Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical disability.Thus,in the search for more effective treatments than those already applied fo...Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical disability.Thus,in the search for more effective treatments than those already applied for years,which are not entirely efficient,researches have been able to demonstrate the potential of biological strategies using biomaterials to tissue manufacturing through bioengineering and stem cell therapy as a neuroregenerative approach,seeking to promote neuronal recovery after spinal cord injury.Each of these strategies has been developed and meticulously evaluated in several animal models with the aim of analyzing the potential of interventions for neuronal repair and,consequently,boosting functional recovery.Although the majority of experimental research has been conducted in rodents,there is increasing recognition of the importance,and need,of evaluating the safety and efficacy of these interventions in non-human primates before moving to clinical trials involving therapies potentially promising in humans.This article is a literature review from databases(PubMed,Science Direct,Elsevier,Scielo,Redalyc,Cochrane,and NCBI)from 10 years ago to date,using keywords(spinal cord injury,cell therapy,non-human primates,humans,and bioengineering in spinal cord injury).From 110 retrieved articles,after two selection rounds based on inclusion and exclusion criteria,21 articles were analyzed.Thus,this review arises from the need to recognize the experimental therapeutic advances applied in non-human primates and even humans,aimed at deepening these strategies and identifying the advantages and influence of the results on extrapolation for clinical applicability in humans.展开更多
As one of the latest research hotspots in the field of artificial intelligence,AI-generated content(AIGC)can create virtual teaching scenarios,such as those involving educational digital humans,through content creatio...As one of the latest research hotspots in the field of artificial intelligence,AI-generated content(AIGC)can create virtual teaching scenarios,such as those involving educational digital humans,through content creation.This integration of AI and education is a product that will profoundly change the form of online education and the learning experience.Currently,most courses for educational digital humans still rely on traditional educational models and face many problems in practice.Based on an overview of AIGC technology and its application in micro-course production,this paper analyzes the current status of micro-courses for educational digital humans.It proposes the application of AIGC technology and educational digital humans in micro-course production,namely,using AIGC technology to drive the transformation of micro-courses for educational digital humans towards intelligence,thereby improving education quality and efficiency.展开更多
诊断准确性试验(diagnostic test accuracy,DTA)的系统评价/Meta分析报告规范(PRISMA-DTA)用于规范和提高DTA的系统评价/Meta分析的报告质量,其于2018年1月发表在JAMA杂志。本文对其条目进行解读,以促进国内学者对PRISMA-DTA的理解和应...诊断准确性试验(diagnostic test accuracy,DTA)的系统评价/Meta分析报告规范(PRISMA-DTA)用于规范和提高DTA的系统评价/Meta分析的报告质量,其于2018年1月发表在JAMA杂志。本文对其条目进行解读,以促进国内学者对PRISMA-DTA的理解和应用,以期提高DTA的系统评价/Meta分析报告质量。展开更多
This research investigates the suitability of PRISMA and Sentinel-2 satellite imagery for retrieving topsoil properties such as Organic Matter(OM),Nitrogen(N),Phosphorus(P),Potassium(K),and pH in croplands using diffe...This research investigates the suitability of PRISMA and Sentinel-2 satellite imagery for retrieving topsoil properties such as Organic Matter(OM),Nitrogen(N),Phosphorus(P),Potassium(K),and pH in croplands using different Machine Learning(ML)algorithms and signal pre-treatments.Ninety-five soil samples were collected in Quzhou County,Northeast China.Satellite images captured soil reflectance data when bare soil was visible.For PRISMA data,a Linear Mixture Model(LMM)was used to separate soil and Photosynthetic Vegetation(PV)endmembers,excluding Non-Photosynthetic Vegetation(NPV)using Band Depth values at the 2100 nm absorption peak of cellulose.Sentinel-2 bare soil reflectance spectra were obtained using thresholds based on NDVI and NBR2 indices.Results showed PRISMA data provided slightly better accuracy in retrieving topsoil nutrients than Sentinel-2.While no optimal predictive algorithm was best,absorbance data proved more effective than reflectance.PRISMA results demonstrated potential for predicting soil nutrients in real scenarios.展开更多
Infertility represents a significant health concern,with sperm quantity and quality being crucial determinants of male fertility.Oligoasthenoteratozoospermia(OAT)is characterized by reduced sperm motility,lower sperm ...Infertility represents a significant health concern,with sperm quantity and quality being crucial determinants of male fertility.Oligoasthenoteratozoospermia(OAT)is characterized by reduced sperm motility,lower sperm concentration,and morphological abnormalities in sperm heads and flagella.Although variants in several genes have been implicated in OAT,its genetic etiologies and pathogenetic mechanisms remain inadequately understood.In this study,we identified a homozygous nonsense mutation(c.916C>T,p.Arg306*)in the coiled-coil domain containing 146(CCDC146)gene in an infertile male patient with OAT.This mutation resulted in the production of a truncated CCDC146 protein(amino acids 1-305),retaining only two out of five coiled-coil domains.To validate the pathogenicity of the CCDC146 mutation,we generated a mouse model(Ccdc146^(mut/mut))with a similar mutation to that of the patient.Consistently,the Ccdc146mut/mut mice exhibited infertility,characterized by significantly reduced sperm counts,diminished motility,and multiple defects in sperm heads and flagella.Furthermore,the levels of axonemal proteins,including DNAH17,DNAH1,and SPAG6,were significantly reduced in the sperm of Ccdc146^(mut/mut) mice.Additionally,both human and mouse CCDC146 interacted with intraflagellar transport protein 20(IFT20),but this interaction was lost in the mutated versions,leading to the degradation of IFT20.This study identified a novel deleterious homozygous nonsense mutation in CCDC146 that causes male infertility,potentially by disrupting axonemal protein transportation.These findings offer valuable insights for genetic counseling and understanding the mechanisms underlying CCDC146 mutant-associated infertility in human males.展开更多
网状Meta分析(network Meta-analysis,NMA)目前作为循证医学发展的二代方法学,越来越受到各国卫生技术评估组织的认可。然而,目前NMA的报告存在严重的问题。本文针对NMA的优先报告规范——PRISMA(preferred reporting items for systema...网状Meta分析(network Meta-analysis,NMA)目前作为循证医学发展的二代方法学,越来越受到各国卫生技术评估组织的认可。然而,目前NMA的报告存在严重的问题。本文针对NMA的优先报告规范——PRISMA(preferred reporting items for systematic reviews and meta analysis)扩展申明发表在Annals of Internal Medicine上,现将PRISMA扩展申明予以介绍,为国内研究人员撰写和报告高质量的NMA提供借鉴和参考。展开更多
目前普通系统评价/Meta分析都被要求遵循PRISMA(Preferred Reporting Items for Systematic Reviews and Meta-Analyses)进行规范报告。PRISMA harms清单是PRISMA报告规范的衍生版,其主要针对安全性系统评价如何进行报告提出条件要求。P...目前普通系统评价/Meta分析都被要求遵循PRISMA(Preferred Reporting Items for Systematic Reviews and Meta-Analyses)进行规范报告。PRISMA harms清单是PRISMA报告规范的衍生版,其主要针对安全性系统评价如何进行报告提出条件要求。PRISMA harms清单包括标题、摘要、前言、方法、结果、讨论和资助等需要报告的27个条目内容。该规范包括了清单、详尽的解释性文本以方便使用。通过应用PRISMA harms清单,作者、期刊编辑和同行评议者可促进安全性系统评价报告的质量。展开更多
目的应用Prisma声明评价活血化瘀法治疗中风的系统评价/Meta分析文献的报告质量。方法检索维普中文科技期刊数据库、中国知网数据库、中国生物医学文献数据库网络版、万方数据库及Pubmed数据库、Web of science数据库,检索时限从各数据...目的应用Prisma声明评价活血化瘀法治疗中风的系统评价/Meta分析文献的报告质量。方法检索维普中文科技期刊数据库、中国知网数据库、中国生物医学文献数据库网络版、万方数据库及Pubmed数据库、Web of science数据库,检索时限从各数据库建库至2015年7月。查找活血化瘀法治疗中风相关的系统评价/Meta分析文献,由4名评价员按照纳入与排除标准独立筛选文献,并将Prisma声明清单中的27条细化为95个细则,逐条对纳入的文献进行分析。结果纳入16篇系统评价/Meta分析文献,Prisma声明清单中的27条细化的95个细则平均报告率为54.27%。纳入文献问题主要集中体现在方案与注册、文献的检索收集、真实性评价、数据处理及基金支持等方面。结论有关活血化瘀法治疗中风相关的系统评价/Meta分析文献质量不高,尤其在"方法""结果""资金支持"等部分的报告率偏低。展开更多
文摘Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical disability.Thus,in the search for more effective treatments than those already applied for years,which are not entirely efficient,researches have been able to demonstrate the potential of biological strategies using biomaterials to tissue manufacturing through bioengineering and stem cell therapy as a neuroregenerative approach,seeking to promote neuronal recovery after spinal cord injury.Each of these strategies has been developed and meticulously evaluated in several animal models with the aim of analyzing the potential of interventions for neuronal repair and,consequently,boosting functional recovery.Although the majority of experimental research has been conducted in rodents,there is increasing recognition of the importance,and need,of evaluating the safety and efficacy of these interventions in non-human primates before moving to clinical trials involving therapies potentially promising in humans.This article is a literature review from databases(PubMed,Science Direct,Elsevier,Scielo,Redalyc,Cochrane,and NCBI)from 10 years ago to date,using keywords(spinal cord injury,cell therapy,non-human primates,humans,and bioengineering in spinal cord injury).From 110 retrieved articles,after two selection rounds based on inclusion and exclusion criteria,21 articles were analyzed.Thus,this review arises from the need to recognize the experimental therapeutic advances applied in non-human primates and even humans,aimed at deepening these strategies and identifying the advantages and influence of the results on extrapolation for clinical applicability in humans.
文摘As one of the latest research hotspots in the field of artificial intelligence,AI-generated content(AIGC)can create virtual teaching scenarios,such as those involving educational digital humans,through content creation.This integration of AI and education is a product that will profoundly change the form of online education and the learning experience.Currently,most courses for educational digital humans still rely on traditional educational models and face many problems in practice.Based on an overview of AIGC technology and its application in micro-course production,this paper analyzes the current status of micro-courses for educational digital humans.It proposes the application of AIGC technology and educational digital humans in micro-course production,namely,using AIGC technology to drive the transformation of micro-courses for educational digital humans towards intelligence,thereby improving education quality and efficiency.
文摘诊断准确性试验(diagnostic test accuracy,DTA)的系统评价/Meta分析报告规范(PRISMA-DTA)用于规范和提高DTA的系统评价/Meta分析的报告质量,其于2018年1月发表在JAMA杂志。本文对其条目进行解读,以促进国内学者对PRISMA-DTA的理解和应用,以期提高DTA的系统评价/Meta分析报告质量。
基金co-funded by the European Space Agency Dragon5 project[ESA Contract number 4000135216/21/I-NB]co-funded by the Italian Space Agency THERA project[grant number DC-UOT-2019-061].
文摘This research investigates the suitability of PRISMA and Sentinel-2 satellite imagery for retrieving topsoil properties such as Organic Matter(OM),Nitrogen(N),Phosphorus(P),Potassium(K),and pH in croplands using different Machine Learning(ML)algorithms and signal pre-treatments.Ninety-five soil samples were collected in Quzhou County,Northeast China.Satellite images captured soil reflectance data when bare soil was visible.For PRISMA data,a Linear Mixture Model(LMM)was used to separate soil and Photosynthetic Vegetation(PV)endmembers,excluding Non-Photosynthetic Vegetation(NPV)using Band Depth values at the 2100 nm absorption peak of cellulose.Sentinel-2 bare soil reflectance spectra were obtained using thresholds based on NDVI and NBR2 indices.Results showed PRISMA data provided slightly better accuracy in retrieving topsoil nutrients than Sentinel-2.While no optimal predictive algorithm was best,absorbance data proved more effective than reflectance.PRISMA results demonstrated potential for predicting soil nutrients in real scenarios.
基金supported by the National Key Research and Developmental Program of China(2021YFC2700202,2022YFC2702601,2019YFA0802600,2022YFA0806303)National Natural Science Foundation of China(32470915,32000587,32270901,82171601)+1 种基金Global Select Project(DJK-LX-2022010)of the Institute of Health and Medicine,Hefei Comprehensive National Science Center,Joint Fund for New Medicine of USTC(YD9100002034)Scientific Research Foundation for Scholars of the First Affiliated Hospital of USTC(RC2023054)。
文摘Infertility represents a significant health concern,with sperm quantity and quality being crucial determinants of male fertility.Oligoasthenoteratozoospermia(OAT)is characterized by reduced sperm motility,lower sperm concentration,and morphological abnormalities in sperm heads and flagella.Although variants in several genes have been implicated in OAT,its genetic etiologies and pathogenetic mechanisms remain inadequately understood.In this study,we identified a homozygous nonsense mutation(c.916C>T,p.Arg306*)in the coiled-coil domain containing 146(CCDC146)gene in an infertile male patient with OAT.This mutation resulted in the production of a truncated CCDC146 protein(amino acids 1-305),retaining only two out of five coiled-coil domains.To validate the pathogenicity of the CCDC146 mutation,we generated a mouse model(Ccdc146^(mut/mut))with a similar mutation to that of the patient.Consistently,the Ccdc146mut/mut mice exhibited infertility,characterized by significantly reduced sperm counts,diminished motility,and multiple defects in sperm heads and flagella.Furthermore,the levels of axonemal proteins,including DNAH17,DNAH1,and SPAG6,were significantly reduced in the sperm of Ccdc146^(mut/mut) mice.Additionally,both human and mouse CCDC146 interacted with intraflagellar transport protein 20(IFT20),but this interaction was lost in the mutated versions,leading to the degradation of IFT20.This study identified a novel deleterious homozygous nonsense mutation in CCDC146 that causes male infertility,potentially by disrupting axonemal protein transportation.These findings offer valuable insights for genetic counseling and understanding the mechanisms underlying CCDC146 mutant-associated infertility in human males.
文摘网状Meta分析(network Meta-analysis,NMA)目前作为循证医学发展的二代方法学,越来越受到各国卫生技术评估组织的认可。然而,目前NMA的报告存在严重的问题。本文针对NMA的优先报告规范——PRISMA(preferred reporting items for systematic reviews and meta analysis)扩展申明发表在Annals of Internal Medicine上,现将PRISMA扩展申明予以介绍,为国内研究人员撰写和报告高质量的NMA提供借鉴和参考。
文摘目的应用Prisma声明评价活血化瘀法治疗中风的系统评价/Meta分析文献的报告质量。方法检索维普中文科技期刊数据库、中国知网数据库、中国生物医学文献数据库网络版、万方数据库及Pubmed数据库、Web of science数据库,检索时限从各数据库建库至2015年7月。查找活血化瘀法治疗中风相关的系统评价/Meta分析文献,由4名评价员按照纳入与排除标准独立筛选文献,并将Prisma声明清单中的27条细化为95个细则,逐条对纳入的文献进行分析。结果纳入16篇系统评价/Meta分析文献,Prisma声明清单中的27条细化的95个细则平均报告率为54.27%。纳入文献问题主要集中体现在方案与注册、文献的检索收集、真实性评价、数据处理及基金支持等方面。结论有关活血化瘀法治疗中风相关的系统评价/Meta分析文献质量不高,尤其在"方法""结果""资金支持"等部分的报告率偏低。
