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Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II 被引量:10
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作者 郭佳庆 骆艳娥 +3 位作者 范代娣 高鹏飞 马晓轩 朱晨辉 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第5期830-836,共7页
Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production ... Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol·L-1 glucose and 0.019 mol·L-1 nitrogen (carbon-nitrogen ratio, 4.47:1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol·L-1 and 0.017 mol·L-1 , respectively (carbon-nitrogen ratio, 3.82:1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose increased. 展开更多
关键词 carbon-nitrogen ratio human-like collagen organic acid metabolism recombinant Escherichia coli response surface methodology
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Process Control for Production of Human-like Collagen in Fed-batch Culture of Escherichia coli BL 21 被引量:7
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作者 骆艳娥 范代娣 +4 位作者 马晓轩 王德伟 米钰 花秀夫 李稳宏 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2005年第2期276-279,共4页
Recombinant E. coli BL 21 was cultivated in high cell density to produce human-like collagen. The effects of the feeding of nitrogen source, controlled by an auto on/off-feeding mode with two different cycles of 0.5mi... Recombinant E. coli BL 21 was cultivated in high cell density to produce human-like collagen. The effects of the feeding of nitrogen source, controlled by an auto on/off-feeding mode with two different cycles of 0.5min and 4min intervals, oxygen-enrichment methods and inducement strength on the cell yield and human-like collagen production were investigated. The studies showed that nitrogen source feeding in fast cycle could result in higher human-like collagen production than that in slow cycle; and the feedback regulation of glucose, increase of the pressure of fermentation bioreactor, and supply of oxygen-enriched air could all increase cell yield and human-like collagen production. The effects of inducement strength on protein expression were found important. When OD600 reached 90-100, the cultivation temperature was increased to 42℃ to begin induction for 2-3 h, and then shifted to 39℃ for 5-6h induction, the cell density and human-like collagen production could reach 96g·L-1 [DCW (dry cell mass)] and 19.8% (g·g-1 DCW) respectively. 展开更多
关键词 human-like collagen recombinant Escherichia COLI fed-batch culture nitrogen oscillation oxygenenrichment temperature inducement
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Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology 被引量:9
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作者 张驰 范代娣 +4 位作者 尚龙安 马晓轩 骆艳娥 薛文娇 高鹏飞 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第1期137-142,共6页
In order to improve the production of human-like collagen III(HLC III)by fed-batch culture of recombinant Escherichia coli BL21,the Plackett-Burman and Box-Behnken design were applied to optimize the fermentation proc... In order to improve the production of human-like collagen III(HLC III)by fed-batch culture of recombinant Escherichia coli BL21,the Plackett-Burman and Box-Behnken design were applied to optimize the fermentation process parameters.Three variables(induction time,inoculum age and pH),which have significant effects on HLC III production,were selected from eight variables by Plackett-Burman design.With the regression coefficient analysis in the Box-Behnken design,a relationship between HLC III production and three significant factors was obtained,and the optimum levels of the three variables were as follows:induction time 3.2h,inoculum age 12.6 h and pH 6.7.The 3D response surface plots and 2D contour plots created by the Box-Behnken design showed that the interaction between induction time and pH and that between innoculum age and pH were significant.An average 9.68 g·L1HLC III production was attained in the validation experiment under optimized condition,which was 80%higher than the yield of 5.36 g·L1before optimization. 展开更多
关键词 fermentation process parameters human-like collagen III OPTIMIZATION response surface methodology
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Human-Like Decision-Making of Autonomous Vehicles in Dynamic Traffic Scenarios 被引量:4
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作者 Tangyike Zhang Junxiang Zhan +2 位作者 Jiamin Shi Jingmin Xin Nanning Zheng 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2023年第10期1905-1917,共13页
With the maturation of autonomous driving technology, the use of autonomous vehicles in a socially acceptable manner has become a growing demand of the public. Human-like autonomous driving is expected due to the impa... With the maturation of autonomous driving technology, the use of autonomous vehicles in a socially acceptable manner has become a growing demand of the public. Human-like autonomous driving is expected due to the impact of the differences between autonomous vehicles and human drivers on safety.Although human-like decision-making has become a research hotspot, a unified theory has not yet been formed, and there are significant differences in the implementation and performance of existing methods. This paper provides a comprehensive overview of human-like decision-making for autonomous vehicles. The following issues are discussed: 1) The intelligence level of most autonomous driving decision-making algorithms;2) The driving datasets and simulation platforms for testing and verifying human-like decision-making;3) The evaluation metrics of human-likeness;personalized driving;the application of decisionmaking in real traffic scenarios;and 4) The potential research direction of human-like driving. These research results are significant for creating interpretable human-like driving models and applying them in dynamic traffic scenarios. In the future, the combination of intuitive logical reasoning and hierarchical structure will be an important topic for further research. It is expected to meet the needs of human-like driving. 展开更多
关键词 Autonomous vehicles DECISION-MAKING driving behavior human-like driving
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Kinetics of High Cell Density Fed-batch Culture of Recombinant Escherichia coli Producing Human-like Collagen 被引量:3
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作者 花秀夫 范代娣 +6 位作者 骆艳娥 张兮 施惠娟 米钰 马晓轩 尚龙安 赵桂仿 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2006年第2期242-247,共6页
The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded wi... The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and Yx/s were determined as 1.75h, 0.65h^-1 and 0.51g·g^-1, respectively. In the fed-batch culture, different specific growth rates were set at (0.15, 0.2, 0.25h^-1) by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase were obtained. The result shows that the concentrations of cell and product can reach 77.5g·L^-1 and 10.2g·L^-1 respectively. The modal predictions are in good agreement with the experimental data. 展开更多
关键词 human-like collagen KINETICS high density fermentation Escherichia coli
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Progress of Domestic Recombinant Human-Like Collagen Research and Application
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作者 Yu Jianjun Zhao Hua 《China Detergent & Cosmetics》 2022年第2期58-62,共5页
By intercepting the most effective fragment of human collagen’s gene,recombinant human-like collagen is produced by genetic engineering technology and biotechnology.The collagen has good biocompatibility and can prom... By intercepting the most effective fragment of human collagen’s gene,recombinant human-like collagen is produced by genetic engineering technology and biotechnology.The collagen has good biocompatibility and can promote cell formation.Recombinant human-like collagen has great potential application value in medical biological materials,cosmetics and foods.In recent years,many specialized enterprises which are engaged in human-like collagen raw materials production have emerged.In this paper,a brief summary of the current recombinant human-like collagen raw material’s production and its latest research and development have been made. 展开更多
关键词 recombinant human-like collagen FIBRONECTIN genetic recombination biological fermentation
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Towards human-like automated vehicles:review and perspectives on behavioural decision making and intelligent motion planning 被引量:1
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作者 Jiming Xie Yaqin Qin +4 位作者 Yan Zhang Tianshun Chen Bijun Wang Qiyue Zhang Yulan Xia 《Transportation Safety and Environment》 2025年第1期1-21,共21页
Rapid advancements in automated vehicles(AVs)technology have transformed the measurability,controllability and unpredictability of transportation systems.Evaluating driving risk and effectively managing driving behavi... Rapid advancements in automated vehicles(AVs)technology have transformed the measurability,controllability and unpredictability of transportation systems.Evaluating driving risk and effectively managing driving behaviours is critical.AVs should be enabled to identify,analyse,evaluate and devise effective countermeasures for driving risk by autonomously learning from human driving experiences.This learning will enhance the interactive decision-making capabilities and achieve driving behaviours that reflect human-like logic.The primary challenge lies in integrating human-like logic and driving risk constraints based on behavioural decision-making outcomes.This integration is crucial to align the AVs’cognitive levels-movement,comprehension,memory and inference-more closely with human driving necessities,habits and styles.Such alignment holds the potential to improve the prediction and planning of future actions and facilitate the development of motion planning schemes geared towards minimizing driving risk.We performed a comprehensive review of AVs’behavioural decision-making and intelligent motion-planning research from 2000 to 2023 from four key perspectives-driving risk,human-like logic,behavioural decision making and motion planning.Based on the Web of Science and China National Knowledge Internet database,the results of our review indicate that significant progress has been made in AV behavioural decision making and intelligent motion planning over time.When AVs and human-driven vehicles coexist,greater incorporation of human-like logic is required.Guided by these findings,we delineate future development directions and propose a research paradigm for human-like logic and a research framework for human-like logic-driven behavioural decision making and intelligent motion planning of AVs. 展开更多
关键词 driving risk intelligent decision making human-like logic REVIEW intelligent motion planning
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Human-like dexterous manipulation for anthropomorphic five-fingered hands:A review
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作者 Yayu Huang Dongxuan Fan +5 位作者 Haonan Duan Dashun Yan Wen Qi Jia Sun Qian Liu Peng Wang 《Biomimetic Intelligence & Robotics》 2025年第1期35-57,共23页
Humans excel at dexterous manipulation;however,achieving human-level dexterity remains a significant challenge for robots.Technological breakthroughs in the design of anthropomorphic robotic hands,as well as advanceme... Humans excel at dexterous manipulation;however,achieving human-level dexterity remains a significant challenge for robots.Technological breakthroughs in the design of anthropomorphic robotic hands,as well as advancements in visual and tactile perception,have demonstrated significant advantages in addressing this issue.However,coping with the inevitable uncertainty caused by unstructured and dynamic environments in human-like dexterous manipulation tasks,especially for anthropomorphic five-fingered hands,remains an open problem.In this paper,we present a focused review of human-like dexterous manipulation for anthropomorphic five-fingered hands.We begin by defining human-like dexterity and outlining the tasks associated with human-like robot dexterous manipulation.Subsequently,we delve into anthropomorphism and anthropomorphic five-fingered hands,covering definitions,robotic design,and evaluation criteria.Furthermore,we review the learning methods for achieving human-like dexterity in anthropomorphic five-fingered hands,including imitation learning,reinforcement learning and their integration.Finally,we discuss the existing challenges and propose future research directions.This review aims to stimulate interest in scientific research and future applications. 展开更多
关键词 Anthropomorphic five-fingered hands human-like dexterous manipulation Robot learning REVIEW
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Synergistic control of soft robotic hands for human-like grasp postures 被引量:3
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作者 ZHANG NingBin ZHAO Yi +1 位作者 GU GuoYing ZHU XiangYang 《Science China(Technological Sciences)》 SCIE EI CAS CSCD 2022年第3期553-568,共16页
Although significant advances in the design of soft robotic hands have been made to mimic the structure of the human hands,there are great challenges to control them for coordinated and human-like postures.Based on th... Although significant advances in the design of soft robotic hands have been made to mimic the structure of the human hands,there are great challenges to control them for coordinated and human-like postures.Based on the principle of postural synergies in the human hand,we present a synergistic approach for coordinated control of a soft robotic hand to replicate the human-like grasp postures.To this end,we firstly develop a kinematic model to describe the control variables and the various postures of the soft robotic hand.Based on the postural synergies,we use the developed model and Principal Component Analysis(PCA)method to describe the various postures of the soft robotic hand in a low-dimensional space formed by the synergies of actuator motions.Therefore,the coordinates of these synergies can be used as low-dimensional control inputs for the soft robotic hand with a higher-dimensional postural space.Finally,we establish an experimental platform on a customized soft robotic hand with6 pneumatical actuators to verify the effectiveness of the development.Experimental results demonstrate that with only a 2-dimensional control input,the soft robotic hand can reliably replicate 30 grasp postures in the Feix taxonomy of the human hand. 展开更多
关键词 soft robotic hand synergistic control human-like grasp postures principal component analysis pneumatic actuation
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Effect of Temperature on Self-Interaction of Human-Like Collagen 被引量:2
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作者 Fan, Daidi Xing, Jianyu +3 位作者 Xue, Wenjiao Zhu, Chenhui Ma, Xiaoxuan Ma, Rong 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2011年第9期1811-1816,共6页
Effects of temperature on self-interaction of human-like collagen (HLC) were investigated by hydrophobic in- teraction chromatography, calorimetric measurement, and sodium dodecyl sulphate-polyacrylamide gel electro... Effects of temperature on self-interaction of human-like collagen (HLC) were investigated by hydrophobic in- teraction chromatography, calorimetric measurement, and sodium dodecyl sulphate-polyacrylamide gel electropho- resis (SDS-PAGE) analysis. Results show that three types of interaction roles may exist between ttLC molecules at 3--50 ℃, which were divided into three narrower temperature ranges. In temperature range from 3--22 ℃, hy- drogen bonding plays a key role in the formation of a gelatinous aggregate. In the range of 22--38 ℃, hydrophobic bonds accompanied by hydrogen bonds are involved in the formation compact aggregates. When temperature is above 38 ℃ the hydrophobic effect formed in the HLC monomer results in the loss of its ability to self-interact. 展开更多
关键词 CALORIMETRY human-like collagen hydrophobic effect SELF-INTERACTION temperature range
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Human-like lane-changing trajectory planning algorithm for human-machine conflict mitigation 被引量:4
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作者 Changhua Dai Changfu Zong +4 位作者 Dong Zhang Gang Li Kaku Chuyo Hongyu Zheng Fei Gao 《Journal of Intelligent and Connected Vehicles》 EI 2023年第1期46-63,共18页
The purpose of this paper is to alleviate the potential safety problems associated with the human driver and the automatic system competing for the right of way due to different objectives by mitigating the human-mach... The purpose of this paper is to alleviate the potential safety problems associated with the human driver and the automatic system competing for the right of way due to different objectives by mitigating the human-machine conflict phenomenon in human-machine shared driving(HMSD)technology from the automation system.Firstly,a basic lane-changing trajectory algorithm based on the quintic polynomial in the Frenet coordinate system is developed.Then,in order to make the planned trajectory close to human behavior,naturalistic driving data is collected,based on which some lane-changing performance features are selected and analyzed.There are three aspects have been taken into consideration for the human-like lane-changing trajectory:vehicle dynamic stability performance,driving cost optimization,and collision avoidance.Finally,the HMSD experiments are conducted with the driving simulator to test the potential of the human-like lane-changing trajectory planning algorithm.The results demonstrate that the lane-changing trajectory planning algorithm with the highest degree of personalization is highly consistent with human driver behavior and consequently would potentially mitigate the human-machine conflict with the HMSD application.Furthermore,it could be further employed as an empirical trajectory prediction result.The algorithm employs the distribution state of the historical trajectory for human-like processing,simplifying the operational process and ensuring the credibility,integrity,and interpretability of the results.Moreover,in terms of optimization processing,the form of optimization search followed by collision avoidance detection is adopted to in principle reduce the calculation difficulty.Additionally,a new convex polygon collision detection method,namely the vertex embedding method,is proposed for collision avoidance detection. 展开更多
关键词 human-machine conflict human-machine shared driving(HMSD) human-like lane-changing trajectory planning collision avoidance trajectory prediction
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Bioinspired Dual-layered Soft-rigid Gripper for Reduced Damage and Improved Grasping Stability with Real-time Classification
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作者 Wenhui Li Liangsong Huang Yuxia Li 《Journal of Bionic Engineering》 2026年第1期192-224,共33页
Soft grippers research is gaining increasing attention for their flexibility.However,the conventional soft gripper primar-ily focuses on soft fingers,without considering the palm.This makes grasping forces concentrate... Soft grippers research is gaining increasing attention for their flexibility.However,the conventional soft gripper primar-ily focuses on soft fingers,without considering the palm.This makes grasping forces concentrated in the fingertip areas,resulting in objects being prone to damage and instability during handling,especially for delicate items.Additionally,pre-transportation classification faces challenges:tactile methods are complex,visual methods are environment-sensitive,and both struggle with similar objects.To address these problems,inspired by the human hand's transition between finger grasp and palm support and the lotus's hierarchical structure,this paper proposes a dual-layer gripper,named IOSGrip-per.It features four pneumatic soft fingers and a rotational soft-rigid palm.Through coordinated control of the fingers and palm,it transitions concentrated fingertip squeeze force to distributed palm support force,reducing squeeze force and squeeze duration.Moreover,it integrates a range sensor and four load cells,enabling stable and accurate measurements of the object's height and weight.Furthermore,a classifier is developed based on K-nearest neighbor algorithm,allowing real-time object classification.Experiments demonstrate that compared to only using soft fingers,the IOSGripper signifi-cantly reduces the squeeze force on the objects(with 0 N squeeze force during palm support)and damage on the delicate object,while improving grasping stability.Its height and weight measurement errors are within 2 mm and 1 g,respectively.And it achieves high accuracy in three test scenarios,including classifying similar objects.This study provides useful insights for the design of soft grippers capable of human-like grasping and sorting tasks. 展开更多
关键词 Soft-rigid gripper Lotus hierarchical structure human-like grasping Height and weight sensing Realtime classification
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重组胶原蛋白的不同表达体系研究进展
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作者 王啸尘 黄伟 +5 位作者 刘英杰 徐荣荣 王艺纯 张硕 高琛 杨素珍 《山东化工》 2026年第1期99-104,共6页
胶原蛋白是一类具有特征性纤维状结构的细胞外基质蛋白,作为人体结缔组织的主要结构成分,广泛分布于皮肤、骨骼、关节、血管等关键生理部位。胶原蛋白获得途径有传统提取和重组生物技术表达两种,传统提取法可以通过从天然来源,如植物和... 胶原蛋白是一类具有特征性纤维状结构的细胞外基质蛋白,作为人体结缔组织的主要结构成分,广泛分布于皮肤、骨骼、关节、血管等关键生理部位。胶原蛋白获得途径有传统提取和重组生物技术表达两种,传统提取法可以通过从天然来源,如植物和动物中提取获得,而重组生物技术表达则是通过重组蛋白表达系统获得。本文对不同重组胶原蛋白表达体系的开发、研究状况进行系统说明,重点阐述了不同表达体系及其特点,包括原核生物、酵母、杆状病毒、植物及动物细胞,最后对重组胶原蛋白存在的问题进行了综述。 展开更多
关键词 重组胶原蛋白 重组胶原蛋白表达体系 重组类人胶原蛋白
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LncRNA KCNQ1OT1调节miR-218-5p/KLF8轴对肺癌细胞增殖、凋亡和上皮间质转化的影响
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作者 易宣洪 黄晓智 +2 位作者 李志梁 熊伟 王静 《临床肺科杂志》 2026年第2期250-256,共7页
目的探讨长链非编码RNA(LncRNA)KCNQ1OT1调节微小RNA(miR)-218-5p/Krüppel样因子8(KLF8)轴对人肺癌细胞增殖、凋亡和上皮间质转化的影响。方法将A549细胞分为对照(Control)、si-NC、si-KC1、si-KC1+miR-In-NC、si-KC1+miR-218-5p-I... 目的探讨长链非编码RNA(LncRNA)KCNQ1OT1调节微小RNA(miR)-218-5p/Krüppel样因子8(KLF8)轴对人肺癌细胞增殖、凋亡和上皮间质转化的影响。方法将A549细胞分为对照(Control)、si-NC、si-KC1、si-KC1+miR-In-NC、si-KC1+miR-218-5p-In组。qRT-PCR实验检测细胞中LncRNA KCNQ1OT1、miR-218-5p和KLF8 mRNA水平;CCK-8法检测细胞增殖;Annexin V/PI染色法检测细胞凋亡;划痕愈合和Transwell实验检测细胞迁移和侵袭;Western Blot检测细胞中KLF8、E-cadherin、N-cadherin和Bax水平;双荧光素酶实验测定miR-218-5p与LncRNA KCNQ1OT1间的靶向关系。结果与si-NC组相比,si-KC1组细胞存活率、划痕愈合率、细胞侵袭数、LncRNA KCNQ1OT1、KLF8 mRNA以及KLF8、N-cadherin蛋白水平下降,凋亡率、miR-218-5p、Bax和E-cadherin表达上升(P<0.05);与si-KC1+miR-In-NC组相比,si-KC1+miR-218-5p-In组细胞存活率、划痕愈合率、细胞侵袭数、KLF8 mRNA以及KLF8、N-cadherin蛋白水平显著升高(P<0.05),凋亡率、miR-218-5p、E-cadherin和Bax水平显著降低(P<0.05),LncRNA KCNQ1OT1差异无统计学意义(P>0.05);与miR-NC组相比,共转染KC1-WT和miR-218-5p mimic降低细胞中荧光酶活性(P<0.05)。结论敲低LncRNA KCNQ1OT1可能经由miR-218-5p/KLF8轴,诱导人肺癌细胞凋亡,并抑制上皮间质转化和增殖。 展开更多
关键词 长链非编码RNA KCNQ1OT1 微小RNA-218-5p/Krüppel样因子8轴 人肺癌细胞
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不同骨龄生长激素缺乏症患儿应用重组人生长激素治疗的效果分析
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作者 严韶东 杨利 +1 位作者 陈健青 王素云 《中国现代药物应用》 2026年第1期77-81,共5页
目的 评价不同骨龄生长激素缺乏症(GHD)患儿接受重组人生长激素(rhGH)治疗的疗效差异及安全性。方法 回顾性纳入100例GHD患儿,根据骨龄分为青春期前组(骨龄<9岁, 50例)和青春期组(骨龄≥9岁, 50例)。两组均予rhGH治疗。比较两组临床... 目的 评价不同骨龄生长激素缺乏症(GHD)患儿接受重组人生长激素(rhGH)治疗的疗效差异及安全性。方法 回顾性纳入100例GHD患儿,根据骨龄分为青春期前组(骨龄<9岁, 50例)和青春期组(骨龄≥9岁, 50例)。两组均予rhGH治疗。比较两组临床疗效,治疗前后生长速率,治疗后胰岛素样生长因子-1(IGF-1)、25-羟维生素D[25(OH)D]、空腹血糖水平,不良反应发生率。结果 青春期前组治疗总有效率(90.00%)显著高于青春期组(70.00%),差异有统计学意义(P<0.05)。治疗后,两组生长速率均高于治疗前,且青春期前组生长速率(8.47±0.68)cm/年高于青春期组的(6.83±0.81)cm/年,差异有统计学意义(P<0.05)。治疗6、12个月后,青春期前组IGF-1和25(OH)D分别为(145.60±15.23)、(180.30±18.52)ng/ml和(31.13±4.53)、(31.57±4.63)ng/ml,均高于青春期组的(132.47±14.83)、(165.72±17.91)ng/ml和(28.83±4.31)、(29.02±4.23)ng/ml,差异有统计学意义(P<0.05);两组治疗6、12个月后空腹血糖水平比较差异无统计学意义(P>0.05)。青春期前组不良反应发生率为18.00%,青春期组为28.00%,比较差异无统计学意义(P>0.05)。结论 rhGH可显著改善GHD患儿的生长速率与IGF-1水平,其中骨龄较小者受益更大;短期治疗对血糖影响不明显,整体安全性良好,建议早期干预并常规监测甲状腺功能与代谢指标。 展开更多
关键词 生长激素缺乏症 骨龄 重组人生长激素 胰岛素样生长因子-1 25-羟维生素D
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特发性矮小症患儿血清IGF-1、25(OH)D水平与重组人生长激素治疗效果的关系
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作者 魏书培 郭晶晶 +2 位作者 王丹 王秋丽 林芳冰 《分子诊断与治疗杂志》 2026年第2期432-434,共3页
目的探究特发性矮小症(ISS)患儿血清胰岛素样生长因子-1(IGF-1)、维生素D[25(OH)D]水平与重组人生长激素治疗效果的关系。方法回顾性选取2021年1月至2024年1月郑州大学附属儿童医院/河南省儿童医院/郑州儿童医院收治的103例ISS患儿设为... 目的探究特发性矮小症(ISS)患儿血清胰岛素样生长因子-1(IGF-1)、维生素D[25(OH)D]水平与重组人生长激素治疗效果的关系。方法回顾性选取2021年1月至2024年1月郑州大学附属儿童医院/河南省儿童医院/郑州儿童医院收治的103例ISS患儿设为观察组,均予以重组人生长激素治疗。以同时期于本院进行体检的60名健康儿童为对照组。比较治疗前、治疗6、12个月后两组血清IGF-1、25(OH)D、体格指标[身体质量指数(BMI)]、骨龄指标[骨龄年龄差(BAD)、骨龄指数(BAI)]的差异,使用Pearson相关性分析观察组血清IGF-1、25(OH)D水平与体格指标(BMI)、骨龄指标(BAD、BAI)的相关性。结果观察组血清IGF-1、25(OH)D、BMI、BAD、BAI治疗前<治疗6个月后<治疗12个月后,治疗前、治疗6个月后观察组上述指标均低于对照组,差异有统计学意义(P<0.05);Pearson相关性分析结果显示,血清IGF-1、25(OH)D与BMI、BAD、BAI均存在正相关性(P<0.05)。结论ISS儿童血清IGF-1、25(OH)D水平与重组人生长激素治疗效果呈正相关。 展开更多
关键词 特发性矮小症 胰岛素样生长因子-1 维生素D 重组人生长激素
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重组人生长激素联合赖氨酸磷酸氢钙颗粒治疗特发性矮小症疗效观察
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作者 刘娟 刘娜 尹丽 《新乡医学院学报》 2026年第1期37-40,45,共5页
目的探讨重组人生长激素(rhGH)联合赖氨酸磷酸氢钙颗粒治疗特发性矮小症(ISS)的疗效及对患儿骨代谢与生长相关指标的影响。方法选择2020年3月至2024年3月南阳市第二人民医院收治的100例ISS患儿为研究对象。采用随机数字表法将患儿分为... 目的探讨重组人生长激素(rhGH)联合赖氨酸磷酸氢钙颗粒治疗特发性矮小症(ISS)的疗效及对患儿骨代谢与生长相关指标的影响。方法选择2020年3月至2024年3月南阳市第二人民医院收治的100例ISS患儿为研究对象。采用随机数字表法将患儿分为对照组和观察组,每组50例。对照组患儿给予rhGH 0.10~0.15 IU·kg^(-1),每日1次,睡前皮下注射;观察组患儿在此基础上加用赖氨酸磷酸氢钙颗粒,每次5 g,每日2次。2组患儿均连续治疗6个月。治疗前及治疗后3、6个月时测2组患儿的身高、生长速率(GV)及骨龄(BA)。治疗前及治疗后6个月,采集2组患儿空腹静脉血并分离血清,采用酶联免疫吸附法检测胰岛素样生长因子-1(IGF-1)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)水平,电化学发光法检测25-羟基维生素D[25-(OH)D]水平,精氨酸激发试验检测碱性磷酸酶(ALP)水平。记录2组患儿治疗过程中不良反应发生情况,并计算不良反应发生率。结果治疗前2组患儿的身高、GV、BA比较差异无统计学意义(P>0.05)。治疗后3、6个月时,2组患儿的身高、GV、BA显著高于治疗前,且观察组患儿显著高于对照组(P<0.05)。治疗前2组患儿的血清IGF-1、25-(OH)D、ALP水平比较差异无统计学意义(P>0.05)。治疗后6个月,2组患儿的血清IGF-1、25-(OH)D、ALP水平显著高于治疗前,且观察组患儿显著高于对照组(P<0.05)。治疗后6个月时观察组患儿的FT3水平显著高于治疗前,其余各指标2组患者治疗后与治疗前比较差异均无统计学意义(P>0.05)。治疗前及治疗后,2组患儿的FT3、FT4、TSH水平比较差异均无统计学意义(P>0.05)。对照组和观察组患儿不良反应发生率分别为8%(4/50)、6%(3/50),2组患儿不良反应发生率比较差异无统计学意义(χ^(2)=1.628,P=0.804)。结论rhGH联合赖氨酸磷酸氢钙颗粒治疗ISS疗效显著,能够有效推进生长发育进程,提升ALP、IGF-1及25-(OH)D水平,并且未观察到甲状腺功能减退或亚临床甲状腺功能减退的症状和严重的不良反应。 展开更多
关键词 特发性矮小症 重组人生长激素 赖氨酸磷酸氢钙颗粒 胰岛素样生长因子-1 25羟基维生素D 甲状腺功能
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聚乙二醇重组人生长激素治疗特发性矮小症患儿的临床效果
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作者 彭念 袁媛 张荫英 《中国医学创新》 2026年第1期104-108,共5页
目的:探究聚乙二醇重组人生长激素对特发性矮小症(ISS)患儿的影响。方法:选取荆州市第三人民医院儿科2019年1月—2024年6月收治的ISS患儿120例,采用随机数字表法分为对照组(n=60,人生长激素注射液治疗)和观察组(n=60,聚乙二醇重组人生... 目的:探究聚乙二醇重组人生长激素对特发性矮小症(ISS)患儿的影响。方法:选取荆州市第三人民医院儿科2019年1月—2024年6月收治的ISS患儿120例,采用随机数字表法分为对照组(n=60,人生长激素注射液治疗)和观察组(n=60,聚乙二醇重组人生长激素治疗),两组均连续治疗6个月。对比两组胰岛素样生长因子-1(IGF-1)水平、身高与体重、甲状腺功能、骨代谢指标[Ⅰ型胶原交联羧基末端肽(ⅠCTP)、Ⅰ型前胶原氨基端前肽(P1NP)、骨钙素(OC)]及不良反应。结果:治疗后,观察组身高为(135.91±10.51)cm、体重为(35.68±2.12)kg、IGF-1为(333.94±14.09)ng/mL,高于对照组(P<0.05);治疗后,两组血清FT3、FT4及促甲状腺激素(TSH)水平比较,差异无统计学意义(P>0.05);治疗后,两组ⅠCTP、P1NP、OC较治疗前升高,且观察组比对照组高(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:聚乙二醇重组人生长激素能够有效调节ISS患儿的IGF-1水平,对其骨代谢指标具有改善作用,且不影响患儿的甲状腺功能,且不增加不良反应。 展开更多
关键词 聚乙二醇重组人生长激素 特发性矮小症 胰岛素样生长因子-1 甲状腺功能
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