Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer(LET)irradiation and to develop an accurate model of radiation exposure based on the cytoki...Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer(LET)irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation.Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery(MSD),a high-throughput and sensitive electrochemiluminescence measurement technique.Dose estimation equations were set up using multiple linear regression analysis.Results The relative levels of IL-6 at 1 h,IL-5 and IL-6 at 24 h,and IL-5,IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently.The minimum measured levels of IL-5,IL-6 and IL-15 were up to 4.0076 pg/mL,16.4538 pg/mL and 0.4150 pg/mL,respectively.In addition,dose estimation models were established and verified.Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5,IL-6 and IL-15.These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators.Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.展开更多
This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression ...This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression levels of apoptotic factors in mice following exposure to acute irradiation after 7 days of simulated microgravity.The results demonstrated that significant reductions in spermatozoa,primary spermatocytes and spermatogonia,and increased globular cells in seminiferous tubule and pro-apoptotic proteins were observed in the group exposed to over0.4 Gy irradiation.Collectively,the data suggest that lesions inflicted by simulated microgravity are not markedly modified by lower doses of irradiation(0.2 Gy)in mouse testis compared to the control group.However,testicular impairments were markedly evident in the group exposed to higher doses of carbon ions plus simulated microgravity,which may be due at least in part to elevated apoptosis initiated by the mitochondrial apoptosis pathway in germ cells.展开更多
目的：采用Meta分析方法评价let-7低表达在肺癌预后中的作用及高迁移率旋蛋白A2（HMGA2）表达与肺癌关系的临床意义。方法：计算机检索PubMed、Web of Science、Medline、Cochrane Library、Google、Scholar、CNKI、CBM、万方和维普等...目的：采用Meta分析方法评价let-7低表达在肺癌预后中的作用及高迁移率旋蛋白A2（HMGA2）表达与肺癌关系的临床意义。方法：计算机检索PubMed、Web of Science、Medline、Cochrane Library、Google、Scholar、CNKI、CBM、万方和维普等数据库,收集关于let-7低表达与肺癌预后及HMGA2与肺癌发生发展的关联性研究,采用StataSE 12.0软件进行Meta分析。结果：共纳入10篇关于let-7低表达与肺癌预后关系的文献用于Meta分析,均为非小细胞肺癌患者。let-7低表达预示着肺癌患者较差的总生存率,风险比（HR）的合并值为1.55（95%CI：1.16～2.09,P〈0.05）。共纳入9篇关于HMGA2表达与非小细胞肺癌发生发展关系的病例对照研究用于Meta分析,肺癌组织中HMGA2表达阳性率明显高于癌旁正常组织（OR=5.92,95%CI：3.96～8.85,P=0.000）。HMGA2在肺癌组织的表达与肺癌TNM分期、有无淋巴结转移有明显关联（OR=0.26,95%CI：0.16～0.41,P〈0.05;OR=0.29,95%CI：0.12～0.71,P〈0.05）。结论：肺癌患者中let-7低表达是肺癌患者预后的危险因素。HMGA2表达阳性率越高,肺癌发生的概率就越高,越具有侵袭性。展开更多
空间高能质子和重离子是导致元器件发生单粒子效应的根本原因,为准确评估元器件在轨遭遇的单粒子效应风险,必须清楚高能质子、重离子与器件材料发生核反应的物理过程及生成的次级重离子LET(Line Energy Transfer)分布规律。针对典型CMO...空间高能质子和重离子是导致元器件发生单粒子效应的根本原因,为准确评估元器件在轨遭遇的单粒子效应风险,必须清楚高能质子、重离子与器件材料发生核反应的物理过程及生成的次级重离子LET(Line Energy Transfer)分布规律。针对典型CMOS工艺器件模拟计算了不同能量质子和氦核粒子在器件灵敏单元内产生的反冲核、平均能量及线性能量转移值,并分析了半导体器件金属布线层中重金属对次级重离子LET分布的影响规律。计算结果表明:高能粒子与器件相互作用后产生大量次级重离子,且高能质子作用后产生的次级粒子的LET值主要分布为0~25MeV·cm^2/mg;高能氦核粒子作用后产生的次级粒子的LET值主要分布为0~35 MeV·cm^2/mg;有重金属钨(W)存在时能提高次级粒子的LET值,增加了半导体器件发生单粒子效应的概率,该研究结果可为元器件单粒子效应风险分析、航天器抗单粒子效应指标确定提供重要依据。展开更多
目的:观察藏医佐木阿汤及放血疗法对高原红细胞增多症(H A P C)模型大鼠血常规中红细胞(RBC)、血红蛋白(Hb)和红细胞压积(HCT)及血清促红细胞生成素(EPO)的影响。方法:选用雄性Wistar大鼠56只,随机分为平原对照组、高原模型组、药物组...目的:观察藏医佐木阿汤及放血疗法对高原红细胞增多症(H A P C)模型大鼠血常规中红细胞(RBC)、血红蛋白(Hb)和红细胞压积(HCT)及血清促红细胞生成素(EPO)的影响。方法:选用雄性Wistar大鼠56只,随机分为平原对照组、高原模型组、药物组、放血组、药物加放血组。除平原对照组外,余组均饲养于拉萨以建立HAPC模型。饲养3个月后分别进行佐木阿汤灌胃和放血干预,干预3个月后取血进行血常规及EPO检测。结果:高原模型组大鼠RBC、Hb、HCT、EPO含量均较平原对照组升高(P<0.05);与高原模型组比较,药物组、放血组、药物加放血组干预后以上生化指标均降低,且药物组和药物加放血组效果更好(P<0.05),放血组仅对于RBC、EPO含量的影响具有统计学意义(P<0.05)。干预三组间比较均没有统计学意义。结论:藏医佐木阿汤与放血疗法对于HAPC的防治有效果。展开更多
眼晶状体是人体对辐射最敏感的组织之一,电离辐射的生物效应因其传能线密度(linear energy transfer,LET)的不同而不同,同等吸收剂量条件下,高LET辐射生物效应比低LET辐射更强,尽管目前辐射对眼晶状体的影响这一领域已经有了较多研究,...眼晶状体是人体对辐射最敏感的组织之一,电离辐射的生物效应因其传能线密度(linear energy transfer,LET)的不同而不同,同等吸收剂量条件下,高LET辐射生物效应比低LET辐射更强,尽管目前辐射对眼晶状体的影响这一领域已经有了较多研究,但对于高LET对眼晶状体的影响知之甚少。本文简述人眼晶状体剂量限值的历史变化情况,综述各种高LET粒子的生物学实验,调查高LET辐射眼晶状体的流行病学,简要分析高原白内障高患病率的原因,并初步讨论未来研究高LET需要考虑的因素,旨在为国内今后开展高LET辐射对人眼晶状体的研究提供有价值的信息。展开更多
基金supported by the National Natural Science Foundation of China[11635013,11705248,U1832101]National Key Research and Development Program of China[2017YFC0108605]the Science and Technology Research Project of Gansu Province[No.145RTSA012 and 17JR5RA307]。
文摘Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer(LET)irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation.Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery(MSD),a high-throughput and sensitive electrochemiluminescence measurement technique.Dose estimation equations were set up using multiple linear regression analysis.Results The relative levels of IL-6 at 1 h,IL-5 and IL-6 at 24 h,and IL-5,IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently.The minimum measured levels of IL-5,IL-6 and IL-15 were up to 4.0076 pg/mL,16.4538 pg/mL and 0.4150 pg/mL,respectively.In addition,dose estimation models were established and verified.Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5,IL-6 and IL-15.These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators.Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.
基金Supported by grants from the National Basic Research Program of China(No.2010CB834202)Knowledge Innovation Projection of Chinese Academy of Science(No.KJCX2-YW-L08)The National Natural Science Foundation of China(No.10835011,No.10675151,No.41161058,No.31060065 and No.10805064)
文摘This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression levels of apoptotic factors in mice following exposure to acute irradiation after 7 days of simulated microgravity.The results demonstrated that significant reductions in spermatozoa,primary spermatocytes and spermatogonia,and increased globular cells in seminiferous tubule and pro-apoptotic proteins were observed in the group exposed to over0.4 Gy irradiation.Collectively,the data suggest that lesions inflicted by simulated microgravity are not markedly modified by lower doses of irradiation(0.2 Gy)in mouse testis compared to the control group.However,testicular impairments were markedly evident in the group exposed to higher doses of carbon ions plus simulated microgravity,which may be due at least in part to elevated apoptosis initiated by the mitochondrial apoptosis pathway in germ cells.
文摘目的：采用Meta分析方法评价let-7低表达在肺癌预后中的作用及高迁移率旋蛋白A2（HMGA2）表达与肺癌关系的临床意义。方法：计算机检索PubMed、Web of Science、Medline、Cochrane Library、Google、Scholar、CNKI、CBM、万方和维普等数据库,收集关于let-7低表达与肺癌预后及HMGA2与肺癌发生发展的关联性研究,采用StataSE 12.0软件进行Meta分析。结果：共纳入10篇关于let-7低表达与肺癌预后关系的文献用于Meta分析,均为非小细胞肺癌患者。let-7低表达预示着肺癌患者较差的总生存率,风险比（HR）的合并值为1.55（95%CI：1.16～2.09,P〈0.05）。共纳入9篇关于HMGA2表达与非小细胞肺癌发生发展关系的病例对照研究用于Meta分析,肺癌组织中HMGA2表达阳性率明显高于癌旁正常组织（OR=5.92,95%CI：3.96～8.85,P=0.000）。HMGA2在肺癌组织的表达与肺癌TNM分期、有无淋巴结转移有明显关联（OR=0.26,95%CI：0.16～0.41,P〈0.05;OR=0.29,95%CI：0.12～0.71,P〈0.05）。结论：肺癌患者中let-7低表达是肺癌患者预后的危险因素。HMGA2表达阳性率越高,肺癌发生的概率就越高,越具有侵袭性。
文摘空间高能质子和重离子是导致元器件发生单粒子效应的根本原因,为准确评估元器件在轨遭遇的单粒子效应风险,必须清楚高能质子、重离子与器件材料发生核反应的物理过程及生成的次级重离子LET(Line Energy Transfer)分布规律。针对典型CMOS工艺器件模拟计算了不同能量质子和氦核粒子在器件灵敏单元内产生的反冲核、平均能量及线性能量转移值,并分析了半导体器件金属布线层中重金属对次级重离子LET分布的影响规律。计算结果表明:高能粒子与器件相互作用后产生大量次级重离子,且高能质子作用后产生的次级粒子的LET值主要分布为0~25MeV·cm^2/mg;高能氦核粒子作用后产生的次级粒子的LET值主要分布为0~35 MeV·cm^2/mg;有重金属钨(W)存在时能提高次级粒子的LET值,增加了半导体器件发生单粒子效应的概率,该研究结果可为元器件单粒子效应风险分析、航天器抗单粒子效应指标确定提供重要依据。
文摘目的:观察藏医佐木阿汤及放血疗法对高原红细胞增多症(H A P C)模型大鼠血常规中红细胞(RBC)、血红蛋白(Hb)和红细胞压积(HCT)及血清促红细胞生成素(EPO)的影响。方法:选用雄性Wistar大鼠56只,随机分为平原对照组、高原模型组、药物组、放血组、药物加放血组。除平原对照组外,余组均饲养于拉萨以建立HAPC模型。饲养3个月后分别进行佐木阿汤灌胃和放血干预,干预3个月后取血进行血常规及EPO检测。结果:高原模型组大鼠RBC、Hb、HCT、EPO含量均较平原对照组升高(P<0.05);与高原模型组比较,药物组、放血组、药物加放血组干预后以上生化指标均降低,且药物组和药物加放血组效果更好(P<0.05),放血组仅对于RBC、EPO含量的影响具有统计学意义(P<0.05)。干预三组间比较均没有统计学意义。结论:藏医佐木阿汤与放血疗法对于HAPC的防治有效果。
文摘眼晶状体是人体对辐射最敏感的组织之一,电离辐射的生物效应因其传能线密度(linear energy transfer,LET)的不同而不同,同等吸收剂量条件下,高LET辐射生物效应比低LET辐射更强,尽管目前辐射对眼晶状体的影响这一领域已经有了较多研究,但对于高LET对眼晶状体的影响知之甚少。本文简述人眼晶状体剂量限值的历史变化情况,综述各种高LET粒子的生物学实验,调查高LET辐射眼晶状体的流行病学,简要分析高原白内障高患病率的原因,并初步讨论未来研究高LET需要考虑的因素,旨在为国内今后开展高LET辐射对人眼晶状体的研究提供有价值的信息。
