Objective Molecular biological characterization of Borrelia burgdorferi DnaK gene and its epitope may be helpful to better understand the immune response that the gene caused and the interaction between the host and D...Objective Molecular biological characterization of Borrelia burgdorferi DnaK gene and its epitope may be helpful to better understand the immune response that the gene caused and the interaction between the host and DnaK gene.Methods DnaK gene of 68 representative strains including 4 subtypes of Borrelia burgdorferi found in China were sequenced using Illumina next-generation DNA sequencing method,and the sequences were compared with that sequence of the Borrelia burgdorferi sensu stricto(B.B.s.s)B31 strain.Results Comparative analyses showed that human T cell epitopes of DnaK gene had little sequence variation which led to no AA(amino acid)change and a lower overall ratio(0.03)of nonsynonymous to synonymous changes.The whole sequence variation of DnaK in Chinese main subtype B.garinii strains were more complicated.Conclusion These findings implied that hyperconserved T cell epitope in DnaK might trigger the immune system and lead to an inflammatory disease after infection.Furthermore,on the premise that protein function remains,gene variety might be related to the survival adaptation of the spirochete to the environment.展开更多
基金Major Project of The Thirteenth Five Year Special for Infectious Diseases(2016ZX10004001-004)
文摘Objective Molecular biological characterization of Borrelia burgdorferi DnaK gene and its epitope may be helpful to better understand the immune response that the gene caused and the interaction between the host and DnaK gene.Methods DnaK gene of 68 representative strains including 4 subtypes of Borrelia burgdorferi found in China were sequenced using Illumina next-generation DNA sequencing method,and the sequences were compared with that sequence of the Borrelia burgdorferi sensu stricto(B.B.s.s)B31 strain.Results Comparative analyses showed that human T cell epitopes of DnaK gene had little sequence variation which led to no AA(amino acid)change and a lower overall ratio(0.03)of nonsynonymous to synonymous changes.The whole sequence variation of DnaK in Chinese main subtype B.garinii strains were more complicated.Conclusion These findings implied that hyperconserved T cell epitope in DnaK might trigger the immune system and lead to an inflammatory disease after infection.Furthermore,on the premise that protein function remains,gene variety might be related to the survival adaptation of the spirochete to the environment.
