In order to clarify the possible relationship between the changes of behavior/personality and metabolic changes of 5 hydroxytryptamine (5 HT) in Antarctic expedition members, plasma tryptophan (Trp) and urinary 5 ...In order to clarify the possible relationship between the changes of behavior/personality and metabolic changes of 5 hydroxytryptamine (5 HT) in Antarctic expedition members, plasma tryptophan (Trp) and urinary 5 hydroxy 3 indoleacetic acid (5 HIAA) were studied for 24 winter over and 19 summer over members of the 8th and 11th CHINARE respectively. Results showed that plasma Trp decreased significantly after residing 1￣3 months at Great Wall Station and did not recover on returning back to Beijing from Antarctica by two weeks travelling. Urinary 5 HIAA increased significantly after residing 6 months at Great Wall Station, and recovered on returning back to Beijing from Antarctica in the winter over members. The decrease of plasma Trp may be related to the decline of brain 5 HT which might play a role in the changes of behavior/personality . Increase of urinary 5 HIAA might reflect metabolic changes of 5 HT as a whole, but cold weather involving in the release response of platelet should be considered. Therefore, supplement of related food rich in Trp or intervention of L Trp might be valuable.展开更多
A new fluorescent reagent, 7 (8 hydroxy 3, 6 disulfonaphthylazo) 8 hydroxyquinoline 5 sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluor...A new fluorescent reagent, 7 (8 hydroxy 3, 6 disulfonaphthylazo) 8 hydroxyquinoline 5 sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluorescent complex with λ ex / λ em =356/495 nm immediately at room temperature in ammonia ammonium chloride buffer (pH 10.7). A linear relationship was obtained in the magnesium concentration range of 0 160 ng·mL -1 with the detection limit of 0.04 ng·mL -1 . The proposed method was simple, rapid and sensitive. It has been successfully applied to the determination of trace magnesium in blood serum with recoveries of 103.75% and 98.16%, respectively.展开更多
The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N hydroxy succinimidyl 3 indolylacetate(SIIA). The derivatives of the amino...The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N hydroxy succinimidyl 3 indolylacetate(SIIA). The derivatives of the amino acids were eluted by a mobile phase of methanol water containing 10 mmol/L H 3cit Na 2HPO 4 buffer of pH 3 5 and 1 2 mmol/L tetrabutylammonium bromide on an ODS column. The detection limits were pmol level or so, respectively, when a signal to noise ratio was 3. The method was applied to the determination of amino acids in tea sample with satisfactory results.展开更多
The ternary interaction of 1, 5 bis(2 hydroxy 5 chlorphenyl) 3 cyanoformazan (HCPCF) with cetyltrimethylammonium bromide (CTAB) and rare earths (RE: Yb, Dy, Er and Eu) was investigated at pH 9.84 by the microsur...The ternary interaction of 1, 5 bis(2 hydroxy 5 chlorphenyl) 3 cyanoformazan (HCPCF) with cetyltrimethylammonium bromide (CTAB) and rare earths (RE: Yb, Dy, Er and Eu) was investigated at pH 9.84 by the microsurface adsorption spectral correction technique (MSASC). The aggregation of HCPCF on CTAB obeys the Langmuir isothermal adsorption and the interaction of RE with the HCPCF CTAB aggregate was first found to accord with the monolayer binding. The effects of temperature and ionic strength of solution on the aggregations were made. The binary aggregate and the ternary complex were characterized.展开更多
Objective: The objective of the study was to explore the regulatory mechanism of the Xiaozheng Tongluo method on lipid metabolism in liver tissue based on the sterol regulatory element-binding protein-2/(SREBP2)/3-hyd...Objective: The objective of the study was to explore the regulatory mechanism of the Xiaozheng Tongluo method on lipid metabolism in liver tissue based on the sterol regulatory element-binding protein-2/(SREBP2)/3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGCR)/low-density lipoprotein receptor(LDLR) signaling pathway. Materials and Methods: C57BL/6 mice were selected as the blank group. The apolipoprotein E-deficient(Apo E^(-/-)) mice were randomly divided into the model group, traditional Chinese medicine(TCM) group, and control group. The general condition of the mice was determined by the mouse's state and liver weight. Liver hematoxylin and eosin staining and Oil Red O staining were used to observe the pathological changes and lipid droplet deposition of the liver. The protein expression levels of SREBP2, HMGCR, and LDLR were detected by Western blotting and polymerase chain reaction. Results:(1) The rats in the model group were in poor condition, and their liver weight increased significantly. Compared with the model group, the condition of the TCM group and the control group improved to varying degrees, and their liver weight decreased significantly.(2) Compared with the normal group, the hepatocytes in the model group were arranged in a disorderly manner,and the red-stained lipids of stem cells increased significantly. Compared with the model group, the degree of liver lesions in the control group and TCM group was reduced, and the red-stained lipid of hepatocytes was significantly reduced.(3) Compared with the blank group, the expression of SREBP2 and HMGCR protein in the model group increased significantly, and the expression of LDLR protein decreased significantly(P < 0.05).Compared with the model group, the expression of SREBP2 and HMGCR protein in the TCM group decreased significantly, and the expression of LDLR protein increased significantly(P < 0.05). Conclusions: The method detailed in this paper can increase the expression of SREBP2 and HMGCR protein and decrease the expression of LDLR protein, thus regulating liver cholesterol metabolism and delaying the progression of atherosclerosis.展开更多
文摘In order to clarify the possible relationship between the changes of behavior/personality and metabolic changes of 5 hydroxytryptamine (5 HT) in Antarctic expedition members, plasma tryptophan (Trp) and urinary 5 hydroxy 3 indoleacetic acid (5 HIAA) were studied for 24 winter over and 19 summer over members of the 8th and 11th CHINARE respectively. Results showed that plasma Trp decreased significantly after residing 1￣3 months at Great Wall Station and did not recover on returning back to Beijing from Antarctica by two weeks travelling. Urinary 5 HIAA increased significantly after residing 6 months at Great Wall Station, and recovered on returning back to Beijing from Antarctica in the winter over members. The decrease of plasma Trp may be related to the decline of brain 5 HT which might play a role in the changes of behavior/personality . Increase of urinary 5 HIAA might reflect metabolic changes of 5 HT as a whole, but cold weather involving in the release response of platelet should be considered. Therefore, supplement of related food rich in Trp or intervention of L Trp might be valuable.
基金Supported by the Zi-Qiang Foundation of Wuhan U niversit
文摘A new fluorescent reagent, 7 (8 hydroxy 3, 6 disulfonaphthylazo) 8 hydroxyquinoline 5 sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluorescent complex with λ ex / λ em =356/495 nm immediately at room temperature in ammonia ammonium chloride buffer (pH 10.7). A linear relationship was obtained in the magnesium concentration range of 0 160 ng·mL -1 with the detection limit of 0.04 ng·mL -1 . The proposed method was simple, rapid and sensitive. It has been successfully applied to the determination of trace magnesium in blood serum with recoveries of 103.75% and 98.16%, respectively.
文摘The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N hydroxy succinimidyl 3 indolylacetate(SIIA). The derivatives of the amino acids were eluted by a mobile phase of methanol water containing 10 mmol/L H 3cit Na 2HPO 4 buffer of pH 3 5 and 1 2 mmol/L tetrabutylammonium bromide on an ODS column. The detection limits were pmol level or so, respectively, when a signal to noise ratio was 3. The method was applied to the determination of amino acids in tea sample with satisfactory results.
基金ProjectsupportedbytheNaturalScienceFoundationofAnhuiProvince (No .0 1 0 45 30 1 )andtheAnhuiProvincialScienceandTechnologyforExcellentYouths
文摘The ternary interaction of 1, 5 bis(2 hydroxy 5 chlorphenyl) 3 cyanoformazan (HCPCF) with cetyltrimethylammonium bromide (CTAB) and rare earths (RE: Yb, Dy, Er and Eu) was investigated at pH 9.84 by the microsurface adsorption spectral correction technique (MSASC). The aggregation of HCPCF on CTAB obeys the Langmuir isothermal adsorption and the interaction of RE with the HCPCF CTAB aggregate was first found to accord with the monolayer binding. The effects of temperature and ionic strength of solution on the aggregations were made. The binary aggregate and the ternary complex were characterized.
基金supported by the Science and Technology Support Program Project of the Hebei Provincial Department of Science and Technology (No. 19277753D)the Scientific Research Project of the Hebei Administration of TCM (No. 2019004, No. 2019066)
文摘Objective: The objective of the study was to explore the regulatory mechanism of the Xiaozheng Tongluo method on lipid metabolism in liver tissue based on the sterol regulatory element-binding protein-2/(SREBP2)/3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGCR)/low-density lipoprotein receptor(LDLR) signaling pathway. Materials and Methods: C57BL/6 mice were selected as the blank group. The apolipoprotein E-deficient(Apo E^(-/-)) mice were randomly divided into the model group, traditional Chinese medicine(TCM) group, and control group. The general condition of the mice was determined by the mouse's state and liver weight. Liver hematoxylin and eosin staining and Oil Red O staining were used to observe the pathological changes and lipid droplet deposition of the liver. The protein expression levels of SREBP2, HMGCR, and LDLR were detected by Western blotting and polymerase chain reaction. Results:(1) The rats in the model group were in poor condition, and their liver weight increased significantly. Compared with the model group, the condition of the TCM group and the control group improved to varying degrees, and their liver weight decreased significantly.(2) Compared with the normal group, the hepatocytes in the model group were arranged in a disorderly manner,and the red-stained lipids of stem cells increased significantly. Compared with the model group, the degree of liver lesions in the control group and TCM group was reduced, and the red-stained lipid of hepatocytes was significantly reduced.(3) Compared with the blank group, the expression of SREBP2 and HMGCR protein in the model group increased significantly, and the expression of LDLR protein decreased significantly(P < 0.05).Compared with the model group, the expression of SREBP2 and HMGCR protein in the TCM group decreased significantly, and the expression of LDLR protein increased significantly(P < 0.05). Conclusions: The method detailed in this paper can increase the expression of SREBP2 and HMGCR protein and decrease the expression of LDLR protein, thus regulating liver cholesterol metabolism and delaying the progression of atherosclerosis.
