Objective:To investigate the bacterial infections in repeat breeder dairy cows and to evaluate the treatment effects of hyperimmune serum against two main endometritis bacteria,Escherichia(E.)coli and Trueperella pyog...Objective:To investigate the bacterial infections in repeat breeder dairy cows and to evaluate the treatment effects of hyperimmune serum against two main endometritis bacteria,Escherichia(E.)coli and Trueperella pyogenes.Methods:A total of 29 Holstein multiparous cows with three or more unsuccessful artificial inseminations were carried out and examined to confirm the absence of reproductive tract abnormality and vaginal discharges.Uterine lavage was performed to collect uterine samples for bacterial and fungal cultures.In addition,polymerase chain reaction(PCR)was done to detect Trueperella pyogenes,E.coli,Fusobacterium necrophorum,and Prevotella melaninogenicus.The cytological study was done on cervical mucus.The hyperimmune serums produced against Trueperella pyogenes and E.coli were infused into the uterus of repeat breeder cows and two doses of prostaglandin F2αwere administrated intramuscularly within 14 days'interval.Results:There were 10 positive samples in the bacterial culture with 19 isolations and no growth of the main causative bacteria of endometritis.In the PCR method,no Trueperella pyogenes,Fusobacterium necrophorum,and Prevotella melaninogenicus were found.However,11 positive samples of E.coli were identified by PCR.Cladosporium was detected in one case of repeat breeder cows.The median(interquartile range)of neutrophils in vaginal discharge was 12(22.5).Eighteen from twenty-nine(62.1%)repeat breeder and eight from eleven(72%)cows with E.coli infections in PCR were made pregnant in the first artificial insemination after treatment by intrauterine infusion of hyperimmune serum and prostaglandin F2α.Conclusions:Intrauterine administration of hyperimmune serum could be an alternative to antibiotics for the treatment of repeat breeder cows.展开更多
Immunoprophylaxis is routinely recommended for infants born to mothers with hepatitis B virus(HBV)infection within the first 12-24 hours.Detection of he-patitis B surface antibody(HBsAb)resulting from hepatitis B immu...Immunoprophylaxis is routinely recommended for infants born to mothers with hepatitis B virus(HBV)infection within the first 12-24 hours.Detection of he-patitis B surface antibody(HBsAb)resulting from hepatitis B immunoglobulin administered at birth may be perceived as a real vaccine response.This makes it difficult to detect HBV infection.For this reason,it is recommended that infants born to hepatitis B surface antigen positive mothers and who received immunop-rophylaxis at birth should have HBsAb testing when they are 9-15 months old.展开更多
A 38-year-old female presenting with a high fever of 39 ℃ developed severe liver dysfunction and acute renal failure(ARF).In tests for a hepatitis associated virus,an Immunoglobulin M-anti-hepatitis B virus core anti...A 38-year-old female presenting with a high fever of 39 ℃ developed severe liver dysfunction and acute renal failure(ARF).In tests for a hepatitis associated virus,an Immunoglobulin M-anti-hepatitis B virus core antibody was the only positive finding.Moreover,the progression of ARF coincided with the pole period of liver damage and all the other assumed causes for the ARF were unlikely.Therefore,this case was diagnosed as ARF caused by acute hepatitis B.ARF associated with non-fulminant hepatitis has been infrequently reported,usually in association with acute hepatitis A.This case is considered to be an extremely rare and interesting case.展开更多
To improve the long-term therapeutic efficacy of colorectal cancer,we propose a synergistic treatment strategy involving dualpathway,multistep induction of long-term hyperimmunity combined with photothermal-chemothera...To improve the long-term therapeutic efficacy of colorectal cancer,we propose a synergistic treatment strategy involving dualpathway,multistep induction of long-term hyperimmunity combined with photothermal-chemotherapy.To implement this strategy,infinite coordination polymer nanoparticles(SN38-Mn(II)-EGCG ICP NPs)were prepared by coordinating SN38,EGCG,and Mn2+.These nanoparticles were then coated with polydopamine(PDA)and grafted with folate-PEG-thiol(FA-PEG-SH)onto their surfaces,producing tumor-targeting folate-modified PDA infinite coordination polymer nanocomposites(ICP@FAPDA nanocomposites).These nanocomposites exhibit a particle size of 94.9±1.6 nm with a high drug loading capacity(83.3%±1.5%),drug release under acidic conditions while maintaining stability in physiological environments.Furthermore,each component within the nanocomposites serves multiple functions.Notably,the incorporation of multiple components triggers a powerful antitumor immune effect and establishes enduring immune memory through a dual-pathway and multistep approach,which is produced with the activation of the cGAS-STING pathway and immunogenic cell death(ICD)by a four-component multistep process.Under a low-dose regimen,this approach induces dual-pathway hyperimmunity effect and generates ultra-long immunological memory,marked by a ninefold increase in CD8+T cell infiltration,a fourfold increase in CD4+T lymphocytes,a fourfold reduction in Treg cells,and a fivefold increase in memory T cells.The remarkable therapy efficacy is achieved by hyperimmunity effect combination of SN38 and EGCG chemotherapy and photothermal therapy.In vivo studies demonstrated that mice treated with ICP@FA-PDA nanocomposites achieved complete eradication of cancer within 21 days,with no recurrence observed within 60 days.These nanocomposites hold significant promise and potential for future clinical translation.展开更多
文摘Objective:To investigate the bacterial infections in repeat breeder dairy cows and to evaluate the treatment effects of hyperimmune serum against two main endometritis bacteria,Escherichia(E.)coli and Trueperella pyogenes.Methods:A total of 29 Holstein multiparous cows with three or more unsuccessful artificial inseminations were carried out and examined to confirm the absence of reproductive tract abnormality and vaginal discharges.Uterine lavage was performed to collect uterine samples for bacterial and fungal cultures.In addition,polymerase chain reaction(PCR)was done to detect Trueperella pyogenes,E.coli,Fusobacterium necrophorum,and Prevotella melaninogenicus.The cytological study was done on cervical mucus.The hyperimmune serums produced against Trueperella pyogenes and E.coli were infused into the uterus of repeat breeder cows and two doses of prostaglandin F2αwere administrated intramuscularly within 14 days'interval.Results:There were 10 positive samples in the bacterial culture with 19 isolations and no growth of the main causative bacteria of endometritis.In the PCR method,no Trueperella pyogenes,Fusobacterium necrophorum,and Prevotella melaninogenicus were found.However,11 positive samples of E.coli were identified by PCR.Cladosporium was detected in one case of repeat breeder cows.The median(interquartile range)of neutrophils in vaginal discharge was 12(22.5).Eighteen from twenty-nine(62.1%)repeat breeder and eight from eleven(72%)cows with E.coli infections in PCR were made pregnant in the first artificial insemination after treatment by intrauterine infusion of hyperimmune serum and prostaglandin F2α.Conclusions:Intrauterine administration of hyperimmune serum could be an alternative to antibiotics for the treatment of repeat breeder cows.
文摘Immunoprophylaxis is routinely recommended for infants born to mothers with hepatitis B virus(HBV)infection within the first 12-24 hours.Detection of he-patitis B surface antibody(HBsAb)resulting from hepatitis B immunoglobulin administered at birth may be perceived as a real vaccine response.This makes it difficult to detect HBV infection.For this reason,it is recommended that infants born to hepatitis B surface antigen positive mothers and who received immunop-rophylaxis at birth should have HBsAb testing when they are 9-15 months old.
文摘A 38-year-old female presenting with a high fever of 39 ℃ developed severe liver dysfunction and acute renal failure(ARF).In tests for a hepatitis associated virus,an Immunoglobulin M-anti-hepatitis B virus core antibody was the only positive finding.Moreover,the progression of ARF coincided with the pole period of liver damage and all the other assumed causes for the ARF were unlikely.Therefore,this case was diagnosed as ARF caused by acute hepatitis B.ARF associated with non-fulminant hepatitis has been infrequently reported,usually in association with acute hepatitis A.This case is considered to be an extremely rare and interesting case.
基金supported by National Natural Science Foundation of China(82202308,81870380,and 82173394)China National Postdoctoral Program for Innovative Talents(BX20220247)+2 种基金China Postdoctoral Science Foundation(2021M702619)Natural Science Basic Research Plan in Shaanxi Province of China(2021TD-41 and 2020KWZ-020)Institutional Foundation of The First Affiliated Hospital of Xi’an Jiaotong University(2021QN-12).
文摘To improve the long-term therapeutic efficacy of colorectal cancer,we propose a synergistic treatment strategy involving dualpathway,multistep induction of long-term hyperimmunity combined with photothermal-chemotherapy.To implement this strategy,infinite coordination polymer nanoparticles(SN38-Mn(II)-EGCG ICP NPs)were prepared by coordinating SN38,EGCG,and Mn2+.These nanoparticles were then coated with polydopamine(PDA)and grafted with folate-PEG-thiol(FA-PEG-SH)onto their surfaces,producing tumor-targeting folate-modified PDA infinite coordination polymer nanocomposites(ICP@FAPDA nanocomposites).These nanocomposites exhibit a particle size of 94.9±1.6 nm with a high drug loading capacity(83.3%±1.5%),drug release under acidic conditions while maintaining stability in physiological environments.Furthermore,each component within the nanocomposites serves multiple functions.Notably,the incorporation of multiple components triggers a powerful antitumor immune effect and establishes enduring immune memory through a dual-pathway and multistep approach,which is produced with the activation of the cGAS-STING pathway and immunogenic cell death(ICD)by a four-component multistep process.Under a low-dose regimen,this approach induces dual-pathway hyperimmunity effect and generates ultra-long immunological memory,marked by a ninefold increase in CD8+T cell infiltration,a fourfold increase in CD4+T lymphocytes,a fourfold reduction in Treg cells,and a fivefold increase in memory T cells.The remarkable therapy efficacy is achieved by hyperimmunity effect combination of SN38 and EGCG chemotherapy and photothermal therapy.In vivo studies demonstrated that mice treated with ICP@FA-PDA nanocomposites achieved complete eradication of cancer within 21 days,with no recurrence observed within 60 days.These nanocomposites hold significant promise and potential for future clinical translation.
