A total of 13 types of endophytic fungi,classified into six genera,were isolated from Hosta.ventricosa based on 18 S rDNA sequencing of ITS region and microscopic examination.The antioxidant activities of the crude ex...A total of 13 types of endophytic fungi,classified into six genera,were isolated from Hosta.ventricosa based on 18 S rDNA sequencing of ITS region and microscopic examination.The antioxidant activities of the crude extracts were investigated by T-AOC,DPPH and ABTS＋ methods.The results showed that the crude extracts from Fusarium oxysporum（HoV1） and Epicoccum（HoV4）,which presented higher phenol levels（1.90±0.03 mg/g,and 2.41±0.01 mg/g,respectively）,exhibited stronger comprehensive antioxidant activities,with higher T-AOC values（63.46±0.26 U/g,and 66.05±0.71 U/g,respectively）,lower EC_（50） values against ABTS~＋（30.25±0.05 μg/m L,and 24.66±0.65 μg/m L,respectively） and DPPH radicals（83.42±0.24 μg/m L,and 59.136 μg/m L,respectively）.Moreover,Fusarium oxysporum（Ho V8） showed excellent scavenging activity against DPPH radicals（EC_（50） = 16.02 μg/m L）,which was better than BHT.All of them could be useful in the developing of new natural antioxidant agents.展开更多
基金National Natural Science Foundation of China(Grant No.81703387)the Foundation for the demonstration of tea-fruit-medicine organic ecological safety belt in Ya’an
文摘A total of 13 types of endophytic fungi,classified into six genera,were isolated from Hosta.ventricosa based on 18 S rDNA sequencing of ITS region and microscopic examination.The antioxidant activities of the crude extracts were investigated by T-AOC,DPPH and ABTS＋ methods.The results showed that the crude extracts from Fusarium oxysporum（HoV1） and Epicoccum（HoV4）,which presented higher phenol levels（1.90±0.03 mg/g,and 2.41±0.01 mg/g,respectively）,exhibited stronger comprehensive antioxidant activities,with higher T-AOC values（63.46±0.26 U/g,and 66.05±0.71 U/g,respectively）,lower EC_（50） values against ABTS~＋（30.25±0.05 μg/m L,and 24.66±0.65 μg/m L,respectively） and DPPH radicals（83.42±0.24 μg/m L,and 59.136 μg/m L,respectively）.Moreover,Fusarium oxysporum（Ho V8） showed excellent scavenging activity against DPPH radicals（EC_（50） = 16.02 μg/m L）,which was better than BHT.All of them could be useful in the developing of new natural antioxidant agents.
