SAUR(SMALL AUXIN UP RNA)基因家族是促进下胚轴伸长的重要下游基因,然而在高温诱导的下胚轴伸长中SAURs受调控的分子机制尚有很多不明之处。热形态建成被定义为高等植物在最适温度和逆境高温之间的温和高温范围内发生的一系列形态变化...SAUR(SMALL AUXIN UP RNA)基因家族是促进下胚轴伸长的重要下游基因,然而在高温诱导的下胚轴伸长中SAURs受调控的分子机制尚有很多不明之处。热形态建成被定义为高等植物在最适温度和逆境高温之间的温和高温范围内发生的一系列形态变化。其中,高温诱导的下胚轴伸长是研究最为透彻的一个。该研究以拟南芥Col野生型、hy5突变体、35 S::HY5-HA/Col过表达植物和烟草为材料,采用抑制剂NPA处理实验、定量RT-PCR、染色质免疫共沉淀和双荧光素酶报告基因检测方法探索了高温调控SAUR1/2/3/4的分子机制。结果表明:(1)热形态建成信号转导通路中生长素处于HY5(ELONGATED HYPOCOTYL 5)的下游。(2)在20℃和29℃中,HY5抑制了SAUR1/2/3/4的转录。(3)在常温和高温中,HY5与SAUR1/2/3/4的启动子区染色质含有E-box的部分结合且这些结合被高温所抑制。(4)HY5对SAUR1/2/3/4的调控依然需要生长素的参与。综上认为,高温通过影响HY5与SAUR1/2/3/4启动子染色质的结合强度来调控这4个基因的转录,并且此调控过程需要生长素。该研究结果为高温调控下胚轴伸长下游基因的分子机制提供了新的见解。展开更多
With the globalization of the economy,maritime trade has surged,posing challenges in the supervision of marine vessel activities.An automatic identification system(AIS)is an effective means of shipping traffic service...With the globalization of the economy,maritime trade has surged,posing challenges in the supervision of marine vessel activities.An automatic identification system(AIS)is an effective means of shipping traffic service,but many uncertainties exist regarding its data quality.In this study,the AIS data from Haiyang(HY)series of satellites were used to assess the data quality,analyze the global ship trajectory distribution and update frequencies from 2019 to 2023.Through the analysis of maritime mobile service identity numbers,we identified 340185 unique vessels,80.1%of which adhered to the International Telecommunication Union standards.Approximately 49.7%of ships exhibit significant data gaps,and 1.1%show inconsistencies in their AIS data sources.In the central Pacific Ocean at low latitudes and along the coast of South America(30°-60°S),a heightened incidence of abnormal trajectories of ships has been consistently observed,particularly in areas associated with fishing activities.According to the spatial distribution of ship trajectories,AIS data exhibit numerous deficiencies,particularly in high-traffic regions such as the East China Sea and South China Sea.In contrast,ship trajectories in the polar regions,characterized by high latitudes,are relatively comprehensive.With the increased number of HY satellites equipped with AIS receivers,the quantity of trajectory points displays a growing trend,leading to increasingly complete trajectories.This trend highlights the significant potential of using AIS data acquired from HY satellites to increase the accuracy of vessel tracking.展开更多
基金The National Key R&D Program of China under contract Nos 2021YFC2803305 and 2024YFC2816301the Fundamental Research Funds for the Central Universities of China under contract No.2042022dx0001.
文摘With the globalization of the economy,maritime trade has surged,posing challenges in the supervision of marine vessel activities.An automatic identification system(AIS)is an effective means of shipping traffic service,but many uncertainties exist regarding its data quality.In this study,the AIS data from Haiyang(HY)series of satellites were used to assess the data quality,analyze the global ship trajectory distribution and update frequencies from 2019 to 2023.Through the analysis of maritime mobile service identity numbers,we identified 340185 unique vessels,80.1%of which adhered to the International Telecommunication Union standards.Approximately 49.7%of ships exhibit significant data gaps,and 1.1%show inconsistencies in their AIS data sources.In the central Pacific Ocean at low latitudes and along the coast of South America(30°-60°S),a heightened incidence of abnormal trajectories of ships has been consistently observed,particularly in areas associated with fishing activities.According to the spatial distribution of ship trajectories,AIS data exhibit numerous deficiencies,particularly in high-traffic regions such as the East China Sea and South China Sea.In contrast,ship trajectories in the polar regions,characterized by high latitudes,are relatively comprehensive.With the increased number of HY satellites equipped with AIS receivers,the quantity of trajectory points displays a growing trend,leading to increasingly complete trajectories.This trend highlights the significant potential of using AIS data acquired from HY satellites to increase the accuracy of vessel tracking.
