[Objective] The research aimed to study the toxicity effect of microsystin on Hydra（aquatic animal model）.[Method] Hydra magnipapillata was as the experimental material.Under the different concentrations of MC-LR so...[Objective] The research aimed to study the toxicity effect of microsystin on Hydra（aquatic animal model）.[Method] Hydra magnipapillata was as the experimental material.Under the different concentrations of MC-LR solution,its acute toxicity effect on Hydra magnipapillata was observed.[Result] The half lethal concentration（LC50） of MC-LR on Hydra magnipapillata at 24 h was 12.158,4.029 mg/L at 48 h and 1.799 mg/L at 72 h.[Conclusion] Hydra magnipapillata would die when treated by the low concentration of MC-LR solution.It illustrated that MC-LR produced by algal blooms caused the damage on some aquatic animals,and even some low-level aquatic animals died,such as Hydra magnipapillata and so on.The research provided some reference basis for the detection and assessment of water body pollution.展开更多
Practicability of method for the Hydra regeneration assay on the prescreening teratogenic potential of chlorinated drinking water disinfection by products was studied through both the assays of toxicity of adult ...Practicability of method for the Hydra regeneration assay on the prescreening teratogenic potential of chlorinated drinking water disinfection by products was studied through both the assays of toxicity of adult Hydra (T) and inhibition of the growth of regeneration Hydra (I) by using chloroform, dichloromethane and chloroacetic acid. The results showed that T 50 / I 50 ratios of chloroform and chloroacetic acid were 2 77 and 6 16 respectively, with teratogenic potential. T 50 / I 50 ratio of dichloromethane was 1.69, with weaker teratogenic potential. These experimental results indicated preliminarily that the Hydra regeneration assay has certainly applied value as a prescreening assay for developmental toxicity.展开更多
Metalloproteinases have a critical role in a broad spectrum of cellular processes ranging from the break-down of extracellulax matrix to the processing of signal transduction-related proteins. These hydrolyticfunction...Metalloproteinases have a critical role in a broad spectrum of cellular processes ranging from the break-down of extracellulax matrix to the processing of signal transduction-related proteins. These hydrolyticfunctions underlie a variety of mechanisms related to developmental processes as well as disease states.Structural analysis of metalloproteinases from both invertebrate and vertebrate species indicates that theseenzymes are highly conserved and arose early during metazoan evolution. In this regard, studies from vari-ous laboratories have reported that a number of classes of metalloproteinases are found in hydra, a memberof Cnidaria, the second oldest of existing animal phyla. These studies demonstrate that the hydra genomecontains at least three classes of metalloproteinases to include members of the 1) astacin class, 2) matrix met-alloproteinase class, and 3) neprilysin class. Functional studies indicate that these metalloproteinases playdiverse and important roles in hydra morphogenesis and cell differentiation as well as specialized functionsin adult polyps. This article will review the structure, expression, and function of these metalloproteinasesin hydra.展开更多
The fresh water polyp Hydra belongs to the phylum Cnidaria, which diverged from the metazoan lineage before the appearance of bilaterians. In order to understand the evolution of apoptosis in metazoans, we have begun ...The fresh water polyp Hydra belongs to the phylum Cnidaria, which diverged from the metazoan lineage before the appearance of bilaterians. In order to understand the evolution of apoptosis in metazoans, we have begun to elucidate the molecular cell death machinery in this model organism. Based on ESTs and the whole Hydra genome assembly, we have identified 15 caspases. We show that one is activated during apoptosis, four have characteristics of initiator caspases with N-terminal DED, CARD or DD domain and two undergo autoprocessing in vitro. In addition, we describe seven Bcl-2-1ike and two Bak-like proteins. For most of the Bcl-2 family proteins, we have observed mitochondrial localization. When expressed in mammalian cells, HyBak-like 1 and 2 strongly induced apoptosis. Six of the Bcl-2 family members inhibited apoptosis induced by camptothecin in mammalian ceils with HyBcl-2-1ike 4 showing an especially strong protective effect. This protein also interacted with HyBak-like 1 in a yeast two-hybrid assay. Mutation of the conserved leucine in its BH3 domain abolished both the interaction with HyBak-like 1 and the anti-apoptotic effect. Moreover, we describe novel Hydra BH-3-only proteins. One of these interacted with Bcl-2-1ike 4 and induced apoptosis in mammalian cells. Our data indicate that the evolution of a complex network for cell death regulation arose at the earliest and simplest level of multicellular organization, where it exhibited a substantially higher level of complexity than in the protostome model organisms Caenorhabditis and Drosophila.展开更多
The Hydrangea genus belongs to the Hydrangeaceae family,in the Cornales order of flowering plants,which early diverged among the Asterids,and includes several species that are commonly used ornamental plants.Of them,H...The Hydrangea genus belongs to the Hydrangeaceae family,in the Cornales order of flowering plants,which early diverged among the Asterids,and includes several species that are commonly used ornamental plants.Of them,Hydrangea macrophylla is one of the most valuable species in the nursery trade,yet few genomic resources are available for this crop or closely related Asterid species.Two high-quality haplotype-resolved reference genomes of hydrangea cultivars‘Veitchii’and‘Endless Summer’[highest quality at 2.22 gigabase pairs(Gb),396 contigs,N5022.8 megabase pairs(Mb)]were assembled and scaffolded into the expected 18 pseudochromosomes.Utilizing the newly developed high-quality reference genomes along with high-quality genomes of other related flowering plants,nuclear data were found to support a single divergence point in the Asterids clade where both the Cornales and Ericales diverged from the euasterids.Genetic mapping with an F1 hybrid population demonstrated the power of linkage mapping combined with the new genomic resources to identify the gene for inflorescence shape,CYP78A5 located on chromosome 4,and a novel gene,BAM3 located on chromosome 17,for causing double flower.Resources developed in this study will not only help to accelerate hydrangea genetic improvement but also contribute to understanding the largest group of flowering plants,the Asterids.展开更多
Metalloproteinases have a critical role in a broad spectrum of cellular processesranging from the break-down of extracellular matrix to the processing of signaltransduction-related proteins.These hydrolytic functions ...Metalloproteinases have a critical role in a broad spectrum of cellular processesranging from the break-down of extracellular matrix to the processing of signaltransduction-related proteins.These hydrolytic functions underlie a variety of mechanisms related to developmental processes as well as disease states.Structural analysis of metalloproteinases from both invertebrate and vertebrate species indicates that these enzymes are highly conserved and arose early during metazoan evolution.In this regard,studies from vari-ous laboratories have reported that a number of classes of metalloproteinases are found in hydra,a member of Cnidaria,the second oldest of existing animal phyla.These studies demonstrate that the hydra genome contains at least three classes of metalloproteinases to include members of the 1)astacin class,2)matrix met-alloproteinase class,and 3)neprilysin class.Functional studies indicate that these metalloproteinases play diverse and important roles in hydra morphogenesis and cell differentiation as well as specialized functions in adult polyps.This article will review the structure,expression,and function of these metalloproteinases in hydra.展开更多
The synaptonemal complex （SC） is an evolutionarily well-conserved structure that mediates chromosome synapsis during prophase of the first meiotic division. Although its structure is conserved, the characterized pro...The synaptonemal complex （SC） is an evolutionarily well-conserved structure that mediates chromosome synapsis during prophase of the first meiotic division. Although its structure is conserved, the characterized protein components in the current metazoan meiosis model systems （Drosophila melanogaster, Caenorhabditis elegans, and Mus musculus） show no sequence homology, challenging the question of a single evolutionary origin of the SC. However, our recent studies revealed the monophyletic origin of the mammalian SC protein components. Many of them being ancient in Metazoa and already present in the cnidarian Hydra. Remarkably, a comparison between different model systems disclosed a great similarity between the SC components of Hydra and mammals while the proteins of the ecdysozoan systems （D. rnelanogaster and C. elegans） differ significantly. In this review, we introduce the basal-branching metazoan species Hydra as a potential novel invertebrate model system for meiosis research and particularly for the investigation of SC evolution, function and assembly. Also, available methods for SC research in Hydra are summarized.展开更多
Due to high construction material cost in Ethiopia,it is difficult to afford a shelter for most people.The hydra form block(HFB)has been identified as low-cost building material with its potential and possibility to r...Due to high construction material cost in Ethiopia,it is difficult to afford a shelter for most people.The hydra form block(HFB)has been identified as low-cost building material with its potential and possibility to reverse the housing problem.Laboratory tests were conducted on Mettu,Nopa,Gore,and Hurumu areas soil.Using hydra form machine with average mold size of 29*14*10 cm,hydra form blocks were cast with the three percentages increment of cement.Compressive strength and water absorption tests were conducted at 28 day.The investigation has revealed that all the soil sample except Gore soil have significant characteristics that make it suitable for stabilization with recommended soil properties.From the experimental study,all the blocks except blocks produced with Gore soil have 28th day compressive strength values well above most of the recommended minimum values.Water absorption was less than the maximum limit of 15%.But,for control block and for stabilized with 3%cement,water absorption result is out of the recommended values(0-15)%.The cost comparison of hydra form blocks with hollow concrete block and fired clay brick shows that the hydra form block is cheapest walling material in terms of production cost and a typical hydra form block production center can create jobs for more than 50 people.展开更多
基金Supported by National Natural Science Foundation of China(40972092)~~
文摘[Objective] The research aimed to study the toxicity effect of microsystin on Hydra（aquatic animal model）.[Method] Hydra magnipapillata was as the experimental material.Under the different concentrations of MC-LR solution,its acute toxicity effect on Hydra magnipapillata was observed.[Result] The half lethal concentration（LC50） of MC-LR on Hydra magnipapillata at 24 h was 12.158,4.029 mg/L at 48 h and 1.799 mg/L at 72 h.[Conclusion] Hydra magnipapillata would die when treated by the low concentration of MC-LR solution.It illustrated that MC-LR produced by algal blooms caused the damage on some aquatic animals,and even some low-level aquatic animals died,such as Hydra magnipapillata and so on.The research provided some reference basis for the detection and assessment of water body pollution.
文摘Practicability of method for the Hydra regeneration assay on the prescreening teratogenic potential of chlorinated drinking water disinfection by products was studied through both the assays of toxicity of adult Hydra (T) and inhibition of the growth of regeneration Hydra (I) by using chloroform, dichloromethane and chloroacetic acid. The results showed that T 50 / I 50 ratios of chloroform and chloroacetic acid were 2 77 and 6 16 respectively, with teratogenic potential. T 50 / I 50 ratio of dichloromethane was 1.69, with weaker teratogenic potential. These experimental results indicated preliminarily that the Hydra regeneration assay has certainly applied value as a prescreening assay for developmental toxicity.
基金The authors wish to thank Eileen Roach for as-sistance with the preparation of the figures used inthis article. This work was supported by the fOllow-ing grants: AR39189 (HN) DK61373 (MPS) Amer-ican Heart Associate grant 0051436Z (MPS).
文摘Metalloproteinases have a critical role in a broad spectrum of cellular processes ranging from the break-down of extracellulax matrix to the processing of signal transduction-related proteins. These hydrolyticfunctions underlie a variety of mechanisms related to developmental processes as well as disease states.Structural analysis of metalloproteinases from both invertebrate and vertebrate species indicates that theseenzymes are highly conserved and arose early during metazoan evolution. In this regard, studies from vari-ous laboratories have reported that a number of classes of metalloproteinases are found in hydra, a memberof Cnidaria, the second oldest of existing animal phyla. These studies demonstrate that the hydra genomecontains at least three classes of metalloproteinases to include members of the 1) astacin class, 2) matrix met-alloproteinase class, and 3) neprilysin class. Functional studies indicate that these metalloproteinases playdiverse and important roles in hydra morphogenesis and cell differentiation as well as specialized functionsin adult polyps. This article will review the structure, expression, and function of these metalloproteinasesin hydra.
文摘The fresh water polyp Hydra belongs to the phylum Cnidaria, which diverged from the metazoan lineage before the appearance of bilaterians. In order to understand the evolution of apoptosis in metazoans, we have begun to elucidate the molecular cell death machinery in this model organism. Based on ESTs and the whole Hydra genome assembly, we have identified 15 caspases. We show that one is activated during apoptosis, four have characteristics of initiator caspases with N-terminal DED, CARD or DD domain and two undergo autoprocessing in vitro. In addition, we describe seven Bcl-2-1ike and two Bak-like proteins. For most of the Bcl-2 family proteins, we have observed mitochondrial localization. When expressed in mammalian cells, HyBak-like 1 and 2 strongly induced apoptosis. Six of the Bcl-2 family members inhibited apoptosis induced by camptothecin in mammalian ceils with HyBcl-2-1ike 4 showing an especially strong protective effect. This protein also interacted with HyBak-like 1 in a yeast two-hybrid assay. Mutation of the conserved leucine in its BH3 domain abolished both the interaction with HyBak-like 1 and the anti-apoptotic effect. Moreover, we describe novel Hydra BH-3-only proteins. One of these interacted with Bcl-2-1ike 4 and induced apoptosis in mammalian cells. Our data indicate that the evolution of a complex network for cell death regulation arose at the earliest and simplest level of multicellular organization, where it exhibited a substantially higher level of complexity than in the protostome model organisms Caenorhabditis and Drosophila.
基金The research presented in this article was supported in part by funds from the Floral and Nursery Research Initiative administered through the United States Department of Agriculture,Agricultural Research Service(USDA-ARS).Theworkwas supported by USDA-ARS CRIS project numbers 6066-21310-005-00D and 8020-21000-072-000-DThe authors would like to thank Carrie Witcher for assistance with pollen collection and microscopy and Dr Jerry Jenkins from the HudsonAlpha Institute for Biotechnology for his technical advice on scaffolding.BenjaminMoore and Joseph Davis maintained the plants used in this experiment.This research used resources provided by the SCINet project of the USDA Agricultural Research Service,ARS project numbers 0500-00093-001-00-D and 5030-21000-069-00D.The mention of trade names of commercial products in the publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.
文摘The Hydrangea genus belongs to the Hydrangeaceae family,in the Cornales order of flowering plants,which early diverged among the Asterids,and includes several species that are commonly used ornamental plants.Of them,Hydrangea macrophylla is one of the most valuable species in the nursery trade,yet few genomic resources are available for this crop or closely related Asterid species.Two high-quality haplotype-resolved reference genomes of hydrangea cultivars‘Veitchii’and‘Endless Summer’[highest quality at 2.22 gigabase pairs(Gb),396 contigs,N5022.8 megabase pairs(Mb)]were assembled and scaffolded into the expected 18 pseudochromosomes.Utilizing the newly developed high-quality reference genomes along with high-quality genomes of other related flowering plants,nuclear data were found to support a single divergence point in the Asterids clade where both the Cornales and Ericales diverged from the euasterids.Genetic mapping with an F1 hybrid population demonstrated the power of linkage mapping combined with the new genomic resources to identify the gene for inflorescence shape,CYP78A5 located on chromosome 4,and a novel gene,BAM3 located on chromosome 17,for causing double flower.Resources developed in this study will not only help to accelerate hydrangea genetic improvement but also contribute to understanding the largest group of flowering plants,the Asterids.
文摘Metalloproteinases have a critical role in a broad spectrum of cellular processesranging from the break-down of extracellular matrix to the processing of signaltransduction-related proteins.These hydrolytic functions underlie a variety of mechanisms related to developmental processes as well as disease states.Structural analysis of metalloproteinases from both invertebrate and vertebrate species indicates that these enzymes are highly conserved and arose early during metazoan evolution.In this regard,studies from vari-ous laboratories have reported that a number of classes of metalloproteinases are found in hydra,a member of Cnidaria,the second oldest of existing animal phyla.These studies demonstrate that the hydra genome contains at least three classes of metalloproteinases to include members of the 1)astacin class,2)matrix met-alloproteinase class,and 3)neprilysin class.Functional studies indicate that these metalloproteinases play diverse and important roles in hydra morphogenesis and cell differentiation as well as specialized functions in adult polyps.This article will review the structure,expression,and function of these metalloproteinases in hydra.
基金supported by a grant from the Priority Program of the German Science Foundation (SPP 1384,Mechanisms of Genome Haploidization)
文摘The synaptonemal complex （SC） is an evolutionarily well-conserved structure that mediates chromosome synapsis during prophase of the first meiotic division. Although its structure is conserved, the characterized protein components in the current metazoan meiosis model systems （Drosophila melanogaster, Caenorhabditis elegans, and Mus musculus） show no sequence homology, challenging the question of a single evolutionary origin of the SC. However, our recent studies revealed the monophyletic origin of the mammalian SC protein components. Many of them being ancient in Metazoa and already present in the cnidarian Hydra. Remarkably, a comparison between different model systems disclosed a great similarity between the SC components of Hydra and mammals while the proteins of the ecdysozoan systems （D. rnelanogaster and C. elegans） differ significantly. In this review, we introduce the basal-branching metazoan species Hydra as a potential novel invertebrate model system for meiosis research and particularly for the investigation of SC evolution, function and assembly. Also, available methods for SC research in Hydra are summarized.
文摘Due to high construction material cost in Ethiopia,it is difficult to afford a shelter for most people.The hydra form block(HFB)has been identified as low-cost building material with its potential and possibility to reverse the housing problem.Laboratory tests were conducted on Mettu,Nopa,Gore,and Hurumu areas soil.Using hydra form machine with average mold size of 29*14*10 cm,hydra form blocks were cast with the three percentages increment of cement.Compressive strength and water absorption tests were conducted at 28 day.The investigation has revealed that all the soil sample except Gore soil have significant characteristics that make it suitable for stabilization with recommended soil properties.From the experimental study,all the blocks except blocks produced with Gore soil have 28th day compressive strength values well above most of the recommended minimum values.Water absorption was less than the maximum limit of 15%.But,for control block and for stabilized with 3%cement,water absorption result is out of the recommended values(0-15)%.The cost comparison of hydra form blocks with hollow concrete block and fired clay brick shows that the hydra form block is cheapest walling material in terms of production cost and a typical hydra form block production center can create jobs for more than 50 people.
