[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular mar...[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources.展开更多
[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The...[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The influence of kenaf variety,hormone for callus induction,and explant type on the regeneration was examined.[Result]The optimal variety and explant for the regeneration were K89 and cotyledon,respectively.The 6-benzylaminopurine(6-BA)was suitable for callus induction and the optimum concentration was 2 mg/L.In addition,with this cytokinin,1.7~4.0 adventitious buds were produced,and 27.7%~38.3% of the adventitious buds could grow to plants.Adventitious roots can be induced with 0.2 mg/L naphthylacetic acid(NAA)and 0.1 mg/L indole-3-acetic acid(IAA).[Conclusion]Cotyledon of K89 had huge potential for the regeneration of kenaf.展开更多
Light-weight composite panels were manufactured using kenaf core particles as core material and kenaf bast fiber-woven sheets as top and bottom surfaces. Methylene diphenyldiisocyanate (MDI) resin was used as the adhe...Light-weight composite panels were manufactured using kenaf core particles as core material and kenaf bast fiber-woven sheets as top and bottom surfaces. Methylene diphenyldiisocyanate (MDI) resin was used as the adhesive with the resin content of 4% for core particles and 50 g/m2 for bast fiber- woven sheets. The target board densities were set at 0.35, 0.45 and 0.55 g/cm3. The composite panels were evaluated with Japanese Industrial Standard for Particleboards (JIS A 5908- 2003).The results show that the composite panel has high modulus of rupture and internal bonding strength. The properties of 0.45 g/cm3 density composite panel are: MOR 20.4 MPa, MOE 1.94 MPa, IB 0.36 MPa, WA142%, TS 21%. Kenaf is a good raw material for making light-weight composite panels.展开更多
基金Supported by The National High Technology Research and Development Program of China(2001AA241211)Industry Special:Studyon the Efficient Production and Harvest Technique in Ramee, Flax,Jute/Kenaf(NYHYJX07-18)~~
文摘[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources.
文摘[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The influence of kenaf variety,hormone for callus induction,and explant type on the regeneration was examined.[Result]The optimal variety and explant for the regeneration were K89 and cotyledon,respectively.The 6-benzylaminopurine(6-BA)was suitable for callus induction and the optimum concentration was 2 mg/L.In addition,with this cytokinin,1.7~4.0 adventitious buds were produced,and 27.7%~38.3% of the adventitious buds could grow to plants.Adventitious roots can be induced with 0.2 mg/L naphthylacetic acid(NAA)and 0.1 mg/L indole-3-acetic acid(IAA).[Conclusion]Cotyledon of K89 had huge potential for the regeneration of kenaf.
基金Supported by the talent fund of the Central South University of Forestry and Technology.
文摘Light-weight composite panels were manufactured using kenaf core particles as core material and kenaf bast fiber-woven sheets as top and bottom surfaces. Methylene diphenyldiisocyanate (MDI) resin was used as the adhesive with the resin content of 4% for core particles and 50 g/m2 for bast fiber- woven sheets. The target board densities were set at 0.35, 0.45 and 0.55 g/cm3. The composite panels were evaluated with Japanese Industrial Standard for Particleboards (JIS A 5908- 2003).The results show that the composite panel has high modulus of rupture and internal bonding strength. The properties of 0.45 g/cm3 density composite panel are: MOR 20.4 MPa, MOE 1.94 MPa, IB 0.36 MPa, WA142%, TS 21%. Kenaf is a good raw material for making light-weight composite panels.
