Background and objectives:Gelsemium elegans Benth(G.elegans)is a traditional medicinal plant;however,it is highly toxic,and toxicity varies significantly between species.The cause of this difference has not been clari...Background and objectives:Gelsemium elegans Benth(G.elegans)is a traditional medicinal plant;however,it is highly toxic,and toxicity varies significantly between species.The cause of this difference has not been clarified.Humantenirine is an important toxic alkaloid in G.elegans,and its metabolism has been poorly studied.This study aimed to compare the different metabolites formed by human liver microsomes,pig liver microsomes,and goat liver microsomes.Methods:High-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used to study the metabolism of humantenirine in human liver microsomes,pig liver microsomes,and goat liver microsomes.Results:A total of eight metabolites(M1-M8)were identified,and three major metabolic pathways were found:demethylation(M1),dehydrogenation(M2,M3,M7),and oxidation(M4,M5,M6,M8).Conclusions:Based on these results,it is hypothesized that demethylation is the major detoxification pathway for humantenirine,providing important information to better understand the metabolism and toxicity differences between species of G.elegans.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.31972737).
文摘Background and objectives:Gelsemium elegans Benth(G.elegans)is a traditional medicinal plant;however,it is highly toxic,and toxicity varies significantly between species.The cause of this difference has not been clarified.Humantenirine is an important toxic alkaloid in G.elegans,and its metabolism has been poorly studied.This study aimed to compare the different metabolites formed by human liver microsomes,pig liver microsomes,and goat liver microsomes.Methods:High-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used to study the metabolism of humantenirine in human liver microsomes,pig liver microsomes,and goat liver microsomes.Results:A total of eight metabolites(M1-M8)were identified,and three major metabolic pathways were found:demethylation(M1),dehydrogenation(M2,M3,M7),and oxidation(M4,M5,M6,M8).Conclusions:Based on these results,it is hypothesized that demethylation is the major detoxification pathway for humantenirine,providing important information to better understand the metabolism and toxicity differences between species of G.elegans.
