快速检测LIPyV的SYBR Green I实时荧光定量PCR方法,并对该方法的特异性、灵敏性和重复性进行评价。结果显示:该检测方法的Ct值与标准品模板线性关系良好,相关系数为0.9989;利用该方法检测CBoV、FBoV、AGV2这几种病毒的基因组均没有出现非特异性扩增;敏感性比普通PCR检测方法高100倍;组内和组间重复性试验的变异系数小于1%;通过60份样本检测发现LIPyV阳性率为6.67%,比普通PCR更灵敏可靠。结果表明,本试验建立的SYBR Green I实时荧光定量PCR方法可用于LIPyV的快速检测。
文摘本文介绍的是WHO所属的国际癌症研究所（IARC）对900种化学物、混合物和接触场所对入致癌性的综合评价结果。每项评价后的方括号中附有化学文摘号，圆括号中附有相应的专著卷号及最近发表的年份。按IARC对评价的结果共分为4组：Ⅰ组为确定的人类致癌物（Carcinogenic to humans），95种；Ⅱ组A为很可能是人类的致癌物（Probably carcinogenic to humans），66种，动物证据充分，有部分流行病学证据；Ⅱ组B为可能是人类的致癌物（Possbly carcinogenic to humans），241种，动物实验证据充分，但流行病学证据不足；Ⅲ组为因目前资料不够，无法对它们的对人类的致癌性进行分类（Not classifiable as to carcinogencity to humans），497种；IV组为很可能不是人类的致癌物，仅1种。该评价资料译自：IARC．IARC Monographs on the Evaluation of Carcinogenic Risks to Humans and their Supplements：A complete list，其网址为http：／／www．iarc．fr，于2004年7月22日更新。
文摘Introduction:Night shift work is a complex and frequent occupational exposure,and breast cancer stands as the most prevalent cancer in women.The International Agency for Research on Cancer(IARC)has twice classified night shift work as a probable breast carcinogen,with the latest classification in June 2019.Since that time,new epidemiologic data has emerged.Methods:We searched PubMed for original articles based on cohort and case-control studies of“breast cancer and night shift work”published after the IARC evaluation in June 2019.Results:In total six cohorts and four case-control studies were included in our review.Overall,we observed some support for associations between persistent(long duration or high frequency)night shift work and an increase in breast cancer risk,though most studies were relatively small and statistically under-powered.Moreover,the recent studies do not contribute further evidence regarding the interaction with menopausal status,diurnal preference,hormonal subtypes of breast cancer or gene-environment aspects,which were issues that were left from the IARC evaluation.Conclusions:The available new results somewhat consolidate the epidemiological evidence from IARC’s 2019 evaluation,and do not provide further evidence regarding interaction of interest,e.g.menopausal status,etc.Therefore,long term follow-up of prospective cohorts or nested case-control studies,including precise exposure assessment and examinations of relevant interactions such as menopausal status,diurnal preference,hormonal subtypes of breast cancer and gene-environment aspects,are warranted.Meanwhile,protective measures for the night workers should be considered.
