Fibronectin(Fn)is a major molecular glycoprotein that is known to play an important role in many systems involved in cell-to-cell interation and cell-to-matrix adhesion.We initially studied the effect of Fn on the fer...Fibronectin(Fn)is a major molecular glycoprotein that is known to play an important role in many systems involved in cell-to-cell interation and cell-to-matrix adhesion.We initially studied the effect of Fn on the fertilization capacity Of human spermatozoa with a penetration test in vitro.Following co--incubation of Fn in a heterologous system(human spermatozoa and zona-free golden hamster oocytes),we have noted a significant decrease in the rate of sperm penetration into oolemma at 2.8μg Fn/ml,and a complete inhibition of fertilization at 15μg Fn/ml.Besides,we noted a specific increase in the penetration rate during the co-incubation of anti-Fn-serum in a heterologous system.These results suggest that extrinsic Fn binds with the Fn-receptor in the oolemma,thereby,competitively inhibiting fertilization.展开更多
文摘Fibronectin(Fn)is a major molecular glycoprotein that is known to play an important role in many systems involved in cell-to-cell interation and cell-to-matrix adhesion.We initially studied the effect of Fn on the fertilization capacity Of human spermatozoa with a penetration test in vitro.Following co--incubation of Fn in a heterologous system(human spermatozoa and zona-free golden hamster oocytes),we have noted a significant decrease in the rate of sperm penetration into oolemma at 2.8μg Fn/ml,and a complete inhibition of fertilization at 15μg Fn/ml.Besides,we noted a specific increase in the penetration rate during the co-incubation of anti-Fn-serum in a heterologous system.These results suggest that extrinsic Fn binds with the Fn-receptor in the oolemma,thereby,competitively inhibiting fertilization.
