Goals: The aim of this study is to investigate whether there were any association between the 102 T/C and-1438 G/A polymorphisms of the 5-HT2A receptor gene and IBS, and abdominal pain, anxiety and depression. Backgro...Goals: The aim of this study is to investigate whether there were any association between the 102 T/C and-1438 G/A polymorphisms of the 5-HT2A receptor gene and IBS, and abdominal pain, anxiety and depression. Background: Genes involved in serotonin (5-HT)metabolism are good candidates for the pathogenesis of irritable bowel syndrome (IBS). Recently, a silent polymorphism in the 5-HT2A receptor gene was identified that is defined by a T to C transition at position 102. Also, a novel G to A base change at position-1438 of the promoter region has been detected in 5-HT2A receptor gene. Study: Fifty-four patients with IBS diagnosed according to the Rome 1 criteria and 107 healthy individuals were included in the study. PCR was used to amplify a 468-bp (G→ A) and 342-bp (T→ C) fragment of genomic DNA containing the polymorphism. Hospital anxiety and depression scale was used to assess the risk of depression and anxiety. Severity of chronic abdominal pain was determined by visual analogue scale (VAS). Results: It was shown that there was a high incidence of homozygote C allele of the 102T/C polymorphism (22.2% ; OR: 7.89, P =0.04) and homozygote A allele of the-1438 G/A promoter region (37% ; OR: 11.14, P = 0.01) in patients with IBS.The risk of having an anxiety disorder was 83.3% in patients with C/C genotype, which was higher than other allele carrying patients, and overall mean (52.7% ). (x2 = 8.56, P = 0.014). The patients with T/T genotype had a VAS score of 54.93 ± 2.59 mm, which was significantly higher than that of the patients with other genotypes (p1= 0.02, p2 = 0.001). Conclusion: This study suggests that the patients with homozygote C allele of the 102 T/C polymorphism of the 5-HT2A receptor gene, have a high risk of IBS. On the other hand, T/T genotype of 102T/C polymorphism may be associated with more severe pain in patient with IBS.展开更多
Fine mapping of Helminthosporium turcicum resistance gene Ht2 is extremely valuable for map-based cloning of the Ht2 gene, gaining a better knowledge of the distribution of resistance genes in maize genome and marker-...Fine mapping of Helminthosporium turcicum resistance gene Ht2 is extremely valuable for map-based cloning of the Ht2 gene, gaining a better knowledge of the distribution of resistance genes in maize genome and marker-assisted selection in maize breeding. An F2 mapping population was developed from a cross between a resistant inbred line 77Ht2 and a susceptible inbred line Huobai. With the aid of RFLP marker analyses, the Ht2 gene was mapped between the RFLP markers UMC89 and BNL2.369 on chromosome 8, with a genetic distance of 0.9 cM to BNL2.369. There was a linkage between SSR markers UMC1202, BNLG1152, UMC1149 and the Ht2 gene by SSR assay. Among the SSR markers, the genetic distance between UMC1149 and the Ht2 gene was 7.2 cM. By bulked segregant analysis 7 RAPD-amplified products which were probably linked to the Ht2 gene were selected after screening 450 RAPD primers and converted the single-copy ones into SCAR markers. Linkage analysis showed that the genetic distance between the SCAR marker SD-06633 and the Ht2 gene was 0.4 cM. From these results, a part of linkage map around the Ht2 gene was constructed.展开更多
为了探讨5-HT2受体激动剂盐酸2,5-二甲氧基-4-碘苯基丙烷(DOI)对杏仁核突触可塑性的调节作用,本研究在杏仁核脑片上记录基底外侧杏仁核(BLA)场电位,应用单串的θ频率波刺激(TBS)诱导突触可塑性,观察DOI对TBS诱导的突触可塑性的影响,及5-...为了探讨5-HT2受体激动剂盐酸2,5-二甲氧基-4-碘苯基丙烷(DOI)对杏仁核突触可塑性的调节作用,本研究在杏仁核脑片上记录基底外侧杏仁核(BLA)场电位,应用单串的θ频率波刺激(TBS)诱导突触可塑性,观察DOI对TBS诱导的突触可塑性的影响,及5-HT2受体拮抗剂、磷脂酶C抑制剂能否抑制DOI的作用。结果显示:单串的TBS刺激外囊,在BLA仅诱导约为10min的短时程增强。灌流液中加入100μmol/L DOI 20min,对基础的场电位没有作用。但在DOI存在的情况下,单串的TBS即可诱导长时程增强,强直刺激30min后,增强的场电位斜率仍维持在基础值的(162.5±9.7)%(n=9,P<0.01)。DOI对TBS诱导的突触可塑性的易化作用可被5-HT2A/2C受体拮抗剂ketanserin和PLC抑制剂U73122所抑制。以上结果提示5-HT2A/2C受体的激活可通过磷脂酶C通路易化杏仁核的突触可塑性。展开更多
文摘Goals: The aim of this study is to investigate whether there were any association between the 102 T/C and-1438 G/A polymorphisms of the 5-HT2A receptor gene and IBS, and abdominal pain, anxiety and depression. Background: Genes involved in serotonin (5-HT)metabolism are good candidates for the pathogenesis of irritable bowel syndrome (IBS). Recently, a silent polymorphism in the 5-HT2A receptor gene was identified that is defined by a T to C transition at position 102. Also, a novel G to A base change at position-1438 of the promoter region has been detected in 5-HT2A receptor gene. Study: Fifty-four patients with IBS diagnosed according to the Rome 1 criteria and 107 healthy individuals were included in the study. PCR was used to amplify a 468-bp (G→ A) and 342-bp (T→ C) fragment of genomic DNA containing the polymorphism. Hospital anxiety and depression scale was used to assess the risk of depression and anxiety. Severity of chronic abdominal pain was determined by visual analogue scale (VAS). Results: It was shown that there was a high incidence of homozygote C allele of the 102T/C polymorphism (22.2% ; OR: 7.89, P =0.04) and homozygote A allele of the-1438 G/A promoter region (37% ; OR: 11.14, P = 0.01) in patients with IBS.The risk of having an anxiety disorder was 83.3% in patients with C/C genotype, which was higher than other allele carrying patients, and overall mean (52.7% ). (x2 = 8.56, P = 0.014). The patients with T/T genotype had a VAS score of 54.93 ± 2.59 mm, which was significantly higher than that of the patients with other genotypes (p1= 0.02, p2 = 0.001). Conclusion: This study suggests that the patients with homozygote C allele of the 102 T/C polymorphism of the 5-HT2A receptor gene, have a high risk of IBS. On the other hand, T/T genotype of 102T/C polymorphism may be associated with more severe pain in patient with IBS.
基金This work was jointly supported by the National Transgenic Plant Research Foundation of China (Grant No. J00-A-002) and the National Research Foundation for Key Techniques (The Explo-ration and Application of Crop Resources,2001-2003,04-012) of China.
文摘Fine mapping of Helminthosporium turcicum resistance gene Ht2 is extremely valuable for map-based cloning of the Ht2 gene, gaining a better knowledge of the distribution of resistance genes in maize genome and marker-assisted selection in maize breeding. An F2 mapping population was developed from a cross between a resistant inbred line 77Ht2 and a susceptible inbred line Huobai. With the aid of RFLP marker analyses, the Ht2 gene was mapped between the RFLP markers UMC89 and BNL2.369 on chromosome 8, with a genetic distance of 0.9 cM to BNL2.369. There was a linkage between SSR markers UMC1202, BNLG1152, UMC1149 and the Ht2 gene by SSR assay. Among the SSR markers, the genetic distance between UMC1149 and the Ht2 gene was 7.2 cM. By bulked segregant analysis 7 RAPD-amplified products which were probably linked to the Ht2 gene were selected after screening 450 RAPD primers and converted the single-copy ones into SCAR markers. Linkage analysis showed that the genetic distance between the SCAR marker SD-06633 and the Ht2 gene was 0.4 cM. From these results, a part of linkage map around the Ht2 gene was constructed.
文摘为了探讨5-HT2受体激动剂盐酸2,5-二甲氧基-4-碘苯基丙烷(DOI)对杏仁核突触可塑性的调节作用,本研究在杏仁核脑片上记录基底外侧杏仁核(BLA)场电位,应用单串的θ频率波刺激(TBS)诱导突触可塑性,观察DOI对TBS诱导的突触可塑性的影响,及5-HT2受体拮抗剂、磷脂酶C抑制剂能否抑制DOI的作用。结果显示:单串的TBS刺激外囊,在BLA仅诱导约为10min的短时程增强。灌流液中加入100μmol/L DOI 20min,对基础的场电位没有作用。但在DOI存在的情况下,单串的TBS即可诱导长时程增强,强直刺激30min后,增强的场电位斜率仍维持在基础值的(162.5±9.7)%(n=9,P<0.01)。DOI对TBS诱导的突触可塑性的易化作用可被5-HT2A/2C受体拮抗剂ketanserin和PLC抑制剂U73122所抑制。以上结果提示5-HT2A/2C受体的激活可通过磷脂酶C通路易化杏仁核的突触可塑性。
