The effects of recombinant galectins of male and female Hemonchus contortus （Hco-gal-m/f） on the mRNA levels of IL-1β, IL-2, IL-4, IL-6, interferon-γ（IFN-γ）, and tumor necrosis factor-γ （TNF-γ） of goat peri...The effects of recombinant galectins of male and female Hemonchus contortus （Hco-gal-m/f） on the mRNA levels of IL-1β, IL-2, IL-4, IL-6, interferon-γ（IFN-γ）, and tumor necrosis factor-γ （TNF-γ） of goat peripheral blood mononuclear cells （PBMCs） were examined in this study. Blood samples were collected from five randomly selected two-year-old healthy goats. PBMCs were separated and cultured in vitro with varying concentration galectins （Hco-gal-m/f） of 0, 10, 20, and 40 lag mL-L Semi-quantitative reverse transcription RT-PCR was employed to test the synthesis of mRNA. The results showed that the mRNA transcriptions of IL-1β, IL-2, IL-4, IL-6, IFN-γ, and TNF-γ in the PBMCs were inhibited by Hco-galm in a dose-dependent manner. Hco-gal-f inhibited mRNA synthesis of IL- 1β, IL-4, IFN-γ, and TNF-γ of the same cells in similar fashion. The findings suggested that the recombinant galectin proteins of H. contortus could decrease the transcription of cytokines in vitro.展开更多
基金the National Natural Science Foundation of China （30071078）.
文摘The effects of recombinant galectins of male and female Hemonchus contortus （Hco-gal-m/f） on the mRNA levels of IL-1β, IL-2, IL-4, IL-6, interferon-γ（IFN-γ）, and tumor necrosis factor-γ （TNF-γ） of goat peripheral blood mononuclear cells （PBMCs） were examined in this study. Blood samples were collected from five randomly selected two-year-old healthy goats. PBMCs were separated and cultured in vitro with varying concentration galectins （Hco-gal-m/f） of 0, 10, 20, and 40 lag mL-L Semi-quantitative reverse transcription RT-PCR was employed to test the synthesis of mRNA. The results showed that the mRNA transcriptions of IL-1β, IL-2, IL-4, IL-6, IFN-γ, and TNF-γ in the PBMCs were inhibited by Hco-galm in a dose-dependent manner. Hco-gal-f inhibited mRNA synthesis of IL- 1β, IL-4, IFN-γ, and TNF-γ of the same cells in similar fashion. The findings suggested that the recombinant galectin proteins of H. contortus could decrease the transcription of cytokines in vitro.
