In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology bas...In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology based on the principle of high-throughput sequencing,and established a multi-locus 10 animalderived components identification method of cattle,goat,sheep,donkey,horse,chicken,duck,goose,pigeon,quail in meat and meat products.The specific loci of each species could be detected and the species could be accurately identified,including 5 loci for cattle and duck,3 loci for sheep,9 loci for chicken and horse,10 loci for goose and pigeon,6 loci for quail and 1 locus for donkey and goat,and an adulteration model was established to simulate commercially available samples.The results showed that the method established in this study had high throughput,good repeatability and accuracy,and was able to identify 10 animalderived components simultaneously with 100%repeatability accuracy.The detection limit was 0.1%(m/m)in simulated samples of chicken,duck and horse.Using the method established in this study to test commercially available samples,4 samples from 14 commercially available samples were detected to be inconsistent with the labels,of which 2 did not contain the target ingredient and 2 were adulterated with small amounts of other ingredients.展开更多
The introduction of next-generation sequencing(NGS) technology in testing for hereditary cancer susceptibility allows testing of multiple cancer susceptibility genes simultaneously. While there are many potential bene...The introduction of next-generation sequencing(NGS) technology in testing for hereditary cancer susceptibility allows testing of multiple cancer susceptibility genes simultaneously. While there are many potential benefits to utilizing this technology in the hereditary cancer clinic, including efficiency of time and cost, there are also important limitations that must be considered. The best panel for the given clinical situation should be selected to minimize the number of variants of unknown significance. The inclusion in panels of low penetrance or newly identified genes without specific actionability can be problematic for interpretation.Genetic counselors are an essential part of the hereditary cancer risk assessment team, helping the medical team select the most appropriate test and interpret the often complex results. Genetic counselors obtain an extended family history, counsel patients on the available tests and the potential implications of results for themselves and their family members(pre-test counseling), explain to patients the implications of the test results(post-test counseling), and assist in testing family members at risk.展开更多
The Liaoning clawed salamander(Onychodactylus zhaoermii)is an endemic and critically endangered amphibian species of China.To study the population genetics of natural populations of this species,32 samples were collec...The Liaoning clawed salamander(Onychodactylus zhaoermii)is an endemic and critically endangered amphibian species of China.To study the population genetics of natural populations of this species,32 samples were collected from six different locations,and the mitochondrial genome was sequenced.Population genetic analyses showed that the Liaoning clawed salamander is composed of only one radialized cluster with ultralow nucleotide diversity.Late Pleistocene climate cooling(-100 to-30 kya)may have reduced the effective population size of the Liaoning clawed salamander,and the subsequent temperature increase(~25 kya to present)provided the opportunity for population expansion.Because of heat sensitivity,the maximum temperature of the prebreeding period,especially from March to May,is very important for the surface environment living in the Liaoning clawed salamander.Three suitable regions were predicted by the MaxEnt model,and the largest suitable region(approximately 899 km2)was at the four-county boundary area and was larger than the present’Natural Conservation Community of the Liaoning Clawed Salamander’.To ensure more effective protection of all Liaoning clawed salamanders,we suggest extending the current’Natural Conservation Community of the Liaoning Clawed Salamander’to include the four-county boundary area.展开更多
In this study, the genetic diversity of 51 cultivars in the primary core collection of peach （Prunus persica （L.） Batsch） was evaluated by using simple sequence repeats （SSRs）. The phylogenetic relationships and...In this study, the genetic diversity of 51 cultivars in the primary core collection of peach （Prunus persica （L.） Batsch） was evaluated by using simple sequence repeats （SSRs）. The phylogenetic relationships and the evolutionary history among different cultivars were determined on the basis of SSR data. Twenty-two polymorphic SSR primer pairs were selected, and a total of 111 alleles were identified in the 51 cultivars, with an average of 5 alleles per locus. According to traditional Chinese classification of peach cultivars, the 51 cultivars in the peach primary core collection belong to six variety groups. The SSR analysis revealed that the levels of the genetic diversity within each variety group were ranked as Sweet peach 〉 Crisp peach 〉 Flat peach 〉 Nectarine 〉 Honey Peach 〉 Yellow fleshed peach. The genetic diversity among the Chinese cultivars was higher than that among the introduced cultivars. Cluster analysis by the unweighted pair group method with arithmetic averaging （UPGMA） placed the 51 cultivars into five linkage clusters. Cultivar members from the same variety group were distributed in different UPGMA clusters and some members from different variety groups were placed under the same cluster. Different variety groups could not be differentiated in accordance with SSR markers. The SSR analysis revealed rich genetic diversity in the peach primary core collection, representative of genetic resources of peach.展开更多
针对在水下无线光通信(Underwater Wireless Optical Communication,UWOC)中使用直流偏置光正交频分复用(Direct Current Optical-Orthogonal Frequency Division Multiplexing,DCOOFDM)技术导致的峰均比(Peak-to-Average Power Ratio,P...针对在水下无线光通信(Underwater Wireless Optical Communication,UWOC)中使用直流偏置光正交频分复用(Direct Current Optical-Orthogonal Frequency Division Multiplexing,DCOOFDM)技术导致的峰均比(Peak-to-Average Power Ratio,PAPR)过高的问题,基于部分传输序列(Partial Transmit Sequence,PTS)方法,使用粒子群优化-遗传因子算法(Partial Swarm Optimization-Genetic Algorithm,PSO-GA)联合算法寻找最佳相位因子,以降低PAPR。仿真结果表明,使用联合算法寻找最佳相位因子,在互补累积分布函数(Complementary Cumulative Distribution Function,CCDF)为10-4时,可以使得系统的峰均比降低约3 dB。相较于传统PTS算法与限幅算法,PSO-GA算法在保证PAPR性能改善的前提下,没有牺牲误码率性能。展开更多
Synthetic biology relies on the screening and quantification of genetic components to assemble sophisticated gene circuits with specific functions.Microscopy is a powerful tool for characterizing complex cellular phen...Synthetic biology relies on the screening and quantification of genetic components to assemble sophisticated gene circuits with specific functions.Microscopy is a powerful tool for characterizing complex cellular phenotypes with increasing spatial and temporal resolution to library screening of genetic elements.Microscopy-based assays are powerful tools for characterizing cellular phenotypes with spatial and temporal resolution and can be applied to large-scale samples for library screening of genetic elements.However,strategies for high-throughput microscopy experiments remain limited.Here,we present a high-throughput,microscopy-based platform that can simultaneously complete the preparation of an 8×12-well agarose pad plate,allowing for the screening of 96 independent strains or experimental conditions in a single experiment.Using this platform,we screened a library of natural intrinsic promoters from Pseudomonas aeruginosa and identified a small subset of robust promoters that drives stable levels of gene expression under varying growth conditions.Additionally,the platform allowed for single-cell measurement of genetic elements over time,enabling the identification of complex and dynamic phenotypes to map genotype in high throughput.We expected that the platform could be employed to accelerate the identification and characterization of genetic elements in various biological systems,as well as to understand the relationship between cellular phenotypes and internal states,including genotypes and gene expression programs.展开更多
基金financially supported by National Key R&D Program(2021YFF0701905)。
文摘In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology based on the principle of high-throughput sequencing,and established a multi-locus 10 animalderived components identification method of cattle,goat,sheep,donkey,horse,chicken,duck,goose,pigeon,quail in meat and meat products.The specific loci of each species could be detected and the species could be accurately identified,including 5 loci for cattle and duck,3 loci for sheep,9 loci for chicken and horse,10 loci for goose and pigeon,6 loci for quail and 1 locus for donkey and goat,and an adulteration model was established to simulate commercially available samples.The results showed that the method established in this study had high throughput,good repeatability and accuracy,and was able to identify 10 animalderived components simultaneously with 100%repeatability accuracy.The detection limit was 0.1%(m/m)in simulated samples of chicken,duck and horse.Using the method established in this study to test commercially available samples,4 samples from 14 commercially available samples were detected to be inconsistent with the labels,of which 2 did not contain the target ingredient and 2 were adulterated with small amounts of other ingredients.
文摘The introduction of next-generation sequencing(NGS) technology in testing for hereditary cancer susceptibility allows testing of multiple cancer susceptibility genes simultaneously. While there are many potential benefits to utilizing this technology in the hereditary cancer clinic, including efficiency of time and cost, there are also important limitations that must be considered. The best panel for the given clinical situation should be selected to minimize the number of variants of unknown significance. The inclusion in panels of low penetrance or newly identified genes without specific actionability can be problematic for interpretation.Genetic counselors are an essential part of the hereditary cancer risk assessment team, helping the medical team select the most appropriate test and interpret the often complex results. Genetic counselors obtain an extended family history, counsel patients on the available tests and the potential implications of results for themselves and their family members(pre-test counseling), explain to patients the implications of the test results(post-test counseling), and assist in testing family members at risk.
基金supported financially by the Research Fund for the Educational Commission of Liaoning Province of China(LQN201904 and LJC201901)the Doctoral Program of Shenyang Normal University(Grant No.BS201821)the Natural Science Foundation of Liaoning Province of China(2020-BS-148 and 2021-MS-241)。
文摘The Liaoning clawed salamander(Onychodactylus zhaoermii)is an endemic and critically endangered amphibian species of China.To study the population genetics of natural populations of this species,32 samples were collected from six different locations,and the mitochondrial genome was sequenced.Population genetic analyses showed that the Liaoning clawed salamander is composed of only one radialized cluster with ultralow nucleotide diversity.Late Pleistocene climate cooling(-100 to-30 kya)may have reduced the effective population size of the Liaoning clawed salamander,and the subsequent temperature increase(~25 kya to present)provided the opportunity for population expansion.Because of heat sensitivity,the maximum temperature of the prebreeding period,especially from March to May,is very important for the surface environment living in the Liaoning clawed salamander.Three suitable regions were predicted by the MaxEnt model,and the largest suitable region(approximately 899 km2)was at the four-county boundary area and was larger than the present’Natural Conservation Community of the Liaoning Clawed Salamander’.To ensure more effective protection of all Liaoning clawed salamanders,we suggest extending the current’Natural Conservation Community of the Liaoning Clawed Salamander’to include the four-county boundary area.
基金Supported by Beijing Municipal Education Commission Urban Agriculture Disciplines Development Project (XK1001900553)Program for new Century Excellent Talents in University of China (NCET-06-0108).
文摘In this study, the genetic diversity of 51 cultivars in the primary core collection of peach （Prunus persica （L.） Batsch） was evaluated by using simple sequence repeats （SSRs）. The phylogenetic relationships and the evolutionary history among different cultivars were determined on the basis of SSR data. Twenty-two polymorphic SSR primer pairs were selected, and a total of 111 alleles were identified in the 51 cultivars, with an average of 5 alleles per locus. According to traditional Chinese classification of peach cultivars, the 51 cultivars in the peach primary core collection belong to six variety groups. The SSR analysis revealed that the levels of the genetic diversity within each variety group were ranked as Sweet peach 〉 Crisp peach 〉 Flat peach 〉 Nectarine 〉 Honey Peach 〉 Yellow fleshed peach. The genetic diversity among the Chinese cultivars was higher than that among the introduced cultivars. Cluster analysis by the unweighted pair group method with arithmetic averaging （UPGMA） placed the 51 cultivars into five linkage clusters. Cultivar members from the same variety group were distributed in different UPGMA clusters and some members from different variety groups were placed under the same cluster. Different variety groups could not be differentiated in accordance with SSR markers. The SSR analysis revealed rich genetic diversity in the peach primary core collection, representative of genetic resources of peach.
文摘针对在水下无线光通信(Underwater Wireless Optical Communication,UWOC)中使用直流偏置光正交频分复用(Direct Current Optical-Orthogonal Frequency Division Multiplexing,DCOOFDM)技术导致的峰均比(Peak-to-Average Power Ratio,PAPR)过高的问题,基于部分传输序列(Partial Transmit Sequence,PTS)方法,使用粒子群优化-遗传因子算法(Partial Swarm Optimization-Genetic Algorithm,PSO-GA)联合算法寻找最佳相位因子,以降低PAPR。仿真结果表明,使用联合算法寻找最佳相位因子,在互补累积分布函数(Complementary Cumulative Distribution Function,CCDF)为10-4时,可以使得系统的峰均比降低约3 dB。相较于传统PTS算法与限幅算法,PSO-GA算法在保证PAPR性能改善的前提下,没有牺牲误码率性能。
基金supported by the National Key Research and Development Program of China (Grant No.2020YFA0906900 to Fan Jin)the National Natural Science Foundation of China (Grant No.32000061 to Rongrong Zhang and 32101177 to Yajia Huang)Shenzhen Engineering Research Center of Therapeutic Synthetic Microbes (Grant No.XMHT20220104015 to Fan Jin)。
文摘Synthetic biology relies on the screening and quantification of genetic components to assemble sophisticated gene circuits with specific functions.Microscopy is a powerful tool for characterizing complex cellular phenotypes with increasing spatial and temporal resolution to library screening of genetic elements.Microscopy-based assays are powerful tools for characterizing cellular phenotypes with spatial and temporal resolution and can be applied to large-scale samples for library screening of genetic elements.However,strategies for high-throughput microscopy experiments remain limited.Here,we present a high-throughput,microscopy-based platform that can simultaneously complete the preparation of an 8×12-well agarose pad plate,allowing for the screening of 96 independent strains or experimental conditions in a single experiment.Using this platform,we screened a library of natural intrinsic promoters from Pseudomonas aeruginosa and identified a small subset of robust promoters that drives stable levels of gene expression under varying growth conditions.Additionally,the platform allowed for single-cell measurement of genetic elements over time,enabling the identification of complex and dynamic phenotypes to map genotype in high throughput.We expected that the platform could be employed to accelerate the identification and characterization of genetic elements in various biological systems,as well as to understand the relationship between cellular phenotypes and internal states,including genotypes and gene expression programs.
