Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water ex...Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied. Results EST electrophoresis showed that EST was an important antidotal enzyme system and reacted strongly to environment EST changed greatly during the whole exposure period so that it could be viewed as a pathological index of toxicity. Extracts decreased the glycogen content of the snails＇ soft tissues greatly, and also the protein content. ConcIusion All extracts show strong molluscicidal activity. The LD50 value of the water extract of N. indicum Mill is as low as 13.2 mg/L. EST can be viewed as a pathological index of toxicity. The energy metabolism abnormity is the key reason fnr the molluscicidal activities. The biochemical mechanism needs further research.展开更多
Objective:To investigate the chemical constituents of Polygala japonica Houtt.. Methods:The constituents were separated and purified by column chromatographies with silical gel, RP-silical gel and Sephadex LH-20. Thei...Objective:To investigate the chemical constituents of Polygala japonica Houtt.. Methods:The constituents were separated and purified by column chromatographies with silical gel, RP-silical gel and Sephadex LH-20. Their structures were elucidated on the basis of spectral data (IR, MS, 1H-NMR, 13C-NMR, HMQC and HMBC). Results: Totally 15 compounds were obtained from Polygala japonica Houtt. And their structures were identified as castaprenol-12 (Ⅰ), β-sitosterol (Ⅱ), stigmasterol (Ⅲ),stigmasta-7,22-di-ene-3-one (Ⅳ), n-dotriacontanol ( Ⅴ ), n-hexadecanol ( Ⅵ ), arachidic acid (Ⅶ), erogosterol (Ⅷ), kaempferol (Ⅸ), quercetin (Ⅹ), daucosterol (Ⅺ), p-hydroxybenzoic acid (Ⅻ),coumaric acid (ⅩⅢ), rhamnocitrin 3-O-β-D-galactopyranoside (ⅪⅤ), quercetin 3-O-β-D-glucopyranoside (ⅩⅤ). Conclusion: Compounds Ⅰ , Ⅲ , Ⅳ, Ⅴ , Ⅵ, Ⅶ, Ⅷ, Ⅻ , ⅩⅢ and ⅩⅣ were obtained from this plant for the first time.展开更多
[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japon...[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japonica Houtt. were analyzed by the headspace solid-phase microextraction( HS-SPME) combined with GC-MS,and the relative percentage of each component was determined by peak area normalization. [Results] Thirty kinds of volatile components were identified from the leaves and rhizomes of P. japonica Houtt.,mainly including olefins,aromatic hydrocarbons,alkanes and esters. [Conclusions] The volatile components in different parts of P. japonica Houtt. were different,and there may be difference in the medicinal value of volatile components in different parts,thus it is required to take an overall consideration of these differences in the development and utilization of P. japonica Houtt.展开更多
Non-alcoholic fatty liver disease(NAFLD)is intricately linked to dysregulated hepatic lipid metabolism and gut microbiota imbalances.This study explores the potential mechanisms by which AEN,a lignan-rich extract from...Non-alcoholic fatty liver disease(NAFLD)is intricately linked to dysregulated hepatic lipid metabolism and gut microbiota imbalances.This study explores the potential mechanisms by which AEN,a lignan-rich extract from Myristica fragrans Houtt.,alleviates NAFLD by affecting gut bacteria,bile acid metabolism,and fecal chemical composition.We demonstrate that AEN prevents weight gain,lipid accumulation,and enhances liver function in mice subjected to a high-fat diet.Utilizing a multi-omics strategy that includes microbiome analysis and metabolomics,we observed significant shifts in gut microbial composition and fecal metabolites,notably an increase in lithocholic acid,taurocholic acid,and the ratio of non-12-OH to 12-OH conjugated bile acids.These metabolic changes were accompanied by a decrease in bile acid metabolism-related enzymes,such as bile salt hydrolase,in the AEN group compared to the high-fat diet group.Moreover,AEN downregulated the farnesoid X receptor-fibroblast growth factor 15 pathway in the intestine,promoting the alternative bile acid synthesis pathway.The elucidation of the correlation between changes in the microbiome and fecal metabolites suggests that AEN modulates specific gut microbes,thereby influencing bile acid metabolism.This modulation was evident in the increased abundance of beneficial bacteria related to bile acid production,such as Parabacteroides and Bilophila,and the decreased abundance of harmful bacteria like Helicobacter and Streptococcus.Network pharmacology and GC×GC-TOF-MSsuggested that licarin A and B could be key bioactive lignans in AEN.These findings underscore the potential of AEN in countering NAFLD by targeting the gut-liver axis,offering a novel therapeutic avenue for NAFLD management.展开更多
The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 ch...The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 chemical components were identified by gas chromatography-mass spectrometry (GC-MS)method.The cellulase extraction (CE)method was made a camparison with distillation extraction (DSE)method.展开更多
基金This work was supported by the National Natural Science Foundation of China (No. 30471506).
文摘Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied. Results EST electrophoresis showed that EST was an important antidotal enzyme system and reacted strongly to environment EST changed greatly during the whole exposure period so that it could be viewed as a pathological index of toxicity. Extracts decreased the glycogen content of the snails＇ soft tissues greatly, and also the protein content. ConcIusion All extracts show strong molluscicidal activity. The LD50 value of the water extract of N. indicum Mill is as low as 13.2 mg/L. EST can be viewed as a pathological index of toxicity. The energy metabolism abnormity is the key reason fnr the molluscicidal activities. The biochemical mechanism needs further research.
基金Supported by the 863 Foundation of China ( No.2003AA2Z3507 ) the Scientific Foundation of Shanghai,China (No. 03QMH1414, 04DZ19843, 04DZ19856 and 04DZ19857)
文摘Objective:To investigate the chemical constituents of Polygala japonica Houtt.. Methods:The constituents were separated and purified by column chromatographies with silical gel, RP-silical gel and Sephadex LH-20. Their structures were elucidated on the basis of spectral data (IR, MS, 1H-NMR, 13C-NMR, HMQC and HMBC). Results: Totally 15 compounds were obtained from Polygala japonica Houtt. And their structures were identified as castaprenol-12 (Ⅰ), β-sitosterol (Ⅱ), stigmasterol (Ⅲ),stigmasta-7,22-di-ene-3-one (Ⅳ), n-dotriacontanol ( Ⅴ ), n-hexadecanol ( Ⅵ ), arachidic acid (Ⅶ), erogosterol (Ⅷ), kaempferol (Ⅸ), quercetin (Ⅹ), daucosterol (Ⅺ), p-hydroxybenzoic acid (Ⅻ),coumaric acid (ⅩⅢ), rhamnocitrin 3-O-β-D-galactopyranoside (ⅪⅤ), quercetin 3-O-β-D-glucopyranoside (ⅩⅤ). Conclusion: Compounds Ⅰ , Ⅲ , Ⅳ, Ⅴ , Ⅵ, Ⅶ, Ⅷ, Ⅻ , ⅩⅢ and ⅩⅣ were obtained from this plant for the first time.
文摘[Objectives] To analyze the volatile components in different parts of Polygala japonica Houtt. and compare the differences of these volatile components. [Methods] The volatile components in different parts of P. japonica Houtt. were analyzed by the headspace solid-phase microextraction( HS-SPME) combined with GC-MS,and the relative percentage of each component was determined by peak area normalization. [Results] Thirty kinds of volatile components were identified from the leaves and rhizomes of P. japonica Houtt.,mainly including olefins,aromatic hydrocarbons,alkanes and esters. [Conclusions] The volatile components in different parts of P. japonica Houtt. were different,and there may be difference in the medicinal value of volatile components in different parts,thus it is required to take an overall consideration of these differences in the development and utilization of P. japonica Houtt.
基金supported by National Nature Science Foundation of China(32272332,31972052,31820103010,32021005)the Fundamental Reach Funds for Central Universities(JUSRP622020,JUSRP22006,JUSRP51501)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.
文摘Non-alcoholic fatty liver disease(NAFLD)is intricately linked to dysregulated hepatic lipid metabolism and gut microbiota imbalances.This study explores the potential mechanisms by which AEN,a lignan-rich extract from Myristica fragrans Houtt.,alleviates NAFLD by affecting gut bacteria,bile acid metabolism,and fecal chemical composition.We demonstrate that AEN prevents weight gain,lipid accumulation,and enhances liver function in mice subjected to a high-fat diet.Utilizing a multi-omics strategy that includes microbiome analysis and metabolomics,we observed significant shifts in gut microbial composition and fecal metabolites,notably an increase in lithocholic acid,taurocholic acid,and the ratio of non-12-OH to 12-OH conjugated bile acids.These metabolic changes were accompanied by a decrease in bile acid metabolism-related enzymes,such as bile salt hydrolase,in the AEN group compared to the high-fat diet group.Moreover,AEN downregulated the farnesoid X receptor-fibroblast growth factor 15 pathway in the intestine,promoting the alternative bile acid synthesis pathway.The elucidation of the correlation between changes in the microbiome and fecal metabolites suggests that AEN modulates specific gut microbes,thereby influencing bile acid metabolism.This modulation was evident in the increased abundance of beneficial bacteria related to bile acid production,such as Parabacteroides and Bilophila,and the decreased abundance of harmful bacteria like Helicobacter and Streptococcus.Network pharmacology and GC×GC-TOF-MSsuggested that licarin A and B could be key bioactive lignans in AEN.These findings underscore the potential of AEN in countering NAFLD by targeting the gut-liver axis,offering a novel therapeutic avenue for NAFLD management.
文摘The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 chemical components were identified by gas chromatography-mass spectrometry (GC-MS)method.The cellulase extraction (CE)method was made a camparison with distillation extraction (DSE)method.
