Research progresses on growth characteristics,application effect and optimization technology of I. aquatica floating bed were reviewed;its application problems in eutrophication water were discussed,and its applicatio...Research progresses on growth characteristics,application effect and optimization technology of I. aquatica floating bed were reviewed;its application problems in eutrophication water were discussed,and its application prospect was forecasted.展开更多
本文通过对水蕹菜净化含银废水的室内外静、动态试验研究得出:(1)水蕹菜对离子态银(Ag^+)的去除速率高于对络合态银[Ag(S_2O_3)_2]^(3-)的去除速率即 K_(Ag)+>K[Ag(S_2O_3)_2]^(3-);(2)水蕹菜对银的净化率 R 与污水停留时间 t 呈指...本文通过对水蕹菜净化含银废水的室内外静、动态试验研究得出:(1)水蕹菜对离子态银(Ag^+)的去除速率高于对络合态银[Ag(S_2O_3)_2]^(3-)的去除速率即 K_(Ag)+>K[Ag(S_2O_3)_2]^(3-);(2)水蕹菜对银的净化率 R 与污水停留时间 t 呈指数函数关系(R=Ae^(B,t),A、B为常数,B<0),其表面去除负荷 P_S 则与 t 呈幂函数关系(P_S=At^B,B<0);(3)试验证明水蕹菜是生物净化含银废水的优良品种之一。展开更多
A 45 d pot experiment was conducted to examine the effects of silicon fertilizer or iron fertilizer on the growth of two typical Ipomoea aquatica cultivars（Daye and Liuye） and arsenic（As） accumuation of Daye and L...A 45 d pot experiment was conducted to examine the effects of silicon fertilizer or iron fertilizer on the growth of two typical Ipomoea aquatica cultivars（Daye and Liuye） and arsenic（As） accumuation of Daye and Liuye grown in As-contaminated soils at different As dosage levels. The results showed that the application of these two fertilizers generally enhanced the growth of the plants, which may be partly attributable to the reduction in As toxicity. The addition of these two fertilizers also significantly reduced the uptake of As by the plants though the iron fertilizer was more effective, as compared to the silicon fertilizer. The accumulation of As in shoot portion was weaker for Daye than for Liuye. The research findings obtained from this study have implications for developing cost-effective management strategies to minimize human health impacts from consumption of As-containing I. aquatica.展开更多
A new Coix aquatica Roxb.cyto-type,found in the southwest of Guangxi Zhuang Autonomous Region in China,was identified and analyzed by acetocarmine squashing and fluorescence in situ hybridization(FISH)technique includ...A new Coix aquatica Roxb.cyto-type,found in the southwest of Guangxi Zhuang Autonomous Region in China,was identified and analyzed by acetocarmine squashing and fluorescence in situ hybridization(FISH)technique including genomic in situ hybridization(GISH).This new C.aquatica cyto-type was different from other C.aquatica cyto-types reported in chromosome structure.It was 2n=30 in chromosome number,and ten bivalents plus ten univalents were formed in meiotic prophase and metaphase I.GISH results indicated that its 20 chromosomes were highly homologous to chromosomes of allotetraploid species C.lacryma-jobi L.(2n=20).The 45S and 5S rDNA were respectively located on the two different chromosomes of the C.aquatica.One of chromosomes carrying 45S rDNA showed its chromosome shape and signal location in the C.aquatica as the same as that in C.lacryma-jobi.So did one of chromosomes carrying 5S rDNA.It could be deducted that one of parental candidates for the new C.aquatica cyto-type may be C.lacryma-jobi The other parental candidate may be allooctopolyploid C.aquatica(2n=40),which is similar to the new cyto-type of C.aquatica in aquatic habit and plant morphology.展开更多
[Objective]This paper was to research the water purification mechanism of Ipomoea aquatica and its correlation with algae and rotifer. [Methods]Taking I. aquatica as the test material,Chlorella vulgaris,Scenedesmus ob...[Objective]This paper was to research the water purification mechanism of Ipomoea aquatica and its correlation with algae and rotifer. [Methods]Taking I. aquatica as the test material,Chlorella vulgaris,Scenedesmus obliquus,Microcystis aeroginosa and rotifer Adineta vaga with different densities were added to the hydroponics nutrients solutions of I. aquatica by the hydroponic ecological simulation method. The growth characteristics of I. aquatica,changes of microbial populations and the consumption status of nutrients in the nutritional solution were determined. And the interactions between the plant and the microbial populations were researched. [Results]When I. aquatica seedlings grew to a certain stage,growth of principal root stopped; while the lateral roots emerged greatly; and the nutrition absorption efficiency enhanced. As the inoculation concentration of C. vulgaris increased,root length of I. aquatica increased relatively great due to the competition for nutrients. The competition and allelopathy of M. aeroginosa and S. obliquus restricted the development of root system of I. aquatica. The grazing pressure of Chlorella vulgaris had little effects on M. aeroginosa,but restricted the rapid growth of S. obliquus. [Conclusions]This research provided data support for the application of fish-shrimp-vegetable aquaculture system.展开更多
Objective:To evaluate the anti-bacterial and anti-biofilm activity of ethyl acetate fraction of Rotula aquatica Lour.(EFRA)against clinically isolated uropathogenic Escherichia coli.Methods:In vitro antibacterial and ...Objective:To evaluate the anti-bacterial and anti-biofilm activity of ethyl acetate fraction of Rotula aquatica Lour.(EFRA)against clinically isolated uropathogenic Escherichia coli.Methods:In vitro antibacterial and anti-biofilm studies were employed.The antimicrobial activity of EFRA was assayed by the well diffusion method.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the active fraction were determined by Resazurin method.The time-kill kinetic assay,acridine orange-ethidium bromide staining,propidium iodide uptake assay,and scanning electron microscopic(SEM)analysis were done to evaluate the efficacy of EFRA in killing uropathogenic Escherichia coli.The anti-biofilm activity was determined by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2 H-tetrazolium-bromide(MTT)assay and specific biofilm formation assay.Results:The well diffusion assay of EFRA showed a very clear zone of inhibition against Escherichia coli BRL-17.The MIC and MBC of EFRA were 2.5 mg/m L and 5 mg/m L,respectively.The time-kill kinetic assay,fluorescence microscopic analysis,propidium iodide uptake assay,and SEM analysis displayed the effect of EFRA in killing the bacteria.The MTT assay and specific biofilm formation assay showed that EFRA prevented the formation of biofilms.Conclusions:The results of the present study confirm that EFRA could prevent bacterial growth and inhibit its biofilm formation.展开更多
Using ion beam biotechnology in combination with soil-less plant cultivation on artificial substratum (floating beds), the experiments were conducted with Ipomoea aquatica Forsk. Plants were attached to floating-beds ...Using ion beam biotechnology in combination with soil-less plant cultivation on artificial substratum (floating beds), the experiments were conducted with Ipomoea aquatica Forsk. Plants were attached to floating-beds which were placed on the surface of artificially nutrient-enriched tank water, in order to study the purification and remediation efficiency of ion beam-treated I. aquatica cultivars. The results show that N + ion beams with 25keV energy and dosages of 0, 2.6, 3.9, 5.2, 6.5, 7.8, 9.1×10 13N +(ions)/cm 2 affected I. aquatica dry seeds differently, with the dose of 3.9×10 13N + (ions)/cm 2 improving effectively the performance as expressed by various biological indices. After ion beam application, I. aquatica cultivars grew well in nutrient-enriched water bodies, increasing the growth of leaves and stem, number of leaves, length and area of roots, plant height, and weight more remarkably than observed in the control. The net removing rates of TN, TP were as high as 75% and 82%, respectively. Especially under the dose of 3.9×10 13N +(ions)/cm 2, the net removing rates of TN, TP were highest, for 77% and 85%, respectively. It was proved that ion beam application improves phytoremediation and may be used to purify nutrient rich water bodies.展开更多
Freshwaters are not only used locally in many developing countries but they are often over exploited for domestic purposes, agriculture and disposal of industrial wastes which result in an overload of excess nutrients...Freshwaters are not only used locally in many developing countries but they are often over exploited for domestic purposes, agriculture and disposal of industrial wastes which result in an overload of excess nutrients, harmful chemicals and heavy metals. Plant species together with sediments and water samples collected from eleven aquatic water bodies in the vicinity of industrial units in Kolkata were studied for their potential to uptake Pb, Cd and Cr under field conditions. Cd and Cr concentrations in the sediments were higher than background values considered to be toxic. Alternanthera philoxeroides and Eichhornia crassipes were the two invasive species present, with the former being more widely distributed. Among native plants, Ipomoea aquatica was the most abundant. Metal uptake in the plants differed among species, tissues and sites. Pb and Cd accumulation in root tissues for all plants in most sites suggested an exclusion strategy for metal tolerance. Since I. aquatica is widely consumed in many parts of SE Asia, its metal content should be checked before use since it was found to efficiently translocate both Pb and Cd from roots to shoots. The potential of A. philoxeroides as a metal excluder needs to be explored further since it translocates less to its shoots as compared to E. crassipes and I. aquatica.展开更多
基金Supported by the Program for Zhejiang Leading Team of S&T Innovation,China(2011R50029)Special Fund for the Construction of Modern Agricultural Industry Technology System,China(CARS-46-33)Technology Development Research Special Fund of Hangzhou Research Institute,China(20132231E04)
文摘Research progresses on growth characteristics,application effect and optimization technology of I. aquatica floating bed were reviewed;its application problems in eutrophication water were discussed,and its application prospect was forecasted.
文摘本文通过对水蕹菜净化含银废水的室内外静、动态试验研究得出:(1)水蕹菜对离子态银(Ag^+)的去除速率高于对络合态银[Ag(S_2O_3)_2]^(3-)的去除速率即 K_(Ag)+>K[Ag(S_2O_3)_2]^(3-);(2)水蕹菜对银的净化率 R 与污水停留时间 t 呈指数函数关系(R=Ae^(B,t),A、B为常数,B<0),其表面去除负荷 P_S 则与 t 呈幂函数关系(P_S=At^B,B<0);(3)试验证明水蕹菜是生物净化含银废水的优良品种之一。
基金financially supported by the National High-Tech R&D Program of China(863 Program,2013AA102402)the National Natural Science Foundation of China(41271469)the Science&Technology Planning Project of Guangdong Province,China(2013B020303001,2015A020208012)
文摘A 45 d pot experiment was conducted to examine the effects of silicon fertilizer or iron fertilizer on the growth of two typical Ipomoea aquatica cultivars（Daye and Liuye） and arsenic（As） accumuation of Daye and Liuye grown in As-contaminated soils at different As dosage levels. The results showed that the application of these two fertilizers generally enhanced the growth of the plants, which may be partly attributable to the reduction in As toxicity. The addition of these two fertilizers also significantly reduced the uptake of As by the plants though the iron fertilizer was more effective, as compared to the silicon fertilizer. The accumulation of As in shoot portion was weaker for Daye than for Liuye. The research findings obtained from this study have implications for developing cost-effective management strategies to minimize human health impacts from consumption of As-containing I. aquatica.
文摘A new Coix aquatica Roxb.cyto-type,found in the southwest of Guangxi Zhuang Autonomous Region in China,was identified and analyzed by acetocarmine squashing and fluorescence in situ hybridization(FISH)technique including genomic in situ hybridization(GISH).This new C.aquatica cyto-type was different from other C.aquatica cyto-types reported in chromosome structure.It was 2n=30 in chromosome number,and ten bivalents plus ten univalents were formed in meiotic prophase and metaphase I.GISH results indicated that its 20 chromosomes were highly homologous to chromosomes of allotetraploid species C.lacryma-jobi L.(2n=20).The 45S and 5S rDNA were respectively located on the two different chromosomes of the C.aquatica.One of chromosomes carrying 45S rDNA showed its chromosome shape and signal location in the C.aquatica as the same as that in C.lacryma-jobi.So did one of chromosomes carrying 5S rDNA.It could be deducted that one of parental candidates for the new C.aquatica cyto-type may be C.lacryma-jobi The other parental candidate may be allooctopolyploid C.aquatica(2n=40),which is similar to the new cyto-type of C.aquatica in aquatic habit and plant morphology.
基金Supported by the Fund of Tianjin Key Laboratory of Animal and Plant Resistance in Tianjin Normal University(No.52XS1209)Transformation and Extension Program of Agricultural Science and Technology Achievements in Tianjin City(No.201001040)Doctoral Fund of Tianjin Normal University(No.52LX30)
文摘[Objective]This paper was to research the water purification mechanism of Ipomoea aquatica and its correlation with algae and rotifer. [Methods]Taking I. aquatica as the test material,Chlorella vulgaris,Scenedesmus obliquus,Microcystis aeroginosa and rotifer Adineta vaga with different densities were added to the hydroponics nutrients solutions of I. aquatica by the hydroponic ecological simulation method. The growth characteristics of I. aquatica,changes of microbial populations and the consumption status of nutrients in the nutritional solution were determined. And the interactions between the plant and the microbial populations were researched. [Results]When I. aquatica seedlings grew to a certain stage,growth of principal root stopped; while the lateral roots emerged greatly; and the nutrition absorption efficiency enhanced. As the inoculation concentration of C. vulgaris increased,root length of I. aquatica increased relatively great due to the competition for nutrients. The competition and allelopathy of M. aeroginosa and S. obliquus restricted the development of root system of I. aquatica. The grazing pressure of Chlorella vulgaris had little effects on M. aeroginosa,but restricted the rapid growth of S. obliquus. [Conclusions]This research provided data support for the application of fish-shrimp-vegetable aquaculture system.
基金financial assistance in the form of JRF(to A.Vysakh),from CSIR,Government of India 09/499(0093)/2017-EMR-I
文摘Objective:To evaluate the anti-bacterial and anti-biofilm activity of ethyl acetate fraction of Rotula aquatica Lour.(EFRA)against clinically isolated uropathogenic Escherichia coli.Methods:In vitro antibacterial and anti-biofilm studies were employed.The antimicrobial activity of EFRA was assayed by the well diffusion method.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the active fraction were determined by Resazurin method.The time-kill kinetic assay,acridine orange-ethidium bromide staining,propidium iodide uptake assay,and scanning electron microscopic(SEM)analysis were done to evaluate the efficacy of EFRA in killing uropathogenic Escherichia coli.The anti-biofilm activity was determined by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2 H-tetrazolium-bromide(MTT)assay and specific biofilm formation assay.Results:The well diffusion assay of EFRA showed a very clear zone of inhibition against Escherichia coli BRL-17.The MIC and MBC of EFRA were 2.5 mg/m L and 5 mg/m L,respectively.The time-kill kinetic assay,fluorescence microscopic analysis,propidium iodide uptake assay,and SEM analysis displayed the effect of EFRA in killing the bacteria.The MTT assay and specific biofilm formation assay showed that EFRA prevented the formation of biofilms.Conclusions:The results of the present study confirm that EFRA could prevent bacterial growth and inhibit its biofilm formation.
文摘Using ion beam biotechnology in combination with soil-less plant cultivation on artificial substratum (floating beds), the experiments were conducted with Ipomoea aquatica Forsk. Plants were attached to floating-beds which were placed on the surface of artificially nutrient-enriched tank water, in order to study the purification and remediation efficiency of ion beam-treated I. aquatica cultivars. The results show that N + ion beams with 25keV energy and dosages of 0, 2.6, 3.9, 5.2, 6.5, 7.8, 9.1×10 13N +(ions)/cm 2 affected I. aquatica dry seeds differently, with the dose of 3.9×10 13N + (ions)/cm 2 improving effectively the performance as expressed by various biological indices. After ion beam application, I. aquatica cultivars grew well in nutrient-enriched water bodies, increasing the growth of leaves and stem, number of leaves, length and area of roots, plant height, and weight more remarkably than observed in the control. The net removing rates of TN, TP were as high as 75% and 82%, respectively. Especially under the dose of 3.9×10 13N +(ions)/cm 2, the net removing rates of TN, TP were highest, for 77% and 85%, respectively. It was proved that ion beam application improves phytoremediation and may be used to purify nutrient rich water bodies.
文摘Freshwaters are not only used locally in many developing countries but they are often over exploited for domestic purposes, agriculture and disposal of industrial wastes which result in an overload of excess nutrients, harmful chemicals and heavy metals. Plant species together with sediments and water samples collected from eleven aquatic water bodies in the vicinity of industrial units in Kolkata were studied for their potential to uptake Pb, Cd and Cr under field conditions. Cd and Cr concentrations in the sediments were higher than background values considered to be toxic. Alternanthera philoxeroides and Eichhornia crassipes were the two invasive species present, with the former being more widely distributed. Among native plants, Ipomoea aquatica was the most abundant. Metal uptake in the plants differed among species, tissues and sites. Pb and Cd accumulation in root tissues for all plants in most sites suggested an exclusion strategy for metal tolerance. Since I. aquatica is widely consumed in many parts of SE Asia, its metal content should be checked before use since it was found to efficiently translocate both Pb and Cd from roots to shoots. The potential of A. philoxeroides as a metal excluder needs to be explored further since it translocates less to its shoots as compared to E. crassipes and I. aquatica.
