目的探究壳聚糖-普鲁士蓝纳米酶(chitosan-prussian blue nanozyme,CS-PB)对过氧化氢诱导的小鼠耳蜗毛细胞(house ear institute-organ of corti 1,HEI-OC1)氧化应激损伤的影响及相关分子机制.方法体外培养HEI-OC1细胞,不同浓度过氧化...目的探究壳聚糖-普鲁士蓝纳米酶(chitosan-prussian blue nanozyme,CS-PB)对过氧化氢诱导的小鼠耳蜗毛细胞(house ear institute-organ of corti 1,HEI-OC1)氧化应激损伤的影响及相关分子机制.方法体外培养HEI-OC1细胞,不同浓度过氧化氢处理细胞后通过细胞计数试剂盒-8检测细胞活性,建立过氧化氢应激损伤细胞模型,分为对照组、过氧化氢组、过氧化氢+CS-PB组、过氧化氢+N-乙酰半胱氨酸(N-Acetylcysteine,NAC)组,利用DCFH-DA荧光探针技术检测HEI-OC1细胞活性氧的含量,Western Blot检测HEI-OC1细胞肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、Bcl-XL、Bax蛋白表达水平;免疫荧光染色及激光共聚焦显微镜成像检测HEI-OC1细胞中4-HNE含量.结果与对照组相比,过氧化氢组细胞活力下降(P<0.0001),Bcl-XL蛋白表达下降,细胞内活性氧、4-HNE、TNF-α、IL-1β、Bax表达升高(P<0.0001);与过氧化氢组比较,过氧化氢+CS-PB组、过氧化氢+NAC组细胞活力升高(P<0.05),Bcl-XL蛋白表达升高,细胞内活性氧、4-HNE、TNF-α、IL-1β、Bax表达降低(P<0.01).结论CS-PB能够缓解过氧化氢诱导的HEI-OC1细胞氧化应激所造成的细胞损伤及凋亡.展开更多
Gas-phase HeI ultraviolet photoelectron spectra of m-dicyanobenzelle, P dicyanobenzene and 1,2,4,5-tetracyanobenzene are given in this paper. Photoelectronspectra are assigned using MNDO molecular orbital calculation ...Gas-phase HeI ultraviolet photoelectron spectra of m-dicyanobenzelle, P dicyanobenzene and 1,2,4,5-tetracyanobenzene are given in this paper. Photoelectronspectra are assigned using MNDO molecular orbital calculation and correlation analysisfor benzene and each cyanobenzene. It may be pointed out that there is some effect of thesubstituted position and numbers of -CN group on the relative ordering levels, splittingsand the levels of the two orbitals splitted by elg(π) of benzene.展开更多
文摘Gas-phase HeI ultraviolet photoelectron spectra of m-dicyanobenzelle, P dicyanobenzene and 1,2,4,5-tetracyanobenzene are given in this paper. Photoelectronspectra are assigned using MNDO molecular orbital calculation and correlation analysisfor benzene and each cyanobenzene. It may be pointed out that there is some effect of thesubstituted position and numbers of -CN group on the relative ordering levels, splittingsand the levels of the two orbitals splitted by elg(π) of benzene.
