The effect of sowing date on grain protein, hordein fraction content and malting quality of two-rowed spring barley was investigated by using ten commercial cultivars with different grain protein content and the relat...The effect of sowing date on grain protein, hordein fraction content and malting quality of two-rowed spring barley was investigated by using ten commercial cultivars with different grain protein content and the relationships among these traits were examined. The results showed that grain protein content and B hordein content increased as the sowing date postponed and were significantly affected by sowing date, while C and D hordein contents were less influenced by sowing date. There were significant differences in grain protein and hordein fraction content among the ten cultivars. The coefficient of variation of D hordein content was much larger than that of B and C hordein contents, suggesting its greater variation caused by different sowing dates. Beta-amylase activity and diastatic power were also significantly affected by sowing date, with malt extract being less affected. Significant differences in measured malt quality were found among the ten cultivars. Grain protein was significantly correlated with B hordein and malt extract positively and negatively, respectively. There was no significant correlation between beta-amylase activity or diastatic power and grain protein content. B hordein was negatively and significantly correlated with malt extract, but no significant correlations between C hordein, D hordein and malting quality traits.展开更多
Proteomic studies in barley have been predominantly focused on malting barley for the brewing industry.In this study,protein contents and detailed protein compositions of grains of Wild Barley(WB)(Hordeum spontaneum)a...Proteomic studies in barley have been predominantly focused on malting barley for the brewing industry.In this study,protein contents and detailed protein compositions of grains of Wild Barley(WB)(Hordeum spontaneum)and two varieties of domesticated barley(Hordeum vulgare),Golden Promise(GP),bred for brewing,and PS3,bred for food,were investigated to reveal compositional differences in the hordeins,the major storage proteins of barley.For proteomic profiling a sensitive LC-MS/MS Orbitrap system was used in combination with a one-step denaturing and reducing phenol extraction.The extracts were subjected to label-free bottom-up proteomics.Differences were revealed not only in the overall composition of groups of storage proteins,but also in individual hordeins.WB and PS3 contained higher amounts of D-hordein than GP,while GP had higher levels of B-hordein.Furthermore,the differences in protein composition were evaluated to identify links between the proteome and genotypes’use case and the transition from wild barley.展开更多
基金Project supported by the National Natural Science Foundation of China (Nos. 30270779 and 30471022) and Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) (No. 20020335028), China
文摘The effect of sowing date on grain protein, hordein fraction content and malting quality of two-rowed spring barley was investigated by using ten commercial cultivars with different grain protein content and the relationships among these traits were examined. The results showed that grain protein content and B hordein content increased as the sowing date postponed and were significantly affected by sowing date, while C and D hordein contents were less influenced by sowing date. There were significant differences in grain protein and hordein fraction content among the ten cultivars. The coefficient of variation of D hordein content was much larger than that of B and C hordein contents, suggesting its greater variation caused by different sowing dates. Beta-amylase activity and diastatic power were also significantly affected by sowing date, with malt extract being less affected. Significant differences in measured malt quality were found among the ten cultivars. Grain protein was significantly correlated with B hordein and malt extract positively and negatively, respectively. There was no significant correlation between beta-amylase activity or diastatic power and grain protein content. B hordein was negatively and significantly correlated with malt extract, but no significant correlations between C hordein, D hordein and malting quality traits.
基金funded by Independent Research Fund Denmark(DFF)via the project Barley:From Feed to Food(grant no 10.46540/2035-00057B)by the Austrian Science Fund(FWF)(grant no10.55776/P33891)Graduate School of Technical Sciences(GSTS).
文摘Proteomic studies in barley have been predominantly focused on malting barley for the brewing industry.In this study,protein contents and detailed protein compositions of grains of Wild Barley(WB)(Hordeum spontaneum)and two varieties of domesticated barley(Hordeum vulgare),Golden Promise(GP),bred for brewing,and PS3,bred for food,were investigated to reveal compositional differences in the hordeins,the major storage proteins of barley.For proteomic profiling a sensitive LC-MS/MS Orbitrap system was used in combination with a one-step denaturing and reducing phenol extraction.The extracts were subjected to label-free bottom-up proteomics.Differences were revealed not only in the overall composition of groups of storage proteins,but also in individual hordeins.WB and PS3 contained higher amounts of D-hordein than GP,while GP had higher levels of B-hordein.Furthermore,the differences in protein composition were evaluated to identify links between the proteome and genotypes’use case and the transition from wild barley.
