The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene ...The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus （SeMNPV）, amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I/Barn H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.展开更多
目的从Neu3/IAP通路探讨竹节参总皂苷(saponins from Panax japonicus,SPJ)对衰老大鼠结肠炎症的改善作用及其可能的机制。方法SD雄性大鼠分为四组,青年组(6月龄)、自然衰老组(24月龄)以及竹节参总皂苷低、高剂量组。SPJ低、高剂量组大...目的从Neu3/IAP通路探讨竹节参总皂苷(saponins from Panax japonicus,SPJ)对衰老大鼠结肠炎症的改善作用及其可能的机制。方法SD雄性大鼠分为四组,青年组(6月龄)、自然衰老组(24月龄)以及竹节参总皂苷低、高剂量组。SPJ低、高剂量组大鼠从18月龄分别给予含10、30mg/kg的SPJ饲料至24月龄。测量结肠组织长度,生化试剂盒检测大鼠结肠组织中MPO水平,免疫组化检测炎症相关蛋白NLRP3、TNF-α的表达,以及结肠组织中紧密连接蛋白ZO-1、claudin-5的表达,酶组织化学染色法检测大鼠十二指肠IAP活性,RT-PCR检测结肠组织中IAP、Neu3mRNA表达。结果与自然衰老组比较,SPJ低、高剂量能显著降低大鼠结肠长度(P<0.05),增强MPO活力(P<0.05或P<0.01),降低炎症相关蛋白NLRP3、TNF-α(P<0.01),增加紧密连接蛋白ZO-1、claudin-5的表达(P<0.01或P<0.05),增强十二指肠IAP活性以及结肠组织中IAP、Neu3mRNA表达(P<0.01或P<0.05)。结论SPJ可能通过Neu3/IAP通路减轻肠道炎症反应。展开更多
本文用CORE-IAF(Coordinated Ocean-ice Reference Experiments–Interannual Forcing)外强迫场分别强迫LICOM3(LASG/IAP Climate System Ocean Model Version 3)和POP2(Parallel Ocean Program version 2)两个海洋模式,并分析了这两个...本文用CORE-IAF(Coordinated Ocean-ice Reference Experiments–Interannual Forcing)外强迫场分别强迫LICOM3(LASG/IAP Climate System Ocean Model Version 3)和POP2(Parallel Ocean Program version 2)两个海洋模式,并分析了这两个模式中太平洋北赤道逆流(NECC)的模拟结果。我们发现LICOM3和POP2模拟的NECC强度均弱于实测,这和Sun et al.(2019)的研究结果一致,也进一步证明了海洋模式中NECC偏弱是CORE-IAF外强迫场造成的,海表风应力及对应的风应力旋度是海洋模式准确模拟NECC的最主要因子。同时,我们也分析了NECC的模拟在动力机制上的差别,这里的动力强迫项包括风应力项、平流项和余项。我们发现模式的外强迫场虽然相同,但是两个模式中各动力强迫项(风应力项、平流项和余项)对NECC模拟的影响并不完全相同。展开更多
文摘The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus （SeMNPV）, amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I/Barn H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.
文摘目的从Neu3/IAP通路探讨竹节参总皂苷(saponins from Panax japonicus,SPJ)对衰老大鼠结肠炎症的改善作用及其可能的机制。方法SD雄性大鼠分为四组,青年组(6月龄)、自然衰老组(24月龄)以及竹节参总皂苷低、高剂量组。SPJ低、高剂量组大鼠从18月龄分别给予含10、30mg/kg的SPJ饲料至24月龄。测量结肠组织长度,生化试剂盒检测大鼠结肠组织中MPO水平,免疫组化检测炎症相关蛋白NLRP3、TNF-α的表达,以及结肠组织中紧密连接蛋白ZO-1、claudin-5的表达,酶组织化学染色法检测大鼠十二指肠IAP活性,RT-PCR检测结肠组织中IAP、Neu3mRNA表达。结果与自然衰老组比较,SPJ低、高剂量能显著降低大鼠结肠长度(P<0.05),增强MPO活力(P<0.05或P<0.01),降低炎症相关蛋白NLRP3、TNF-α(P<0.01),增加紧密连接蛋白ZO-1、claudin-5的表达(P<0.01或P<0.05),增强十二指肠IAP活性以及结肠组织中IAP、Neu3mRNA表达(P<0.01或P<0.05)。结论SPJ可能通过Neu3/IAP通路减轻肠道炎症反应。
文摘本文用CORE-IAF(Coordinated Ocean-ice Reference Experiments–Interannual Forcing)外强迫场分别强迫LICOM3(LASG/IAP Climate System Ocean Model Version 3)和POP2(Parallel Ocean Program version 2)两个海洋模式,并分析了这两个模式中太平洋北赤道逆流(NECC)的模拟结果。我们发现LICOM3和POP2模拟的NECC强度均弱于实测,这和Sun et al.(2019)的研究结果一致,也进一步证明了海洋模式中NECC偏弱是CORE-IAF外强迫场造成的,海表风应力及对应的风应力旋度是海洋模式准确模拟NECC的最主要因子。同时,我们也分析了NECC的模拟在动力机制上的差别,这里的动力强迫项包括风应力项、平流项和余项。我们发现模式的外强迫场虽然相同,但是两个模式中各动力强迫项(风应力项、平流项和余项)对NECC模拟的影响并不完全相同。
