Previous investigations suggest association between obesity and Ala16Val MnSOD gene polymorphism. The V allele produces enzyme which not catalyze the superoxide anion efficiently as occurs with A allele. As obesity is...Previous investigations suggest association between obesity and Ala16Val MnSOD gene polymorphism. The V allele produces enzyme which not catalyze the superoxide anion efficiently as occurs with A allele. As obesity is related to development of other metabolic disorders we performed a study that analyzed the effect of interaction between Ala16Val MnSOD polymorphism and obesity on lipid, oxidative and inflammatory biomarkers of adult subjects. The study enrolled 161 volunteers as categorized in six groups with different genotypes: Obeses with different genotypes (AAO, VVO and AVO) and nonobese (AANO, VVNO and AVNO). In general the group AANO presented lower values whereas VVO presented higher values of biomarkers analyzed. These results suggest that oxidative metabolism influenced by genetic status could to minimize or maximize the obesity effects on lipid, oxidative and inflammatory biomarkers that are also implicated in the genesis of important dysfunctions and diseases as atherosclerosis, diabetes 2 and cardiovascular morbidities.展开更多
Using ligation-mediated PCR method to study the status of DNA-protein interaction at hypersensitive site 2 of locus control Region and βmaj promoter of MEL cell line before and after induction, MEL cell has been cult...Using ligation-mediated PCR method to study the status of DNA-protein interaction at hypersensitive site 2 of locus control Region and βmaj promoter of MEL cell line before and after induction, MEL cell has been cultured and induced to differentiation by Hemin and DMSO, then the live cells have been treated with dimethyl sulfate. Ligation mediated PCR has been carried out following the chemical cleavage. The results demonstrate that before and after induction, the status of DNA-protein interaction at both hypersensitive site 2 and βmaj promoter change significantly, indicating that distal regulatory elements (locus control region, hypersensitive sites) as well as proximal regulatory elements (promoter, enhancer) of β-globin gene cluster participate in the regulation of developmental specificity.展开更多
文摘Previous investigations suggest association between obesity and Ala16Val MnSOD gene polymorphism. The V allele produces enzyme which not catalyze the superoxide anion efficiently as occurs with A allele. As obesity is related to development of other metabolic disorders we performed a study that analyzed the effect of interaction between Ala16Val MnSOD polymorphism and obesity on lipid, oxidative and inflammatory biomarkers of adult subjects. The study enrolled 161 volunteers as categorized in six groups with different genotypes: Obeses with different genotypes (AAO, VVO and AVO) and nonobese (AANO, VVNO and AVNO). In general the group AANO presented lower values whereas VVO presented higher values of biomarkers analyzed. These results suggest that oxidative metabolism influenced by genetic status could to minimize or maximize the obesity effects on lipid, oxidative and inflammatory biomarkers that are also implicated in the genesis of important dysfunctions and diseases as atherosclerosis, diabetes 2 and cardiovascular morbidities.
文摘Using ligation-mediated PCR method to study the status of DNA-protein interaction at hypersensitive site 2 of locus control Region and βmaj promoter of MEL cell line before and after induction, MEL cell has been cultured and induced to differentiation by Hemin and DMSO, then the live cells have been treated with dimethyl sulfate. Ligation mediated PCR has been carried out following the chemical cleavage. The results demonstrate that before and after induction, the status of DNA-protein interaction at both hypersensitive site 2 and βmaj promoter change significantly, indicating that distal regulatory elements (locus control region, hypersensitive sites) as well as proximal regulatory elements (promoter, enhancer) of β-globin gene cluster participate in the regulation of developmental specificity.
