Dear Editor,This letter studies finite-time stability (FTS) of impulsive and switched hybrid systems with delay-dependent impulses. Some conditions, based on Lyapunov method, are proposed for ensuring FTS and estimati...Dear Editor,This letter studies finite-time stability (FTS) of impulsive and switched hybrid systems with delay-dependent impulses. Some conditions, based on Lyapunov method, are proposed for ensuring FTS and estimating settling-time function (STF) of the hybrid systems.When switching dynamics are FTS and impulsive dynamics involve destabilizing delay-dependent impulses, the FTS is retained if the impulses occur infrequently.展开更多
This study integrates the individual photovoltaic(PV)and thermoelectric generator(TEG)systems into a PV-TEG hybrid system to improve its overall power output by reutilizing the waste heat generated during PV power pro...This study integrates the individual photovoltaic(PV)and thermoelectric generator(TEG)systems into a PV-TEG hybrid system to improve its overall power output by reutilizing the waste heat generated during PV power production to enhance its operational relia-bility.However,stochastic environmental conditions often result in partial shading conditions and nonuniform thermal distribution across the PV-TEG modules,which negatively affect the output characteristics of the system,thus presenting a significant challenge to maintaining their optimal performance.To address these challenges,a novel fitness-distance-balance-based beluga whale optimization(FDBBWO)strategy has been devised for maximizing the power output of the PV-TEG hybrid system under dynamic operation scenar-ios.A broader spectrum of complex and authentic operational contexts has been considered in case studies to examine the effectiveness and feasibility of FDBBWO.For this,real-world datasets collected from different seasons in Hong Kong have been used to validate the practical viability of the proposed strategy.Simulation results reveal that the FDBBWO based maximum power point tracking technique outperforms its competing methods by achieving the highest energy output,with a remarkable increase of up to 134.25%with minimal power fluctuations.For instance,the energy obtained by FDBBWO is 47.45%and 58.34%higher than BWO and perturb and observe methods,respectively,in the winter season.展开更多
This research pioneers the integration of geographic information systems(GIS)and 3D modeling within a virtual reality(VR)framework to assess the viability and planning of a 20 MW hybrid wind-solarphotovoltaic(PV)syste...This research pioneers the integration of geographic information systems(GIS)and 3D modeling within a virtual reality(VR)framework to assess the viability and planning of a 20 MW hybrid wind-solarphotovoltaic(PV)system connected to the local grid.The study focuses on Dakhla,Morocco,a region with vast untapped renewable energy potential.By leveraging GIS,we are innovatively analyzing geographical and environmental factors that influence optimal site selection and system design.The incorporation of VR technologies offers an unprecedented level of realism and immersion,allowing stakeholders to virtually experience the project's impact and design in a dynamic,interactive environment.This novel methodology includes extensive data collection,advanced modeling,and simulations,ensuring that the hybrid system is precisely tailored to the unique climatic and environmental conditions of Dakhla.Our analysis reveals that the region possesses a photovoltaic solar potential of approximately2400 k Wh/m^(2) per year,with an average annual wind power density of about 434 W/m^(2) at an 80-meter hub height.Productivity simulations indicate that the 20 MW hybrid system could generate approximately 60 GWh of energy per year and 1369 GWh over its 25-year lifespan.To validate these findings,we employed the System Advisor Model(SAM)software and the Global Solar Photovoltaic Atlas platform.This comprehensive and interdisciplinary approach not only provides a robust assessment of the system's feasibility but also offers valuable insights into its potential socio-economic and environmental impact.展开更多
Directed degradation of abundant renewable lignin into small aromatic compounds is crucial for lignin valorization but challenging.The degradation of lignin in natural environments typically involves multienzyme syner...Directed degradation of abundant renewable lignin into small aromatic compounds is crucial for lignin valorization but challenging.The degradation of lignin in natural environments typically involves multienzyme synergy.However,the proteinaceous characteristics of lignin-degrading enzymes restrict their accessibility to certain regions of intricate lignin,resulting in the multienzyme systems being unable to fully demonstrate their effectiveness.Herein,a de novo biomimetic enzyme-nanozyme hybrid system was constructed by combiningλ-MnO_(2) nanozyme with laccase CotA from Bacillus subtilis,aimed at facilitating lignin degradation under mild conditions.The lignin degradation rate of the CotA+λ-MnO_(2) hybrid system was determined to be 25.15%,which was much higher than those of the lignin degradation systems with only laccase CotA(15.32%)orλ-MnO_(2) nanozyme(14.90%).Notably,the proportion of aromatic chemicals in the products derived from the hybrid system reached as much as 48%,which was 41.2%and 118.2%higher than those of the CotA-andλ-MnO_(2)-catalyzed systems,respectively.Analysis of products mapping and lignin structure changes suggested that the higher proportion of aromatic compounds in the CotA+λ-MnO_(2)hybrid system was more likely to benefit from the laccase-mediated methoxylation.Moreover,electron paramagnetic resonance analysis indicated that the intensity and kind of free radicals such as·OH and·O_(2)^(-)are closely linked to the degradation rate and reaction type.This work is the inaugural application of an enzyme-nanozyme hybrid system for lignin degradation,demonstrating the potential of the synergistic interaction between enzyme and nanozyme in the directed degradation of lignin.展开更多
In order to model effectively hybrid systems,a new modeling method of extended Petri nets,which is called extended object-orient hybrid Petri net （EOHPN）,is proposed.To deal with the complexity of hybrid systems, ob...In order to model effectively hybrid systems,a new modeling method of extended Petri nets,which is called extended object-orient hybrid Petri net （EOHPN）,is proposed.To deal with the complexity of hybrid systems, object-oriented abstraction mechanisms such as encapsulation and classifications are merged into EOHPN models.To combine the continuous part and discrete part of hybrid systems and to reduce the complexity of hybrid systems,a hybrid Petri net is introduced and extended with object-oriented modeling technology.Development of object models is suggested on the basis of the defined EOHPN.Finally, an application-oriented case is presented to illustrate that how the proposed EOHPN is used to model hybrid systems.The resulting model validates that the EOHPNs can deal with the modeling complexity of hybrid systems.展开更多
Abiotic-biological hybrid systems that combine the advantages of abiotic catalysis and biotransformation for the conversion of carbon dioxide(CO2)to value-added chemicals and fuels have emerged as an appealing way to ...Abiotic-biological hybrid systems that combine the advantages of abiotic catalysis and biotransformation for the conversion of carbon dioxide(CO2)to value-added chemicals and fuels have emerged as an appealing way to address the global energy and environmental crisis caused by increased CO2 emission.We illustrate the recent progress in this field.Here,we first review the natural CO2 fixation pathways for an in-depth understanding of the biological CO2 transformation strategy and why a sustainable feed of reducing power is important.Second,we review the recent progress in the construction of abiotic-biological hybrid systems for CO2 transformation from two aspects:(i)microbial electrosynthesis systems that utilize electricity to support whole-cell biological CO2 conversion to products of interest and(ii)photosynthetic semiconductor biohybrid systems that integrate semiconductor nanomaterials with CO2-fixing microorganisms to harness solar energy for biological CO2 transformation.Lastly,we discuss potential approaches for further improvement of abiotic-biological hybrid systems.展开更多
A single intersection of two phases is selected as a model to put forward a new optimal time-planning scheme for traffic light based on the model of hybrid automata for single intersection. A method of optimization is...A single intersection of two phases is selected as a model to put forward a new optimal time-planning scheme for traffic light based on the model of hybrid automata for single intersection. A method of optimization is proposed for hybrid systems, and the average queue length over all queues is used as an objective function to find an optimal switching scheme for traffic light. It is illustrated that traffic light control for single intersection is a typical hybrid system, and the optimal planning-time scheme can be obtained using the optimal hybrid systems control based on the two stages method.展开更多
The yeast two\|hybrid system is a molecular genetic approach for protein interaction and it is widely used to screen for proteins that interact with a protein of interest in recent years.This process includes,construc...The yeast two\|hybrid system is a molecular genetic approach for protein interaction and it is widely used to screen for proteins that interact with a protein of interest in recent years.This process includes,construction and testing of the bait plasmid,screening a plasmid library for interacting fusion protein,elimination of false positives and delection analysis of true positives.This procedure is designed to allow investigators to identify proteins and their encoding cDNAs that have a biologically significant interaction with a protein of interest.More and more studies have demonstrated that the two\|hybrid system is a powerful and sensitive technique for the identification of genes that code for proteins that interact in a biologically significant fashion with a protein of interest in higher plants.This method has been used to identify new interaction protein in many laboratories.The recently reported yeast tri\|brid system,should allow the investigation of more complex protein\|protein interactions.The aim of this review is to outline the recent progress made in protein interactions by using yeast two\|hybrid system.展开更多
The hybrid system with radiant cooling and dedicated outdoor air not only possesses high energy efficiency, but also creates a healthy and comfortable indoor environment. Indoor air quality will be improved by the ded...The hybrid system with radiant cooling and dedicated outdoor air not only possesses high energy efficiency, but also creates a healthy and comfortable indoor environment. Indoor air quality will be improved by the dedicated outdoor air system(DOAS) and indoor thermal comfort can be enhanced by the radiant cooling system(RCS). The optimal air-supply mode of the hybrid system and the corresponding design approach were investigated. A full-scale experimental chamber with various air outlets and the ceiling radiant cooling panels(CRCP) was designed and established. The performances of different air-supply modes along with CRCPs were analyzed by multi-index evaluations. Preliminary investigations were also conducted on the humidity stratification and the control effect of different airflow modes to prevent condensation on CRCP. The overhead supply air is recommended as the best combination mode for the hybrid system after comprehensive comparison of the experiment results. The optimal proportion of CRCP accounting for the total cooling capacities in accord with specific cooling loads is found, which may provide valuable reference for the design and operation of the hybrid system.展开更多
In this article, state feedback predictive controller for hybrid system via parametric programming is proposed. First, mixed logic dynamic （MLD） modeling mechanism for hybrid system is analyzed, which has a distingu...In this article, state feedback predictive controller for hybrid system via parametric programming is proposed. First, mixed logic dynamic （MLD） modeling mechanism for hybrid system is analyzed, which has a distinguished advantage to deal with the logic rules and constraints of a plant. Model predictive control algorithm with moving horizon state estimator （MHE） is presented. The estimator is adopted to estimate the current state of the plant with process disturbance and measurement noise, and the state estimated are utilized in the predictive controller for both regulation and tracking problems of the hybrid system based on MLD model. Off-line parametric programming is adopted and then on-line mixed integer programming problem can be treated as the parameter programming with estimated state as the parameters. A three tank system is used for computer simulation, results show that the proposed MHE based predictive control via parametric programming is effective for hybrid system with model/olant mismatch, and has a potential for the engineering applications.展开更多
For a class of non-uniform output sampling hybrid system with actuator faults and bounded disturbances,an iterative learning fault diagnosis algorithm is proposed.Firstly,in order to measure the impact of fault on sys...For a class of non-uniform output sampling hybrid system with actuator faults and bounded disturbances,an iterative learning fault diagnosis algorithm is proposed.Firstly,in order to measure the impact of fault on system between every consecutive output sampling instants,the actual fault function is transformed to obtain an equivalent fault model by using the integral mean value theorem,then the non-uniform sampling hybrid system is converted to continuous systems with timevarying delay based on the output delay method.Afterwards,an observer-based fault diagnosis filter with virtual fault is designed to estimate the equivalent fault,and the iterative learning regulation algorithm is chosen to update the virtual fault repeatedly to make it approximate the actual equivalent fault after some iterative learning trials,so the algorithm can detect and estimate the system faults adaptively.Simulation results of an electro-mechanical control system model with different types of faults illustrate the feasibility and effectiveness of this algorithm.展开更多
Modeling and simulation have emerged as an indispensable approach to create numerical experiment platforms and study engineering systems.However,the increasingly complicated systems that engineers face today dramatica...Modeling and simulation have emerged as an indispensable approach to create numerical experiment platforms and study engineering systems.However,the increasingly complicated systems that engineers face today dramatically challenge state-of-the-art modeling and simulation approaches.Such complicated systems,which are composed of not only continuous states but also discrete events,and which contain complex dynamics across multiple timescales,are defined as generalized hybrid systems(GHSs)in this paper.As a representative GHS,megawatt power electronics(MPE)systems have been largely integrated into the modern power grid,but MPE simulation remains a bottleneck due to its unacceptable time cost and poor convergence.To address this challenge,this paper proposes the numerical convex lens approach to achieve state-discretized modeling and simulation of GHSs.This approach transforms conventional time-discretized passive simulations designed for pure-continuous systems into state-discretized selective simulations designed for GHSs.When this approach was applied to a largescale MPE-based renewable energy system,a 1000-fold increase in simulation speed was achieved,in comparison with existing software.Furthermore,the proposed approach uniquely enables the switching transient simulation of a largescale megawatt system with high accuracy,compared with experimental results,and with no convergence concerns.The numerical convex lens approach leads to the highly efficient simulation of intricate GHSs across multiple timescales,and thus significantly extends engineers’capability to study systems with numerical experiments.展开更多
Under Type-Ⅱ progressively hybrid censoring, this paper discusses statistical inference and optimal design on stepstress partially accelerated life test for hybrid system in presence of masked data. It is assumed tha...Under Type-Ⅱ progressively hybrid censoring, this paper discusses statistical inference and optimal design on stepstress partially accelerated life test for hybrid system in presence of masked data. It is assumed that the lifetime of the component in hybrid systems follows independent and identical modified Weibull distributions. The maximum likelihood estimations(MLEs)of the unknown parameters, acceleration factor and reliability indexes are derived by using the Newton-Raphson algorithm. The asymptotic variance-covariance matrix and the approximate confidence intervals are obtained based on normal approximation to the asymptotic distribution of MLEs of model parameters. Moreover,two bootstrap confidence intervals are constructed by using the parametric bootstrap method. The optimal time of changing stress levels is determined under D-optimality and A-optimality criteria.Finally, the Monte Carlo simulation study is carried out to illustrate the proposed procedures.展开更多
In this paper, a decentralized proportional-derivative （PD） controller design for non-uniform motion of a Hamiltonian hybrid system is considered. A Hamiltonian hybrid system with the capability of producing a non-u...In this paper, a decentralized proportional-derivative （PD） controller design for non-uniform motion of a Hamiltonian hybrid system is considered. A Hamiltonian hybrid system with the capability of producing a non-uniform motion is developed. The structural properties of the system are investigated by means of the theory of Hamiltonian systems. A relationship between the parameters of the system and the parameters of the proposed decentralized PD controller is shown to ensure local stability and tracking performance. Simulation results are included to show the obtained non-uniform motion.展开更多
Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes.Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C vir...Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes.Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus(HCV)by cloning the gene of NS5ABP37 protein into pGBKT7,then the recombinant plasmid DNA was transformed into yeast AH109(α type).The transformed yeast AH109 was mated with yeast Y187(α type)containing liver cDNA library plasmid in 2×YPDA medium.Diploid yeast was plated on synthetic dropout nutrient medium(SD/-Trp-Leu-His-Ade)containing X-α-gal for selection and screening.After extracting and sequencing of plasmids from positive(blue)colonies,we made a sequence analysis by bioinformatics.Results We screened twenty-five proteins binding to NS5ABP37,including Homo sapiens cyclin I(CCNI)gene,Homo sapiens matrix metallopeptidase 25(MMP25)and Homo sapiens talin 1.Conclusion The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of HCV.And the biological function of NS5ABP37 may be associated with glycometabolism,lipid metabolism and apoptosis.展开更多
Combining wave energy converters(WECs)with floating offshore wind turbines proves a potential strategy to achieve better use of marine renewable energy.The full coupling investigation on the dynamic and power generati...Combining wave energy converters(WECs)with floating offshore wind turbines proves a potential strategy to achieve better use of marine renewable energy.The full coupling investigation on the dynamic and power generation features of the hybrid systems under operational sea states is necessary but limited by numerical simulation tools.Here an aero-hydro-servo-elastic coupling numerical tool is developed and applied to investigate the motion,mooring tension,and energy conversion performance of a hybrid system consisting of a spar-type floating wind turbine and an annular wave energy converter.Results show that the addition of the WEC has no significant negative effect on the dynamic performance of the platform and even enhances the rotational stability of the platform.For surge and pitch motion,the peak of the spectra is originated from the dominating wave component,whereas for the heave motion,the peak of the spectrum is the superposed effect of the dominating wave component and the resonance of the system.The addition of the annular WEC can slightly improve the wind power by making the rotor to be in a better position to face the incoming wind and provide considerable wave energy production,which can compensate for the downtime of the offshore wind.展开更多
The control objective and several key parameters of PEMFC hybrid system are analyzed. Control strategy design and energy optimization simulation are made individually for given cycle case and realtime operating case. ...The control objective and several key parameters of PEMFC hybrid system are analyzed. Control strategy design and energy optimization simulation are made individually for given cycle case and realtime operating case. For the given cycle case, genetic algorithm is adopted to solve the multi-constraint combinatorial optimization problem. Simulation result showed the algorithm＇s feasibility. As far as the realtime operation is concerned, based on the original fuzzy control strategy, the fuel cell voltage and voltage variance parameters are introduced to apply result reveals that the improved fuzzy control strategy can enhance the two-level modification on the fuzzy control output. The fuel cell efficiency and reduce the power fluctuations.展开更多
AIM: To investigate the biological function of complete S protein and to look for proteins interacting with complete S protein in hepatocytes. METHODS: We constructed bait plasmid expressing complete S protein of HBV ...AIM: To investigate the biological function of complete S protein and to look for proteins interacting with complete S protein in hepatocytes. METHODS: We constructed bait plasmid expressing complete S protein of HBV by cloning the gene of complete S protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (a type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we underwent sequence analysis by bioinformatics. RESULTS: Nineteen colonies were selected and sequenced. Among them, five colonies were Homo sapiens solute carrier family 25, member 23 (SLC25A23), one was Homo sapiens calrer.iculin, one was human serum albumin (ALB) gene, one was Homo sapiens metallothionein 2A, two were Homo sapiens betaine-homocysteine methyltransferase, three were Homo sapiens Na+ and H+coupled amino acid transport system N, one was Homo sapiens CD81 antigen (target of anti-proliferative antibody 1) (CD81), three were Homo sapiens diazepam binding inhibitor, two colonies were new genes with unknown function. CONCLUSION: The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with complete S protein of HBV. The complete S protein may bind to different proteins i.e., its multiple functions in vivo.展开更多
In a hybrid system, the subsystems with discrete dynamics play a central role in a hybrid system. In the course of engineering machinery of cluster construction, the discrete control law is hard to obtain because the ...In a hybrid system, the subsystems with discrete dynamics play a central role in a hybrid system. In the course of engineering machinery of cluster construction, the discrete control law is hard to obtain because the construction environment is complex and there exist many affecting factors. In this paper, hierarchically intelligent control, expert control and fuzzy control are introduced into the discrete subsystems of engineering machinery of cluster hybrid system, so as to rebuild the hybrid system and make the discrete control law easily and effectively obtained. The structures, reasoning mechanism and arithmetic of intelligent control are replanted to discrete dynamic, conti- nuous process and the interface of the hybrid system. The structures of three types of intelligent hybrid system are presented and the human experiences summarized from engineering machinery of cluster are taken into account.展开更多
Practical stabilities for linear fractional impulsive hybrid systems are investigated in detail.The transformation from a linear fractional differential system to a fractional impulsive hybrid system is interpreted.Wi...Practical stabilities for linear fractional impulsive hybrid systems are investigated in detail.The transformation from a linear fractional differential system to a fractional impulsive hybrid system is interpreted.With the help of the Mittag-Leffler functions for matrix-type,several practical stability criteria for fractional impulsive hybrid systems are derived.Finally,a numerical example is provided to illustrate the effectiveness of the results.展开更多
基金supported by the National Natural Science Foundation of China(61833005)
文摘Dear Editor,This letter studies finite-time stability (FTS) of impulsive and switched hybrid systems with delay-dependent impulses. Some conditions, based on Lyapunov method, are proposed for ensuring FTS and estimating settling-time function (STF) of the hybrid systems.When switching dynamics are FTS and impulsive dynamics involve destabilizing delay-dependent impulses, the FTS is retained if the impulses occur infrequently.
基金supported by National Natural Science Foundation of China(62263014)Yunnan Provincial Basic Research Project(202401AT070344,202301AT070443).
文摘This study integrates the individual photovoltaic(PV)and thermoelectric generator(TEG)systems into a PV-TEG hybrid system to improve its overall power output by reutilizing the waste heat generated during PV power production to enhance its operational relia-bility.However,stochastic environmental conditions often result in partial shading conditions and nonuniform thermal distribution across the PV-TEG modules,which negatively affect the output characteristics of the system,thus presenting a significant challenge to maintaining their optimal performance.To address these challenges,a novel fitness-distance-balance-based beluga whale optimization(FDBBWO)strategy has been devised for maximizing the power output of the PV-TEG hybrid system under dynamic operation scenar-ios.A broader spectrum of complex and authentic operational contexts has been considered in case studies to examine the effectiveness and feasibility of FDBBWO.For this,real-world datasets collected from different seasons in Hong Kong have been used to validate the practical viability of the proposed strategy.Simulation results reveal that the FDBBWO based maximum power point tracking technique outperforms its competing methods by achieving the highest energy output,with a remarkable increase of up to 134.25%with minimal power fluctuations.For instance,the energy obtained by FDBBWO is 47.45%and 58.34%higher than BWO and perturb and observe methods,respectively,in the winter season.
文摘This research pioneers the integration of geographic information systems(GIS)and 3D modeling within a virtual reality(VR)framework to assess the viability and planning of a 20 MW hybrid wind-solarphotovoltaic(PV)system connected to the local grid.The study focuses on Dakhla,Morocco,a region with vast untapped renewable energy potential.By leveraging GIS,we are innovatively analyzing geographical and environmental factors that influence optimal site selection and system design.The incorporation of VR technologies offers an unprecedented level of realism and immersion,allowing stakeholders to virtually experience the project's impact and design in a dynamic,interactive environment.This novel methodology includes extensive data collection,advanced modeling,and simulations,ensuring that the hybrid system is precisely tailored to the unique climatic and environmental conditions of Dakhla.Our analysis reveals that the region possesses a photovoltaic solar potential of approximately2400 k Wh/m^(2) per year,with an average annual wind power density of about 434 W/m^(2) at an 80-meter hub height.Productivity simulations indicate that the 20 MW hybrid system could generate approximately 60 GWh of energy per year and 1369 GWh over its 25-year lifespan.To validate these findings,we employed the System Advisor Model(SAM)software and the Global Solar Photovoltaic Atlas platform.This comprehensive and interdisciplinary approach not only provides a robust assessment of the system's feasibility but also offers valuable insights into its potential socio-economic and environmental impact.
文摘Directed degradation of abundant renewable lignin into small aromatic compounds is crucial for lignin valorization but challenging.The degradation of lignin in natural environments typically involves multienzyme synergy.However,the proteinaceous characteristics of lignin-degrading enzymes restrict their accessibility to certain regions of intricate lignin,resulting in the multienzyme systems being unable to fully demonstrate their effectiveness.Herein,a de novo biomimetic enzyme-nanozyme hybrid system was constructed by combiningλ-MnO_(2) nanozyme with laccase CotA from Bacillus subtilis,aimed at facilitating lignin degradation under mild conditions.The lignin degradation rate of the CotA+λ-MnO_(2) hybrid system was determined to be 25.15%,which was much higher than those of the lignin degradation systems with only laccase CotA(15.32%)orλ-MnO_(2) nanozyme(14.90%).Notably,the proportion of aromatic chemicals in the products derived from the hybrid system reached as much as 48%,which was 41.2%and 118.2%higher than those of the CotA-andλ-MnO_(2)-catalyzed systems,respectively.Analysis of products mapping and lignin structure changes suggested that the higher proportion of aromatic compounds in the CotA+λ-MnO_(2)hybrid system was more likely to benefit from the laccase-mediated methoxylation.Moreover,electron paramagnetic resonance analysis indicated that the intensity and kind of free radicals such as·OH and·O_(2)^(-)are closely linked to the degradation rate and reaction type.This work is the inaugural application of an enzyme-nanozyme hybrid system for lignin degradation,demonstrating the potential of the synergistic interaction between enzyme and nanozyme in the directed degradation of lignin.
基金The National Key Laboratory Program ( No.51458060104JW0316)the National High Technology Research and De-velopment Program of China (863 Program) (No.2003AA414120).
文摘In order to model effectively hybrid systems,a new modeling method of extended Petri nets,which is called extended object-orient hybrid Petri net （EOHPN）,is proposed.To deal with the complexity of hybrid systems, object-oriented abstraction mechanisms such as encapsulation and classifications are merged into EOHPN models.To combine the continuous part and discrete part of hybrid systems and to reduce the complexity of hybrid systems,a hybrid Petri net is introduced and extended with object-oriented modeling technology.Development of object models is suggested on the basis of the defined EOHPN.Finally, an application-oriented case is presented to illustrate that how the proposed EOHPN is used to model hybrid systems.The resulting model validates that the EOHPNs can deal with the modeling complexity of hybrid systems.
文摘Abiotic-biological hybrid systems that combine the advantages of abiotic catalysis and biotransformation for the conversion of carbon dioxide(CO2)to value-added chemicals and fuels have emerged as an appealing way to address the global energy and environmental crisis caused by increased CO2 emission.We illustrate the recent progress in this field.Here,we first review the natural CO2 fixation pathways for an in-depth understanding of the biological CO2 transformation strategy and why a sustainable feed of reducing power is important.Second,we review the recent progress in the construction of abiotic-biological hybrid systems for CO2 transformation from two aspects:(i)microbial electrosynthesis systems that utilize electricity to support whole-cell biological CO2 conversion to products of interest and(ii)photosynthetic semiconductor biohybrid systems that integrate semiconductor nanomaterials with CO2-fixing microorganisms to harness solar energy for biological CO2 transformation.Lastly,we discuss potential approaches for further improvement of abiotic-biological hybrid systems.
文摘A single intersection of two phases is selected as a model to put forward a new optimal time-planning scheme for traffic light based on the model of hybrid automata for single intersection. A method of optimization is proposed for hybrid systems, and the average queue length over all queues is used as an objective function to find an optimal switching scheme for traffic light. It is illustrated that traffic light control for single intersection is a typical hybrid system, and the optimal planning-time scheme can be obtained using the optimal hybrid systems control based on the two stages method.
文摘The yeast two\|hybrid system is a molecular genetic approach for protein interaction and it is widely used to screen for proteins that interact with a protein of interest in recent years.This process includes,construction and testing of the bait plasmid,screening a plasmid library for interacting fusion protein,elimination of false positives and delection analysis of true positives.This procedure is designed to allow investigators to identify proteins and their encoding cDNAs that have a biologically significant interaction with a protein of interest.More and more studies have demonstrated that the two\|hybrid system is a powerful and sensitive technique for the identification of genes that code for proteins that interact in a biologically significant fashion with a protein of interest in higher plants.This method has been used to identify new interaction protein in many laboratories.The recently reported yeast tri\|brid system,should allow the investigation of more complex protein\|protein interactions.The aim of this review is to outline the recent progress made in protein interactions by using yeast two\|hybrid system.
基金Project(51178298)supported by the National Natural Science Foundation of China
文摘The hybrid system with radiant cooling and dedicated outdoor air not only possesses high energy efficiency, but also creates a healthy and comfortable indoor environment. Indoor air quality will be improved by the dedicated outdoor air system(DOAS) and indoor thermal comfort can be enhanced by the radiant cooling system(RCS). The optimal air-supply mode of the hybrid system and the corresponding design approach were investigated. A full-scale experimental chamber with various air outlets and the ceiling radiant cooling panels(CRCP) was designed and established. The performances of different air-supply modes along with CRCPs were analyzed by multi-index evaluations. Preliminary investigations were also conducted on the humidity stratification and the control effect of different airflow modes to prevent condensation on CRCP. The overhead supply air is recommended as the best combination mode for the hybrid system after comprehensive comparison of the experiment results. The optimal proportion of CRCP accounting for the total cooling capacities in accord with specific cooling loads is found, which may provide valuable reference for the design and operation of the hybrid system.
文摘In this article, state feedback predictive controller for hybrid system via parametric programming is proposed. First, mixed logic dynamic （MLD） modeling mechanism for hybrid system is analyzed, which has a distinguished advantage to deal with the logic rules and constraints of a plant. Model predictive control algorithm with moving horizon state estimator （MHE） is presented. The estimator is adopted to estimate the current state of the plant with process disturbance and measurement noise, and the state estimated are utilized in the predictive controller for both regulation and tracking problems of the hybrid system based on MLD model. Off-line parametric programming is adopted and then on-line mixed integer programming problem can be treated as the parameter programming with estimated state as the parameters. A three tank system is used for computer simulation, results show that the proposed MHE based predictive control via parametric programming is effective for hybrid system with model/olant mismatch, and has a potential for the engineering applications.
基金supported by the National Natural Science Foundation of China(61273070,61203092)the Enterprise-college-institute Cooperative Project of Jiangsu Province(BY2015019-21)+1 种基金111 Project(B12018)the Fun-damental Research Funds for the Central Universities(JUSRP51733B)
文摘For a class of non-uniform output sampling hybrid system with actuator faults and bounded disturbances,an iterative learning fault diagnosis algorithm is proposed.Firstly,in order to measure the impact of fault on system between every consecutive output sampling instants,the actual fault function is transformed to obtain an equivalent fault model by using the integral mean value theorem,then the non-uniform sampling hybrid system is converted to continuous systems with timevarying delay based on the output delay method.Afterwards,an observer-based fault diagnosis filter with virtual fault is designed to estimate the equivalent fault,and the iterative learning regulation algorithm is chosen to update the virtual fault repeatedly to make it approximate the actual equivalent fault after some iterative learning trials,so the algorithm can detect and estimate the system faults adaptively.Simulation results of an electro-mechanical control system model with different types of faults illustrate the feasibility and effectiveness of this algorithm.
基金the Major Program of National Natural Science Foundation of China(51490683).
文摘Modeling and simulation have emerged as an indispensable approach to create numerical experiment platforms and study engineering systems.However,the increasingly complicated systems that engineers face today dramatically challenge state-of-the-art modeling and simulation approaches.Such complicated systems,which are composed of not only continuous states but also discrete events,and which contain complex dynamics across multiple timescales,are defined as generalized hybrid systems(GHSs)in this paper.As a representative GHS,megawatt power electronics(MPE)systems have been largely integrated into the modern power grid,but MPE simulation remains a bottleneck due to its unacceptable time cost and poor convergence.To address this challenge,this paper proposes the numerical convex lens approach to achieve state-discretized modeling and simulation of GHSs.This approach transforms conventional time-discretized passive simulations designed for pure-continuous systems into state-discretized selective simulations designed for GHSs.When this approach was applied to a largescale MPE-based renewable energy system,a 1000-fold increase in simulation speed was achieved,in comparison with existing software.Furthermore,the proposed approach uniquely enables the switching transient simulation of a largescale megawatt system with high accuracy,compared with experimental results,and with no convergence concerns.The numerical convex lens approach leads to the highly efficient simulation of intricate GHSs across multiple timescales,and thus significantly extends engineers’capability to study systems with numerical experiments.
基金supported by the National Natural Science Foundation of China(71401134 71571144+1 种基金 71171164)the Program of International Cooperation and Exchanges in Science and Technology Funded by Shaanxi Province(2016KW-033)
文摘Under Type-Ⅱ progressively hybrid censoring, this paper discusses statistical inference and optimal design on stepstress partially accelerated life test for hybrid system in presence of masked data. It is assumed that the lifetime of the component in hybrid systems follows independent and identical modified Weibull distributions. The maximum likelihood estimations(MLEs)of the unknown parameters, acceleration factor and reliability indexes are derived by using the Newton-Raphson algorithm. The asymptotic variance-covariance matrix and the approximate confidence intervals are obtained based on normal approximation to the asymptotic distribution of MLEs of model parameters. Moreover,two bootstrap confidence intervals are constructed by using the parametric bootstrap method. The optimal time of changing stress levels is determined under D-optimality and A-optimality criteria.Finally, the Monte Carlo simulation study is carried out to illustrate the proposed procedures.
文摘In this paper, a decentralized proportional-derivative （PD） controller design for non-uniform motion of a Hamiltonian hybrid system is considered. A Hamiltonian hybrid system with the capability of producing a non-uniform motion is developed. The structural properties of the system are investigated by means of the theory of Hamiltonian systems. A relationship between the parameters of the system and the parameters of the proposed decentralized PD controller is shown to ensure local stability and tracking performance. Simulation results are included to show the obtained non-uniform motion.
文摘Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes.Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus(HCV)by cloning the gene of NS5ABP37 protein into pGBKT7,then the recombinant plasmid DNA was transformed into yeast AH109(α type).The transformed yeast AH109 was mated with yeast Y187(α type)containing liver cDNA library plasmid in 2×YPDA medium.Diploid yeast was plated on synthetic dropout nutrient medium(SD/-Trp-Leu-His-Ade)containing X-α-gal for selection and screening.After extracting and sequencing of plasmids from positive(blue)colonies,we made a sequence analysis by bioinformatics.Results We screened twenty-five proteins binding to NS5ABP37,including Homo sapiens cyclin I(CCNI)gene,Homo sapiens matrix metallopeptidase 25(MMP25)and Homo sapiens talin 1.Conclusion The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of HCV.And the biological function of NS5ABP37 may be associated with glycometabolism,lipid metabolism and apoptosis.
基金financially supported by the Key-Area Research and Development Program of Guangdong Province (Grant No.2020B1111010001)the National Natural Science Foundation of China (Grant Nos.52071096 and 52201322)+3 种基金the National Natural Science Foundation of China National Outstanding Youth Science Fund Project (Grant No.52222109)Guangdong Basic and Applied Basic Research Foundation (Grant No.2022B1515020036)the Fundamental Research Funds for the Central Universities (Grant No.2022ZYGXZR014)the State Key Laboratory of Coastal and Offshore Engineering through the Open Research Fund Program (Grant No.LP2214)。
文摘Combining wave energy converters(WECs)with floating offshore wind turbines proves a potential strategy to achieve better use of marine renewable energy.The full coupling investigation on the dynamic and power generation features of the hybrid systems under operational sea states is necessary but limited by numerical simulation tools.Here an aero-hydro-servo-elastic coupling numerical tool is developed and applied to investigate the motion,mooring tension,and energy conversion performance of a hybrid system consisting of a spar-type floating wind turbine and an annular wave energy converter.Results show that the addition of the WEC has no significant negative effect on the dynamic performance of the platform and even enhances the rotational stability of the platform.For surge and pitch motion,the peak of the spectra is originated from the dominating wave component,whereas for the heave motion,the peak of the spectrum is the superposed effect of the dominating wave component and the resonance of the system.The addition of the annular WEC can slightly improve the wind power by making the rotor to be in a better position to face the incoming wind and provide considerable wave energy production,which can compensate for the downtime of the offshore wind.
基金Project (No. 2003AA517020) supported by the Hi-Tech Researchand Development Program (863) of China
文摘The control objective and several key parameters of PEMFC hybrid system are analyzed. Control strategy design and energy optimization simulation are made individually for given cycle case and realtime operating case. For the given cycle case, genetic algorithm is adopted to solve the multi-constraint combinatorial optimization problem. Simulation result showed the algorithm＇s feasibility. As far as the realtime operation is concerned, based on the original fuzzy control strategy, the fuel cell voltage and voltage variance parameters are introduced to apply result reveals that the improved fuzzy control strategy can enhance the two-level modification on the fuzzy control output. The fuel cell efficiency and reduce the power fluctuations.
基金Supported by the National Natural Science Foundation of China, No. C03011402, No. C30070690the Science and Technique Foundation of PLA during the 9th Five-year plan period, No. 98D063 the Launching Foundation for Students Studying Abroad of PLA, No. 98H038 the Youth Science and Technique Foundation of PLA during the 10lh Five-year plan period, No. 01Q138and the Science & Technique Foundation of PLA during the 10th Five-year plan period, No. 01MB135
文摘AIM: To investigate the biological function of complete S protein and to look for proteins interacting with complete S protein in hepatocytes. METHODS: We constructed bait plasmid expressing complete S protein of HBV by cloning the gene of complete S protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (a type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we underwent sequence analysis by bioinformatics. RESULTS: Nineteen colonies were selected and sequenced. Among them, five colonies were Homo sapiens solute carrier family 25, member 23 (SLC25A23), one was Homo sapiens calrer.iculin, one was human serum albumin (ALB) gene, one was Homo sapiens metallothionein 2A, two were Homo sapiens betaine-homocysteine methyltransferase, three were Homo sapiens Na+ and H+coupled amino acid transport system N, one was Homo sapiens CD81 antigen (target of anti-proliferative antibody 1) (CD81), three were Homo sapiens diazepam binding inhibitor, two colonies were new genes with unknown function. CONCLUSION: The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with complete S protein of HBV. The complete S protein may bind to different proteins i.e., its multiple functions in vivo.
文摘In a hybrid system, the subsystems with discrete dynamics play a central role in a hybrid system. In the course of engineering machinery of cluster construction, the discrete control law is hard to obtain because the construction environment is complex and there exist many affecting factors. In this paper, hierarchically intelligent control, expert control and fuzzy control are introduced into the discrete subsystems of engineering machinery of cluster hybrid system, so as to rebuild the hybrid system and make the discrete control law easily and effectively obtained. The structures, reasoning mechanism and arithmetic of intelligent control are replanted to discrete dynamic, conti- nuous process and the interface of the hybrid system. The structures of three types of intelligent hybrid system are presented and the human experiences summarized from engineering machinery of cluster are taken into account.
基金Natural Science Foundation of Shanghai China (No. 10ZR1400100)
文摘Practical stabilities for linear fractional impulsive hybrid systems are investigated in detail.The transformation from a linear fractional differential system to a fractional impulsive hybrid system is interpreted.With the help of the Mittag-Leffler functions for matrix-type,several practical stability criteria for fractional impulsive hybrid systems are derived.Finally,a numerical example is provided to illustrate the effectiveness of the results.
