数控磨刀机是一种高效率的刀具磨削加工机床,用于大部分形状的金属切削刀具的加工、开刃和修磨。德国瓦尔特HELI46六坐标数控磨刀机数控系统HMC-500为车间进行刀具的柔性制造提供人机交互及数控轴插补。但由于其多总线的数控系统控制结...数控磨刀机是一种高效率的刀具磨削加工机床,用于大部分形状的金属切削刀具的加工、开刃和修磨。德国瓦尔特HELI46六坐标数控磨刀机数控系统HMC-500为车间进行刀具的柔性制造提供人机交互及数控轴插补。但由于其多总线的数控系统控制结构,设备维护人员对磨刀机在加工过程中的异常及故障的处置也比较棘手。因此,通过对一台HELI46数控磨刀机P L C典型故障的分析及维修,提出了一种实用高效的现场问题处理方法,并将其固化为业务流程,可以高效对磨刀机异常情况及故障进行处理,并提高磨刀机利用率。展开更多
基于非定常面元/时间步进全展自由尾迹建立了旋翼/机身非定常气动干扰分析方法。方法中耦合了非定常面元法和时间步进自由尾迹,以准确模拟旋翼非定常气动力、旋翼尾迹及桨叶对机身的非定常干扰效应。为模拟前飞状态下具有升力的机身,将...基于非定常面元/时间步进全展自由尾迹建立了旋翼/机身非定常气动干扰分析方法。方法中耦合了非定常面元法和时间步进自由尾迹,以准确模拟旋翼非定常气动力、旋翼尾迹及桨叶对机身的非定常干扰效应。为模拟前飞状态下具有升力的机身,将机身离散为汇/偶极子面元,并采用涡线镜像法模拟旋翼尾迹靠近机身表面产生的加速效应。通过计算前飞状态的Maryland、ROBIN(Rotor Body INteraction)旋翼/机身干扰下的非定常压力分布,并与可得到的实验值、CFD计算结果对比,验证方法的准确性。随后分析前飞速度、旋翼与机身距离对旋翼/机身非定气动干扰的影响。计算结果表明机身头部和中部非定常压力主要受桨叶的通过性影响,而机身尾梁主要受尾迹/机身干扰影响,机身非定常气动力频率为桨叶片数的倍频。随前飞速度的增加,机身非定常压力幅值增加,尾梁压力幅值先增加后减小;增加旋翼与机身距离将减小机身和尾梁非定常压力幅值。展开更多
Gastric cancer(GC)is the fourth most common cancer in the world and the second cause of cancer-related death.Gastric carcinogenesis is a multifactorial process,in which environmental and genetic factors interact to ac...Gastric cancer(GC)is the fourth most common cancer in the world and the second cause of cancer-related death.Gastric carcinogenesis is a multifactorial process,in which environmental and genetic factors interact to activate multiple intracellular signals thus leading to uncontrolled growth and survival of GC cells.One such a pathway is regulated by proteinase activated-receptors(PARs),seven transmembrane-spanning domain G protein-coupled receptors,which comprise four receptors(i.e.,PAR-1,PAR-2,PAR-3,and PAR-4)activated by various proteases.Both PAR-1 and PAR-2 are over-expressed on GC cells and their activation triggers and/or amplifies intracellular pathways,which sustain gastric carcinogenesis.There is also evidence that expression of either PAR-1 or PAR-2 correlates with depth of wall invasion and metastatic dissemination and inversely with the overall survival of patients.Consistently,data emerging from experimental models of GC suggest that both these receptors can be important targets for therapeutic interventions in GC patients.In contrast,PAR-4levels are down-regulated in GC and correlate inversely with the aggressiveness of GC,thus suggesting a negative role of this receptor in the control of GC.In this article we review the available data on the expression and role of PARs in GC and discuss whether manipulation of PAR-driven signals may be useful for interfering with GC cell behavior.展开更多
AIM: To explore methylation of DAPK, THBS1, CDH-1, and p14 genes, and Helicobacter pylori(H. pylori) status in individuals harboring esophageal columnar metaplasia.METHODS: Distal esophageal mucosal samples obtained b...AIM: To explore methylation of DAPK, THBS1, CDH-1, and p14 genes, and Helicobacter pylori(H. pylori) status in individuals harboring esophageal columnar metaplasia.METHODS: Distal esophageal mucosal samples obtained by endoscopy and histologically diagnosed as gastric-type(non-specialized) columnar metaplasia, were studied thoroughly. DNA was extracted from paraffin blocks, and methylation status of deathassociated protein kinase(DAPK), thrombospondin-1(THBS1), cadherin-1(CDH1), and p14 genes, was examined using a methyl-sensitive polymerase chain reaction(MS-PCR) and sodium bisulfite modification protocol. H. pylori cag A status was determined by PCR.RESULTS: In total, 68 subjects(33 females and 35 males), with a mean age of 52 years, were included. H. pylori cag A positive was present in the esophageal gastric-type metaplastic mucosa of 18 individuals. DAPK, THSB1, CDH1, and p14 gene promoters were methylated by MS-PCR in 40(58.8%), 33(48.5%), 46(67.6%), and 23(33.8%) cases of the 68 esophageal samples. H. pyloristatus was associated with methylation of DAPK(P = 0.003) and THBS1(P = 0.019).CONCLUSION: DNA methylation occurs in cases of gastric-type(non-specialized) columnar metaplasia of the esophagus, and this modification is associated with H. pylori cag A positive infection.展开更多
文摘数控磨刀机是一种高效率的刀具磨削加工机床,用于大部分形状的金属切削刀具的加工、开刃和修磨。德国瓦尔特HELI46六坐标数控磨刀机数控系统HMC-500为车间进行刀具的柔性制造提供人机交互及数控轴插补。但由于其多总线的数控系统控制结构,设备维护人员对磨刀机在加工过程中的异常及故障的处置也比较棘手。因此,通过对一台HELI46数控磨刀机P L C典型故障的分析及维修,提出了一种实用高效的现场问题处理方法,并将其固化为业务流程,可以高效对磨刀机异常情况及故障进行处理,并提高磨刀机利用率。
文摘基于非定常面元/时间步进全展自由尾迹建立了旋翼/机身非定常气动干扰分析方法。方法中耦合了非定常面元法和时间步进自由尾迹,以准确模拟旋翼非定常气动力、旋翼尾迹及桨叶对机身的非定常干扰效应。为模拟前飞状态下具有升力的机身,将机身离散为汇/偶极子面元,并采用涡线镜像法模拟旋翼尾迹靠近机身表面产生的加速效应。通过计算前飞状态的Maryland、ROBIN(Rotor Body INteraction)旋翼/机身干扰下的非定常压力分布,并与可得到的实验值、CFD计算结果对比,验证方法的准确性。随后分析前飞速度、旋翼与机身距离对旋翼/机身非定气动干扰的影响。计算结果表明机身头部和中部非定常压力主要受桨叶的通过性影响,而机身尾梁主要受尾迹/机身干扰影响,机身非定常气动力频率为桨叶片数的倍频。随前飞速度的增加,机身非定常压力幅值增加,尾梁压力幅值先增加后减小;增加旋翼与机身距离将减小机身和尾梁非定常压力幅值。
文摘Gastric cancer(GC)is the fourth most common cancer in the world and the second cause of cancer-related death.Gastric carcinogenesis is a multifactorial process,in which environmental and genetic factors interact to activate multiple intracellular signals thus leading to uncontrolled growth and survival of GC cells.One such a pathway is regulated by proteinase activated-receptors(PARs),seven transmembrane-spanning domain G protein-coupled receptors,which comprise four receptors(i.e.,PAR-1,PAR-2,PAR-3,and PAR-4)activated by various proteases.Both PAR-1 and PAR-2 are over-expressed on GC cells and their activation triggers and/or amplifies intracellular pathways,which sustain gastric carcinogenesis.There is also evidence that expression of either PAR-1 or PAR-2 correlates with depth of wall invasion and metastatic dissemination and inversely with the overall survival of patients.Consistently,data emerging from experimental models of GC suggest that both these receptors can be important targets for therapeutic interventions in GC patients.In contrast,PAR-4levels are down-regulated in GC and correlate inversely with the aggressiveness of GC,thus suggesting a negative role of this receptor in the control of GC.In this article we review the available data on the expression and role of PARs in GC and discuss whether manipulation of PAR-driven signals may be useful for interfering with GC cell behavior.
文摘AIM: To explore methylation of DAPK, THBS1, CDH-1, and p14 genes, and Helicobacter pylori(H. pylori) status in individuals harboring esophageal columnar metaplasia.METHODS: Distal esophageal mucosal samples obtained by endoscopy and histologically diagnosed as gastric-type(non-specialized) columnar metaplasia, were studied thoroughly. DNA was extracted from paraffin blocks, and methylation status of deathassociated protein kinase(DAPK), thrombospondin-1(THBS1), cadherin-1(CDH1), and p14 genes, was examined using a methyl-sensitive polymerase chain reaction(MS-PCR) and sodium bisulfite modification protocol. H. pylori cag A status was determined by PCR.RESULTS: In total, 68 subjects(33 females and 35 males), with a mean age of 52 years, were included. H. pylori cag A positive was present in the esophageal gastric-type metaplastic mucosa of 18 individuals. DAPK, THSB1, CDH1, and p14 gene promoters were methylated by MS-PCR in 40(58.8%), 33(48.5%), 46(67.6%), and 23(33.8%) cases of the 68 esophageal samples. H. pyloristatus was associated with methylation of DAPK(P = 0.003) and THBS1(P = 0.019).CONCLUSION: DNA methylation occurs in cases of gastric-type(non-specialized) columnar metaplasia of the esophagus, and this modification is associated with H. pylori cag A positive infection.
