Background:Hydrastis canadensis mother tincture(HC-MT)is a preparation derived from the roots of the Hydrastis canadensis plant,commonly known as goldenseal.Despite its widespread use in homeopathic clinics for cancer...Background:Hydrastis canadensis mother tincture(HC-MT)is a preparation derived from the roots of the Hydrastis canadensis plant,commonly known as goldenseal.Despite its widespread use in homeopathic clinics for cancer treatment,there has been a lack of molecular studies assessing its efficacy against cancer.The aim of this study is to investigate the apoptotic and anti-metastatic properties of HC-MT in human lung adenocarcinoma cells(A549).Methods:MTT and LDH assays were conducted to assess the cytotoxic potential of HC-MT on tumor cells.Various staining techniques were employed to investigate the apoptotic potential,which was then confirmed through DNA fragmentation assay and western blotting.Results:The results showed that the HC-MT exhibited anti-proliferative effects with an IC50 concentration of 13.16μL/mL with apoptosis as the predominant mode of cell death.Treatment with HC-MT at IC50 concentration resulted in cell cycle arrest at the G1 phase.Additionally,HC-MT exhibited anti-migratory properties.Conclusion:In summary,HC-MT demonstrated significant anti-cancer effects in lung cancer cells,inducing apoptosis primarily and arresting cell cycle progression at G1 phase mediated by ROS.It also exhibited anti-metastatic potential,with its active compound berberine showing interaction with key proteins involved in cancer progression.These findings suggest a promising therapeutic potential of HC-MT against lung cancer.展开更多
文摘Background:Hydrastis canadensis mother tincture(HC-MT)is a preparation derived from the roots of the Hydrastis canadensis plant,commonly known as goldenseal.Despite its widespread use in homeopathic clinics for cancer treatment,there has been a lack of molecular studies assessing its efficacy against cancer.The aim of this study is to investigate the apoptotic and anti-metastatic properties of HC-MT in human lung adenocarcinoma cells(A549).Methods:MTT and LDH assays were conducted to assess the cytotoxic potential of HC-MT on tumor cells.Various staining techniques were employed to investigate the apoptotic potential,which was then confirmed through DNA fragmentation assay and western blotting.Results:The results showed that the HC-MT exhibited anti-proliferative effects with an IC50 concentration of 13.16μL/mL with apoptosis as the predominant mode of cell death.Treatment with HC-MT at IC50 concentration resulted in cell cycle arrest at the G1 phase.Additionally,HC-MT exhibited anti-migratory properties.Conclusion:In summary,HC-MT demonstrated significant anti-cancer effects in lung cancer cells,inducing apoptosis primarily and arresting cell cycle progression at G1 phase mediated by ROS.It also exhibited anti-metastatic potential,with its active compound berberine showing interaction with key proteins involved in cancer progression.These findings suggest a promising therapeutic potential of HC-MT against lung cancer.
