目的探讨原发性肝细胞癌(HCC)组织中热休克蛋白70-2(HSPA2)的表达及临床意义。方法利用实时荧光定量PCR(RT-q PCR)和免疫组化法检测120例HCC组织和癌旁组织中的HSPA2 m RNA和蛋白的表达水平,并分析HSPA2蛋白在HCC组织中的表达水平与患...目的探讨原发性肝细胞癌(HCC)组织中热休克蛋白70-2(HSPA2)的表达及临床意义。方法利用实时荧光定量PCR(RT-q PCR)和免疫组化法检测120例HCC组织和癌旁组织中的HSPA2 m RNA和蛋白的表达水平,并分析HSPA2蛋白在HCC组织中的表达水平与患者临床病理特征之间的关系。结果 RT-q PCR结果显示,HSPA2 m RNA在50例HCC组织中的表达水平明显高于癌旁正常肝脏组织中(2.82±0.19与1.18±0.14,P<0.01);免疫组化结果显示,在120例HCC标本中,HSPA2蛋白高表达有69例(57.5%),低表达有51例(42.5%),HSPA2蛋白高表达与肿瘤长径、病理分化程度和肿瘤分期有关(P<0.05),而与年龄、性别、HBs Ag状态以及血清AFP水平无关(P>0.05)。结论 HSPA2在人HCC中高表达,其高表达可能与HCC的发生、发展密切相关,可能作为HCC早期诊断和预后预测的指标。展开更多
Objective Our aim was to explore whether heat stress protein(HSP) 9 preferentially expresses under heat stress and affects the expression of other heat stress proteins as well as to explore the effect of HSPB9 overexp...Objective Our aim was to explore whether heat stress protein(HSP) 9 preferentially expresses under heat stress and affects the expression of other heat stress proteins as well as to explore the effect of HSPB9 overexpression and knockdown on apoptosis in DF-1. Methods We used gene cloning to construct an overexpression vector of the target gene, and synthesized the target gene interference fragment to transfect the chicken fibroblast cell line. Gene and protein expression, as well as apoptosis, were detected by RT-qPCR, Western blot, and flow cytometry. Results Chicken DF-1 cells showed an early state of apoptosis in the early stages of HSPB9 overexpression. In the later stages, as HSPB9 expression increased, the cells showed inhibition of apoptosis. When the cells were under heat stress, HSPB9 expression was much higher and earlier than the expression of HSPB1 and HSPA2. In addition, high expression of HSPB9 had a negative effect on HSPB1 and HSPA2 expression. This negative feedback decreased the percentage of early stages of apoptotic cells and promoted cell survival. Conclusion HSPB9 expression, although rapid, is detrimental to cell survival early during its overexpression. In heat stress, HSPB9 overexpression, while inhibiting the expression of HSPA2 and HSPB1, is beneficial to cell survival.展开更多
文摘目的探讨原发性肝细胞癌(HCC)组织中热休克蛋白70-2(HSPA2)的表达及临床意义。方法利用实时荧光定量PCR(RT-q PCR)和免疫组化法检测120例HCC组织和癌旁组织中的HSPA2 m RNA和蛋白的表达水平,并分析HSPA2蛋白在HCC组织中的表达水平与患者临床病理特征之间的关系。结果 RT-q PCR结果显示,HSPA2 m RNA在50例HCC组织中的表达水平明显高于癌旁正常肝脏组织中(2.82±0.19与1.18±0.14,P<0.01);免疫组化结果显示,在120例HCC标本中,HSPA2蛋白高表达有69例(57.5%),低表达有51例(42.5%),HSPA2蛋白高表达与肿瘤长径、病理分化程度和肿瘤分期有关(P<0.05),而与年龄、性别、HBs Ag状态以及血清AFP水平无关(P>0.05)。结论 HSPA2在人HCC中高表达,其高表达可能与HCC的发生、发展密切相关,可能作为HCC早期诊断和预后预测的指标。
基金supported by grants from the National Science and Technology Support Program of China [2014BAD08B08]National Natural Science Foundation of China [30972093]
文摘Objective Our aim was to explore whether heat stress protein(HSP) 9 preferentially expresses under heat stress and affects the expression of other heat stress proteins as well as to explore the effect of HSPB9 overexpression and knockdown on apoptosis in DF-1. Methods We used gene cloning to construct an overexpression vector of the target gene, and synthesized the target gene interference fragment to transfect the chicken fibroblast cell line. Gene and protein expression, as well as apoptosis, were detected by RT-qPCR, Western blot, and flow cytometry. Results Chicken DF-1 cells showed an early state of apoptosis in the early stages of HSPB9 overexpression. In the later stages, as HSPB9 expression increased, the cells showed inhibition of apoptosis. When the cells were under heat stress, HSPB9 expression was much higher and earlier than the expression of HSPB1 and HSPA2. In addition, high expression of HSPB9 had a negative effect on HSPB1 and HSPA2 expression. This negative feedback decreased the percentage of early stages of apoptotic cells and promoted cell survival. Conclusion HSPB9 expression, although rapid, is detrimental to cell survival early during its overexpression. In heat stress, HSPB9 overexpression, while inhibiting the expression of HSPA2 and HSPB1, is beneficial to cell survival.
