<strong>Backgrounds:</strong> Cardiovascular diseases are still the prominent cause of death in cases of end-stage renal disease, cardiac troponin I (cTnI) can be used for detecting cardiac involvement in ...<strong>Backgrounds:</strong> Cardiovascular diseases are still the prominent cause of death in cases of end-stage renal disease, cardiac troponin I (cTnI) can be used for detecting cardiac involvement in asymptomatic cases of end-stage renal disease on hemodialysis. <strong>Aim:</strong> Determine the direct cardiac consequence of dialysis treatments in children on hemodialysis by measuring high-sensitive troponin-I as a marker of myocardial injury. <strong>Subjects and Methods:</strong> This case-control study included thirty children with end-stage renal disease on regular hemodialysis;the study group was selected from the nephrology hemodialysis unit of Al-Zahraa Hospital, Al-Azhar University. Another group of thirty healthy children matches age and sex with the patient’s group as a control. Highly Sensitive cTnI (hsTnI) was measured pre and post hemodialysis with a sensitive assay;moreover, ECG, lipid profile including cholesterol, triglyceride, low and high-density lipoprotein (HDL) in the same line with routine investigations for those patients, we used bioimpedance for dry weight assessment in the hemodialysis (HD) group. <strong>Results:</strong> Children on (HD) have a significantly higher (hsTnI) pre-dialysis (0.250 ± 0.069 ng/ml) compared to post-dialysis (0.187 ± 0.004 ng/ml) with (p, 0.001). With no significant difference between post HD (0.187 ± 0.004 ng/ml) and the control group (0.189 ± 0.005) with (p, 0.090). cTnI is detected in (73.3%) of children pre-dialysis above the cut-off value compared to (3.31%) had a high-level post-dialysis. cTnI is positively correlated with systolic, diastolic blood pressure and heart rate with (r. 0.333, p, 0.001: r. 0.343, p, 0.001: r. 0.276, p, 0.033) respectively and (hsTnI) is negatively correlated with Hb and HDL (r. -0.333, p, 0.009: r. 0.324, p, 0.011). Meanwhile (hsTnI) is positively correlated with serum urea, creatinine, ph, PTH, serum ferritin and positively correlated with QT interval and QTC. <strong>Conclusion:</strong> cTnI levels rise significantly before hemodialysis, so those patients are exposed to silent myocardial injury pre HD, and fortunately, it is not persistent after hemodialysis except for a few of them had a high level. We strongly advised not to delay dialysis appointments;the nephrology team should aggressively treat those patients to prevent further myocardial damage.展开更多
<strong>Introduction</strong><span style="font-family:Verdana;"><strong>: </strong></span><span style="font-family:Verdana;">According to Mali’s National ...<strong>Introduction</strong><span style="font-family:Verdana;"><strong>: </strong></span><span style="font-family:Verdana;">According to Mali’s National Immunization Center, the</span><span style="font-family:""> <i><span style="font-family:Verdana;">Hae</span><span style="font-family:Verdana;">mophilus influenzae</span></i><span style="font-family:Verdana;"> b (Hib) vaccine coverage rate was 90% in 2015. Our</span><span style="font-family:Verdana;"> work aimed to study invasive bacterial infections due to </span><i><span style="font-family:Verdana;">Haemophilus influenzae</span></i><span style="font-family:Verdana;"> type b in children aged 0</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">- 15 years hospitalized in the pediatrics department </span><span style="font-family:Verdana;">of the UH-GT</span></span><span style="font-family:Verdana;">. </span><b><span style="font-family:Verdana;">Method: </span></b><span style="font-family:Verdana;">We carried out a retrospective descriptive study</span><span style="font-family:""><span style="font-family:Verdana;"> from January 2017 to December 2018 (</span><i><span style="font-family:Verdana;">i</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">e</span></i><span style="font-family:Verdana;">. 2 years) among children aged 0</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">15 years and hospitalized for </span><i><span style="font-family:Verdana;">Haemophilus influenzae</span></i><span style="font-family:Verdana;"> type b infection confirmed by culture (blood culture, Cerebro-spinal Fluid, and pleural and skin fluid).</span></span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Results</span></b><b><span style="font-family:Verdana;">: </span></b><span style="font-family:""><span style="font-family:Verdana;">Thirty-three cases of Hib infections were collected giving a </span><span style="font-family:Verdana;">frequency of 0.2% and the age group 3 months to 3 years was the most</span><span style="font-family:Verdana;"> affected (72.73%).</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">Children who received no vaccine accounted for 21.21%.</span><span style="font-family:""> </span><span style="font-family:Verdana;">The Cerebro-spinal Fluid culture and other samples (pleural and skin) identified the </span><span style="font-family:Verdana;">bacterium</span><span style="font-family:""> </span><span style="font-family:Verdana;">in 100% of cases, against 72.72% in the blood culture</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">Meningitis </span><span style="font-family:Verdana;">was the most frequent pathology (78.79%) and the lethality was high</span><span style="font-family:Verdana;"> (21.21%).</span></span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> Despite the introduction of the Hib vaccine in the routine</span><span style="font-family:Verdana;"> immunization program in Mali, Hib infections remain with a high lethality linked to meningitis</span><span style="font-family:Verdana;">.</span>展开更多
<i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> inf...<i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> infection is estimated to cause 2.9 million diarrheal cases yearly among children aged under 24 months in sub-Saharan Africa. Studies have shown long-term climatic variations can affect infectious diseases. The burden of cryptosporidiosis in rural areas of sub-Saharan Africa is well characterized. However, the trend of </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection is not known, especially in informal urban settings. This study therefore sought to determine cryptosporidiosis trends, and further explore the association between year and </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection among children below 24 months in Kibera urban informal settlement in Kenya. Data collected by the Kenya Medical Research Institute longitudinal study in Tabitha clinic in Kibera from 2009 to 2015 were used. At least 3000 children aged < 24 months receive free health care at the clinic. In the longitudinal study</span></span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> children presenting with diarrhea were eligible for stool sample collection (</span><i><span style="font-family:Verdana;">n</span></i><span style="font-family:Verdana;"> = 477), out of which 421 stool samples were tested using TaqMan</span><span style="font-family:Verdana;"><span style="white-space:nowrap;">™</span></span><span style="font-family:Verdana;"> Array Card (TAC) polymerase chain reaction panel that included a target for </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> genus. Data for the 421 children were included in the analysis. Logistic regression was used to explore the difference between the seven years and cryptosporidiosis. Overall, the pooled data indicated that 23.5% of the children who were tested had </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection, with the highest proportions of </span><i><span style="font-family:Verdana;">Cryptosporidium-</span></i><span style="font-family:Verdana;">positive cases observed in 2015 (45.2%). The logistic regression results also indicated that children who were tested in the year 2015 were more likely to have </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection (OR = 3.39;95% CI: 1.44 - 7.96;</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.005) than those in 2009. Watery stool was also found to be an important symptom of cryptosporidiosis. There was a high prevalence of </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection among young children, especially in the most recent year. Routine testing of </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection using molecular methods, constant monitoring and identification of the infection sources is therefore necessary towards reducing the disease burden in the low resource settings.</span></span>展开更多
Panton-Valentine leukocidin (PVL) is one of the toxins responsible for increased virulence of <em>Staphylococcus aureus</em>. In school settings where children are in close contact with each other, <em&...Panton-Valentine leukocidin (PVL) is one of the toxins responsible for increased virulence of <em>Staphylococcus aureus</em>. In school settings where children are in close contact with each other, <em>S. aureus</em> strains, including those that may produce PVL, can be transmitted and spread in the community. Twenty-two multi-drug resistant MRSA nasal isolates from children enrolled in five schools in the town of Mariental and the multi-drug resistant American Type Culture Collection MRSA reference strain <em>S. aureus</em> ATCC 33591 (PVL-negative control) were used for molecular assays. Plasmid deoxyribonucleic acid (DNA) of isolates was amplified by polymerase chain reaction (PCR) and amplified PCR products were electrophoresed on a 2.5% (w/v) agarose gel containing 12 μl 0.5 μg/ml ethidium bromide and 1× TAE (Tris-acetate-EDTA) buffer at 90 volts for 50 minutes. The developed gel was viewed for the PVL-associated <em>lukS</em> and <em>lukF</em> genes that amplified at 151 bp and 406 bp, respectively. Our results indicated that seven nasal isolates had PVL toxin gene(s). From the seven isolates, three were tested positive for both <em>lukS</em> and<em> lukF</em>genes, one tested positive for only<em> lukS</em>, and three tested positive for only <em>lukF</em>. Two of the isolates harbouring both<em> lukS</em> and <em>lukF</em> genes shared the same antibiotic resistance pattern and one of them could also produce enterotoxin A. One of the isolates with only <em>lukF </em>gene could produce enterotoxins B and C. These toxin-producing isolates can be expected to be more virulent than non-producers. Children should be educated on the importance of regular handwashing with soap and water to prevent the spread of potentially virulent staphylococci amongst them and the wider community. This work warrants a larger study to be carried out to investigate PVL toxin and its associated infections in <em>Staphylococcus</em> from school children in Namibia.展开更多
<b><span style="font-family:Verdana;">Background and Purpose: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"...<b><span style="font-family:Verdana;">Background and Purpose: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;">Diarrhoeagenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (DEC) is one of the germs responsible for childhood diarrhea in developing countries. This study aims at determining the prevalence of the five main pathotypes of DEC isolated from faeces of children under five years old with diarrhea or not, living in the city of Koula-Moutou. </span><b><span style="font-family:Verdana;">Methodology: </span></b><span style="font-family:Verdana;">Isolates of </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> were phenotypically screened on chromID</span><sup><span style="font-family:Verdana;">TM</span></sup><span style="font-family:Verdana;"> agar and molecularly by multiplex PCR to detect the </span><span><span style="font-family:Verdana;">presence of enteroaggregative </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EAEC), enteropathogenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EPEC), </span></span><span style="font-family:Verdana;">enterotoxigenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (ETEC), enterohemorragic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EHEC) and enteroinvasive </span><i><span style="font-family:Verdana;">E. coli </span></i><span style="font-family:Verdana;">(EIEC). The evaluation of their sensitivity to 12 </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-lactam antibiotic molecules was carried out by Kirby Bauer method. This method has also made it possible to characterize phenotypically the different </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-lactamases produced. </span><b><span style="font-family:Verdana;">Results and Conclusion: </span></b><span style="font-family:Verdana;">Overall, at least one DEC pathovar was detected in the 63 </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> strains with phenotypic and molecular frequencies </span><span style="font-family:Verdana;">of 63.5% and 68.5% respectively. Thus, ETEC (28.3%) and EHEC (28.3%)</span><span style="font-family:Verdana;"> were the most frequent DEC in diarrheal isolates. ETEC/EHEC hybrid was recorded in both groups with rates of 7.5% in diarrheal cases and 10.0% for </span><span><span style="font-family:Verdana;">controls. The results showed produced carbapenemase type </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-lactamases</span></span><span style="font-family:Verdana;"> (31.7%), followed by ESBL (24.4%) and few produced high level penicillinases (4.9%). The DEC, in particular ETEC and EHEC are most likely the epidemiological agents responsible for childhood diarrhea in this study.</span></span>展开更多
文摘<strong>Backgrounds:</strong> Cardiovascular diseases are still the prominent cause of death in cases of end-stage renal disease, cardiac troponin I (cTnI) can be used for detecting cardiac involvement in asymptomatic cases of end-stage renal disease on hemodialysis. <strong>Aim:</strong> Determine the direct cardiac consequence of dialysis treatments in children on hemodialysis by measuring high-sensitive troponin-I as a marker of myocardial injury. <strong>Subjects and Methods:</strong> This case-control study included thirty children with end-stage renal disease on regular hemodialysis;the study group was selected from the nephrology hemodialysis unit of Al-Zahraa Hospital, Al-Azhar University. Another group of thirty healthy children matches age and sex with the patient’s group as a control. Highly Sensitive cTnI (hsTnI) was measured pre and post hemodialysis with a sensitive assay;moreover, ECG, lipid profile including cholesterol, triglyceride, low and high-density lipoprotein (HDL) in the same line with routine investigations for those patients, we used bioimpedance for dry weight assessment in the hemodialysis (HD) group. <strong>Results:</strong> Children on (HD) have a significantly higher (hsTnI) pre-dialysis (0.250 ± 0.069 ng/ml) compared to post-dialysis (0.187 ± 0.004 ng/ml) with (p, 0.001). With no significant difference between post HD (0.187 ± 0.004 ng/ml) and the control group (0.189 ± 0.005) with (p, 0.090). cTnI is detected in (73.3%) of children pre-dialysis above the cut-off value compared to (3.31%) had a high-level post-dialysis. cTnI is positively correlated with systolic, diastolic blood pressure and heart rate with (r. 0.333, p, 0.001: r. 0.343, p, 0.001: r. 0.276, p, 0.033) respectively and (hsTnI) is negatively correlated with Hb and HDL (r. -0.333, p, 0.009: r. 0.324, p, 0.011). Meanwhile (hsTnI) is positively correlated with serum urea, creatinine, ph, PTH, serum ferritin and positively correlated with QT interval and QTC. <strong>Conclusion:</strong> cTnI levels rise significantly before hemodialysis, so those patients are exposed to silent myocardial injury pre HD, and fortunately, it is not persistent after hemodialysis except for a few of them had a high level. We strongly advised not to delay dialysis appointments;the nephrology team should aggressively treat those patients to prevent further myocardial damage.
文摘<strong>Introduction</strong><span style="font-family:Verdana;"><strong>: </strong></span><span style="font-family:Verdana;">According to Mali’s National Immunization Center, the</span><span style="font-family:""> <i><span style="font-family:Verdana;">Hae</span><span style="font-family:Verdana;">mophilus influenzae</span></i><span style="font-family:Verdana;"> b (Hib) vaccine coverage rate was 90% in 2015. Our</span><span style="font-family:Verdana;"> work aimed to study invasive bacterial infections due to </span><i><span style="font-family:Verdana;">Haemophilus influenzae</span></i><span style="font-family:Verdana;"> type b in children aged 0</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">- 15 years hospitalized in the pediatrics department </span><span style="font-family:Verdana;">of the UH-GT</span></span><span style="font-family:Verdana;">. </span><b><span style="font-family:Verdana;">Method: </span></b><span style="font-family:Verdana;">We carried out a retrospective descriptive study</span><span style="font-family:""><span style="font-family:Verdana;"> from January 2017 to December 2018 (</span><i><span style="font-family:Verdana;">i</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">e</span></i><span style="font-family:Verdana;">. 2 years) among children aged 0</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">15 years and hospitalized for </span><i><span style="font-family:Verdana;">Haemophilus influenzae</span></i><span style="font-family:Verdana;"> type b infection confirmed by culture (blood culture, Cerebro-spinal Fluid, and pleural and skin fluid).</span></span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Results</span></b><b><span style="font-family:Verdana;">: </span></b><span style="font-family:""><span style="font-family:Verdana;">Thirty-three cases of Hib infections were collected giving a </span><span style="font-family:Verdana;">frequency of 0.2% and the age group 3 months to 3 years was the most</span><span style="font-family:Verdana;"> affected (72.73%).</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">Children who received no vaccine accounted for 21.21%.</span><span style="font-family:""> </span><span style="font-family:Verdana;">The Cerebro-spinal Fluid culture and other samples (pleural and skin) identified the </span><span style="font-family:Verdana;">bacterium</span><span style="font-family:""> </span><span style="font-family:Verdana;">in 100% of cases, against 72.72% in the blood culture</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">Meningitis </span><span style="font-family:Verdana;">was the most frequent pathology (78.79%) and the lethality was high</span><span style="font-family:Verdana;"> (21.21%).</span></span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> Despite the introduction of the Hib vaccine in the routine</span><span style="font-family:Verdana;"> immunization program in Mali, Hib infections remain with a high lethality linked to meningitis</span><span style="font-family:Verdana;">.</span>
文摘<i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:;" "=""><span style="font-family:Verdana;"> infection is estimated to cause 2.9 million diarrheal cases yearly among children aged under 24 months in sub-Saharan Africa. Studies have shown long-term climatic variations can affect infectious diseases. The burden of cryptosporidiosis in rural areas of sub-Saharan Africa is well characterized. However, the trend of </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection is not known, especially in informal urban settings. This study therefore sought to determine cryptosporidiosis trends, and further explore the association between year and </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection among children below 24 months in Kibera urban informal settlement in Kenya. Data collected by the Kenya Medical Research Institute longitudinal study in Tabitha clinic in Kibera from 2009 to 2015 were used. At least 3000 children aged < 24 months receive free health care at the clinic. In the longitudinal study</span></span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> children presenting with diarrhea were eligible for stool sample collection (</span><i><span style="font-family:Verdana;">n</span></i><span style="font-family:Verdana;"> = 477), out of which 421 stool samples were tested using TaqMan</span><span style="font-family:Verdana;"><span style="white-space:nowrap;">™</span></span><span style="font-family:Verdana;"> Array Card (TAC) polymerase chain reaction panel that included a target for </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> genus. Data for the 421 children were included in the analysis. Logistic regression was used to explore the difference between the seven years and cryptosporidiosis. Overall, the pooled data indicated that 23.5% of the children who were tested had </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection, with the highest proportions of </span><i><span style="font-family:Verdana;">Cryptosporidium-</span></i><span style="font-family:Verdana;">positive cases observed in 2015 (45.2%). The logistic regression results also indicated that children who were tested in the year 2015 were more likely to have </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection (OR = 3.39;95% CI: 1.44 - 7.96;</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.005) than those in 2009. Watery stool was also found to be an important symptom of cryptosporidiosis. There was a high prevalence of </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection among young children, especially in the most recent year. Routine testing of </span><i><span style="font-family:Verdana;">Cryptosporidium</span></i><span style="font-family:Verdana;"> infection using molecular methods, constant monitoring and identification of the infection sources is therefore necessary towards reducing the disease burden in the low resource settings.</span></span>
文摘Panton-Valentine leukocidin (PVL) is one of the toxins responsible for increased virulence of <em>Staphylococcus aureus</em>. In school settings where children are in close contact with each other, <em>S. aureus</em> strains, including those that may produce PVL, can be transmitted and spread in the community. Twenty-two multi-drug resistant MRSA nasal isolates from children enrolled in five schools in the town of Mariental and the multi-drug resistant American Type Culture Collection MRSA reference strain <em>S. aureus</em> ATCC 33591 (PVL-negative control) were used for molecular assays. Plasmid deoxyribonucleic acid (DNA) of isolates was amplified by polymerase chain reaction (PCR) and amplified PCR products were electrophoresed on a 2.5% (w/v) agarose gel containing 12 μl 0.5 μg/ml ethidium bromide and 1× TAE (Tris-acetate-EDTA) buffer at 90 volts for 50 minutes. The developed gel was viewed for the PVL-associated <em>lukS</em> and <em>lukF</em> genes that amplified at 151 bp and 406 bp, respectively. Our results indicated that seven nasal isolates had PVL toxin gene(s). From the seven isolates, three were tested positive for both <em>lukS</em> and<em> lukF</em>genes, one tested positive for only<em> lukS</em>, and three tested positive for only <em>lukF</em>. Two of the isolates harbouring both<em> lukS</em> and <em>lukF</em> genes shared the same antibiotic resistance pattern and one of them could also produce enterotoxin A. One of the isolates with only <em>lukF </em>gene could produce enterotoxins B and C. These toxin-producing isolates can be expected to be more virulent than non-producers. Children should be educated on the importance of regular handwashing with soap and water to prevent the spread of potentially virulent staphylococci amongst them and the wider community. This work warrants a larger study to be carried out to investigate PVL toxin and its associated infections in <em>Staphylococcus</em> from school children in Namibia.
文摘<b><span style="font-family:Verdana;">Background and Purpose: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;">Diarrhoeagenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (DEC) is one of the germs responsible for childhood diarrhea in developing countries. This study aims at determining the prevalence of the five main pathotypes of DEC isolated from faeces of children under five years old with diarrhea or not, living in the city of Koula-Moutou. </span><b><span style="font-family:Verdana;">Methodology: </span></b><span style="font-family:Verdana;">Isolates of </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> were phenotypically screened on chromID</span><sup><span style="font-family:Verdana;">TM</span></sup><span style="font-family:Verdana;"> agar and molecularly by multiplex PCR to detect the </span><span><span style="font-family:Verdana;">presence of enteroaggregative </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EAEC), enteropathogenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EPEC), </span></span><span style="font-family:Verdana;">enterotoxigenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (ETEC), enterohemorragic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EHEC) and enteroinvasive </span><i><span style="font-family:Verdana;">E. coli </span></i><span style="font-family:Verdana;">(EIEC). The evaluation of their sensitivity to 12 </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-lactam antibiotic molecules was carried out by Kirby Bauer method. This method has also made it possible to characterize phenotypically the different </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-lactamases produced. </span><b><span style="font-family:Verdana;">Results and Conclusion: </span></b><span style="font-family:Verdana;">Overall, at least one DEC pathovar was detected in the 63 </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> strains with phenotypic and molecular frequencies </span><span style="font-family:Verdana;">of 63.5% and 68.5% respectively. Thus, ETEC (28.3%) and EHEC (28.3%)</span><span style="font-family:Verdana;"> were the most frequent DEC in diarrheal isolates. ETEC/EHEC hybrid was recorded in both groups with rates of 7.5% in diarrheal cases and 10.0% for </span><span><span style="font-family:Verdana;">controls. The results showed produced carbapenemase type </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-lactamases</span></span><span style="font-family:Verdana;"> (31.7%), followed by ESBL (24.4%) and few produced high level penicillinases (4.9%). The DEC, in particular ETEC and EHEC are most likely the epidemiological agents responsible for childhood diarrhea in this study.</span></span>
