The amidourea-based homoduplex was developed as a super organogelator,which could form stable gels in wide-tested solvents.And the reversible gel to solution transition was responsive to heat/cool and acid/base stimul...The amidourea-based homoduplex was developed as a super organogelator,which could form stable gels in wide-tested solvents.And the reversible gel to solution transition was responsive to heat/cool and acid/base stimuli.The organogels were extensively investigated by 1 H NMR,UV-visible absorption spectroscopy,fluorescence spectroscopy,scanning electron microscopy,transmission electron microscopy and powder X-ray diffraction.Based on these data,the gelation mechanism was rationally proposed.The hydrogen-bonded homoduplexes served as the basic assembling units,and further aggregated into three dimensional networks via-stacking and van der Waals interactions,which consequently led to the entangled fibers for the gel formation.展开更多
A modified low denaturing temperature PCR (LDT-PCR) method combined with DNA microarray technique is developed in our lab for quick and effective identification of various mutations in an 81 base pair region of Mycoba...A modified low denaturing temperature PCR (LDT-PCR) method combined with DNA microarray technique is developed in our lab for quick and effective identification of various mutations in an 81 base pair region of Mycobacterium Tuberculosis (MTB) ribosome RNA polymerase subunit B (rpoB) gene associated with rifampin resistance. By incurporation of wild type (wt) allele fragments that had been PCR amplified previously, the target PCR fragments coming from mutant clinical MTB samples were codenaturized with incorporated wt type allele fragment at 94°C and then let them randomly form matched structures (homoduplex) and allele mismatch-containing structures (heteroduplex), respectively, when the temperature cooled down to 70°C. After the temperature was raised to 80°C, the heteroduplex double stranded fragments were preferentially denatured and resulted in PCR amplification as well as fluorescence incurporation. Since the homoduplex fragments need a higher temperature to be denatured, they were kept in double-stranded status at that temperature and failed to be PCR amplified. By hybridization of LDT-PCR products with the probes spotted on microarray slides, the fluorescent signals representing the presence of gene mutations were detected. We have tested this method on 35 clinical MTB samples and obtained satisfied results.展开更多
基金supported by the National Natural Science Foundation of China (91127009)the National Basic Research Program of China(2011CB932501)
文摘The amidourea-based homoduplex was developed as a super organogelator,which could form stable gels in wide-tested solvents.And the reversible gel to solution transition was responsive to heat/cool and acid/base stimuli.The organogels were extensively investigated by 1 H NMR,UV-visible absorption spectroscopy,fluorescence spectroscopy,scanning electron microscopy,transmission electron microscopy and powder X-ray diffraction.Based on these data,the gelation mechanism was rationally proposed.The hydrogen-bonded homoduplexes served as the basic assembling units,and further aggregated into three dimensional networks via-stacking and van der Waals interactions,which consequently led to the entangled fibers for the gel formation.
文摘A modified low denaturing temperature PCR (LDT-PCR) method combined with DNA microarray technique is developed in our lab for quick and effective identification of various mutations in an 81 base pair region of Mycobacterium Tuberculosis (MTB) ribosome RNA polymerase subunit B (rpoB) gene associated with rifampin resistance. By incurporation of wild type (wt) allele fragments that had been PCR amplified previously, the target PCR fragments coming from mutant clinical MTB samples were codenaturized with incorporated wt type allele fragment at 94°C and then let them randomly form matched structures (homoduplex) and allele mismatch-containing structures (heteroduplex), respectively, when the temperature cooled down to 70°C. After the temperature was raised to 80°C, the heteroduplex double stranded fragments were preferentially denatured and resulted in PCR amplification as well as fluorescence incurporation. Since the homoduplex fragments need a higher temperature to be denatured, they were kept in double-stranded status at that temperature and failed to be PCR amplified. By hybridization of LDT-PCR products with the probes spotted on microarray slides, the fluorescent signals representing the presence of gene mutations were detected. We have tested this method on 35 clinical MTB samples and obtained satisfied results.
