Bone regeneration for non-load-bearing defects remains a significant clinical challenge requiring advanced biomaterials and cellular strategies.Adiposederived mesenchymal stem cells(AD-MSCs)have garnered significant i...Bone regeneration for non-load-bearing defects remains a significant clinical challenge requiring advanced biomaterials and cellular strategies.Adiposederived mesenchymal stem cells(AD-MSCs)have garnered significant interest in bone tissue engineering(BTE)because of their abundant availability,minimally invasive harvesting procedures,and robust differentiation potential into osteogenic lineages.Unlike bone marrow-derived mesenchymal stem cells,AD-MSCs can be easily obtained in large quantities,making them appealing alternatives for therapeutic applications.This review explores hydrogels containing polymers,such as chitosan,collagen,gelatin,and hyaluronic acid,and their composites,tailored for BTE,and emphasizes the importance of these hydrogels as scaffolds for the delivery of AD-MSCs.Various hydrogel fabrication techniques and biocompatibility assessments are discussed,along with innovative modifications to enhance osteogenesis.This review also briefly outlines AD-MSC isolation methods and advanced embedding techniques for precise cell placement,such as direct encapsulation and three-dimensional bioprinting.We discuss the mechanisms of bone regeneration in the AD-MSC-laden hydrogels,including osteoinduction,vascularization,and extracellular matrix remodeling.We also review the preclinical and clinical applications of AD-MSC-hydrogel systems,emphasizing their success and limitations.In this review,we provide a comprehensive overview of AD-MSC-based hydrogel systems to guide the development of effective therapies for bone regeneration.展开更多
Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic diseases.However,conventional topical drug deliv...Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic diseases.However,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in vivo.Therefore,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic stroke.Matrix metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ischemia.Herein,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel PA-TIMP-QK.PA-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in vitro.The results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor function.These findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.展开更多
Thermo-respansive chitosan hydrogel system （TRCHS） was prepared and its mierostructure was investigated by scaning electron microscope （SEM） and mercury intrusion poremaster （MIP）. Based on analyzing the data, a...Thermo-respansive chitosan hydrogel system （TRCHS） was prepared and its mierostructure was investigated by scaning electron microscope （SEM） and mercury intrusion poremaster （MIP）. Based on analyzing the data, a special porosity property was reported at the first time. Its gelling mechanism was studied by a group of contrast experiments. Results may provide experimental and theoretical supports for how to apply it on tissue engineering scaffold and how to influeuee or control its essential properties.展开更多
In this paper the suitability of a graft polymer nanocomposite hydrogel system for enhanced oil recovery was examined using polyacrylamide graft starch/clay nanocomposite(a laboratory synthesized product) and chromium...In this paper the suitability of a graft polymer nanocomposite hydrogel system for enhanced oil recovery was examined using polyacrylamide graft starch/clay nanocomposite(a laboratory synthesized product) and chromium(III) acetate(crosslinker). X-ray diffraction analysis,Fourier transform infrared spectrometry analysis, field-emission scanning electron microscopy and transmission electron microscopy were carried out to reveal the laboratory synthesized product as a nanocomposite. The effects of various parameters like salt concentration, p H, temperature, polymer concentration and crosslinker concentration on the properties of the developed gel system were systematically evaluated.The thermal stability of the nanocomposite gel and the conventional gel system were also determined by thermogravimetric analysis. The graft polymer nanocomposite gel system exhibited acceptable gel strength, gelation time and gel stability compared with the conventional gel system. The nanocomposite gels prepared using a low crosslinker concentration showed higher gel strength and required longer gelation time than the conventional gel which is more desirable properties for the effective placement of gel during enhanced oil recovery operations. In addition, sand pack flooding experiments show that the graft polymer nanocomposite gels had better plugging capacity than the conventional gel systems under reservoir conditions. Hence, this gel system may be suitable in the water shutoff treatments required for enhanced oil recovery from oilfields.展开更多
Halloysite nanotube-composited thermo-responsive hydrogel system has been successfully developed for controlled drug release by copolymerization of N-isopropylacrylamide （NIPAM） with silane-modified halloysite nanot...Halloysite nanotube-composited thermo-responsive hydrogel system has been successfully developed for controlled drug release by copolymerization of N-isopropylacrylamide （NIPAM） with silane-modified halloysite nanotubes （HNT） through thermally initiated free-radical polymerization. With methylene blue as a model drug, thermo-responsive drug release results demonstrate that the drug release from the nanotubes in the composited hy-drogel can^be well controlled by manipulating the environmental temperature. When the hydrogel network is swol- len at temperature below the lower critical solution temperature （LCST）, drug releases steadily from lumens of the embedded nanotubes, whereas the drug release stops when hydrogel shrinks at temperature above the LCST. The release of model drug from the HNT-composited hydrogel matches well with its thermo-responsive volume phasetransition, and shows characteristics of well controlled release. The design strategy and release results of the pro- posed novel HNT-composited thermo-responsive hydrogel system provide valuable guidance for designing respon- s_i_ve nanocomposites for controlled-release of active agents.展开更多
The impaired function of periodontal ligament stem cells(PDLSCs)impedes restoration of periodontal tissues.The cGAS-cGAMP-STING pathway is an innate immune pathway that sensing cytosolic double-stranded DNA(dsDNA),but...The impaired function of periodontal ligament stem cells(PDLSCs)impedes restoration of periodontal tissues.The cGAS-cGAMP-STING pathway is an innate immune pathway that sensing cytosolic double-stranded DNA(dsDNA),but its role in regulating the function of PDLSCs is still unclear.In this study,we found that mito-chondrial DNA(mtDNA)was released into the cytoplasm through the mitochondrial permeability transition pore(mPTP)in PDLSCs upon inflammation,which binds to cGAS and activated the STING pathway by promoting the production of cGAMP,and ultimately impaired the osteogenic differentiation of PDLSCs.Additionally,it is first found that inflammation can down-regulate the level of the ATP-binding cassette membrane subfamily member C1(ABCC1,a cGAMP exocellular transporter)and ectonucleotide pyrophosphatase/phosphodiesterase 1(ENPP1,a cGAMP hydrolase),which further aggravated the accumulation of intracellular cGAMP,leading to the persistent activation of the cGAS-STING pathway and thus the impaired the differentiation capacity of PDLSCs.Furthermore,we designed a hydrogel system loaded with a mPTP blocker,an ABCC1 agonist and ENPP1 to promote periodontal tissue regeneration by modulating the production,exocytosis,and clearance of cGAMP.In conclusion,our results highlight the profound effects,and specific mechanisms,of the cGAS-STING pathway on the function of stem cells and propose a new strategy to promote periodontal tissue restoration based on the reestablishment of cGAMP homeostasis.展开更多
Smart farming with outdoor monitoring systems is critical to address food shortages and sustainability challenges.These systems facilitate informed decisions that enhance efficiency in broader environmental management...Smart farming with outdoor monitoring systems is critical to address food shortages and sustainability challenges.These systems facilitate informed decisions that enhance efficiency in broader environmental management.Existing outdoor systems equipped with energy harvesters and self-powered sensors often struggle with fluctuating energy sources,low durability under harsh conditions,non-transparent or non-biocompatible materials,and complex structures.Herein,a multifunctional hydrogel is developed,which can fulfill all the above requirements and build selfsustainable outdoor monitoring systems solely by it.It can serve as a stable energy harvester that continuously generates direct current output with an average power density of 1.9 W m^(-3)for nearly 60 days of operation in normal environments(24℃,60%RH),with an energy density of around 1.36×10^(7)J m^(-3).It also shows good self-recoverability in severe environments(45℃,30%RH)in nearly 40 days of continuous operation.Moreover,this hydrogel enables noninvasive and self-powered monitoring of leaf relative water content,providing critical data on evaluating plant health,previously obtainable only through invasive or high-power consumption methods.Its potential extends to acting as other self-powered environmental sensors.This multifunctional hydrogel enables self-sustainable outdoor systems with scalable and low-cost production,paving the way for future agriculture.展开更多
All-season thermal management with zero energy consumption and emissions is more crucial to global decarbonization over traditional energy-intensive cooling/heating systems.However,the static single thermal management...All-season thermal management with zero energy consumption and emissions is more crucial to global decarbonization over traditional energy-intensive cooling/heating systems.However,the static single thermal management for cooling or heating fails to self-regulate the temperature in dynamic seasonal temperature condition.Herein,inspired by the dual-temperature regulation function of the fur color changes on the backs and abdomens of penguins,a smart thermal management composite hydrogel(PNA@H-PM Gel)system was subtly created though an"on-demand"dual-layer structure design strategy.The PNA@H-PM Gel system features synchronous solar and thermal radiation modulation as well as tunable phase transition temperatures to meet the variable seasonal thermal requirements and energy-saving demands via self-adaptive radiative cooling and solar heating regulation.Furthermore,this system demonstrates superb modulations of both the solar reflectance(ΔR=0.74)and thermal emissivity(ΔE=0.52)in response to ambient temperature changes,highlighting efficient temperature regulation with average radiative cooling and solar heating effects of 9.6℃in summer and 6.1℃in winter,respectively.Moreover,compared to standard building baselines,the PNA@H-PM Gel presents a more substantial energy-saving cooling/heating potentials for energy-efficient buildings across various regions and climates.This novel solution,inspired by penguins in the real world,will offer a fresh approach for producing intelligent,energy-saving thermal management materials,and serve for temperature regulation under dynamic climate conditions and even throughout all seasons.展开更多
Artificial sensory systems,designed to emulate human senses like sight,touch,and hearing,have garnered significant attention for their potential to enhance human capabilities,improve human-machine interactions,and ena...Artificial sensory systems,designed to emulate human senses like sight,touch,and hearing,have garnered significant attention for their potential to enhance human capabilities,improve human-machine interactions,and enable autonomous systems to better perceive their surroundings.Hydrogels,with their biocompatibility,flexibility,and water-rich polymer structure,are increasingly recognized as crucial materials in the development of these systems,especially in applications such as wearable sensors,artificial skin,and neural interfaces.This review explores various hydrogel fabrication techniques,including 3D bioprinting,electro spinning,and photopolymerization,which allow for the precise control of hydrogel properties like mechanical strength,flexibility,and conductivity.By tailoring these properties to mimic natural tissues,hydrogels offer transformative benefits in the creation of advanced,biocompatible,and durable sensory systems.We emphasize the importance of selecting appropriate fabrication methods to meet the specific functional requirements of artificial sensory applications,such as sensitivity to stimuli,durability,and ease of integration.This review further highlights the pivotal role of hydrogels in advancing future artificial sensory technologies and their broad potential in fields ranging from robotics to biomedical devices.展开更多
Traditional cancer therapies have limitations like poor efficacy on advanced tumors,healthy tissue damage,side effects,and drug resistance,creating an urgent need for new strategies.Hydrogels have good biocompatibilit...Traditional cancer therapies have limitations like poor efficacy on advanced tumors,healthy tissue damage,side effects,and drug resistance,creating an urgent need for new strategies.Hydrogels have good biocompatibility and controlled release,while extracellular vesicles(EVs)enable targeting and bioactive transport.This review systematically summarizes hydrogels and EVs,focusing on the construction of hydrogel-EV delivery system,key influencing factors,drug delivery mechanisms,and tumor therapy apps,clarifying their synergies.The system overcomes single-carrier flaws,construction methods/key factors affect performance,preclinical studies have confirmed efficacy in multiple therapies,but large-scale production and in vivo stability challenges remain,yet it promises to overcome the limits of traditional therapy for precision oncology.展开更多
Meniscus injuries present significant therapeutic challenges due to their limited self-healing capacity and the diverse biological and mechanical properties across the tissue.Conventional repair strategies do not repl...Meniscus injuries present significant therapeutic challenges due to their limited self-healing capacity and the diverse biological and mechanical properties across the tissue.Conventional repair strategies do not replicate the complex zonal characteristics within the meniscus,resulting in suboptimal outcomes.In this study,we introduce an innovative fetal/adult and stiffness-tunable meniscus decellularized extracellular matrix(DEM)-based hydrogel system designed for precision repair of heterogeneous,zonal-dependent meniscus injuries.By syn-thesizing fetal and adult DEM hydrogels,we identified distinct cellular responses,including that hydrogels with adult meniscus-derived DEM promote more fibrochondrogenic phenotypes.The incorporation of methacrylated hyaluronic acid(MeHA)further refined the mechanical properties and injectability of the DEM-based hydrogels.The combination of fetal and adult DEM with MeHA allowed for precise tuning of stiffness,influencing cell differentiation and closely mimicking native tissue environments.In vivo tests confirmed the biocompatibility of hydrogels and their integration with native meniscus tissues.Furthermore,advanced 3D bioprinting techniques enabled the fabrication of hybrid hydrogels with biomaterial and mechanical gradients,effectively emulating the zonal properties of meniscus tissue and enhancing cell integration.This study represents a significant advance in meniscus tissue engineering,providing a promising platform for customized regenerative therapies across a range of heterogeneous fibrous connective tissues.展开更多
This study presents a thorough investigation into the use of single and twin-tailed cationic and anionic surfactant-modified chitosan(SMCS)hydrogel beads as effective adsorbents for the elimination of hazardous polycy...This study presents a thorough investigation into the use of single and twin-tailed cationic and anionic surfactant-modified chitosan(SMCS)hydrogel beads as effective adsorbents for the elimination of hazardous polycyclic aromatic hydrocarbons(PAHs)from aqueous solutions.The Chitosan(CS)hydrogel beads were modified with single/twin-tailed anionic surfactants,sodium dodecyl sulfate(SDS)and sodium bis(2-ethylhexyl)sulfosuccinate(AOT),and cationic surfactants,dodecyltrimethylammonium bromide(DTAB)and didodecyldimethylammonium bromide(DDAB),to enhance their adsorption capacity of PAHs.The CS and SMCS beads were evaluated for their structural,mechanical,and adsorption properties using a range of techniques,including infrared spectroscopy(IR),energy-dispersive X-ray spectroscopy(EDX),rheometry,and field emission scanning electron microscopy(FESEM).Adsorption experiments of naphthalene(Nap),acenaphthene(Ace),and phenanthrene(Phe)on SMCS beads demonstrate that they have significantly higher adsorption capacities than CS beads,due to increase in hydrophobic interactions.Adsorption capacity followed the trend,Phen>Ace>Nap for all the beads revealing that twin-tailed SMCS bead possess much higher adsorption capacities(Qmax)compared to single-tailed SMCS beads.For twin tailed surfactants,the maximum adsorption capacities for Nap,Ace and Phe varied as CS-AOT(CS-DDAB):430.0(323.8)611.60(538.18)633.39(536.99)mg/g respectively,outperforming other reported hydrogel beads.The study highlights the simplicity,eco-friendliness,and enhanced performance of surfactant modification for developing high-efficiency adsorbents,paving the way for cost-effective solutions in water re-mediation.展开更多
By combining the merits of radiative cooling(RC)and evaporation cooling(EC),radiative coupled evaporative cooling(REC)has attracted considerable attention for sub-ambient cooling purposes.However,for outdoor devices,t...By combining the merits of radiative cooling(RC)and evaporation cooling(EC),radiative coupled evaporative cooling(REC)has attracted considerable attention for sub-ambient cooling purposes.However,for outdoor devices,the interior heating power would increase the working temperature and fire risk,which would suppress their above-ambient heat dissipation capabilities and passive water cycle properties.In this work,we introduced a REC design based on an all-in-one photonic hydrogel for above-ambient heat dissipation and flame retardancy.Unlike conventional design RC film for heat dissipation with limited cooling power and fire risk,REC hydrogel can greatly improve the heat dissipation performance in the daytime with a high workload,indicating a 12.0℃lower temperature than the RC film under the same conditions in the outdoor experiment.In the nighttime with a low workload,RC-assisted adsorption can improve atmospheric water harvesting to ensure EC in the daytime.In addition,our REC hydrogel significantly enhanced flame retardancy by absorbing heat without a corresponding temperature rise,thus mitigating fire risks.Thus,our design shows a promising solution for the thermal management of outdoor devices,delivering outstanding performance in both heat dissipation and flame retardancy.展开更多
Solar-driven interfacial desalination(SID)offers a sustainable route for freshwater production,yet its long-term performance is compromised by salt crystallization and microbial fouling under complex marine conditions...Solar-driven interfacial desalination(SID)offers a sustainable route for freshwater production,yet its long-term performance is compromised by salt crystallization and microbial fouling under complex marine conditions.Zwitterionic polymers offer promising nonfouling capabilities,but current zwitterionic hydrogel-based solar evaporators(HSEs)suffer from inadequate hydration and salt vulnerability.Inspired by the natural marine environmental adaptive characteristics of saltwater fish,we report a superhydrated zwitterionic poly(trimethylamine N-oxide,PTMAO)/polyacrylamide(PAAm)/polypyrrole(PPy)hydrogel(PTAP)with dedicated water channels for efficient,durable,and nonfouling SID.The directly linked N⁺and O⁻groups in PTMAO establish a robust hydration shell that facilitates rapid water transport while resisting salt and microbial adhesion.Integrated PAAm and PPy networks enhance mechanical strength and photothermal conversion.PTAP achieves a high evaporation rate of 2.35 kg m^(−2)h^(−1)under 1 kW m^(–2)in 10 wt%NaCl solution,maintaining stable operation over 100 h without salt accumulation.Furthermore,PTAP effectively resists various foulants including proteins,bacterial,and algal adhesion.Molecular dynamics simulations reveal that the exceptional hydration capacity supports its nonfouling properties.This work advances the development of nonfouling HSEs for sustainable solar desalination in real-world marine environments.展开更多
Octopuses,due to their flexible arms,marvelous adaptability,and powerful suckers,are able to effortlessly grasp and disengage various objects in the marine surrounding without causing devastation.However,manipulating ...Octopuses,due to their flexible arms,marvelous adaptability,and powerful suckers,are able to effortlessly grasp and disengage various objects in the marine surrounding without causing devastation.However,manipulating delicate objects such as soft and fragile foods underwater require gentle contact and stable adhesion,which poses a serious challenge to now available soft grippers.Inspired by the sucker infundibulum structure and flexible tentacles of octopus,herein we developed a hydraulically actuated hydrogel soft gripper with adaptive maneuverability by coupling multiple hydrogen bond-mediated supramolecular hydrogels and vat polymerization three-dimensional printing,in which hydrogel bionic sucker is composed of a tunable curvature membrane,a negative pressure cavity,and a pneumatic chamber.The design of the sucker structure with the alterable curvature membrane is conducive to realize the reliable and gentle switchable adhesion of the hydrogel soft gripper.As a proof-of-concept,the adaptive hydrogel soft gripper is capable of implement diversified underwater tasks,including gingerly grasping fragile foods like egg yolks and tofu,as well as underwater robots and vehicles that station-keeping and crawling based on switchable adhesion.This study therefore provides a transformative strategy for the design of novel soft grippers that will render promising utilities for underwater exploration soft robotics.展开更多
Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological prop...Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological properties and tissue specificity,highlighting its potential as a biomedical material for the repair of severe peripheral nerve injury;however,its role in modulating neuroinflammation post-peripheral nerve injury remains unknown.Here,we aimed to characterize the anti-inflammatory properties of porcine decellularized nerve matrix hydrogel and their underlying molecular mechanisms.Using peripheral nerve injury model rats treated with porcine decellularized nerve matrix hydrogel,we evaluated structural and functional recovery,macrophage phenotype alteration,specific cytokine expression,and changes in related signaling molecules in vivo.Similar parameters were evaluated in vitro using monocyte/macrophage cell lines stimulated with lipopolysaccharide and cultured on porcine decellularized nerve matrix hydrogel-coated plates in complete medium.These comprehensive analyses revealed that porcine decellularized nerve matrix hydrogel attenuated the activation of excessive inflammation at the early stage of peripheral nerve injury and increased the proportion of the M2 subtype in monocytes/macrophages.Additionally,porcine decellularized nerve matrix hydrogel negatively regulated the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB axis both in vivo and in vitro.Our findings suggest that the efficacious anti-inflammatory properties of porcine decellularized nerve matrix hydrogel induce M2 macrophage polarization via suppression of the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB pathway,providing new insights into the therapeutic mechanism of porcine decellularized nerve matrix hydrogel in peripheral nerve injury.展开更多
The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly f...The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly formed neurons to the damaged area.However,only a small percentage of these neurons survive,and many do not reach the damaged area,possibly because the corpus callosum impedes the migration of subventricular zone-derived stem cells into the lesioned cortex.A second major obstacle to stem cell therapy is the strong inflammatory reaction induced by cerebral ischemia,whereby the associated phagocytic activity of brain macrophages removes both therapeutic cells and/or cell-based drug carriers.To address these issues,neurogenesis was electrically stimulated in the subventricular zone,followed by isolation of proliferating cells,including newly formed neurons,which were subsequently mixed with a nutritional hydrogel.This mixture was then transferred to the stroke cavity of day 14 post-stroke mice.We found that the performance of the treated animals improved in behavioral tests,including novel object,open field,hole board,grooming,and“time-to-feel”adhesive tape tests.Furthermore,immunostaining revealed that the stem cell marker nestin,the neuroepithelial marker Mash1,and the immature neuronal marker doublecortin-positive cells survived in the transplanted area for 2 weeks,possibly due to reduced phagocytic activity and supportive angiogenesis.These results clearly indicate that the transplantation of committed subventricular zone stem cells combined with a protective nutritional gel directly into the infarct cavity after the peak of stroke-induced neuroinflammation represents a feasible approach to improve neurorestoration after cerebral ischemia.展开更多
Treatment of osteoarthritis(OA)by administration of corticosteroids is a commonly used method in clinics using anti-inflammatory medicine.Oral administration or intra-articular injection of corticosteroids can reduce ...Treatment of osteoarthritis(OA)by administration of corticosteroids is a commonly used method in clinics using anti-inflammatory medicine.Oral administration or intra-articular injection of corticosteroids can reduce the pain and progress of cartilage degeneration,but they are usually insufficient to show local and long-term anti-inflammatory effects because of their fast clearance in the body.In this study,we suggest an injectable anti-OA drug depot system for sustained drug release that provides long-term effective therapeutic advantages.Amphiphilic poly(organophosphazene),which has temperature-dependent nanoparticle forming and sol-gel transition behaviors when dissolved in aqueous solution,was synthesized for triamcinolone acetonide(TCA)delivery.Because hydrophobic parts of the polymer can interact with hydrophobic parts of the TCA,the TCA was encapsulated into the self-assembled polymeric nanoparticles.The TCA-encapsulated polymeric nanoparticles(TePNs)were well dispersed in an aqueous solution below room temperature so that they can be easily injected as a sol state into an intra-articular region.However,the TePNs solution transforms immediately to a viscose 3D hydrogel like a synovial fluid in the intra-articular region via the conducted body temperature.An in vitro TCA release study showed sustained TCA release for six weeks.One-time injection of the TePN hydrogel system in an early stage of OA-induced rat model showed a great inhibition effect against further OA progression.The OA-induced knees completely recovered as a healthy cartilage without any abnormal symptoms.展开更多
Mandibular defect becomes a prevalent maxillofacial disease resulting in mandibular dysfunctions and huge psychological burdens to the patients.Considering the routine presence of oral contaminations and aesthetic res...Mandibular defect becomes a prevalent maxillofacial disease resulting in mandibular dysfunctions and huge psychological burdens to the patients.Considering the routine presence of oral contaminations and aesthetic restoration of facial structures,the current clinical treatments are however limited,incapable to reconstruct the structural integrity and regeneration,spurring the need for cost-effective mandibular tissue engineering.Hydrogel systems possess great merit for mandibular reconstruction with precise involvement of cells and bioactive factors.In this review,current clinical treatments and distinct mode(s)of mandible formation and pathological resorption are summarized,followed by a review of hydrogel-related mandibular tissue engineering,and an update on the advanced fabrication of hydrogels with improved mechanical property,antibacterial ability,injectable form,and 3D bioprinted hydrogel constructs.The exploration of advanced hydrogel systems will lay down a solid foundation for a bright future with more biocompatible,effective,and personalized treatment in mandibular reconstruction.展开更多
Although neoantigen-based cancer vaccines show great potential in cancer immunotherapy due to their ability to induce effective and long-lasting anti-tumor immunity,their development is hindered by the limitations of ...Although neoantigen-based cancer vaccines show great potential in cancer immunotherapy due to their ability to induce effective and long-lasting anti-tumor immunity,their development is hindered by the limitations of neoantigens identification,low immunogenicity,and weak immune response.Cyclophosphamide(CTX)not only directly kills tumors but also causes immunogenic cell death,providing a promising source of antigens for cancer vaccines.Herein,a combined immunotherapy strategy based on temperature-sensitive PLEL hydrogel is designed.First,CTX-loaded hydrogel is injected intratumorally into CT26 bearing mice to prime anti-tumor immunity,and then 3 days later,PLEL hydrogels loaded with CpG and tumor lysates are subcutaneously injected into both groins to further promote anti-tumor immune responses.The results confirm that this combined strategy reduces the toxicity of CTX,and produces the cytotoxic T lymphocyte response to effectively inhibit tumor growth,prolong survival,and significantly improve the tumor cure rate.Moreover,a long-lasting immune memory response is observed in the mice.About 90%of the cured mice survive for at least 60 days after being re-inoculated with tumors,and the distant tumor growth is also well inhibited.Hence,this PLEL-based combination therapy may provide a promising reference for the clinical promotion of chemotherapy combined with cancer vaccines.展开更多
文摘Bone regeneration for non-load-bearing defects remains a significant clinical challenge requiring advanced biomaterials and cellular strategies.Adiposederived mesenchymal stem cells(AD-MSCs)have garnered significant interest in bone tissue engineering(BTE)because of their abundant availability,minimally invasive harvesting procedures,and robust differentiation potential into osteogenic lineages.Unlike bone marrow-derived mesenchymal stem cells,AD-MSCs can be easily obtained in large quantities,making them appealing alternatives for therapeutic applications.This review explores hydrogels containing polymers,such as chitosan,collagen,gelatin,and hyaluronic acid,and their composites,tailored for BTE,and emphasizes the importance of these hydrogels as scaffolds for the delivery of AD-MSCs.Various hydrogel fabrication techniques and biocompatibility assessments are discussed,along with innovative modifications to enhance osteogenesis.This review also briefly outlines AD-MSC isolation methods and advanced embedding techniques for precise cell placement,such as direct encapsulation and three-dimensional bioprinting.We discuss the mechanisms of bone regeneration in the AD-MSC-laden hydrogels,including osteoinduction,vascularization,and extracellular matrix remodeling.We also review the preclinical and clinical applications of AD-MSC-hydrogel systems,emphasizing their success and limitations.In this review,we provide a comprehensive overview of AD-MSC-based hydrogel systems to guide the development of effective therapies for bone regeneration.
基金supported by the Natural Science Foundation of Shandong Province,No.ZR2023MC168the National Natural Science Foundation of China,No.31670989the Key R&D Program of Shandong Province,No.2019GSF107037(all to CS).
文摘Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic diseases.However,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in vivo.Therefore,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic stroke.Matrix metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ischemia.Herein,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel PA-TIMP-QK.PA-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in vitro.The results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor function.These findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.
文摘Thermo-respansive chitosan hydrogel system （TRCHS） was prepared and its mierostructure was investigated by scaning electron microscope （SEM） and mercury intrusion poremaster （MIP）. Based on analyzing the data, a special porosity property was reported at the first time. Its gelling mechanism was studied by a group of contrast experiments. Results may provide experimental and theoretical supports for how to apply it on tissue engineering scaffold and how to influeuee or control its essential properties.
基金the Indian Institute of Technology(Indian School of Mines),Dhanbad,India,for providing financial support
文摘In this paper the suitability of a graft polymer nanocomposite hydrogel system for enhanced oil recovery was examined using polyacrylamide graft starch/clay nanocomposite(a laboratory synthesized product) and chromium(III) acetate(crosslinker). X-ray diffraction analysis,Fourier transform infrared spectrometry analysis, field-emission scanning electron microscopy and transmission electron microscopy were carried out to reveal the laboratory synthesized product as a nanocomposite. The effects of various parameters like salt concentration, p H, temperature, polymer concentration and crosslinker concentration on the properties of the developed gel system were systematically evaluated.The thermal stability of the nanocomposite gel and the conventional gel system were also determined by thermogravimetric analysis. The graft polymer nanocomposite gel system exhibited acceptable gel strength, gelation time and gel stability compared with the conventional gel system. The nanocomposite gels prepared using a low crosslinker concentration showed higher gel strength and required longer gelation time than the conventional gel which is more desirable properties for the effective placement of gel during enhanced oil recovery operations. In addition, sand pack flooding experiments show that the graft polymer nanocomposite gels had better plugging capacity than the conventional gel systems under reservoir conditions. Hence, this gel system may be suitable in the water shutoff treatments required for enhanced oil recovery from oilfields.
基金Supported by the National ]qatural Science Foundation of China （20906064）, the National Basic Research Program of China （2009CB623407）, the Program for Changjiang Scholars and Innovative Research Team in University （IRTl163）, and the Foundation for the Author of National Excellent Doctoral Dissertation of China （201163）.
文摘Halloysite nanotube-composited thermo-responsive hydrogel system has been successfully developed for controlled drug release by copolymerization of N-isopropylacrylamide （NIPAM） with silane-modified halloysite nanotubes （HNT） through thermally initiated free-radical polymerization. With methylene blue as a model drug, thermo-responsive drug release results demonstrate that the drug release from the nanotubes in the composited hy-drogel can^be well controlled by manipulating the environmental temperature. When the hydrogel network is swol- len at temperature below the lower critical solution temperature （LCST）, drug releases steadily from lumens of the embedded nanotubes, whereas the drug release stops when hydrogel shrinks at temperature above the LCST. The release of model drug from the HNT-composited hydrogel matches well with its thermo-responsive volume phasetransition, and shows characteristics of well controlled release. The design strategy and release results of the pro- posed novel HNT-composited thermo-responsive hydrogel system provide valuable guidance for designing respon- s_i_ve nanocomposites for controlled-release of active agents.
基金the National Natural Science Founda-tion of China(82220108019 to P.Ji and 82201059 to Q.M.Zhai)the Chongqing Postdoctoral Science Special Foundation(2021XM1031 to Q.M.Zhai)+1 种基金China Postdoctoral Science Foundation(2022M720599 to Q.M.Zhai)Haobo medical technology(Shanghai)limited company helped us synthesize the PEGA materials.
文摘The impaired function of periodontal ligament stem cells(PDLSCs)impedes restoration of periodontal tissues.The cGAS-cGAMP-STING pathway is an innate immune pathway that sensing cytosolic double-stranded DNA(dsDNA),but its role in regulating the function of PDLSCs is still unclear.In this study,we found that mito-chondrial DNA(mtDNA)was released into the cytoplasm through the mitochondrial permeability transition pore(mPTP)in PDLSCs upon inflammation,which binds to cGAS and activated the STING pathway by promoting the production of cGAMP,and ultimately impaired the osteogenic differentiation of PDLSCs.Additionally,it is first found that inflammation can down-regulate the level of the ATP-binding cassette membrane subfamily member C1(ABCC1,a cGAMP exocellular transporter)and ectonucleotide pyrophosphatase/phosphodiesterase 1(ENPP1,a cGAMP hydrolase),which further aggravated the accumulation of intracellular cGAMP,leading to the persistent activation of the cGAS-STING pathway and thus the impaired the differentiation capacity of PDLSCs.Furthermore,we designed a hydrogel system loaded with a mPTP blocker,an ABCC1 agonist and ENPP1 to promote periodontal tissue regeneration by modulating the production,exocytosis,and clearance of cGAMP.In conclusion,our results highlight the profound effects,and specific mechanisms,of the cGAS-STING pathway on the function of stem cells and propose a new strategy to promote periodontal tissue restoration based on the reestablishment of cGAMP homeostasis.
基金supported by the Research Platform for biomedical and Health Technology, NUS (Suzhou) Research Institute (RP-BHT-Prof. LEE Chengkuo)RIE Advanced Manufacturing and Engineering (AME) Programmatic Grant (Grant A18A4b0055)+1 种基金RIE 2025-Industry Alignment Fund – Industry Collaboration Projects (IAF-ICP) (Grant I2301E0027)Reimagine Research Scheme projects, National University of Singapore, A-0009037-03-00 and A-0009454-01-00 and Reimagine Research Scheme projects, National University of Singapore, A-0004772-00-00 and A-0004772-01-00。
文摘Smart farming with outdoor monitoring systems is critical to address food shortages and sustainability challenges.These systems facilitate informed decisions that enhance efficiency in broader environmental management.Existing outdoor systems equipped with energy harvesters and self-powered sensors often struggle with fluctuating energy sources,low durability under harsh conditions,non-transparent or non-biocompatible materials,and complex structures.Herein,a multifunctional hydrogel is developed,which can fulfill all the above requirements and build selfsustainable outdoor monitoring systems solely by it.It can serve as a stable energy harvester that continuously generates direct current output with an average power density of 1.9 W m^(-3)for nearly 60 days of operation in normal environments(24℃,60%RH),with an energy density of around 1.36×10^(7)J m^(-3).It also shows good self-recoverability in severe environments(45℃,30%RH)in nearly 40 days of continuous operation.Moreover,this hydrogel enables noninvasive and self-powered monitoring of leaf relative water content,providing critical data on evaluating plant health,previously obtainable only through invasive or high-power consumption methods.Its potential extends to acting as other self-powered environmental sensors.This multifunctional hydrogel enables self-sustainable outdoor systems with scalable and low-cost production,paving the way for future agriculture.
基金the funding and generous support of the National Natural Science Foundation of China(52103263,52271249)the Key Project of International Science&Technology Cooperation of Shaanxi Province(2023-GHZD-09)+5 种基金the Key Project of Science Foundation of Education Department of Shaanxi Province(22JY011)the Key Project of Scientific Research and Development of Shaanxi Province(2023GXLH-070)the Qinchuangyuan"Scientist+Engineer"Team of Shaanxi Province(2023KXJ-069)the Key Research and Development Program of Shaanxi(2023-YBGY-488)the Sci-tech Innovation Team of Shaanxi Province(2024RS-CXTD-46)the Key Research and Development Program of Shaanxi Province(2020ZDLGY13-11).
文摘All-season thermal management with zero energy consumption and emissions is more crucial to global decarbonization over traditional energy-intensive cooling/heating systems.However,the static single thermal management for cooling or heating fails to self-regulate the temperature in dynamic seasonal temperature condition.Herein,inspired by the dual-temperature regulation function of the fur color changes on the backs and abdomens of penguins,a smart thermal management composite hydrogel(PNA@H-PM Gel)system was subtly created though an"on-demand"dual-layer structure design strategy.The PNA@H-PM Gel system features synchronous solar and thermal radiation modulation as well as tunable phase transition temperatures to meet the variable seasonal thermal requirements and energy-saving demands via self-adaptive radiative cooling and solar heating regulation.Furthermore,this system demonstrates superb modulations of both the solar reflectance(ΔR=0.74)and thermal emissivity(ΔE=0.52)in response to ambient temperature changes,highlighting efficient temperature regulation with average radiative cooling and solar heating effects of 9.6℃in summer and 6.1℃in winter,respectively.Moreover,compared to standard building baselines,the PNA@H-PM Gel presents a more substantial energy-saving cooling/heating potentials for energy-efficient buildings across various regions and climates.This novel solution,inspired by penguins in the real world,will offer a fresh approach for producing intelligent,energy-saving thermal management materials,and serve for temperature regulation under dynamic climate conditions and even throughout all seasons.
基金supported by the National Research Foundation of Korea(NRF)Grants funded by the Korea government(MSIT)(Nos.RS-2023-00213047 and RS-2024-00405818)。
文摘Artificial sensory systems,designed to emulate human senses like sight,touch,and hearing,have garnered significant attention for their potential to enhance human capabilities,improve human-machine interactions,and enable autonomous systems to better perceive their surroundings.Hydrogels,with their biocompatibility,flexibility,and water-rich polymer structure,are increasingly recognized as crucial materials in the development of these systems,especially in applications such as wearable sensors,artificial skin,and neural interfaces.This review explores various hydrogel fabrication techniques,including 3D bioprinting,electro spinning,and photopolymerization,which allow for the precise control of hydrogel properties like mechanical strength,flexibility,and conductivity.By tailoring these properties to mimic natural tissues,hydrogels offer transformative benefits in the creation of advanced,biocompatible,and durable sensory systems.We emphasize the importance of selecting appropriate fabrication methods to meet the specific functional requirements of artificial sensory applications,such as sensitivity to stimuli,durability,and ease of integration.This review further highlights the pivotal role of hydrogels in advancing future artificial sensory technologies and their broad potential in fields ranging from robotics to biomedical devices.
基金funded by the National Natural Science Foundation of China(grant No.82260596)the Natural Science Foundation of Jiangxi Province(grant No.20242BAB25506)+4 种基金the Science and Technology Program of Jiangxi Provincial Health and Family Planning Commission(grant No.202410246)the China Postdoctoral Science Foundation(grant No.2023M741523)he Science and Technology Program of Jiangxi Provincial Administration of Traditional Chinese Medicine(grant No.2024A0028)the Outstanding Youth Fund Program of Jiangxi Province(grant No.20252BAC220050)the Nanchang University Internal Funding Program Fund(grant No.2023efyB05).
文摘Traditional cancer therapies have limitations like poor efficacy on advanced tumors,healthy tissue damage,side effects,and drug resistance,creating an urgent need for new strategies.Hydrogels have good biocompatibility and controlled release,while extracellular vesicles(EVs)enable targeting and bioactive transport.This review systematically summarizes hydrogels and EVs,focusing on the construction of hydrogel-EV delivery system,key influencing factors,drug delivery mechanisms,and tumor therapy apps,clarifying their synergies.The system overcomes single-carrier flaws,construction methods/key factors affect performance,preclinical studies have confirmed efficacy in multiple therapies,but large-scale production and in vivo stability challenges remain,yet it promises to overcome the limits of traditional therapy for precision oncology.
基金supported by the National Institutes of Health(K01 AR07787,R21 R077700,P30 AR069619,R01 AR056624,R01 HL163168)National Science Foundation(CMMI 1548571)+2 种基金Department of Veterans Affairs(CReATE Motion Center,I50 RX004845)in the United Statessupported by the Korea Health Technology R&D Project through the Korea Health Industry Develop-ment Institute(KHIDI)funded by the Ministry of Health and Welfare(HI19C1095)National R&D Program through the National Research Foundation of Korea(NRF)funded by Ministry of Science and ICT(RS-2024-00405574)in the Republic of Korea.
文摘Meniscus injuries present significant therapeutic challenges due to their limited self-healing capacity and the diverse biological and mechanical properties across the tissue.Conventional repair strategies do not replicate the complex zonal characteristics within the meniscus,resulting in suboptimal outcomes.In this study,we introduce an innovative fetal/adult and stiffness-tunable meniscus decellularized extracellular matrix(DEM)-based hydrogel system designed for precision repair of heterogeneous,zonal-dependent meniscus injuries.By syn-thesizing fetal and adult DEM hydrogels,we identified distinct cellular responses,including that hydrogels with adult meniscus-derived DEM promote more fibrochondrogenic phenotypes.The incorporation of methacrylated hyaluronic acid(MeHA)further refined the mechanical properties and injectability of the DEM-based hydrogels.The combination of fetal and adult DEM with MeHA allowed for precise tuning of stiffness,influencing cell differentiation and closely mimicking native tissue environments.In vivo tests confirmed the biocompatibility of hydrogels and their integration with native meniscus tissues.Furthermore,advanced 3D bioprinting techniques enabled the fabrication of hybrid hydrogels with biomaterial and mechanical gradients,effectively emulating the zonal properties of meniscus tissue and enhancing cell integration.This study represents a significant advance in meniscus tissue engineering,providing a promising platform for customized regenerative therapies across a range of heterogeneous fibrous connective tissues.
基金the Department of Science and Technology(DST),Govt.of India for providing funds under the FIST program and PURSE grant vide No.SR/PURSE/2020/31 to the department of Chemistry,University of Kashmir.
文摘This study presents a thorough investigation into the use of single and twin-tailed cationic and anionic surfactant-modified chitosan(SMCS)hydrogel beads as effective adsorbents for the elimination of hazardous polycyclic aromatic hydrocarbons(PAHs)from aqueous solutions.The Chitosan(CS)hydrogel beads were modified with single/twin-tailed anionic surfactants,sodium dodecyl sulfate(SDS)and sodium bis(2-ethylhexyl)sulfosuccinate(AOT),and cationic surfactants,dodecyltrimethylammonium bromide(DTAB)and didodecyldimethylammonium bromide(DDAB),to enhance their adsorption capacity of PAHs.The CS and SMCS beads were evaluated for their structural,mechanical,and adsorption properties using a range of techniques,including infrared spectroscopy(IR),energy-dispersive X-ray spectroscopy(EDX),rheometry,and field emission scanning electron microscopy(FESEM).Adsorption experiments of naphthalene(Nap),acenaphthene(Ace),and phenanthrene(Phe)on SMCS beads demonstrate that they have significantly higher adsorption capacities than CS beads,due to increase in hydrophobic interactions.Adsorption capacity followed the trend,Phen>Ace>Nap for all the beads revealing that twin-tailed SMCS bead possess much higher adsorption capacities(Qmax)compared to single-tailed SMCS beads.For twin tailed surfactants,the maximum adsorption capacities for Nap,Ace and Phe varied as CS-AOT(CS-DDAB):430.0(323.8)611.60(538.18)633.39(536.99)mg/g respectively,outperforming other reported hydrogel beads.The study highlights the simplicity,eco-friendliness,and enhanced performance of surfactant modification for developing high-efficiency adsorbents,paving the way for cost-effective solutions in water re-mediation.
基金financially supported by the Science and Technology Innovation Program of Hunan Province(2024RC3003)the Central South University Innovation-Driven Research Programme(2023CXQD012)the Initiative for Sustainable Energy for its financial support。
文摘By combining the merits of radiative cooling(RC)and evaporation cooling(EC),radiative coupled evaporative cooling(REC)has attracted considerable attention for sub-ambient cooling purposes.However,for outdoor devices,the interior heating power would increase the working temperature and fire risk,which would suppress their above-ambient heat dissipation capabilities and passive water cycle properties.In this work,we introduced a REC design based on an all-in-one photonic hydrogel for above-ambient heat dissipation and flame retardancy.Unlike conventional design RC film for heat dissipation with limited cooling power and fire risk,REC hydrogel can greatly improve the heat dissipation performance in the daytime with a high workload,indicating a 12.0℃lower temperature than the RC film under the same conditions in the outdoor experiment.In the nighttime with a low workload,RC-assisted adsorption can improve atmospheric water harvesting to ensure EC in the daytime.In addition,our REC hydrogel significantly enhanced flame retardancy by absorbing heat without a corresponding temperature rise,thus mitigating fire risks.Thus,our design shows a promising solution for the thermal management of outdoor devices,delivering outstanding performance in both heat dissipation and flame retardancy.
基金supported by National Natural Science Foundation of China(22209036,U23A20119)Hebei Provincial Natural Science Foundation,Excellent Youth Project(E2023202069)+1 种基金National Key R&D Program of China(2024YFF0506000,2024YFB4609100)Fundamental Research Foundation from Hebei University of Technology(424132016,282021485).
文摘Solar-driven interfacial desalination(SID)offers a sustainable route for freshwater production,yet its long-term performance is compromised by salt crystallization and microbial fouling under complex marine conditions.Zwitterionic polymers offer promising nonfouling capabilities,but current zwitterionic hydrogel-based solar evaporators(HSEs)suffer from inadequate hydration and salt vulnerability.Inspired by the natural marine environmental adaptive characteristics of saltwater fish,we report a superhydrated zwitterionic poly(trimethylamine N-oxide,PTMAO)/polyacrylamide(PAAm)/polypyrrole(PPy)hydrogel(PTAP)with dedicated water channels for efficient,durable,and nonfouling SID.The directly linked N⁺and O⁻groups in PTMAO establish a robust hydration shell that facilitates rapid water transport while resisting salt and microbial adhesion.Integrated PAAm and PPy networks enhance mechanical strength and photothermal conversion.PTAP achieves a high evaporation rate of 2.35 kg m^(−2)h^(−1)under 1 kW m^(–2)in 10 wt%NaCl solution,maintaining stable operation over 100 h without salt accumulation.Furthermore,PTAP effectively resists various foulants including proteins,bacterial,and algal adhesion.Molecular dynamics simulations reveal that the exceptional hydration capacity supports its nonfouling properties.This work advances the development of nonfouling HSEs for sustainable solar desalination in real-world marine environments.
基金the financial support from the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB0470303)the National Key Research and Development Program of China (2022YFB4600101)+5 种基金the National Natural Science Foundation of China (52175201)the Research Program of Science and Technology Department of Gansu Province (24JRRA059, 24JRRA044, and 24YFFA014)the Science Fund of Shandong Laboratory of Advanced Materials and Green Manufacturing at Yantai (AMGM2024F12)the Major Program (ZYFZFX-2) of the Lanzhou Institute of Chemical Physics, CASthe Special Research Assistant Project of the Chinese Academy of Sciencesthe Oasis Scholar of Shihezi University
文摘Octopuses,due to their flexible arms,marvelous adaptability,and powerful suckers,are able to effortlessly grasp and disengage various objects in the marine surrounding without causing devastation.However,manipulating delicate objects such as soft and fragile foods underwater require gentle contact and stable adhesion,which poses a serious challenge to now available soft grippers.Inspired by the sucker infundibulum structure and flexible tentacles of octopus,herein we developed a hydraulically actuated hydrogel soft gripper with adaptive maneuverability by coupling multiple hydrogen bond-mediated supramolecular hydrogels and vat polymerization three-dimensional printing,in which hydrogel bionic sucker is composed of a tunable curvature membrane,a negative pressure cavity,and a pneumatic chamber.The design of the sucker structure with the alterable curvature membrane is conducive to realize the reliable and gentle switchable adhesion of the hydrogel soft gripper.As a proof-of-concept,the adaptive hydrogel soft gripper is capable of implement diversified underwater tasks,including gingerly grasping fragile foods like egg yolks and tofu,as well as underwater robots and vehicles that station-keeping and crawling based on switchable adhesion.This study therefore provides a transformative strategy for the design of novel soft grippers that will render promising utilities for underwater exploration soft robotics.
基金supported by the Shenzhen Hong Kong Joint Funding Project,No.SGDX20230116093645007(to LY)the Shenzhen Science and Technology Innovation Committee International Cooperation Project,No.GJHZ20200731095608025(to LY)+7 种基金Shenzhen Development and Reform Commission’s Intelligent Diagnosis,Treatment and Prevention of Adolescent Spinal Health Public Service Platform,No.S2002Q84500835(to LY)Shenzhen Medical Research Fund,No.B2303005(to LY)Team-based Medical Science Research Program,No.2024YZZ02(to LY)Zhejiang Provincial Natural Science Foundation of China,No.LWQ20H170001(to RL)Basic Research Project of Shenzhen Science and Technology from Shenzhen Science and Technology Innovation Commission,No.JCYJ20210324103010029(to BY)Shenzhen Second People’s Hospital Clinical Research Fund of Guangdong Province High-level Hospital Construction Project,Nos.2023yjlcyj029(to BY),2023yjlcyj021(to LL)Guangdong Basic and Applied Basic Research Foundation,No.2022A1515110679(to LL)China Postdoctoral Science Foundation,No.2022M722203(to GL).
文摘Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge.Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological properties and tissue specificity,highlighting its potential as a biomedical material for the repair of severe peripheral nerve injury;however,its role in modulating neuroinflammation post-peripheral nerve injury remains unknown.Here,we aimed to characterize the anti-inflammatory properties of porcine decellularized nerve matrix hydrogel and their underlying molecular mechanisms.Using peripheral nerve injury model rats treated with porcine decellularized nerve matrix hydrogel,we evaluated structural and functional recovery,macrophage phenotype alteration,specific cytokine expression,and changes in related signaling molecules in vivo.Similar parameters were evaluated in vitro using monocyte/macrophage cell lines stimulated with lipopolysaccharide and cultured on porcine decellularized nerve matrix hydrogel-coated plates in complete medium.These comprehensive analyses revealed that porcine decellularized nerve matrix hydrogel attenuated the activation of excessive inflammation at the early stage of peripheral nerve injury and increased the proportion of the M2 subtype in monocytes/macrophages.Additionally,porcine decellularized nerve matrix hydrogel negatively regulated the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB axis both in vivo and in vitro.Our findings suggest that the efficacious anti-inflammatory properties of porcine decellularized nerve matrix hydrogel induce M2 macrophage polarization via suppression of the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB pathway,providing new insights into the therapeutic mechanism of porcine decellularized nerve matrix hydrogel in peripheral nerve injury.
基金supported by European Union Funding Programme,PNRR,No. 760058(to DMH)the UEFISCDI Project,No. PN-III-P4-IDPCE-2020-059(to APW)
文摘The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly formed neurons to the damaged area.However,only a small percentage of these neurons survive,and many do not reach the damaged area,possibly because the corpus callosum impedes the migration of subventricular zone-derived stem cells into the lesioned cortex.A second major obstacle to stem cell therapy is the strong inflammatory reaction induced by cerebral ischemia,whereby the associated phagocytic activity of brain macrophages removes both therapeutic cells and/or cell-based drug carriers.To address these issues,neurogenesis was electrically stimulated in the subventricular zone,followed by isolation of proliferating cells,including newly formed neurons,which were subsequently mixed with a nutritional hydrogel.This mixture was then transferred to the stroke cavity of day 14 post-stroke mice.We found that the performance of the treated animals improved in behavioral tests,including novel object,open field,hole board,grooming,and“time-to-feel”adhesive tape tests.Furthermore,immunostaining revealed that the stem cell marker nestin,the neuroepithelial marker Mash1,and the immature neuronal marker doublecortin-positive cells survived in the transplanted area for 2 weeks,possibly due to reduced phagocytic activity and supportive angiogenesis.These results clearly indicate that the transplantation of committed subventricular zone stem cells combined with a protective nutritional gel directly into the infarct cavity after the peak of stroke-induced neuroinflammation represents a feasible approach to improve neurorestoration after cerebral ischemia.
基金This research is financially supported by Korea Institute of Science and Technology(2E31121)Korea National Research Foundation(2018M3A9H1024872).
文摘Treatment of osteoarthritis(OA)by administration of corticosteroids is a commonly used method in clinics using anti-inflammatory medicine.Oral administration or intra-articular injection of corticosteroids can reduce the pain and progress of cartilage degeneration,but they are usually insufficient to show local and long-term anti-inflammatory effects because of their fast clearance in the body.In this study,we suggest an injectable anti-OA drug depot system for sustained drug release that provides long-term effective therapeutic advantages.Amphiphilic poly(organophosphazene),which has temperature-dependent nanoparticle forming and sol-gel transition behaviors when dissolved in aqueous solution,was synthesized for triamcinolone acetonide(TCA)delivery.Because hydrophobic parts of the polymer can interact with hydrophobic parts of the TCA,the TCA was encapsulated into the self-assembled polymeric nanoparticles.The TCA-encapsulated polymeric nanoparticles(TePNs)were well dispersed in an aqueous solution below room temperature so that they can be easily injected as a sol state into an intra-articular region.However,the TePNs solution transforms immediately to a viscose 3D hydrogel like a synovial fluid in the intra-articular region via the conducted body temperature.An in vitro TCA release study showed sustained TCA release for six weeks.One-time injection of the TePN hydrogel system in an early stage of OA-induced rat model showed a great inhibition effect against further OA progression.The OA-induced knees completely recovered as a healthy cartilage without any abnormal symptoms.
基金Areas of Excellence Scheme(AoE/M402/20)Theme-based Research Scheme(T13-402/17 N)under the Research Grant Council of Hong Kong,Mainland-Hong Kong Joint Funding Scheme(MHP/030/20)+4 种基金National Natural Science Foundation of China(81802152 and 32171332)Natural Science Foundation of Guangdong Province(2019A1515012224)Research Grants Council Collaborative Research Fund(C4026-17WF)General Research Fund(14121918 and 14173917)Innovation and Technology Commission Funding(Ref No.ITS/208/18FX).
文摘Mandibular defect becomes a prevalent maxillofacial disease resulting in mandibular dysfunctions and huge psychological burdens to the patients.Considering the routine presence of oral contaminations and aesthetic restoration of facial structures,the current clinical treatments are however limited,incapable to reconstruct the structural integrity and regeneration,spurring the need for cost-effective mandibular tissue engineering.Hydrogel systems possess great merit for mandibular reconstruction with precise involvement of cells and bioactive factors.In this review,current clinical treatments and distinct mode(s)of mandible formation and pathological resorption are summarized,followed by a review of hydrogel-related mandibular tissue engineering,and an update on the advanced fabrication of hydrogels with improved mechanical property,antibacterial ability,injectable form,and 3D bioprinted hydrogel constructs.The exploration of advanced hydrogel systems will lay down a solid foundation for a bright future with more biocompatible,effective,and personalized treatment in mandibular reconstruction.
基金This work was financially supported by the National Natural Science Foundation of China(31930067,31525009,31800797 and 31771096)the National Key Research and Development Program of China(2017YFC1103502)+1 种基金China Postdoctoral Science Foundation(2018M631094 and 2019M653410)1⋅3⋅5project for disciplines of excellence,West China Hospital,Sichuan University(ZYGD18002).
文摘Although neoantigen-based cancer vaccines show great potential in cancer immunotherapy due to their ability to induce effective and long-lasting anti-tumor immunity,their development is hindered by the limitations of neoantigens identification,low immunogenicity,and weak immune response.Cyclophosphamide(CTX)not only directly kills tumors but also causes immunogenic cell death,providing a promising source of antigens for cancer vaccines.Herein,a combined immunotherapy strategy based on temperature-sensitive PLEL hydrogel is designed.First,CTX-loaded hydrogel is injected intratumorally into CT26 bearing mice to prime anti-tumor immunity,and then 3 days later,PLEL hydrogels loaded with CpG and tumor lysates are subcutaneously injected into both groins to further promote anti-tumor immune responses.The results confirm that this combined strategy reduces the toxicity of CTX,and produces the cytotoxic T lymphocyte response to effectively inhibit tumor growth,prolong survival,and significantly improve the tumor cure rate.Moreover,a long-lasting immune memory response is observed in the mice.About 90%of the cured mice survive for at least 60 days after being re-inoculated with tumors,and the distant tumor growth is also well inhibited.Hence,this PLEL-based combination therapy may provide a promising reference for the clinical promotion of chemotherapy combined with cancer vaccines.
