Atherosclerosis remains a great threat to human health worldwide.Previous studies found that tetramethylpyrazine(TMP)and paeonifl orin(PF)combination(TMP-PF)exerts anti-atherosclerotic effects in vitro.However,whether...Atherosclerosis remains a great threat to human health worldwide.Previous studies found that tetramethylpyrazine(TMP)and paeonifl orin(PF)combination(TMP-PF)exerts anti-atherosclerotic effects in vitro.However,whether TMP-PF improves atherosclerosis in vivo needs further exploration.The present study aims to assess the anti-atherosclerotic properties of TMP-PF in ApoE^(-/-)mice and explore the related molecule mechanisms.Results showed that TMP and high-dose TMP-PF decreased serum triglyceride and low-density lipoprotein cholesterol levels,suppressed vascular endothelial growth factor receptor 2(VEGFR2)and nuclear receptor subfamily 4 group A member 1(NR4A1)expression in aortic tissues,inhibited plaque angiogenesis,reduced plaque areas,and alleviated atherosclerosis in ApoE^(-/-)mice.Also,TMP-PF exhibited a better modulation effect than TMP or PF alone.However,NR4A1 agonist abolished the anti-atherosclerotic effects of TMP-PF.In conclusion,TMP-PF was first found to alleviate atherosclerosis progression by reducing hyperlipemia and inhibiting plaque angiogenesis via the NR4A1/VEGFR2 pathway,indicating that TMP-PF had a positive effect on reducing hyperlipemia and attenuating atherosclerosis development.展开更多
Objective To observe therapeutic effects of acupuncture on the mouse of hyperlipemia and to explore the mechanisms. Methods One hundred and twenty Kunming mice, male, sanitary degree, were randomly divided into normal...Objective To observe therapeutic effects of acupuncture on the mouse of hyperlipemia and to explore the mechanisms. Methods One hundred and twenty Kunming mice, male, sanitary degree, were randomly divided into normal group （n=40）, a model group （n=40）, an acupuncture group （n=20） and a medicine group （n=20）. Except the normal group, the mice were fed with high fat forage to prepare mouse hyperlipemia model. On the 15th day of modeling, serum total cholesterol （TC）, triglyceride （TG）, low density lipoprotein-cholesterol （LDL-C）, high density lipoprotein-cholesterol （HDL-C） levels were detected in 20 normal mice and 20 model mice; electroacupuncture was given at bilateral ＂Fēnglóng＂ （丰隆 ST 40）, ＂Qūchí＂ （曲池 LI 11）, ＂Sānyīnjiāo＂ （三阴交 SP 6） in the acupuncture group, once a day, for 10 consecutive days; in the medicine group, the mice were intragastrically administrated with Simvastatin, once daily , for 10 consecutive days. After the end of treatment, serum TC, TG, LDL-C, HDL-C, malondialdehyde （MDA）, nitric oxide （NO） and endothelin （ET） contents and superoxide dismutase （SOD） activities were detected in the groups. Results On the 15th day of modeling, in the model group serum TG, TC, LDL-C contents were significantly higher （all P〈0.05） and HDL-C content was significantly lower （P〈0.01） than those in the normal group. After treatment for 10 days, in the acupuncture group and the medicine group serum TG, TC, LDL-C, MDA and ET contents were significantly lower and serum HDL-C and NO contents and SOD activities were significantly higher than those in the model group （P〈0.05, P〈0.01）, and the improving action in the acupuncture group was better than that in the medicine group （P〈0.05, P〈0.01）. Conclusion Acupuncture can regulate fat metabolism, resist lipid peroxidation and protect vascular endothelial function in the mouse of hyperlipemia.展开更多
Objective To explore the mechanism of electroacupuncture（EA） at ＂Fēnglóng＂（丰隆 ST 40） for hyperlipemia（HLP） in rats.Methods Forty health SD rats were randomly divided into a normal control group（group ...Objective To explore the mechanism of electroacupuncture（EA） at ＂Fēnglóng＂（丰隆 ST 40） for hyperlipemia（HLP） in rats.Methods Forty health SD rats were randomly divided into a normal control group（group A）,a high fat forage group（group B） and a high fat forage ＋ treatment group（group C）,a high fat forage ＋ normal forage group（group D） and a high fat forage ＋ normal forage ＋ treatment group（group E）,eight rats in each group.EA was applied at ＂Fēnglóng＂（丰隆 ST 40） of the rats in group C and group E,once daily.After treatment of 30 days,plasma lipid levels in the rats,including total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C） were detected.Real-time quantitative polymerase chain reaction（RT-PCR） and Western Blotting were applied to detect the gene expression changes of ATP-binding cassette transporter A1（ABCA1）,peroxisome proliferator-activated receptor alpha（PPARα）,liver X receptor alpha（LXR-α） and retinoid X receptor alpha（RXR-α） in liver tissue of rats.Results Compared with group A,contents of TC,LDL-C were significantly elevated in group B and group D（all P〈0.01）;compared with group B,above indices were significantly decreased in group D（all P〈0.01）.After the treatment of EA at ＂Fēnglóng＂（丰隆 ST 40）,contents of TC,LDL-C were significantly decreased（all P〈0.01）,and contents of TG,HDL-C did not change significantly（all P〉0.05）.Compared with group A,mRNA and protein contents of ABCA1,PPARα,LXR-α and RXR-α were decreased significantly in group B and group D（all P〈0.01）.But compared with group B,the above indices were decreased in group D.After the treatment of EA,mRNA and protein contents of ABCA1,PPARα,RXR-α and LXR-α significantly increased（all P〈0.05）.Conclusion EA at ＂Fēnglóng＂（丰隆 ST 40） can decrease contents of blood TC,LDL-C in rats of hyperlipemia and up-regulate the gene expression of ABCA1,PPARα,LXR-α and RXR-α in liver,so as to promote reverse cholesterol transport,with a certain therapeutic effect on hyperlipemia.展开更多
In the present paper, the effect of point-injection of Radix Salviae Miltiorrhizae injectio on blood-lipid was observed in 65 cases of hyperlipemia. The 65 patients were divided into point-injection group (40 cases wh...In the present paper, the effect of point-injection of Radix Salviae Miltiorrhizae injectio on blood-lipid was observed in 65 cases of hyperlipemia. The 65 patients were divided into point-injection group (40 cases who were treated with point-injection of Radix Salviae Miltiorrhizae injectio and oral administration of Shujiangzhi) and control group (25 cases who were treated with oral administration of Shujiangzhi only). Results showed that the total effective rate of the point-injection group was significantly higher than that of the control group (P<0. 05); point-injection of Radix Salviae Miltiorrhizae injectio could effectively raise the level of HDL-C, decrease lower LDL-C and regulate estrogen level of women. The slow releasing action of red sage root solution in the topical acupoint region prolonged and enhanced its effect in lowering blood-lipid. There was a significant difference between the two groups in the effect of lowering blood-fat (P< 0. 01).展开更多
In this experiment, the contents of serum total cholesterol (TC), triglyceride(TG),low-density lipoprotein(LDL), high-density lipoprotein(HDL) were used as indexes to observe the effects of warming-reinforcing method ...In this experiment, the contents of serum total cholesterol (TC), triglyceride(TG),low-density lipoprotein(LDL), high-density lipoprotein(HDL) were used as indexes to observe the effects of warming-reinforcing method on rabbit’s lipide, liopoprotein. The results indicated that in the warming-reinforcing group, TC decreased markedly and HDL increased obviously with significant difference as compared with the twirling-reinforcing group:TC and LDL also decreased, but no significant differencte as compared with the twirling-reinforcing group. Warming-reinforcing method can decrease lipids more effectively than twirling-reinforcing method.展开更多
BACKGROUND Severe hyperlipemia(SHLE)has an impact on the results of many kinds of laboratory tests.Complete blood count(CBC)examination by automated blood cell counter(ABCC)is a quick and convenient measurement for sc...BACKGROUND Severe hyperlipemia(SHLE)has an impact on the results of many kinds of laboratory tests.Complete blood count(CBC)examination by automated blood cell counter(ABCC)is a quick and convenient measurement for screening abnormalities of blood cells that are triggered by various pathogenic insults in disease diagnosis and for monitoring changes in the treatment of existing hematological conditions.However,CBC results are frequently affected by many intrinsic and extrinsic factors from blood samples,such as in the setting of hypergammaglobulinemia and certain anticoagulants.SHLE could also affect CBC results.CASE SUMMARY A 33-year-old Chinese male presented with painful foot numbness and abdominal pain.He was initially misdiagnosed as having a myeloproliferative neoplasm(MPN)because of the marked abnormalities in CBC examination by the ABCC.Morphological evaluation of the bone marrow smears and biopsy showed no evidence of MPN.Gene mutations in Breakpoint cluster regions-Abelson murine leukemia viral oncogene homologue 1(BCR-ABL1),Janus kinase 2(JAK2),calreticulin(CALR),myeloproliferative leukemia virus(MPL),and colony-stimulating factor 3 receptor(CSF3R)were negative.Having noticed the thick chylomicron layer on blood samples and the dramatically fluctuating CBC results,we speculated that the fat droplets formed by shaking the blood samples in the setting of SHLE were mistakenly identified as blood cells due to the limited parameters of ABCC.Therefore,we removed a large part of the chylomicron layer and then reexamined the CBC,and the CBC results,as we expected,differed significantly from that of the sample before the chylomicron layer was removed.These significant differences had been validated by the subsequently repeated laboratory tests by measuring dual blood samples that the chylomicron layer was removed in one sample and was not in another,and comparing the CBC results.Computerized tomography reexamination of the upper abdomen revealed an exudative lesion surrounding his pancreas.After intensive consultation,definitive diagnosis was made as recurrent pancreatitis,hyperlipemia and pseudoerythrocytosis.CONCLUSION SHLE may become a potential cause of misdiagnosis of hyperlipemia-related diseases as MPNs and the resultant mistreatment.It may also lead to the misinterpretation of transfusion indications in patients with hematological disorders who critically need blood transfusion for supportive treatment.展开更多
Hyperlipemia is a complication commonly seen in non-insulin-dependent diabetes(NIDDM).This complication can causeischemic heart disease,cerebro-vascular disease and even lifethreatening.In order to prevent this compli...Hyperlipemia is a complication commonly seen in non-insulin-dependent diabetes(NIDDM).This complication can causeischemic heart disease,cerebro-vascular disease and even lifethreatening.In order to prevent this complication and to reduce mortality of NIDDM,60patients with NIDDMand hyperlipemia were investigated.Alimentary therapy consisting of the following items was given:(1)routine control of total calorie consumption:(2)carbohydrates,lipids,proteins,vitamins and minerals taken in proportional amounts;(3)fish proteins were the main proteins in the diet;(4)dried mushroom(3g/day);(5)little tea after meal;(6)corn meal or millet gruel(5-10g./day).With this dietary regime,majority of the NIDDMpatiens with serum total lipid returned to normal or near normal.展开更多
Objective： To observe the therapeutic effect of antihyperlipidemic decoction on hyperlipemia. Methods： 77 hyperlipemia patients are selected and randomly divided into two groups. 37 cases in the treatment group rece...Objective： To observe the therapeutic effect of antihyperlipidemic decoction on hyperlipemia. Methods： 77 hyperlipemia patients are selected and randomly divided into two groups. 37 cases in the treatment group received the treatment of antihyperlipidemic decoction, and 40 cases in the control group received western medicine, zhinbiticose. The course of treatment for both groups was 8 weeks. Results： The difference in total effective rate between the two groups was significant （P〈0.05）. Conclusion： Antihyperlipidemic decoction has a better therapeutic effect for hvoerlioemia, with a lower recurrence rate and less side effect.展开更多
Objective: To observe the effect of herb-medicine-cake-separated moxibustion on serum lipoprotein in hyperlipemia rabbits. Methods: 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (...Objective: To observe the effect of herb-medicine-cake-separated moxibustion on serum lipoprotein in hyperlipemia rabbits. Methods: 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14), direct moxibustion group (n=14) and herb-medicine-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% lard, 0.5% cholesterol and common forage) for 6 weeks. Moxibustion was applied to “Juque”(CV 14), “Tianshu”(ST 25), “Fenglong”(ST 40), etc., 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum high density lipoprotein-cholesterol (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch) and total cholesterol (TCh) contents were assayed with colorimetric method. Results: Compared with control group, serum LDL-Ch content, HDL-Ch/LDL-Ch and HDL-Ch/TCh of model group were significantly higher (P<0.05～0.01), while compared with model group, LDL-Ch contents of two moxibustion groups were strikingly lower (P<0.01). No significant differences were found between two moxibustion groups in all the 4 indexes. Conclusion: Both direct and indirect moxibustion can effectively lower serum LDL-Ch, raise HDL-Ch, HDL-Ch/LDL-Ch and HDL-Ch/TCh, and regulate lipoprotein metabolism in hyperlipemia rabbits.展开更多
Objective: To study the effect of medicinal herb-cake-separated moxibustion on serum lipoprotein levels in hyperlipemia rabbits. Methods: A total of 55 New-Zealand rabbits were randomly divided into control group (n=1...Objective: To study the effect of medicinal herb-cake-separated moxibustion on serum lipoprotein levels in hyperlipemia rabbits. Methods: A total of 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14), direct moxibustion group (n=14) and medicinal herb-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% lard, 0.5% cholesterol and common forage) for 6 weeks. Moxibustion was applied to “Juque”(CV 14), “Tianshu”(ST 25), “Fenglong”(ST 40), etc., 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum triglyceride (TG) and total cholesterol (TCh) contents were assayed with colorimentric method. Results: Compared with control group, TCh and TG levels of model group increased significantly (P< 0.01). TCh and TG contents of direct moxibustion and indirect moxibustion groups were significantly lower than those of model group (P<0.01). Comparison between two moxibustion groups showed that serum TCh level of indirect moxibustion group was strikingly lower than that of direct moxibustion group (P<0.01). It indicated that both direct and indirect moxibustion could effectively lower hyperlipemia and the therapeutic effect of indirect moxibustion was significantly superior to that of direct moxibustion in lowering serum TCh level. Conclusion: Both direct and indirect moxibustion can regulate lipid metabolism and the therapeutic effect of medicinal herb-cake-separated moxibustion is superior to that of direction moxibustion in hyperlipemia rabbits.展开更多
Objective: To observe effects of herbal cake-separated moxibustion on blood lipids, including total cholesterol (TCh), triglyceride (TG), high density lipoprotein-Ch (HDL-Ch), low density lipoprotein-Ch (LDL-Ch), apol...Objective: To observe effects of herbal cake-separated moxibustion on blood lipids, including total cholesterol (TCh), triglyceride (TG), high density lipoprotein-Ch (HDL-Ch), low density lipoprotein-Ch (LDL-Ch), apolipoprotein A (Apo A), apolipoprotein B (Apo B), and plasma thromboxane B2 (TXB2) and 6-keto-prostaglandin F 1α (6-keto-PGF 1α) contents and analyse its mechanism. Methods: The hyperlipemia rabbit model was established by breeding of high fat forage and injection of bovine serum albumin. They were treated respectively by direct moxibustion and herbal cake-separated moxibustion at Juque (巨阙 CV 14), Tianshu (天枢 ST 25), Fenglong (丰隆 ST 40), etc., once daily, continuously for 40 days. Blood TCh and TG contents were detected with enzymatic method, LDL-Ch and HDL-Ch with colorimetric method, Apo A and Apo B with electrophoretic method, and TXB2 and 6-keto-PGF 1α with radioimmunoassay. Results: Both the herbal cake-separated moxibustion and direct moxibustion could effectively decrease TCh, TG, LDL-Ch, Apo B and TXB2 contents and TXB2/6-keto-PGF 1α, and increase HDL-CH and 6-keto-PGF 1α contents in the rabbit of hyperlipemia. Conclusion: 6-keto-PGF 1α and TXB2 are possibly involved in the mechanism of herbal cake-separated moxibustion decreasing blood lipids.展开更多
Objective To observe the effects of therapeuti c ultrasound in vitro on hyperlipemi a.Methods Comparing clinical effects of only ultrasound treatment,only adminis tration of Geminifbrozip and combin ation of these two...Objective To observe the effects of therapeuti c ultrasound in vitro on hyperlipemi a.Methods Comparing clinical effects of only ultrasound treatment,only adminis tration of Geminifbrozip and combin ation of these two therapies.These p atientswere divided into three grou ps and the follow-up observation was 3months.Result Curative effect of the ultrasound gr oup was better than that of drug group(P <0.05).Curative effect of the combined therapy group was the best(P <0.01).Result of follow-up suggested that serum glyceryl trioleate and cholesterol relapse rate of ultrasonic group was lower than that of drug group .Serum g lyceryl trioleate and cholesterol r elapse rate of combined therapy grou p was the lowest.Conclusion Combination of ultrasound treatmen t in vitro and administration of Gemi nifbrozip is the best way to treat hyper-lipemia,and it is not easy to relapse.展开更多
Hyperlipidemia is a risk factor for clinically significant thrombotic events in cardiovascular diseases.Platelet reactivity in hyperlipidemic conditions is enhanced when platelet scavenger receptor CD36 recognizes oxi...Hyperlipidemia is a risk factor for clinically significant thrombotic events in cardiovascular diseases.Platelet reactivity in hyperlipidemic conditions is enhanced when platelet scavenger receptor CD36 recognizes oxidized lipids in oxidized low-density lipoprotein(ox-LDL)particles,a process that induces atherothrombosis.Sulforaphane(SFN)is a dietary isothiocyanate enriched in cruciferous vegetables and exerts multiple biological activities.The current study sought to investigate the efficacy of SFN on platelet hyperreactivity under hyperlipidemic conditions in vitro and in vivo.Using a series of platelet functional assays in human platelets in vitro,we demonstrated that SFN attenuated ox-LDL-increased platelet aggregation and activation(surface CD62P expression).Mechanistically,studies using pharmacological inhibitors clarified that these inhibitory effects of SFN were mainly modulated by down-regulating CD36-mediated activation of Src kinases,leading to enhanced activation of cyclic adenosine monophosphate/protein kinase A(cAMP/PKA)signaling,and resultant inhibition of NADPH oxidase 2(NOX2)-dependent generation of reactive oxygen species(ROS).Moreover,12-week supplementation of SFN-enriched broccoli sprout extract(BSE,0.06%diet)in hyperlipidemic C57BL/6J mice also decreased platelet hyperreactivity.Studies using pharmacological inhibitors of CD36,protein kinase A(PKA)and NOX2 showed that the efficacy of BSE supplementation was mainly through modulating CD36-mediated the cAMP/PKA/NOX2 signaling.Thus,through modulating the cAMP/PKA/NOX2 pathway and attenuating CD36-mediated platelet hyperreactivity,SFN may play important protective roles in atherothrombosis under hyperlipidemic conditions.展开更多
目的系统评价我国居民糖尿病、高血压、高血脂共病患病率。方法计算机检索PubMed、Embase、the Cochrane Library、Web of Science、万方、中国知网、维普、中国生物医学文献数据库(CBM)中关于中国居民糖尿病、高血压、高血脂共病患病...目的系统评价我国居民糖尿病、高血压、高血脂共病患病率。方法计算机检索PubMed、Embase、the Cochrane Library、Web of Science、万方、中国知网、维普、中国生物医学文献数据库(CBM)中关于中国居民糖尿病、高血压、高血脂共病患病率的相关文献,并追溯纳入文献的参考文献。由两名研究人员分别进行文献筛选、资料提取和质量评价,使用Stata 16.0软件合并效应量并进行亚组分析。结果共纳入19篇文献,包含1182301名中国居民。Meta分析结果显示,我国居民糖尿病、高血压、高血脂共病患病率为5.8%(95%CI:5.1%~6.6%)。亚组分析结果显示,调查时间在2019年以前的糖尿病、高血压、高血脂共病患病率为6.3%(95%CI:4.3%~8.2%),2019年及以后为6.5%(95%CI:5.2%~7.8%);南方为5.9%(95%CI:4.7%~7.1%),北方为6.2%(95%CI:4.0%~8.3%);男性为6.3%(95%CI:5.4%~7.3%),女性为5.8%(95%CI:4.8%~6.8%);年龄<60岁为3.5%(95%CI:2.4%~4.6%),年龄≥60岁为7.9%(95%CI:4.7%~11.1%);婚姻状态在婚为5.5%(95%CI:4.4%~6.5%),未在婚为6.1%(95%CI:4.0%~8.2%);文化程度初中及以下为7.1%(95%CI:6.6%~7.6%),高中及以上为5.4%(95%CI:4.4%~6.3%);吸烟者为6.3%(95%CI:5.1%~7.5%),不吸烟者为5.8%(95%CI:4.8%~6.8%);饮酒者为7.1%(95%CI:6.4%~7.8%),不饮酒者为6.4%(95%CI:5.3%~7.6%);体重指数分类偏瘦为1.7%(95%CI:0.9%~2.4%),正常为3.8%(95%CI:2.8%~4.9%),超重为7.2%(95%CI:5.9%~8.5%),肥胖为11.0%(95%CI:8.5%~13.5%)。结论我国高血压、糖尿病、高血脂共病防控应该针对高风险人群加强健康教育,并采取有效的干预措施。展开更多
基金supported by Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ11-061,ZZ14-YQ-007)the National Natural Science Foundation of China(82004193)+1 种基金CACMS Innovation Fund(CI 2021A00914)Irma and Paul Milstein Program for Senior Health of Milstein Medical Asian American Partnership Foundation。
文摘Atherosclerosis remains a great threat to human health worldwide.Previous studies found that tetramethylpyrazine(TMP)and paeonifl orin(PF)combination(TMP-PF)exerts anti-atherosclerotic effects in vitro.However,whether TMP-PF improves atherosclerosis in vivo needs further exploration.The present study aims to assess the anti-atherosclerotic properties of TMP-PF in ApoE^(-/-)mice and explore the related molecule mechanisms.Results showed that TMP and high-dose TMP-PF decreased serum triglyceride and low-density lipoprotein cholesterol levels,suppressed vascular endothelial growth factor receptor 2(VEGFR2)and nuclear receptor subfamily 4 group A member 1(NR4A1)expression in aortic tissues,inhibited plaque angiogenesis,reduced plaque areas,and alleviated atherosclerosis in ApoE^(-/-)mice.Also,TMP-PF exhibited a better modulation effect than TMP or PF alone.However,NR4A1 agonist abolished the anti-atherosclerotic effects of TMP-PF.In conclusion,TMP-PF was first found to alleviate atherosclerosis progression by reducing hyperlipemia and inhibiting plaque angiogenesis via the NR4A1/VEGFR2 pathway,indicating that TMP-PF had a positive effect on reducing hyperlipemia and attenuating atherosclerosis development.
基金Supported by The Hebei Province TCM Administra on Bureau Founda on for Tackling Key Problems of Science and Technology: 2009058
文摘Objective To observe therapeutic effects of acupuncture on the mouse of hyperlipemia and to explore the mechanisms. Methods One hundred and twenty Kunming mice, male, sanitary degree, were randomly divided into normal group （n=40）, a model group （n=40）, an acupuncture group （n=20） and a medicine group （n=20）. Except the normal group, the mice were fed with high fat forage to prepare mouse hyperlipemia model. On the 15th day of modeling, serum total cholesterol （TC）, triglyceride （TG）, low density lipoprotein-cholesterol （LDL-C）, high density lipoprotein-cholesterol （HDL-C） levels were detected in 20 normal mice and 20 model mice; electroacupuncture was given at bilateral ＂Fēnglóng＂ （丰隆 ST 40）, ＂Qūchí＂ （曲池 LI 11）, ＂Sānyīnjiāo＂ （三阴交 SP 6） in the acupuncture group, once a day, for 10 consecutive days; in the medicine group, the mice were intragastrically administrated with Simvastatin, once daily , for 10 consecutive days. After the end of treatment, serum TC, TG, LDL-C, HDL-C, malondialdehyde （MDA）, nitric oxide （NO） and endothelin （ET） contents and superoxide dismutase （SOD） activities were detected in the groups. Results On the 15th day of modeling, in the model group serum TG, TC, LDL-C contents were significantly higher （all P〈0.05） and HDL-C content was significantly lower （P〈0.01） than those in the normal group. After treatment for 10 days, in the acupuncture group and the medicine group serum TG, TC, LDL-C, MDA and ET contents were significantly lower and serum HDL-C and NO contents and SOD activities were significantly higher than those in the model group （P〈0.05, P〈0.01）, and the improving action in the acupuncture group was better than that in the medicine group （P〈0.05, P〈0.01）. Conclusion Acupuncture can regulate fat metabolism, resist lipid peroxidation and protect vascular endothelial function in the mouse of hyperlipemia.
基金Supported by National Natural Science Fund Project of China:30873309
文摘Objective To explore the mechanism of electroacupuncture（EA） at ＂Fēnglóng＂（丰隆 ST 40） for hyperlipemia（HLP） in rats.Methods Forty health SD rats were randomly divided into a normal control group（group A）,a high fat forage group（group B） and a high fat forage ＋ treatment group（group C）,a high fat forage ＋ normal forage group（group D） and a high fat forage ＋ normal forage ＋ treatment group（group E）,eight rats in each group.EA was applied at ＂Fēnglóng＂（丰隆 ST 40） of the rats in group C and group E,once daily.After treatment of 30 days,plasma lipid levels in the rats,including total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C） were detected.Real-time quantitative polymerase chain reaction（RT-PCR） and Western Blotting were applied to detect the gene expression changes of ATP-binding cassette transporter A1（ABCA1）,peroxisome proliferator-activated receptor alpha（PPARα）,liver X receptor alpha（LXR-α） and retinoid X receptor alpha（RXR-α） in liver tissue of rats.Results Compared with group A,contents of TC,LDL-C were significantly elevated in group B and group D（all P〈0.01）;compared with group B,above indices were significantly decreased in group D（all P〈0.01）.After the treatment of EA at ＂Fēnglóng＂（丰隆 ST 40）,contents of TC,LDL-C were significantly decreased（all P〈0.01）,and contents of TG,HDL-C did not change significantly（all P〉0.05）.Compared with group A,mRNA and protein contents of ABCA1,PPARα,LXR-α and RXR-α were decreased significantly in group B and group D（all P〈0.01）.But compared with group B,the above indices were decreased in group D.After the treatment of EA,mRNA and protein contents of ABCA1,PPARα,RXR-α and LXR-α significantly increased（all P〈0.05）.Conclusion EA at ＂Fēnglóng＂（丰隆 ST 40） can decrease contents of blood TC,LDL-C in rats of hyperlipemia and up-regulate the gene expression of ABCA1,PPARα,LXR-α and RXR-α in liver,so as to promote reverse cholesterol transport,with a certain therapeutic effect on hyperlipemia.
文摘In the present paper, the effect of point-injection of Radix Salviae Miltiorrhizae injectio on blood-lipid was observed in 65 cases of hyperlipemia. The 65 patients were divided into point-injection group (40 cases who were treated with point-injection of Radix Salviae Miltiorrhizae injectio and oral administration of Shujiangzhi) and control group (25 cases who were treated with oral administration of Shujiangzhi only). Results showed that the total effective rate of the point-injection group was significantly higher than that of the control group (P<0. 05); point-injection of Radix Salviae Miltiorrhizae injectio could effectively raise the level of HDL-C, decrease lower LDL-C and regulate estrogen level of women. The slow releasing action of red sage root solution in the topical acupoint region prolonged and enhanced its effect in lowering blood-lipid. There was a significant difference between the two groups in the effect of lowering blood-fat (P< 0. 01).
文摘In this experiment, the contents of serum total cholesterol (TC), triglyceride(TG),low-density lipoprotein(LDL), high-density lipoprotein(HDL) were used as indexes to observe the effects of warming-reinforcing method on rabbit’s lipide, liopoprotein. The results indicated that in the warming-reinforcing group, TC decreased markedly and HDL increased obviously with significant difference as compared with the twirling-reinforcing group:TC and LDL also decreased, but no significant differencte as compared with the twirling-reinforcing group. Warming-reinforcing method can decrease lipids more effectively than twirling-reinforcing method.
文摘BACKGROUND Severe hyperlipemia(SHLE)has an impact on the results of many kinds of laboratory tests.Complete blood count(CBC)examination by automated blood cell counter(ABCC)is a quick and convenient measurement for screening abnormalities of blood cells that are triggered by various pathogenic insults in disease diagnosis and for monitoring changes in the treatment of existing hematological conditions.However,CBC results are frequently affected by many intrinsic and extrinsic factors from blood samples,such as in the setting of hypergammaglobulinemia and certain anticoagulants.SHLE could also affect CBC results.CASE SUMMARY A 33-year-old Chinese male presented with painful foot numbness and abdominal pain.He was initially misdiagnosed as having a myeloproliferative neoplasm(MPN)because of the marked abnormalities in CBC examination by the ABCC.Morphological evaluation of the bone marrow smears and biopsy showed no evidence of MPN.Gene mutations in Breakpoint cluster regions-Abelson murine leukemia viral oncogene homologue 1(BCR-ABL1),Janus kinase 2(JAK2),calreticulin(CALR),myeloproliferative leukemia virus(MPL),and colony-stimulating factor 3 receptor(CSF3R)were negative.Having noticed the thick chylomicron layer on blood samples and the dramatically fluctuating CBC results,we speculated that the fat droplets formed by shaking the blood samples in the setting of SHLE were mistakenly identified as blood cells due to the limited parameters of ABCC.Therefore,we removed a large part of the chylomicron layer and then reexamined the CBC,and the CBC results,as we expected,differed significantly from that of the sample before the chylomicron layer was removed.These significant differences had been validated by the subsequently repeated laboratory tests by measuring dual blood samples that the chylomicron layer was removed in one sample and was not in another,and comparing the CBC results.Computerized tomography reexamination of the upper abdomen revealed an exudative lesion surrounding his pancreas.After intensive consultation,definitive diagnosis was made as recurrent pancreatitis,hyperlipemia and pseudoerythrocytosis.CONCLUSION SHLE may become a potential cause of misdiagnosis of hyperlipemia-related diseases as MPNs and the resultant mistreatment.It may also lead to the misinterpretation of transfusion indications in patients with hematological disorders who critically need blood transfusion for supportive treatment.
文摘Hyperlipemia is a complication commonly seen in non-insulin-dependent diabetes(NIDDM).This complication can causeischemic heart disease,cerebro-vascular disease and even lifethreatening.In order to prevent this complication and to reduce mortality of NIDDM,60patients with NIDDMand hyperlipemia were investigated.Alimentary therapy consisting of the following items was given:(1)routine control of total calorie consumption:(2)carbohydrates,lipids,proteins,vitamins and minerals taken in proportional amounts;(3)fish proteins were the main proteins in the diet;(4)dried mushroom(3g/day);(5)little tea after meal;(6)corn meal or millet gruel(5-10g./day).With this dietary regime,majority of the NIDDMpatiens with serum total lipid returned to normal or near normal.
文摘Objective： To observe the therapeutic effect of antihyperlipidemic decoction on hyperlipemia. Methods： 77 hyperlipemia patients are selected and randomly divided into two groups. 37 cases in the treatment group received the treatment of antihyperlipidemic decoction, and 40 cases in the control group received western medicine, zhinbiticose. The course of treatment for both groups was 8 weeks. Results： The difference in total effective rate between the two groups was significant （P〈0.05）. Conclusion： Antihyperlipidemic decoction has a better therapeutic effect for hvoerlioemia, with a lower recurrence rate and less side effect.
文摘Objective: To observe the effect of herb-medicine-cake-separated moxibustion on serum lipoprotein in hyperlipemia rabbits. Methods: 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14), direct moxibustion group (n=14) and herb-medicine-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% lard, 0.5% cholesterol and common forage) for 6 weeks. Moxibustion was applied to “Juque”(CV 14), “Tianshu”(ST 25), “Fenglong”(ST 40), etc., 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum high density lipoprotein-cholesterol (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch) and total cholesterol (TCh) contents were assayed with colorimetric method. Results: Compared with control group, serum LDL-Ch content, HDL-Ch/LDL-Ch and HDL-Ch/TCh of model group were significantly higher (P<0.05～0.01), while compared with model group, LDL-Ch contents of two moxibustion groups were strikingly lower (P<0.01). No significant differences were found between two moxibustion groups in all the 4 indexes. Conclusion: Both direct and indirect moxibustion can effectively lower serum LDL-Ch, raise HDL-Ch, HDL-Ch/LDL-Ch and HDL-Ch/TCh, and regulate lipoprotein metabolism in hyperlipemia rabbits.
文摘Objective: To study the effect of medicinal herb-cake-separated moxibustion on serum lipoprotein levels in hyperlipemia rabbits. Methods: A total of 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14), direct moxibustion group (n=14) and medicinal herb-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% lard, 0.5% cholesterol and common forage) for 6 weeks. Moxibustion was applied to “Juque”(CV 14), “Tianshu”(ST 25), “Fenglong”(ST 40), etc., 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum triglyceride (TG) and total cholesterol (TCh) contents were assayed with colorimentric method. Results: Compared with control group, TCh and TG levels of model group increased significantly (P< 0.01). TCh and TG contents of direct moxibustion and indirect moxibustion groups were significantly lower than those of model group (P<0.01). Comparison between two moxibustion groups showed that serum TCh level of indirect moxibustion group was strikingly lower than that of direct moxibustion group (P<0.01). It indicated that both direct and indirect moxibustion could effectively lower hyperlipemia and the therapeutic effect of indirect moxibustion was significantly superior to that of direct moxibustion in lowering serum TCh level. Conclusion: Both direct and indirect moxibustion can regulate lipid metabolism and the therapeutic effect of medicinal herb-cake-separated moxibustion is superior to that of direction moxibustion in hyperlipemia rabbits.
文摘Objective: To observe effects of herbal cake-separated moxibustion on blood lipids, including total cholesterol (TCh), triglyceride (TG), high density lipoprotein-Ch (HDL-Ch), low density lipoprotein-Ch (LDL-Ch), apolipoprotein A (Apo A), apolipoprotein B (Apo B), and plasma thromboxane B2 (TXB2) and 6-keto-prostaglandin F 1α (6-keto-PGF 1α) contents and analyse its mechanism. Methods: The hyperlipemia rabbit model was established by breeding of high fat forage and injection of bovine serum albumin. They were treated respectively by direct moxibustion and herbal cake-separated moxibustion at Juque (巨阙 CV 14), Tianshu (天枢 ST 25), Fenglong (丰隆 ST 40), etc., once daily, continuously for 40 days. Blood TCh and TG contents were detected with enzymatic method, LDL-Ch and HDL-Ch with colorimetric method, Apo A and Apo B with electrophoretic method, and TXB2 and 6-keto-PGF 1α with radioimmunoassay. Results: Both the herbal cake-separated moxibustion and direct moxibustion could effectively decrease TCh, TG, LDL-Ch, Apo B and TXB2 contents and TXB2/6-keto-PGF 1α, and increase HDL-CH and 6-keto-PGF 1α contents in the rabbit of hyperlipemia. Conclusion: 6-keto-PGF 1α and TXB2 are possibly involved in the mechanism of herbal cake-separated moxibustion decreasing blood lipids.
文摘Objective To observe the effects of therapeuti c ultrasound in vitro on hyperlipemi a.Methods Comparing clinical effects of only ultrasound treatment,only adminis tration of Geminifbrozip and combin ation of these two therapies.These p atientswere divided into three grou ps and the follow-up observation was 3months.Result Curative effect of the ultrasound gr oup was better than that of drug group(P <0.05).Curative effect of the combined therapy group was the best(P <0.01).Result of follow-up suggested that serum glyceryl trioleate and cholesterol relapse rate of ultrasonic group was lower than that of drug group .Serum g lyceryl trioleate and cholesterol r elapse rate of combined therapy grou p was the lowest.Conclusion Combination of ultrasound treatmen t in vitro and administration of Gemi nifbrozip is the best way to treat hyper-lipemia,and it is not easy to relapse.
基金supported by the National Natural Science Foundation of China(82003451 and 82003455)Yunnan Fundamental Research Projects(202101AT070033)the Start-Up Fund for Introduction of High-level Talents to Dali University(YBS2021015).
文摘Hyperlipidemia is a risk factor for clinically significant thrombotic events in cardiovascular diseases.Platelet reactivity in hyperlipidemic conditions is enhanced when platelet scavenger receptor CD36 recognizes oxidized lipids in oxidized low-density lipoprotein(ox-LDL)particles,a process that induces atherothrombosis.Sulforaphane(SFN)is a dietary isothiocyanate enriched in cruciferous vegetables and exerts multiple biological activities.The current study sought to investigate the efficacy of SFN on platelet hyperreactivity under hyperlipidemic conditions in vitro and in vivo.Using a series of platelet functional assays in human platelets in vitro,we demonstrated that SFN attenuated ox-LDL-increased platelet aggregation and activation(surface CD62P expression).Mechanistically,studies using pharmacological inhibitors clarified that these inhibitory effects of SFN were mainly modulated by down-regulating CD36-mediated activation of Src kinases,leading to enhanced activation of cyclic adenosine monophosphate/protein kinase A(cAMP/PKA)signaling,and resultant inhibition of NADPH oxidase 2(NOX2)-dependent generation of reactive oxygen species(ROS).Moreover,12-week supplementation of SFN-enriched broccoli sprout extract(BSE,0.06%diet)in hyperlipidemic C57BL/6J mice also decreased platelet hyperreactivity.Studies using pharmacological inhibitors of CD36,protein kinase A(PKA)and NOX2 showed that the efficacy of BSE supplementation was mainly through modulating CD36-mediated the cAMP/PKA/NOX2 signaling.Thus,through modulating the cAMP/PKA/NOX2 pathway and attenuating CD36-mediated platelet hyperreactivity,SFN may play important protective roles in atherothrombosis under hyperlipidemic conditions.
