Objective：To investigate the effect of nifedipine（calciumchannel blocker） on the expression of collagen in gingival fibroblasts invitro. Methods：Primarily gingival fibroblasts were cultured and incubated with vari...Objective：To investigate the effect of nifedipine（calciumchannel blocker） on the expression of collagen in gingival fibroblasts invitro. Methods：Primarily gingival fibroblasts were cultured and incubated with various concentrations of nifedipine（108 μg/L, 360 μg/L and 1200 μg/L）for 5 days. Gingival fibroblasts were primarily cultured derived from nifedipine responders and nonresponders in the presence of 360 μg/L nifedipine. Enzyme-linked immunosorbent assay was used to evaluate the amount of type I collagen. Cell proliferation was measured by cell counting with evaluating MTT value. Results：The expressions of collagen and cell proliferation were significantly different among the high concentration groups and the others on the fifth day, especially higher in 360 μg/L and 1200 μg/L groups and also different among nifedipine responders and non-responders. Conclusion：The expression of collagen and cell proliferation may be concerned with the biological mechanism for gingival overgrowth.展开更多
Glycogen storage disease type Ia (GSD-Ia; also called von Gierke disease) is an autosomal recessive disorder of carbohydrate metabolism caused by glucose-6-phosphatase deficiency. There have been many reports describi...Glycogen storage disease type Ia (GSD-Ia; also called von Gierke disease) is an autosomal recessive disorder of carbohydrate metabolism caused by glucose-6-phosphatase deficiency. There have been many reports describing hepatic tumors in GSD patients; however, most of these reports were of hepatocellular adenomas, whereas there are only few reports describing focal nodular hyperplasia (FNH) or hepatocellular carcinoma (HCC). We report a case with GSD-Ia who had undergone a partial resection of the liver for FNH at 18 years of age and in whom moderately differentiated HCC had developed. Preoperative imaging studies, including ultrasonography, dynamic computer tomography (CT) and magnetic resonance imaging, revealed benign and malignant features. In particular, fluorodeoxyglucose-positron emission tomography (FDG-PET)/CT revealed the atypical findings that FDG accumulated at high levels in the non-tumorous hepatic parenchyma and low levels in the tumor. Right hemihepatectomy was performed. During the perioperative period, high-dose glucose and sodium bicarbonate were administered to control metabolic acidosis. He had multiple recurrences of HCC at 10 mo after surgery and was followed-up with transcatheter arterial chemoembolization. The tumor was already highly advanced when it was found by chance; therefore, a careful follow-up should be mandatory for GSD-I patients as they are at a high risk for HCC, similar to hepatitis patients.展开更多
The transcription factor forkhead box protein A2(FOXA2, also known as hepatocyte nuclear factor 3β or transcription factor 3β), has been found to play pivotal roles in multiple phases of mammalian life, from the ear...The transcription factor forkhead box protein A2(FOXA2, also known as hepatocyte nuclear factor 3β or transcription factor 3β), has been found to play pivotal roles in multiple phases of mammalian life, from the early development to the organofaction, and subsequently in homeostasis and metabolism in the adult. In the embryonic development period, FOXA2 is require d for the formation of the primitive node and notochord, and its absence results in embryonic lethality. Moreover, FOXA2 plays an important role not only in lung development, but also in T helper type 2(Th2)-mediated pulmonary inflammation and goblet cell hyperplasia. In this article, the role of FOXA2 in lung development and Th2-mediated pulmonary inflammation, as well as in goblet cell hyperplasia, is reviewed. FOXA2 deletion in airway epithelium results into Th2-mediated pulmonary inflammation and goblet cell hyperplasia in developing lung. Leukotriene pathway and signal transducers and activators of transcription 6 pathway may mediate this inflammation through recruitment and activation of denditric cell during lung developments. FOXA2 is a potential treatment target for lung diseases with Th2 inflammation and goblet cell hyperplasia, such as asthma and chronic obstructive pulmonary disease.展开更多
目的探讨2型糖尿病(T2DM)与高龄老年人良性前列腺增生症(bengn prostatic hyperplasia,BPH)的关系。方法回顾性分析167例高龄BPH患者的临床资料,其中单纯BPH患者64例,T2DM合并BPH患者103例。收集血压、空腹血糖(FBG)、餐后2 h血糖(2 h P...目的探讨2型糖尿病(T2DM)与高龄老年人良性前列腺增生症(bengn prostatic hyperplasia,BPH)的关系。方法回顾性分析167例高龄BPH患者的临床资料,其中单纯BPH患者64例,T2DM合并BPH患者103例。收集血压、空腹血糖(FBG)、餐后2 h血糖(2 h PBG)、空腹胰岛素(FINS)、糖化血红蛋白(HbAlc)、血脂、血清前列腺特异性抗原(PSA)、国际前列腺症状评分(IPSS)及经腹部超声测量的前列腺体积(PV)等临床资料。结果单纯BPH组和T2DM合并BPH组,两组年龄、合并高血压及高脂血症情况比较,差异无统计学意义(P>0.05);与单纯BPH组相比,BPH合并T2DM组患者血清FINS、PSA、PV水平及IPSS评分显著升高(P<0.05)。BPH合并T2DM组中,不同水平FBG、2 h PBG患者的IPSS评分、PV与血清PSA,差异无统计学意义(P>0.05);BPH合并T2DM组中,HbA1c升高患者与HbA1c正常者比较,IPSS评分、PV与血清PSA明显升高(P<0.05)。结论T2DM是高龄患者BPH发生与进展的危险因素。展开更多
基金suppored by the Natural Science Foundation of the Department of Education of Jiangsu(BK2006172)
文摘Objective：To investigate the effect of nifedipine（calciumchannel blocker） on the expression of collagen in gingival fibroblasts invitro. Methods：Primarily gingival fibroblasts were cultured and incubated with various concentrations of nifedipine（108 μg/L, 360 μg/L and 1200 μg/L）for 5 days. Gingival fibroblasts were primarily cultured derived from nifedipine responders and nonresponders in the presence of 360 μg/L nifedipine. Enzyme-linked immunosorbent assay was used to evaluate the amount of type I collagen. Cell proliferation was measured by cell counting with evaluating MTT value. Results：The expressions of collagen and cell proliferation were significantly different among the high concentration groups and the others on the fifth day, especially higher in 360 μg/L and 1200 μg/L groups and also different among nifedipine responders and non-responders. Conclusion：The expression of collagen and cell proliferation may be concerned with the biological mechanism for gingival overgrowth.
文摘Glycogen storage disease type Ia (GSD-Ia; also called von Gierke disease) is an autosomal recessive disorder of carbohydrate metabolism caused by glucose-6-phosphatase deficiency. There have been many reports describing hepatic tumors in GSD patients; however, most of these reports were of hepatocellular adenomas, whereas there are only few reports describing focal nodular hyperplasia (FNH) or hepatocellular carcinoma (HCC). We report a case with GSD-Ia who had undergone a partial resection of the liver for FNH at 18 years of age and in whom moderately differentiated HCC had developed. Preoperative imaging studies, including ultrasonography, dynamic computer tomography (CT) and magnetic resonance imaging, revealed benign and malignant features. In particular, fluorodeoxyglucose-positron emission tomography (FDG-PET)/CT revealed the atypical findings that FDG accumulated at high levels in the non-tumorous hepatic parenchyma and low levels in the tumor. Right hemihepatectomy was performed. During the perioperative period, high-dose glucose and sodium bicarbonate were administered to control metabolic acidosis. He had multiple recurrences of HCC at 10 mo after surgery and was followed-up with transcatheter arterial chemoembolization. The tumor was already highly advanced when it was found by chance; therefore, a careful follow-up should be mandatory for GSD-I patients as they are at a high risk for HCC, similar to hepatitis patients.
基金The National Natural Science Foundation of China,Nos.30871118,30971325,81270129 and 81470268(FL)grants from Department of Science and Technology of Sichuan Province,Nos.09ZQ026-020 and 2009SZ0190(FL)
文摘The transcription factor forkhead box protein A2(FOXA2, also known as hepatocyte nuclear factor 3β or transcription factor 3β), has been found to play pivotal roles in multiple phases of mammalian life, from the early development to the organofaction, and subsequently in homeostasis and metabolism in the adult. In the embryonic development period, FOXA2 is require d for the formation of the primitive node and notochord, and its absence results in embryonic lethality. Moreover, FOXA2 plays an important role not only in lung development, but also in T helper type 2(Th2)-mediated pulmonary inflammation and goblet cell hyperplasia. In this article, the role of FOXA2 in lung development and Th2-mediated pulmonary inflammation, as well as in goblet cell hyperplasia, is reviewed. FOXA2 deletion in airway epithelium results into Th2-mediated pulmonary inflammation and goblet cell hyperplasia in developing lung. Leukotriene pathway and signal transducers and activators of transcription 6 pathway may mediate this inflammation through recruitment and activation of denditric cell during lung developments. FOXA2 is a potential treatment target for lung diseases with Th2 inflammation and goblet cell hyperplasia, such as asthma and chronic obstructive pulmonary disease.
文摘目的探讨2型糖尿病(T2DM)与高龄老年人良性前列腺增生症(bengn prostatic hyperplasia,BPH)的关系。方法回顾性分析167例高龄BPH患者的临床资料,其中单纯BPH患者64例,T2DM合并BPH患者103例。收集血压、空腹血糖(FBG)、餐后2 h血糖(2 h PBG)、空腹胰岛素(FINS)、糖化血红蛋白(HbAlc)、血脂、血清前列腺特异性抗原(PSA)、国际前列腺症状评分(IPSS)及经腹部超声测量的前列腺体积(PV)等临床资料。结果单纯BPH组和T2DM合并BPH组,两组年龄、合并高血压及高脂血症情况比较,差异无统计学意义(P>0.05);与单纯BPH组相比,BPH合并T2DM组患者血清FINS、PSA、PV水平及IPSS评分显著升高(P<0.05)。BPH合并T2DM组中,不同水平FBG、2 h PBG患者的IPSS评分、PV与血清PSA,差异无统计学意义(P>0.05);BPH合并T2DM组中,HbA1c升高患者与HbA1c正常者比较,IPSS评分、PV与血清PSA明显升高(P<0.05)。结论T2DM是高龄患者BPH发生与进展的危险因素。
