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Numerical study of unsteady starting characteristics of a hypersonic inlet 被引量:19
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作者 Wang Weixing Guo Rongwei 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2013年第3期563-571,共9页
The impulse and self starting characteristics of a mixed-compression hypersonic inlet designed at Mach number of 6.5 are studied by applying the unsteady computational fluid dynamics (CFD) method. The full Navier–S... The impulse and self starting characteristics of a mixed-compression hypersonic inlet designed at Mach number of 6.5 are studied by applying the unsteady computational fluid dynamics (CFD) method. The full Navier–Stokes equations are solved with the assumption of viscous perfect gas model, and the shear-stress transport (SST) k–x two-equation Reynolds averaged Navier– Stokes (RANS) model is used for turbulence modeling. Results indicate that during impulse starting, the flow field is divided into three zones with different aerodynamic parameters by primary shock and upstream-facing shock. The separation bubble on the shoulder of ramp undergoes a generating, growing, swallowing and disappearing process in sequence. But a separation bubble at the entrance of inlet exists until the freestream velocity is accelerated to the starting Mach number during self starting. The mass flux distribution of flow field is non-uniform because of the interaction between shock and boundary layer, so that the mass flow rate at throat is unsteady during impulse starting. The duration of impulse starting process increases almost linearly with the decrease of freestream Mach number but rises abruptly when the freestream Mach number approaches the starting Mach number. The accelerating performance of booster almost has no influence on the self starting ability of hypersonic inlet. 展开更多
关键词 Hypersonic flow INLET SCRAMJET start UNSTEADY
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Hypersonic starting flow at high angle of attack 被引量:4
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作者 Bai Chenyuan Wu Ziniu 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2016年第2期297-304,共8页
Compressible starting flow at small angle of attack (AoA) involves small amplitude waves and time-dependent lift coefficient and has been extensively studied before. In this paper we consider hypersonic starting flo... Compressible starting flow at small angle of attack (AoA) involves small amplitude waves and time-dependent lift coefficient and has been extensively studied before. In this paper we consider hypersonic starting flow of a two-dimensional flat wing or airfoil at large angle of attack involving strong shock waves. The flow field in some typical regions near the wing is solved analytically. Simple expressions of time-dependent lift evolutions at the initial and final stages are given. Numerical simulations by compuational fluid dynamics are used to verify and complement the theoretical results. It is shown that below the wing there is a straight oblique shock (OSW) wave, a curved shock wave (CSW) and an unsteady horizontal shock wave (USW), and the latter moves perpendicularlly to the wing. The length of these three parts of waves changes with time. The pres- sure above OSW is larger than that above USW, while across CSW there is a significant drop of the pressure, making the force nearly constant during the initial period of time. When, however, the Mach number is very large, the force coefficient tends to a time-independent constant, proportional to the square of the sine of the angle of attack. 展开更多
关键词 HYPERSONIC Normal force Shock waves starting flow Unsteady flow
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SiC_(f)/SiC复合材料纳秒激光烧蚀机理研究
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作者 邸腾达 杨煜珩 +2 位作者 赵卿钰 吴东江 马广义 《表面技术》 北大核心 2026年第2期124-133,共10页
目的建立工艺参数与纳秒激光烧蚀SiC_(f)/SiC复合材料烧蚀槽尺寸及烧蚀槽形貌的映射关系,揭示不同工艺参数条件下的烧蚀机理,明确烧蚀过程的成分变化规律,为SiC_(f)/SiC复合材料的孔加工和槽加工提供理论指导和工艺基础。方法通过不同... 目的建立工艺参数与纳秒激光烧蚀SiC_(f)/SiC复合材料烧蚀槽尺寸及烧蚀槽形貌的映射关系,揭示不同工艺参数条件下的烧蚀机理,明确烧蚀过程的成分变化规律,为SiC_(f)/SiC复合材料的孔加工和槽加工提供理论指导和工艺基础。方法通过不同激光能量密度和扫描速度的纳秒激光对SiC_(f)/SiC复合材料进行烧蚀,采用扫描电子显微镜、激光共聚焦显微镜以及显微拉曼光谱仪对烧蚀槽形貌尺寸及物相组成进行分析,揭示纳秒激光烧蚀SiC_(f)/SiC复合材料的烧蚀机理。结果在选定工艺参数范围内,激光能量密度主要影响烧蚀宽度,扫描速度主要影响烧蚀深度,激光能量密度由1.78 J/cm^(2)提高到8.89 J/cm^(2),烧蚀宽度提高了10.06μm。扫描速度由800 mm/s降低到50 mm/s,烧蚀深度提高了76.12μm。结论纳秒激光烧蚀SiC_(f)/SiC复合材料的主要烧蚀产物是SiO_(2)。烧蚀槽内部由于氧分压较小,温度较高,SiC被动氧化会转变为活性氧化,生成SiO和CO等物质。此外,烧蚀过程中SiC会发生分解和蒸发,对材料中SiC的含量和结晶度造成影响。当材料吸收的单位时间激光能量输入较低时,SiC蒸发分解产生的气体反冲压力将熔融液滴抛离,在光斑附近形成半圆形溅射状。当材料吸收单位时间激光能量输入较大时,大部分熔融液滴无法离开烧蚀槽,在烧蚀槽内形成较厚的重铸层和块状堆积物。 展开更多
关键词 纳秒激光 SiC_(f)/SiC复合材料 形貌特征 去除机理
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Reconstruction of the starting time series of rainy season in Yunnan and the evolvement of summer monsoon during 1711-1982 被引量:2
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作者 YANG Yuda MAN Zhimin ZHENG Jingyun 《Journal of Geographical Sciences》 SCIE CSCD 2007年第2期212-220,共9页
According to the textual research into the historical documents dominated by archives yearly, as well as the verification with several other kinds of data, the later or earlier starting time of the rainy seasons in Yu... According to the textual research into the historical documents dominated by archives yearly, as well as the verification with several other kinds of data, the later or earlier starting time of the rainy seasons in Yunnan during 1711-1982 has been reconstructed. The analysis indicates that there are obvious fluctuations in the starting date of the rainy seasons in Yunnan in a year or years, and long fluctuation on the decadal scale. The rainy season comes earlier in the early 18th century, later in the 19th century and earlier again in the 20th century. This reflects to a certain degree the gradual change of the summer monsoon in Yunnan. There exists an obvious quasi-3 years cycle, which is related to EI-Nino's quasi-3 years cycle, and a 11.3-year cycle which is notably related to the 11-year cycle of the solar activity of starting date of the rainy seasons in Yunnan. Meanwhile, the dissertation finds that the EI-Nino is very important to the starting date of the rainy seasons in Yunnan. The starting date of the rainy seasons in Yunnan often comes later or normally in the year of EI-Nino. However, there is an obvious imperfect period in such influence, which in turn may mean that there is a certain fluctuation in the effect of ENSO on Asian summer monsoon. 展开更多
关键词 YUNNAN Qing Dynasty starting date of rainy season summer monsoon ENSO
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Review of theoretical achievements for starting flow problem for all Mach numbers 被引量:1
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作者 Chenyuan BAI Juan LI +2 位作者 Sheng LI Sicheng LI Ziniu WU 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2019年第1期78-91,共14页
The typical behavior of unsteady flow and force evolution in a number of applications,such as aero-elastics, gust-wing interaction, flapping flight and flight maneuvering, can be understood using the starting flow mod... The typical behavior of unsteady flow and force evolution in a number of applications,such as aero-elastics, gust-wing interaction, flapping flight and flight maneuvering, can be understood using the starting flow model. Starting flow model is obtained either by setting rapidly an angle of attack for a wing moving at constant speed, or by accelerating a wing to a constant speed while gaining an angle of attack. In the limiting case of impulsively starting flow, the wing is assumed to gain suddenly an angle of attack in an initially uniform flow. Theories have been developed for impulsively starting flow at small angle of attack long before and at large angle of attack only recently, especially for incompressible and supersonic flow. This paper intends to provide a state-of-art overview of the typical flow phenomena, force evolution characteristics and developed theories for impulsively starting flow at any angle of attack and for both lower speed flow(vortex dominated) and high speed flow(compressible wave dominated). This review also provides some new topics that deserve further studies. 展开更多
关键词 Shock-shock interaction Shock WAVES starting fLOW UNSTEADY fLOW VORTEX
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Warm Deformation Behavior of Steels Containing Carbon of 0.45% to 1.26% With Martensite Starting Structure 被引量:1
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作者 LI Qun WANG Tian-sheng LI Hong-biao GAO Yu-wei LINing JING Tian-fu 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2010年第5期34-37,共4页
Warm deformation was investigated for steels containing carbon of 0. 45%,0. 79%, and 1. 26% respeetively with martensite starting structure, using Gleeble 3500 thermomechanical simulator at the temperature ranging fro... Warm deformation was investigated for steels containing carbon of 0. 45%,0. 79%, and 1. 26% respeetively with martensite starting structure, using Gleeble 3500 thermomechanical simulator at the temperature ranging from 873 K to 973 K and the strain rate ranging from 0.1 s^-1 to 0. 001 s^-1. The effect of carbon content on the deformation activation energy, Z value and the deformation resistance was analyzed, The results showed that the deformation resistance decreased with increasing carbon content. The peak stress of the steel containing earbon of 1.26% was decreased by 16.2% compared to the steel containing carbon of 0.45% under the same condition. This may be attributed to the weakening effect of solid solution strengthening which outweighs the precipitation strengthening of cementite. 展开更多
关键词 carbon steel martensite starting structure warm deformation deformation resistance
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Starting flow in a rotating parallel plate channel 被引量:1
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作者 Chang Yi Wang 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2012年第5期1271-1276,共6页
The starting flow due to a suddenly applied pressure gradient in a parallel plate channel which is rotating as a system is studied. Exact analytic series solutions to the unsteady Navier-Stokes equations are found by ... The starting flow due to a suddenly applied pressure gradient in a parallel plate channel which is rotating as a system is studied. Exact analytic series solutions to the unsteady Navier-Stokes equations are found by both the Laplace transform method and the separation of parameters method, the latter is shown to be superior. Rotation not only induces a secondary transverse flow but also alters the character of the transient flow rate and velocity profiles. Back flow and inertial oscillations occur, especially at higher rota- tion rates. 展开更多
关键词 Rotating system flow - starting
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Variations in the starting date of the pre-summer rainy season in South China, 1736-2010 被引量:1
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作者 DING Lingling GE Quansheng +1 位作者 ZHENG Jingyun HAO Zhixin 《Journal of Geographical Sciences》 SCIE CSCD 2014年第5期845-857,共13页
The starting dates of the pre-summer rainy season during historical times (1736- 1911) in Fuzhou and Guangzhou of South China, were determined and reconstructed on the basis of historical documents in the Yu-Xue-Fen... The starting dates of the pre-summer rainy season during historical times (1736- 1911) in Fuzhou and Guangzhou of South China, were determined and reconstructed on the basis of historical documents in the Yu-Xue-Fen-Cun archive, together with observed features of precipitation during the pre-summer rainy season. In addition, starting dates of the pre-summer rainy season from 1953 in Fuzhou and from 1952 in Guangzhou were reconstructed for the instrumental period. These data allowed for analyses of inter-annual and inter-decadal changes in the starting dates of the pre-summer rainy season in South China over the past 300 years. Results show that the mean starting date of the pre-summer rainy season in South China was the first pentad of May; in addition, periodicities in the starting dates of 2-3 years, 10 years, and 40 years were detected during the period 1736-1911, and of 2-3 years, 10 years, and 22 years during the instrumental period. From 1736 to 1911, the earliest starting dates at Fuzhou and Guangzhou both occurred at the fourth pentad of April, while the latest starting dates were at the sixth pentad of May in Fuzhou and the first pentad of June in Guangzhou. During the instrumental period, the earliest and latest starting dates were at the fourth pentad of April and the first pentad of June, respectively, in both Fuzhou during 1953-2010 and Guangzhou during 1952-2010. The maximum difference between neighboring decades during 1736-1911 was 2.2 and 1.6 pentads in Fuzhou and Guangzhou, respectively, and during the instrumental period it was 2.5 and 2.4 pentads in Fuzhou and Guangzhou, respectively. 展开更多
关键词 South China starting date of the pre-summer rainy season reconstruction variation Yu-Xue-fen-Cun
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Simulation study on starting of dual clutch transmission for tracked vehicles 被引量:1
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作者 张金乐 马彪 +1 位作者 张英锋 郑长松 《Journal of Beijing Institute of Technology》 EI CAS 2011年第1期77-83,共7页
In order to study the application of dual clutch transmission (DCT) on tracked vehicles, a simulation moclel and two control methods were performed for starting analysis. Based on a certain transmission of an existi... In order to study the application of dual clutch transmission (DCT) on tracked vehicles, a simulation moclel and two control methods were performed for starting analysis. Based on a certain transmission of an existing tracked vehicle, a DCT structure was proposed. Matlab/Simulink was a dopted as a platform to develop the simulation model. The engine speed was controlled to follow the target speed as a launch strategy. Two control methods, a proportional integral derivative ( PID ) control method and a fuzzy control method, were proposed to control the engine throttle and oil pressure in order to track the target engine speed. Simulation results show that either the PID control or fuzzy control method can improve the starting performance compared with no loop control meth od. Fuzzy control method can lead a better starting quality compared with PID control method. 展开更多
关键词 starting control dual clutch transmission proportional integral derivative (PID) fuzz y tracked vehicle
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Subsonic impulsively starting flow at a high angle of attack with shock wave and vortex interaction 被引量:1
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作者 Chenyuan BAI Juan LI Ziniu WU 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2018年第9期1822-1828,共7页
Impulsively starting flow, by a sudden attainment of a large angle of attack, has been well studied for incompressible and supersonic flows, but less studied for subsonic flow. Recently,a preliminary numerical study f... Impulsively starting flow, by a sudden attainment of a large angle of attack, has been well studied for incompressible and supersonic flows, but less studied for subsonic flow. Recently,a preliminary numerical study for subsonic starting flow at a high angle of attack displays an advance of stall around a Mach number of 0.5, when compared to other Mach numbers. To see what happens in this special case, we conduct here in this paper a further study for this case, to display and analyze the full flow structures. We find that for a Mach number around 0.5, a local supersonic flow region repeatedly splits and merges, and a pair of left-going and right-going unsteady shock waves are embedded inside the leading edge vortex once it is sufficiently grown up and detached from the leading edge. The flow evolution during the formation of shock waves is displayed in detail. The reason for the formation of these shock waves is explained here using the Laval nozzle flow theory. The existence of this shock pair inside the vortex, for a Mach number only close to 0.5, may help the growing of the trailing edge vortex responsible for the advance of stall observed previously. 展开更多
关键词 Advance of stall Compressible vortex High angle of attack Subsonic starting flow Unsteady shock waves
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Starting Characteristics of Motor in Inverter-Motor Drive System 被引量:1
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作者 Xi-jun Yang Xiang-jun Zhang You-min Gong 《Advances in Manufacturing》 2000年第2期140-144,共5页
When the induction motor is fed from the PWM VSI inverter, with and without torque enhancing function, the variations of the starting torque and the starting current versus the starting frequency are analyzed, base... When the induction motor is fed from the PWM VSI inverter, with and without torque enhancing function, the variations of the starting torque and the starting current versus the starting frequency are analyzed, based on the steady state equivalent circuit of the induction motor.The corresponding expressions are derived, and the corresponding curves are also drawn, from which the initial starting frequency range can be determined properly and easily. The initial starting frequency range is the common range, in which the starting torque should be high enough and the starting current low enough.Some other useful formulas are also derived.These results are significant for electrical drive designers to make the starting schemes. The effects of the increase of the stator and the rotor resistances on the starting torque and the initial starting frequency range are also discussed, which is caused by the skin effects of the high order harmonics present in the PWM inverter output voltage. 展开更多
关键词 inverter motor drive system starting characteristics skin effect
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Experimental study of the critical sand starting velocity of gas-watersand flow in an inclined pipe 被引量:3
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作者 Rui-Yao Zhang Jun Li +3 位作者 Hong-Wei Yang Geng Zhang Xi-Ning Hao Peng-Lin Liu 《Petroleum Science》 SCIE CAS CSCD 2022年第6期2981-2994,共14页
The purpose of this paper is to study the critical sand starting velocity and transformation law of flow pattern based on gas-water-sand three-phase flow in an inclined pipe.Firstly,the indoor simulation experiment sy... The purpose of this paper is to study the critical sand starting velocity and transformation law of flow pattern based on gas-water-sand three-phase flow in an inclined pipe.Firstly,the indoor simulation experiment system of gas-water-sand three-phase flow was used to test the conversion law of flow pattern based upon the different gas void fraction.Secondly,the influence of slug bubbles on sand migration was investigated according to distinctive hole deviation angles,gas void fraction and sand concentration.Finally,the critical sand starting velocity was tested based on dissimilar hole deviation angles,gas void fraction,sand concentration and sand particle size,and then the influence of the abovementioned key parameters on the sand starting velocity was debated based on the force analysis of the sand particles.The experimental results illustrated that when the gas void fraction was less than 5%,it was bubbly flow.When it increased from 5%to 30%,the bubbly flow and slug flow coexisted.When it was between 30%and 50%,the slug flow and agitated flow coexisted.When it reached 50%,it was agitated flow.Providing that the hole deviation angle was 90°,the phenomenon of overall migration and wavelike migration on the surface of sand bed was observed.On the contrary,the phenomenon of rolling and jumping migration was recognized.The critical sand starting velocity was positively correlated with the hole deviation angle and sand particle size,but negatively associated with the gas void fraction and sand concentration.This research can provide a certain reference for sand-starting production in the field of petroleum engineering. 展开更多
关键词 Three-phase flow Critical sand starting velocity flow pattern Sand migration Gas void fraction Hole deviation angles Sand concentration
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枫泾猪及其二元杂交F1代的营养价值评价
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作者 张佳杰 王洪洋 +7 位作者 刁玉段 吴昊旻 雷胜辉 薛云 孙杰 宋欣 谈永松 张莺莺 《食品工业科技》 北大核心 2026年第3期331-341,共11页
为探究枫泾猪(FJ)及其二元杂交F1代的营养价值,以FJ、杜(美)枫猪(DAF)、杜(挪)枫猪(DNF)、皮枫猪(PF)及杜长大猪(DLY)为研究对象,分析其氨基酸和脂肪酸的营养价值指标,并结合主成分分析综合评价其营养价值。结果表明,FJ及其杂交组合的总... 为探究枫泾猪(FJ)及其二元杂交F1代的营养价值,以FJ、杜(美)枫猪(DAF)、杜(挪)枫猪(DNF)、皮枫猪(PF)及杜长大猪(DLY)为研究对象,分析其氨基酸和脂肪酸的营养价值指标,并结合主成分分析综合评价其营养价值。结果表明,FJ及其杂交组合的总n-3多不饱和脂肪酸(Σ_(n-3)PUFA)含量显著高于DLY,且其必需氨基酸指数(EAAI)较高(86.32~90.66)。FJ的肌内脂肪含量(4.77%)、总多不饱和脂肪酸(ΣPUFA)含量(17.05%)和健康脂肪指数(1.83)均为最高,且具有较高的特征氨基酸含量。在不同杂交组合中,DNF的特征氨基酸含量最高;DAF的动脉粥样硬化指数(0.54)和血栓形成指数(1.31)最低;PF的EAAI(90.66)、营养指数(20.63)和生物价(87.12)最高,但其特征氨基酸含量最低。五组猪肉样本的第一限制氨基酸均为缬氨酸。营养价值综合评分为PF>DNF>FJ>DAF>DLY。综上所述,FJ及其杂交组合是摄取PUFA的优质食材,脂肪酸和蛋白质营养价值较高;PF为综合评价营养价值最高的杂交组合。 展开更多
关键词 枫泾猪 杂交f1代 脂肪酸 氨基酸 营养评价
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Effects of bump parameters on hypersonic inlet starting performance 被引量:1
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作者 Shang-cheng XU Yi WANG +2 位作者 Zhen-guo WANG Xiao-qiang FAN Bing XIONG 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2022年第10期807-819,共13页
Unstart is an unwanted flow phenomenon in a hypersonic inlet. When an unstart occurs, the captured airflow flowing through the engine significantly decreases with strong unsteady characteristics, which may lead to thr... Unstart is an unwanted flow phenomenon in a hypersonic inlet. When an unstart occurs, the captured airflow flowing through the engine significantly decreases with strong unsteady characteristics, which may lead to thrust loss or even combustor flameout. In this study, various bump configurations were designed to be integrated with a hypersonic inlet to improve its starting ability. A bump was defined as an integrated 3D compression surface installed upstream of the inlet entrance. The starting processes of these bump inlets were numerically simulated to investigate the effect laws and flow mechanisms of the bump parameters. Tests on bump height revealed that the starting performance could be significantly improved by increasing bump height, with the starting Mach number decreasing by 0.55 for the inlet with the highest bump. The high bump facilitates the side movement of the subsonic flow in the separation zone, which leads to a small separation bubble, thus accelerating the starting process. Further, the starting ability can be improved by designing a relatively wide bump, which results in a decline in the starting Mach number by 0.44. When the bump has the same or greater width compared with the airflow capture range, a growing spillage along the transverse direction can be formed so that the airflow in the separation bubble can be easily excluded, improving the starting ability. 展开更多
关键词 Hypersonic inlet BUMP Boundary layer flow starting performance Large-scale separation bubble
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Dynamics simulation of the belt conveyor possessing feedback loop during starting 被引量:1
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作者 席平原 张海涛 刘军 《Journal of Coal Science & Engineering(China)》 2005年第1期83-85,共3页
Synthesizing the mechanical models of the belt, the driver and the take-up device, the dynamics model was established on the longitudinal vibration of the overall belt conveyor system with finite elemental method, and... Synthesizing the mechanical models of the belt, the driver and the take-up device, the dynamics model was established on the longitudinal vibration of the overall belt conveyor system with finite elemental method, and S-function simulation block of asynchronous motor owing feedback function was built in Matlab/Simulink software, the simulation block indicates that motor rotation speed and its output moment vary with load and time, and the motor is a dynamic feedback system in working process. The state space block was adopted to express model of the belt. Thus it created simulation model of established dynamic model of overall belt conveyor system with Mat- lab/Simulink software, and simulates the course of starting by properly setting simulation parameters, and processes data for visualization. 展开更多
关键词 belt conveyor dynamics simulation starting process feedback loop
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Numerical investigation of dynamic characteristics of dual throat nozzle and bypass dual throat nozzle in thrust vectoring starting process 被引量:3
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作者 Yuqi ZHANG Jinglei XU +2 位作者 Minglei CAO Ruifeng PAN Shuai HUANG 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2024年第10期184-206,共23页
The Bypass Dual Throat Nozzle(BDTN)is a novel fluidic Thrust Vectoring(TV)nozzle,it switches to TV state by opening the valve in the bypass.To greatly manipulate the BDTN,the dynamic characteristics in the TV starting... The Bypass Dual Throat Nozzle(BDTN)is a novel fluidic Thrust Vectoring(TV)nozzle,it switches to TV state by opening the valve in the bypass.To greatly manipulate the BDTN,the dynamic characteristics in the TV starting process should be analyzed.This paper conducts numerical simulations to grasp the variation processes of performances and the flow field evolution of BDTN and Dual Throat Nozzle(DTN).The dynamic responses of TV starting in typical DTN models are investigated at first.Then,the TV starting processes of BDTN in different Nozzle Pressure Ratio(NPR)conditions are simulated,and the valve opening durations(T)are also considered.Before the expected TV direction is achieved in the DTN,the jet is deflected to the opposite direction at the beginning of the dynamic process,which is called the reverse TV phenomenon.However,this phenomenon disappears in the BDTN.The larger injection width of DTN intensifies unsteady oscillations,and the reverse TV phenomenon is strengthened.In the BDTN,T determines the delay degree of performance variations compared to the static results,which is called hysteresis effect.At NPR=10,the hysteresis affects the final stable performance of BDTN.This study analyses the dynamic characteristics in DTN and BDTN,laying a foundation for further design of nozzles and control strategies. 展开更多
关键词 Dual Throat Nozzle(DTN) Bypass Dual Throat Nozzle(BDTN) Dynamic characteristic Thrust vectoring starting process Cavity vortex Hysteresis effect
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牦牛源大肠杆菌F17菌毛A亚基和G亚基蛋白免疫保护效果比较分析
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作者 章石楠 韩生义 +8 位作者 石田 李淑萍 胡国元 高瑞 田嘉琪 周雯雯 屈亚鹏 陈逸雅 李生庆 《畜牧与兽医》 北大核心 2026年第2期61-70,共10页
为评价牦牛源大肠杆菌F17菌毛A亚基(F17A)和G亚基(F17G)的免疫原性,以本实验室分离的大肠杆菌QML2206-1为研究对象,采用生物信息学软件将F17A和F17G蛋白进行理化性质分析、跨膜结构和信号肽预测、二级结构和保守结构域预测;诱导表达并纯... 为评价牦牛源大肠杆菌F17菌毛A亚基(F17A)和G亚基(F17G)的免疫原性,以本实验室分离的大肠杆菌QML2206-1为研究对象,采用生物信息学软件将F17A和F17G蛋白进行理化性质分析、跨膜结构和信号肽预测、二级结构和保守结构域预测;诱导表达并纯化F17A和F17G蛋白;将纯化后的重组蛋白分为高(100μg/只)、低(50μg/只)剂量组免疫小鼠,利用ELISA方法检测小鼠特异性免疫球蛋白G(Ig G)抗体水平及血清中细胞因子簇分化抗原4(CD4)、簇分化抗原8(CD8)、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素4(IL-4)的表达水平,并通过小鼠攻毒保护试验评价其免疫保护效果。结果显示:2种蛋白均为稳定蛋白且具有信号肽区域;F17A蛋白无跨膜结构域,存在1个菌毛蛋白A(Fim A)保守结构域,F17G蛋白有跨膜结构域,存在Fim A和菌毛黏附素凝集素(Fim-adh-lectin) 2个保守结构域;2种蛋白的二级结构主要以无规则卷曲为主。经原核表达成功获得相对分子质量约为37和54 kDa的重组蛋白;重组蛋白免疫小鼠后,F17A高、低剂量组血清特异性Ig G抗体效价最高可达1∶32 000和1∶16 000,F17G高、低剂量组血清特异性Ig G抗体效价最高可达1∶32 000和1∶25 600;与对照组相比,免疫小鼠血清中CD4、CD8、TNF-α、IFN-γ及IL-4的表达水平均有所升高(P<0.05)。分别用最小致死量(MLD)、1.5 MLD、2 MLD、2.5 MLD攻毒小鼠后,除F17A高剂量免疫组最高仅能保护小鼠抵抗1.5 MLD攻毒菌量外,其余免疫组小鼠对4种攻毒菌量的存活率均为100%。综上,F17A和F17G重组蛋白具有良好的抗原性和一定的免疫保护作用。本研究为下一步基于F17蛋白的牦牛适用性大肠杆菌基因工程亚单位疫苗的研制提供了试验依据。 展开更多
关键词 牦牛源大肠杆菌 f17蛋白 原核表达 生物信息学分析 免疫原性
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Numerical Simulation of Air-Assisted Heating for Cold-Start in Cathode-Open Proton Exchange Membrane Fuel Cells
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作者 Wei Shi Shusheng Xiong +2 位作者 Wei Li Kai Meng Qingsheng Liu 《Energy Engineering》 2025年第9期3507-3523,共17页
In the realm of all-electric aircraft research,the integration of cathode-open proton exchange membrane fuel cells(PEMFC)with lithiumbatteries as a hybrid power source for small to medium-sized unmanned aerial vehicle... In the realm of all-electric aircraft research,the integration of cathode-open proton exchange membrane fuel cells(PEMFC)with lithiumbatteries as a hybrid power source for small to medium-sized unmanned aerial vehicles(UAVs)has garnered significant attention.The PEMFC,serving as the primary energy supply,markedly extends the UAV’s operational endurance.However,due to payload limitations and spatial constraints in the airframe layout of UAVs,the stack requires customized adaptation.Moreover,the implementation of auxiliary systems to facilitate cold starts of the PEMFC under low-temperature conditions is not feasible.Relying solely on thermal insulation measures also proves inadequate to address the challenges posed by complex low-temperature startup scenarios.To overcomethis,our study leverages the UAV’s lithium battery to heat the cathode inlet airflow,aiding the cathode-open PEMFC cold start process.To validate the feasibility of the proposed air-assisted heating strategy during the conceptual design phase,this study develops a transient,non-isothermal 3Dcathode-open PEMF Cunitmodel incorporating cathode air-assisted heating and gas-ice phase change.The model’s accuracy was verified against experimental cold-start data from a stack composed of identical single cells.This computational framework enables quantitative analysis of temperature fields and ice fraction distributions across domains under varying air-assisted heating powers during cold starts.Building upon this model,the study further investigates the improvement in cold start performance by heating the cathode intake air with varying power levels.The results demonstrate that the fuel cell achieves self-startup at temperatures as low as−13℃ under a constant current density of 100mA/cm^(2) without air-assisted heating.At an ambient temperature of−20℃,a successful start-up can be achieved with a heating power of 0.45 W/cm^(2).The temperature variation overtime during the cold start process can be represented by a sum of two exponential functions.The air-assisted heating scheme proposed in this study has significantly improved the cold start performance of fuel cells in low-temperature environments.Additionally,it provides critical reference data and validation support for component selection and feasibility assessment of hybrid power systems. 展开更多
关键词 PEMfC cold start numerical modeling air heating
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Evaluation System and Method of Industrial Park Renewal:A Case Study of the Starting Area of Handan City 被引量:1
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作者 ZHAO Ping GAO Ruihong +1 位作者 XUE Xiaona LI Wenya 《Journal of Landscape Research》 2022年第4期29-32,38,共5页
With the rapid development of urbanization,the industrial parks originally located in the suburbs are gradually incorporated into the urban center.Industries that fail to follow the transformation of urban economic de... With the rapid development of urbanization,the industrial parks originally located in the suburbs are gradually incorporated into the urban center.Industries that fail to follow the transformation of urban economic development and are forced to close,or enterprises that move out without conforming to the environment,become a gray area within the city.Most of the existing assessments are conducting single assessment of some indicators,but than their actual benefits.Through the field investigation on the starting area of Handan Economic Development Zone,and the evaluation on the nature of land use,development intensity,investment and output,and idle land,inefficient land use in the park is identified.According to different evaluation results,different update and promotion methods are put forward.The research could provide a new evaluation method and idea for subsequent renewal and development of urban industrial parks. 展开更多
关键词 Industrial park Inefficient land use Land use assessment starting area of Economic Development Zone
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Analysis of Starting Performance of High PowerFactor Induction Motor with Floating Windingin Parallel Connection with Capacitors
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作者 ZHANG Qi XIE Guo dong (School of Automation, Shanghai University) 《Advances in Manufacturing》 SCIE CAS 1999年第4期299-303,共5页
In this paper, by using a matrix technique, a dynamic model of high power factor induction motor with floating winding in parallel connection with capacitors is established. Then, the starting performance of this mot... In this paper, by using a matrix technique, a dynamic model of high power factor induction motor with floating winding in parallel connection with capacitors is established. Then, the starting performance of this motor is analyzed by computer simulation. By comparison of the tested and computed results, which are in good agreement, the dynamic model and simulative method are verified. 展开更多
关键词 high power factor starting performance computer simulation
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