Lysimachia capillipes(L.capillipes)Hemsl,belong to primulaceae pearl vegetables genus,is a treasure in traditional Chinese medicine.It has the effects of invigorating qi and tonifying deficiency,dispelling wind and ac...Lysimachia capillipes(L.capillipes)Hemsl,belong to primulaceae pearl vegetables genus,is a treasure in traditional Chinese medicine.It has the effects of invigorating qi and tonifying deficiency,dispelling wind and activating blood,awakening the brain,relieving cough and regulating menstruation.With the development of modern medicine,the active components and therapeutic mechanisms of L.capillipes Hemsl have been gradually revealed.The present report systematically reviews the chemical composition and biological activities of L.capillipes Hemsl,to provide scientific basis and reference for detailed research on L.capillipes Hemsl.展开更多
Aim To investigate the chemical constituents of Salvia cavaleriei Levi. var. erythrophylla （Hemsl.） Stib. Methods Compounds were isolated by silica gel column chromatography and Sephadex LH-20 gel column chromatogra...Aim To investigate the chemical constituents of Salvia cavaleriei Levi. var. erythrophylla （Hemsl.） Stib. Methods Compounds were isolated by silica gel column chromatography and Sephadex LH-20 gel column chromatography, and the structures were identified by spectral analysis. Result Five compounds were identified as 3β, 6β, 23-trihydroxyolean-12-en-28-oic acid （1）, ursolic acid （2）, n-heptadecanoic acid （3）, n-octadecanoic acid （4）, and β-sitosterol （5）. Conclusion Five compounds were isolated from the plant for the first time.展开更多
As a widely used traditional Chinese medicine （TCM）, Swertia punicea Hemsl has exhibited effects on anti-hepatitis B virus （HBV）, liver protection, hypoglycemic activity and cholecystitis. In this study, we confir...As a widely used traditional Chinese medicine （TCM）, Swertia punicea Hemsl has exhibited effects on anti-hepatitis B virus （HBV）, liver protection, hypoglycemic activity and cholecystitis. In this study, we confirmed that xanthone extract from Swertia punicea Hemsl （XSPH） improved the motor deficit, increased the levels of striatal dopamine （DA） and homovanilic acid （HVA）, and alleviated the loss of tyrosine hydroxylase （TH）-positive neurons located in substantia nigra pars compacta （SNpc） in MPTP-induced mouse model of Parkinson＇s disease （PD）. In conclusion, the present results indicated that XSPH offered neuroprotective effects against the neurotoxicity of MPTP and it might be a potential treatment for PD.展开更多
A new compound named 1-hydrate, 3-deoxy-α-D-tagatofuranose was isolated from Swertia punicea Hemsl. The structure of compound was determined by 1D and 2D NMR, HRESIMS techniques.
Two eremophilane-type sesquiterpenoids from the rhizomes of Ligularia veitchiana（Hemsl.） Greenm were isolated and determined as 10α,11-endoperoxide-1（2）-en-7α-hydroeremophilane（1） and 1（10）-en-2-oxo-7α-isop...Two eremophilane-type sesquiterpenoids from the rhizomes of Ligularia veitchiana（Hemsl.） Greenm were isolated and determined as 10α,11-endoperoxide-1（2）-en-7α-hydroeremophilane（1） and 1（10）-en-2-oxo-7α-isopropanol-eremophilane（2）.Compound 1 was a new compound and compound 2 was a new natural product.The structures of the two compounds were established by means of spectral methods including 1^H NMR,13^C NMR,2D NMR and MS.展开更多
Objective:To study the effects of the ethnic medicine Polygala fallax Hemsl with Guangxi characteristics on the sex hormones andβ-EP in research objective perimenopausal rat models.Methods:40 female SPF rats were ran...Objective:To study the effects of the ethnic medicine Polygala fallax Hemsl with Guangxi characteristics on the sex hormones andβ-EP in research objective perimenopausal rat models.Methods:40 female SPF rats were randomly divided into 4 groups,including the normal,model,high-dose and lowdose groups.Rats of three groups except for the normal one were treated with perimenopausal modelling through the method of subcutaneous injection of compound 4-VCD for 15 consecutive days.Rats of the normal and model group were normally fed without any treatment.Rats of the high-dose and low-dose groups were administered by high-and low-dose intragastric administration of the extract of Polygala fallax Hemsl.According to the menstrual cycle of the vaginal smear of the rat,each menstrual cycle is a course of treatment and 6 consecutive courses of treatment would be given.The indexes of serum sex hormones(E2,FSH,LH)andβ-EP of rats in each group were observed after treatment.Results:After the treatment of 6 cycles,for the levels ofβ-EP and E2,the model group was lowest(P<0.05),the normal group was highest(P<0.05);and the high-dose group was higher than the low-dose group;For the levels of FSH and LH,the normal group was lowest(P<0.05),the model group was highest(P<0.05),and the high-dose group was lower than the low-dose group.Conclusion:Guangxi characteristic national medicine Polygala fallax Hemsl can effectively improve the levels of serum sex hormones andβ-EP in perimenopausal rat models and relieve the related symptoms with a certain dose-effect relationship.展开更多
Objective:To evaluate the antioxidant activity of extracts of dried flowers of Tithonia diversifolia(Hemsl) A.Gray(T.diversifolia) dry flower-a slirubby plant belonging to the Asteraceac family and very common in Braz...Objective:To evaluate the antioxidant activity of extracts of dried flowers of Tithonia diversifolia(Hemsl) A.Gray(T.diversifolia) dry flower-a slirubby plant belonging to the Asteraceac family and very common in Brazil,providing data to help prevent premature aging skin.Methods:The tests of phytochemical screening included total phenols,tannins,flavonoids,alkaloids and saponins.The active antioxidant was determined by 2,2-diphenyl-1-picrylhydrazyl method.Results:The phytoehemical screening of T.diversifolia dry flowers revealed the presence of phenolic compounds(tannins,flavonoids and total phenolsi,while alkaloids and saponins were not detected.The IC_(50) values showed a strong antioxidant activity of the plant extracts.Conclusions:Therefore,this study suggests the possibility of using dry flowers extracts of T.diversifolia for the prevention of cell aging,as was shown to have significant antioxidant activity.展开更多
In order to expand the production of Stevia rebaudiana( Bertoni) Hemsl.,the main points of high-yield cultivation techniques of a two-year-old stevia cultivar Shoutian No. 3 in Gannan area of Jiangxi Province were sum...In order to expand the production of Stevia rebaudiana( Bertoni) Hemsl.,the main points of high-yield cultivation techniques of a two-year-old stevia cultivar Shoutian No. 3 in Gannan area of Jiangxi Province were summarized: cutting and transplanting in early spring; fine management,applying more basic and additional fertilizers and retaining stubbles two times in summer,retaining roots for seeding in late autumn,and mulching membrane for safe overwintering in winter. This study will provide practical guidance for the planting of S. rebaudiana in Ganzhou,Jiangxi Province.展开更多
[Objectives]To establish the quality standard of Oxyria sinensis Hemsl.[Methods]The method in Chinese Pharmacopoeia(2020 edition)was used to determine the moisture,total ash,acid-insoluble ash and alcohol-soluble extr...[Objectives]To establish the quality standard of Oxyria sinensis Hemsl.[Methods]The method in Chinese Pharmacopoeia(2020 edition)was used to determine the moisture,total ash,acid-insoluble ash and alcohol-soluble extracts of O.sinensis.The qualitative identification of medicinal materials was carried out by microscopic identification method and thin layer chromatography(TLC).The contents of hyperoside and isoquercitrin were determined by high performance liquid chromatography(HPLC).[Results]The total amount of moisture,total ash,acid-insoluble ash,alcohol-soluble extract,hyperoside and isoquercitrin of 12 batches of medicinal materials were 8.56%-11.53%,11.26%-15.48%,1.62%-2.56%,17.75%-26.09%,and 0.036%-0.42%,respectively.The microscopic features were obvious,and pores,fibers,calcium oxalate clusters,calcium oxalate square crystals,cork cells could be observed.Fluorescent spots of the same color were displayed at the positions corresponding to the hyperoside reference substance.[Conclusions]Establishing relatively complete quality standards for O.sinensis medicinal materials can provide a reference for the quality control of medicinal materials.展开更多
[Objectives]To explore the effects of Polygona fallax Hemsl water extract on gastrointestinal motility in normal mice and gastric motility disorder model mice and approximate mechanism.[Methods]Using normal mice and m...[Objectives]To explore the effects of Polygona fallax Hemsl water extract on gastrointestinal motility in normal mice and gastric motility disorder model mice and approximate mechanism.[Methods]Using normal mice and mice with gastric motility disorders(modeled with atropine),the effects of different mass concentration groups of P.fallax Hemsl water extract(0.125,0.250,0.500 g/mL)and domperidone groups on gastric residual rate,small intestine propulsion rate,serum motilin(MLT),vasoactive intestinal peptide(VIP),and tissue morphology were studied.[Results]There was a highly significant difference(P<0.01)in the small intestine propulsion rate of liquid in normal mice among the different concentration groups of P.fallax Hemsl water extract compared to the blank group.The small intestine propulsion rate and gastric residue rate of semi-solid paste were statistically significant compared to the blank group(P<0.05).Among them,there was a highly significant difference between the high concentration group(67.75%±7.65%,46.5%±10.62%)and the medium concentration group(60.90%±5.87%,59.27%±7.82%)(P<0.01).There was statistical significance in normal mouse serum MLT content in the high concentration group(P<0.05).There was no effect on serum VIP levels in normal mice;no effect on the morphology of stomach and intestinal tissues of normal mice.The small intestine propulsion rate and gastric residue rate of liquid and semi-solid paste in mice with gastric motility disorders were statistically significant compared to the atropine group,with extremely significant differences(P<0.01).[Conclusions]P.fallax Hemsl water extract has a promoting effect on gastrointestinal motility.One of the specific mechanisms by which P.fallax Hemsl promotes gastrointestinal motility in normal mice may be related to the content of MLT in mouse serum.The mechanism of action in atropine induced gastric paresis mice may be related to the reactivation of M receptors,and the action mechanism of P.fallax Hemsl does not change the original histological basis.It can be inferred that P.fallax Hemsl water extract has a synergistic effect on promoting gastrointestinal motility through other mechanisms,but it is not fully understood and further in-depth research is needed.展开更多
The screening of lead compounds in plants is an important way to develop new drugs.Gentiana rhodantha Franch ex Hemsl,belonging to genus Gentiana of Gentianaceae family,has the functions of clearing heat and drying da...The screening of lead compounds in plants is an important way to develop new drugs.Gentiana rhodantha Franch ex Hemsl,belonging to genus Gentiana of Gentianaceae family,has the functions of clearing heat and drying dampness,detoxifying and purging fire,relieving cough and so on.Previous studies have shown that many compounds in G.rhodantha have antitumor activities,but the mechanism is still unclear.The purpose of this study was to investigate the anti-hepatoma activity of the compounds in G.rhodantha by using the knowledge of network pharmacology.A compound-cancer target network with 57 candidate molecules and 38 potential targets was constructed.Analysis of the models showed that the most effective compound in this plant might be compound 47(rhyncoside D)ether,and the most potential target might be GMP synthase(2vxo).We believe that the present study can provide important clues for the study of anti-hepatoma activity.展开更多
Gentiana rhodantha Franch.ex Hemsl.(G.rhodantha)is a perennial herb distributed in southwest China,belonging to the Gentiana genus of Gentianaceae family.Previous research indicated that G.rhodantha contains flavonoid...Gentiana rhodantha Franch.ex Hemsl.(G.rhodantha)is a perennial herb distributed in southwest China,belonging to the Gentiana genus of Gentianaceae family.Previous research indicated that G.rhodantha contains flavonoids,anthraquinones,terpenes and other compounds,which possess the effects of antioxidant,antibacterial,and antitumor.Besides,its dry herb is used as antiphlogistic and antitussive remedy for the treatment of lung,liver,and gallbladder diseases.This review outlines the chemical constituents and biological activities of G.rhodantha,which will be useful for the further research on this plant.展开更多
A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RAC...A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the 6. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application.展开更多
An oligosaccharide homologue named SnS-2 was isolated from the root of Scrophularia ningpoensis Hemsl. SnS-2 was purified by means of gel-permeation chromatography and ion-exchange chromatography. Its physico-chemical...An oligosaccharide homologue named SnS-2 was isolated from the root of Scrophularia ningpoensis Hemsl. SnS-2 was purified by means of gel-permeation chromatography and ion-exchange chromatography. Its physico-chemical properties, including carbohydrate content and molecular weight were determined. The structure of SnS-2 was elucidated by chemical methods along with 1H and 13C NMR spectroscopy, including two-dimensional DQCOSY and H-detected 1H, 13C HMQC experiments. These results show that SnS-2 possesses a backbone con-sisting of terminal a-Galp-(1, a-Galp-(16), a-Glcp-(16) and nonreducing end -Fruf-(2. The bioactive as-say showed that it could inhibit the growth of Lewis pulmonary carcinoma implanted in mice.展开更多
基金supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Lysimachia capillipes(L.capillipes)Hemsl,belong to primulaceae pearl vegetables genus,is a treasure in traditional Chinese medicine.It has the effects of invigorating qi and tonifying deficiency,dispelling wind and activating blood,awakening the brain,relieving cough and regulating menstruation.With the development of modern medicine,the active components and therapeutic mechanisms of L.capillipes Hemsl have been gradually revealed.The present report systematically reviews the chemical composition and biological activities of L.capillipes Hemsl,to provide scientific basis and reference for detailed research on L.capillipes Hemsl.
文摘Aim To investigate the chemical constituents of Salvia cavaleriei Levi. var. erythrophylla （Hemsl.） Stib. Methods Compounds were isolated by silica gel column chromatography and Sephadex LH-20 gel column chromatography, and the structures were identified by spectral analysis. Result Five compounds were identified as 3β, 6β, 23-trihydroxyolean-12-en-28-oic acid （1）, ursolic acid （2）, n-heptadecanoic acid （3）, n-octadecanoic acid （4）, and β-sitosterol （5）. Conclusion Five compounds were isolated from the plant for the first time.
基金National key foundation for exploring scientific ins trument of China(Grant No.2013YQ030651)National Natural Sci ence Foundation of China(Grant No.81202937)
文摘As a widely used traditional Chinese medicine （TCM）, Swertia punicea Hemsl has exhibited effects on anti-hepatitis B virus （HBV）, liver protection, hypoglycemic activity and cholecystitis. In this study, we confirmed that xanthone extract from Swertia punicea Hemsl （XSPH） improved the motor deficit, increased the levels of striatal dopamine （DA） and homovanilic acid （HVA）, and alleviated the loss of tyrosine hydroxylase （TH）-positive neurons located in substantia nigra pars compacta （SNpc） in MPTP-induced mouse model of Parkinson＇s disease （PD）. In conclusion, the present results indicated that XSPH offered neuroprotective effects against the neurotoxicity of MPTP and it might be a potential treatment for PD.
基金supported by the National Natural Science Foundation of China(No.30271590)
文摘A new compound named 1-hydrate, 3-deoxy-α-D-tagatofuranose was isolated from Swertia punicea Hemsl. The structure of compound was determined by 1D and 2D NMR, HRESIMS techniques.
文摘Two eremophilane-type sesquiterpenoids from the rhizomes of Ligularia veitchiana（Hemsl.） Greenm were isolated and determined as 10α,11-endoperoxide-1（2）-en-7α-hydroeremophilane（1） and 1（10）-en-2-oxo-7α-isopropanol-eremophilane（2）.Compound 1 was a new compound and compound 2 was a new natural product.The structures of the two compounds were established by means of spectral methods including 1^H NMR,13^C NMR,2D NMR and MS.
基金Project for Improving Basic Capabilities of Middle-aged and Young Teachers in Guangxi Institutions of Higher Learning(No.KY2016YB835)National Natural Science Foundation of China(No.81660830)+3 种基金Open Project for Guangxi Key Laboratory of Chinese Medicine Foundation Research of Guangxi(No.15-140-32-06)Open Project for Guangxi First-class Discipline Construction of Guangxi University of Chinese Medicine(No.2019XK038)Funded by Development Program of High-level Talent Team under Qihuang Project of Guangxi University of Chinese Medicine(No.2018005)Traditional Chinese Medicine-Guangxi first-class discipline construction project(No.Gui Jiao Ke Yan[2018]12).
文摘Objective:To study the effects of the ethnic medicine Polygala fallax Hemsl with Guangxi characteristics on the sex hormones andβ-EP in research objective perimenopausal rat models.Methods:40 female SPF rats were randomly divided into 4 groups,including the normal,model,high-dose and lowdose groups.Rats of three groups except for the normal one were treated with perimenopausal modelling through the method of subcutaneous injection of compound 4-VCD for 15 consecutive days.Rats of the normal and model group were normally fed without any treatment.Rats of the high-dose and low-dose groups were administered by high-and low-dose intragastric administration of the extract of Polygala fallax Hemsl.According to the menstrual cycle of the vaginal smear of the rat,each menstrual cycle is a course of treatment and 6 consecutive courses of treatment would be given.The indexes of serum sex hormones(E2,FSH,LH)andβ-EP of rats in each group were observed after treatment.Results:After the treatment of 6 cycles,for the levels ofβ-EP and E2,the model group was lowest(P<0.05),the normal group was highest(P<0.05);and the high-dose group was higher than the low-dose group;For the levels of FSH and LH,the normal group was lowest(P<0.05),the model group was highest(P<0.05),and the high-dose group was lower than the low-dose group.Conclusion:Guangxi characteristic national medicine Polygala fallax Hemsl can effectively improve the levels of serum sex hormones andβ-EP in perimenopausal rat models and relieve the related symptoms with a certain dose-effect relationship.
基金Supported by Pro-Saude Ⅱ,Fundacao do ABC(Grant No.2261/2008)
文摘Objective:To evaluate the antioxidant activity of extracts of dried flowers of Tithonia diversifolia(Hemsl) A.Gray(T.diversifolia) dry flower-a slirubby plant belonging to the Asteraceac family and very common in Brazil,providing data to help prevent premature aging skin.Methods:The tests of phytochemical screening included total phenols,tannins,flavonoids,alkaloids and saponins.The active antioxidant was determined by 2,2-diphenyl-1-picrylhydrazyl method.Results:The phytoehemical screening of T.diversifolia dry flowers revealed the presence of phenolic compounds(tannins,flavonoids and total phenolsi,while alkaloids and saponins were not detected.The IC_(50) values showed a strong antioxidant activity of the plant extracts.Conclusions:Therefore,this study suggests the possibility of using dry flowers extracts of T.diversifolia for the prevention of cell aging,as was shown to have significant antioxidant activity.
基金Supported by Science and Technology Project of Education Department of Jiangxi Province(GJJ161243)"11531" Project of Nanchang Normal University
文摘In order to expand the production of Stevia rebaudiana( Bertoni) Hemsl.,the main points of high-yield cultivation techniques of a two-year-old stevia cultivar Shoutian No. 3 in Gannan area of Jiangxi Province were summarized: cutting and transplanting in early spring; fine management,applying more basic and additional fertilizers and retaining stubbles two times in summer,retaining roots for seeding in late autumn,and mulching membrane for safe overwintering in winter. This study will provide practical guidance for the planting of S. rebaudiana in Ganzhou,Jiangxi Province.
基金Supported by Chinese Medicine(Ethnic Medicine)Standard Improvement Project of Sichuan Medical Products Administration(510201202102305)School Level Innovation Team of Fundamental Research Funds for the Central Universities(ZYN2022067)。
文摘[Objectives]To establish the quality standard of Oxyria sinensis Hemsl.[Methods]The method in Chinese Pharmacopoeia(2020 edition)was used to determine the moisture,total ash,acid-insoluble ash and alcohol-soluble extracts of O.sinensis.The qualitative identification of medicinal materials was carried out by microscopic identification method and thin layer chromatography(TLC).The contents of hyperoside and isoquercitrin were determined by high performance liquid chromatography(HPLC).[Results]The total amount of moisture,total ash,acid-insoluble ash,alcohol-soluble extract,hyperoside and isoquercitrin of 12 batches of medicinal materials were 8.56%-11.53%,11.26%-15.48%,1.62%-2.56%,17.75%-26.09%,and 0.036%-0.42%,respectively.The microscopic features were obvious,and pores,fibers,calcium oxalate clusters,calcium oxalate square crystals,cork cells could be observed.Fluorescent spots of the same color were displayed at the positions corresponding to the hyperoside reference substance.[Conclusions]Establishing relatively complete quality standards for O.sinensis medicinal materials can provide a reference for the quality control of medicinal materials.
基金2022 National College Student Innovation and Entrepreneurship Training Program(202210599004).
文摘[Objectives]To explore the effects of Polygona fallax Hemsl water extract on gastrointestinal motility in normal mice and gastric motility disorder model mice and approximate mechanism.[Methods]Using normal mice and mice with gastric motility disorders(modeled with atropine),the effects of different mass concentration groups of P.fallax Hemsl water extract(0.125,0.250,0.500 g/mL)and domperidone groups on gastric residual rate,small intestine propulsion rate,serum motilin(MLT),vasoactive intestinal peptide(VIP),and tissue morphology were studied.[Results]There was a highly significant difference(P<0.01)in the small intestine propulsion rate of liquid in normal mice among the different concentration groups of P.fallax Hemsl water extract compared to the blank group.The small intestine propulsion rate and gastric residue rate of semi-solid paste were statistically significant compared to the blank group(P<0.05).Among them,there was a highly significant difference between the high concentration group(67.75%±7.65%,46.5%±10.62%)and the medium concentration group(60.90%±5.87%,59.27%±7.82%)(P<0.01).There was statistical significance in normal mouse serum MLT content in the high concentration group(P<0.05).There was no effect on serum VIP levels in normal mice;no effect on the morphology of stomach and intestinal tissues of normal mice.The small intestine propulsion rate and gastric residue rate of liquid and semi-solid paste in mice with gastric motility disorders were statistically significant compared to the atropine group,with extremely significant differences(P<0.01).[Conclusions]P.fallax Hemsl water extract has a promoting effect on gastrointestinal motility.One of the specific mechanisms by which P.fallax Hemsl promotes gastrointestinal motility in normal mice may be related to the content of MLT in mouse serum.The mechanism of action in atropine induced gastric paresis mice may be related to the reactivation of M receptors,and the action mechanism of P.fallax Hemsl does not change the original histological basis.It can be inferred that P.fallax Hemsl water extract has a synergistic effect on promoting gastrointestinal motility through other mechanisms,but it is not fully understood and further in-depth research is needed.
文摘The screening of lead compounds in plants is an important way to develop new drugs.Gentiana rhodantha Franch ex Hemsl,belonging to genus Gentiana of Gentianaceae family,has the functions of clearing heat and drying dampness,detoxifying and purging fire,relieving cough and so on.Previous studies have shown that many compounds in G.rhodantha have antitumor activities,but the mechanism is still unclear.The purpose of this study was to investigate the anti-hepatoma activity of the compounds in G.rhodantha by using the knowledge of network pharmacology.A compound-cancer target network with 57 candidate molecules and 38 potential targets was constructed.Analysis of the models showed that the most effective compound in this plant might be compound 47(rhyncoside D)ether,and the most potential target might be GMP synthase(2vxo).We believe that the present study can provide important clues for the study of anti-hepatoma activity.
文摘Gentiana rhodantha Franch.ex Hemsl.(G.rhodantha)is a perennial herb distributed in southwest China,belonging to the Gentiana genus of Gentianaceae family.Previous research indicated that G.rhodantha contains flavonoids,anthraquinones,terpenes and other compounds,which possess the effects of antioxidant,antibacterial,and antitumor.Besides,its dry herb is used as antiphlogistic and antitussive remedy for the treatment of lung,liver,and gallbladder diseases.This review outlines the chemical constituents and biological activities of G.rhodantha,which will be useful for the further research on this plant.
文摘A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the 6. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application.
文摘An oligosaccharide homologue named SnS-2 was isolated from the root of Scrophularia ningpoensis Hemsl. SnS-2 was purified by means of gel-permeation chromatography and ion-exchange chromatography. Its physico-chemical properties, including carbohydrate content and molecular weight were determined. The structure of SnS-2 was elucidated by chemical methods along with 1H and 13C NMR spectroscopy, including two-dimensional DQCOSY and H-detected 1H, 13C HMQC experiments. These results show that SnS-2 possesses a backbone con-sisting of terminal a-Galp-(1, a-Galp-(16), a-Glcp-(16) and nonreducing end -Fruf-(2. The bioactive as-say showed that it could inhibit the growth of Lewis pulmonary carcinoma implanted in mice.
