Objective:To characterize the effects of Qingwei San(QWS)on diabetic oral ulcer(OU)mice with stomach heat pattern through metabolomic analysis.Methods:A stomach heat pattern mouse model was established by treating C57...Objective:To characterize the effects of Qingwei San(QWS)on diabetic oral ulcer(OU)mice with stomach heat pattern through metabolomic analysis.Methods:A stomach heat pattern mouse model was established by treating C57BLKS/J Leprdb/db(db/db)mice with dried Zingiber officinale Rosc.rhizome(Z.officinale,Gan Jiang)decoction by gavage.All model mice had blood glucose levels of≥11.1 m M.Subsequently,OU was induced by Na OH cauterization.After 1 week of administration of QWS,non-targeted metabolomic analysis of serum was conducted using ultra high performance liquid chromatography coupled with mass spectrometry(UHPLC-MS/MS).Results:The non-targeted metabolomics results indicated that tryptophan metabolism,2-oxocarboxylic acid metabolism,serotonergic synapses,amino sugar and nucleotide sugar metabolism,and amino acid biosynthesis were involved in the therapeutic effects of QWS,with tryptophan metabolism playing a predominant role.Conclusion:QWS treatment can significantly improve the pathological status of diabetic OU mice with stomach heat pattern.QWS may regulate the release of inflammatory factors through the tryptophan metabolism pathway.展开更多
Objective： To examine the association of body shape with cold and heat patterns, to determine which anthropometric measure is the best indicator for discriminating between the two patterns, and to investigate whether...Objective： To examine the association of body shape with cold and heat patterns, to determine which anthropometric measure is the best indicator for discriminating between the two patterns, and to investigate whether using a combination of measures can improve the predictive power to diagnose these patterns. Methods： Based on a total of 4,859 subjects （3,000 women and 1,859 men）, statistical analyses using binary logistic regression were performed to assess the significance of the difference and the predictive power of each anthropometric measure, and binary logistic regression and Naive Bayes with the variable selection technique were used to assess the improvement in the predictive power of the patterns using the combined measures. Results： In women, the strongest indicators for determining the cold and heat patterns among anthropometric measures were body mass index （BMI） and rib circumference; in men, the best indicator was BMI. In experiments using a combination of measures, the values of the area under the receiver operating characteristic curve in women were 0.776 by Naive Bayes and 0.772 by logistic regression, and the values in men were 0.788 by Naive Bayes and 0.779 by logistic regression. Conclusions： Individuals with a higher BMI have a tendency toward a heat pattern in both women and men. The use of a combination of anthropometric measures can slightly improve the diagnostic accuracy. Our findings can provide fundamental information for the diagnosis of cold and heat patterns based on body shape for personalized medicine.展开更多
Microwave ablation(MWA)is a cancer treatment method.The tumor tissue absorbs electromagnetic energy,which heats and kills it.A microwave ablation antenna plays a critical role in this process.Its radiation field must ...Microwave ablation(MWA)is a cancer treatment method.The tumor tissue absorbs electromagnetic energy,which heats and kills it.A microwave ablation antenna plays a critical role in this process.Its radiation field must completely cover the tumor but not the healthy tissue.At present,the radiation pattern of most invasive ablation antennas is spherical.However,in the clinic,the shape of some tumors may be asymmetrical or the antenna cannot be inserted into the center of the tumor for some other reason.In order to solve these problems,a directional heating antenna for microwave ablation is proposed in this paper.The proposed antenna,operating at 2.45 GHz,consists of a monopole and a reflector.The feed is given by a substrate integrated coaxial line(SICL)and coplanar waveguide(CPW).The omnidirectional radiation field of the monopole is reflected by a reflector that is extended from the outer conductors of the SICL to form a directional radiation field.The impedance matching network is designed on SICL to match the antenna to 50Ω.The antenna is fabricated using a mature printed circuit board(PCB).The reflection coefficient of the antenna in porcine liver tissue measured by a vector network analyzer shows good agreement with the simulations.Then,an ablation experiment in porcine liver is conducted with power of 10 W for 10 min,and the experimental results confirm the validity of the design.展开更多
The heat flux deposition pattern on the toroidal limiters installed in HT-7 was simulated with ANSYS code. The simulation model was established with the ripple of the magnetic field. The heat deposition pattern and te...The heat flux deposition pattern on the toroidal limiters installed in HT-7 was simulated with ANSYS code. The simulation model was established with the ripple of the magnetic field. The heat deposition pattern and temperature distribution on the surface of the toroidal linfiters were obtained. A comparison of the results obtained with and without the shaped tiles, used to reduce the heat flux on the leading edge of the limiters, was made. The maximum heat load allowed at the leading edge was about 1.8 MW/m2 because of the poor power removing capacity on the ends of the limiters. This approach can also be applied to other devices with a limiter configuration in a circular cross-section shape.展开更多
This paper analyzes the sources of heat losses in magnetic fluid bearings,proposes various cou-pling relationships of physical fields,divides the coupled heat transfer surfaces while ensuring the continuity of heat fl...This paper analyzes the sources of heat losses in magnetic fluid bearings,proposes various cou-pling relationships of physical fields,divides the coupled heat transfer surfaces while ensuring the continuity of heat flux density,and analyzes the overall heat dissipation pathways of the bearings.By changing parameters such as input current,rotor speed,and inlet oil flow rate,the study applies a multi-physics field coupling method to investigate the influence of different parameters on the temper-ature field and heat dissipation patterns of the bearings,which is then validated through experi-ments.This research provides a theoretical basis for the optimal design of magnetic fluid bearing sys-tems.展开更多
A pseudoplastic metal nanoparticle fluid （PMNF） is used in nanoimprint to fabricate semiconductors and func- tional devices. The evaporation of the solvent and the sintering of the Au PMNF are investigated. The key ...A pseudoplastic metal nanoparticle fluid （PMNF） is used in nanoimprint to fabricate semiconductors and func- tional devices. The evaporation of the solvent and the sintering of the Au PMNF are investigated. The key parameters, which influence the morphology of patterning, such as the radius of metal particles, the concentra- tion of metal particles, the Hamaker constant of the solvent, viscosity of the fluids and the evaporation velocity, are analyzed. Based on a two-sphere sintering model, the equations are derived, which represent the relationships between the relative shrinkage and radius of the metal particles, sintering temperature and time. The optimal parameters for the heat treatment are provided in nanoimprint.展开更多
脱水响应元件结合蛋白2(dehydration responsive element binding protein 2,DREB2)是一种转录因子,在模式植物应答干旱、高盐和热激胁迫中起调控作用。然而,在适应喀斯特干热石山环境的文采报春苣苔(Primulina wentsaii)中,这些基因的...脱水响应元件结合蛋白2(dehydration responsive element binding protein 2,DREB2)是一种转录因子,在模式植物应答干旱、高盐和热激胁迫中起调控作用。然而,在适应喀斯特干热石山环境的文采报春苣苔(Primulina wentsaii)中,这些基因的功能尚不清楚。筛选对干热双重胁迫联合响应的文采报春苣苔DREB2同源基因,可为苦苣苔抗逆种质的创制提供新的基因储备。该研究首先根据文采报春苣苔转录组的序列信息设计特异引物,以gDNA和cDNA为模板分离PwDREB2s基因,然后通过生物信息学方法,对氨基酸序列、系统进化关系及保守基序进行分析,再使用半定量RT-PCR(sqRT-PCR)分析PwDREB2s基因在低温、热激、模拟干旱(渗透)、高盐、外源ABA及氧化等单一胁迫下的表达模式,最后选择能同时应答模拟干旱与热激的成员,利用实时荧光定量PCR(qRT-PCR)检测其在单一和复合干热处理中的转录本水平。结果表明:(1)分离获得了8条缺少内含子的PwDREB2s基因,编码198~386个氨基酸,拥有AP2/ERF结构域、核定位信号或转录激活域等典型特征序列;(2)PwDREB2A/2AL1/2AL2、PwDREB2D/2DL和PwDREB2F转录因子被分别归入A-2亚组的亚型1、亚型2和亚型3,PwDREB2EL1/2EL2转录因子则被归入A-6亚组;(3)SqRT-PCR分析表明PwDREB2s基因表达受多种单一胁迫的诱导,其中PwDREB2A/2AL1/2AL2/2D基因能同时应答模拟干旱与热激胁迫;(4)qRT-PCR结果揭示了在液体培养条件下,PwDREB2D基因对干热处理的应答具有组织特异性,仅能在根状茎中被强烈诱导。在土壤栽培条件下,与对照相比时,除PwDREB2D基因以外,PwDREB2A/2AL1/2AL2基因在自然干旱-热激复合胁迫中的转录反应显著增强。与单一的自然干旱和高温处理相比时,PwDREB2AL1/2AL2基因在自然干旱-热激复合胁迫中的表达水平显著升高。由此可见,PwDREB2AL1/2AL2基因在文采报春苣苔干热复合胁迫应答中可能发挥了重要的调控作用。展开更多
基金funded by the Science and Technology Development Fund,Macao,China(007/2020/ALC)Major Basic and Applied Basic Research Projects of Guangdong Province of China(2019B030302005)the National Natural Science Foundation of China(81973535 and 81773960)。
文摘Objective:To characterize the effects of Qingwei San(QWS)on diabetic oral ulcer(OU)mice with stomach heat pattern through metabolomic analysis.Methods:A stomach heat pattern mouse model was established by treating C57BLKS/J Leprdb/db(db/db)mice with dried Zingiber officinale Rosc.rhizome(Z.officinale,Gan Jiang)decoction by gavage.All model mice had blood glucose levels of≥11.1 m M.Subsequently,OU was induced by Na OH cauterization.After 1 week of administration of QWS,non-targeted metabolomic analysis of serum was conducted using ultra high performance liquid chromatography coupled with mass spectrometry(UHPLC-MS/MS).Results:The non-targeted metabolomics results indicated that tryptophan metabolism,2-oxocarboxylic acid metabolism,serotonergic synapses,amino sugar and nucleotide sugar metabolism,and amino acid biosynthesis were involved in the therapeutic effects of QWS,with tryptophan metabolism playing a predominant role.Conclusion:QWS treatment can significantly improve the pathological status of diabetic OU mice with stomach heat pattern.QWS may regulate the release of inflammatory factors through the tryptophan metabolism pathway.
基金Supported by the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT&Future Planning(No.2006-2005173,NRF-2012-0009830,and NRF-2009-0090900)by the Bio&Medical Technology Development Program of the NRF funded by the Korean government,MSIP(No.NRF2015M3A9B6027139)
文摘Objective： To examine the association of body shape with cold and heat patterns, to determine which anthropometric measure is the best indicator for discriminating between the two patterns, and to investigate whether using a combination of measures can improve the predictive power to diagnose these patterns. Methods： Based on a total of 4,859 subjects （3,000 women and 1,859 men）, statistical analyses using binary logistic regression were performed to assess the significance of the difference and the predictive power of each anthropometric measure, and binary logistic regression and Naive Bayes with the variable selection technique were used to assess the improvement in the predictive power of the patterns using the combined measures. Results： In women, the strongest indicators for determining the cold and heat patterns among anthropometric measures were body mass index （BMI） and rib circumference; in men, the best indicator was BMI. In experiments using a combination of measures, the values of the area under the receiver operating characteristic curve in women were 0.776 by Naive Bayes and 0.772 by logistic regression, and the values in men were 0.788 by Naive Bayes and 0.779 by logistic regression. Conclusions： Individuals with a higher BMI have a tendency toward a heat pattern in both women and men. The use of a combination of anthropometric measures can slightly improve the diagnostic accuracy. Our findings can provide fundamental information for the diagnosis of cold and heat patterns based on body shape for personalized medicine.
基金Project supported by the National Natural Science Foundation of China(Grant No.U1966201)the Fundamental Research Funds for the Central Universities,China(Grant No.A03019023801224)。
文摘Microwave ablation(MWA)is a cancer treatment method.The tumor tissue absorbs electromagnetic energy,which heats and kills it.A microwave ablation antenna plays a critical role in this process.Its radiation field must completely cover the tumor but not the healthy tissue.At present,the radiation pattern of most invasive ablation antennas is spherical.However,in the clinic,the shape of some tumors may be asymmetrical or the antenna cannot be inserted into the center of the tumor for some other reason.In order to solve these problems,a directional heating antenna for microwave ablation is proposed in this paper.The proposed antenna,operating at 2.45 GHz,consists of a monopole and a reflector.The feed is given by a substrate integrated coaxial line(SICL)and coplanar waveguide(CPW).The omnidirectional radiation field of the monopole is reflected by a reflector that is extended from the outer conductors of the SICL to form a directional radiation field.The impedance matching network is designed on SICL to match the antenna to 50Ω.The antenna is fabricated using a mature printed circuit board(PCB).The reflection coefficient of the antenna in porcine liver tissue measured by a vector network analyzer shows good agreement with the simulations.Then,an ablation experiment in porcine liver is conducted with power of 10 W for 10 min,and the experimental results confirm the validity of the design.
基金National Natural Science Foundation of China(No.10475080)the Foundation of Hefei Institutes of Physical Science(No.2006YZJJ-1)
文摘The heat flux deposition pattern on the toroidal limiters installed in HT-7 was simulated with ANSYS code. The simulation model was established with the ripple of the magnetic field. The heat deposition pattern and temperature distribution on the surface of the toroidal linfiters were obtained. A comparison of the results obtained with and without the shaped tiles, used to reduce the heat flux on the leading edge of the limiters, was made. The maximum heat load allowed at the leading edge was about 1.8 MW/m2 because of the poor power removing capacity on the ends of the limiters. This approach can also be applied to other devices with a limiter configuration in a circular cross-section shape.
基金the National Natural Science Foundation of China(No.52075468)the Natural Science Foundation of Hebei Province(No.E2020203052)+1 种基金the Key Scientific Research Projects of North China University of Technology(No.ZD-YG-202306-23)the Tangshan Science and Technology Project(No.23130201E).
文摘This paper analyzes the sources of heat losses in magnetic fluid bearings,proposes various cou-pling relationships of physical fields,divides the coupled heat transfer surfaces while ensuring the continuity of heat flux density,and analyzes the overall heat dissipation pathways of the bearings.By changing parameters such as input current,rotor speed,and inlet oil flow rate,the study applies a multi-physics field coupling method to investigate the influence of different parameters on the temper-ature field and heat dissipation patterns of the bearings,which is then validated through experi-ments.This research provides a theoretical basis for the optimal design of magnetic fluid bearing sys-tems.
基金Supported by the National Natural Science Foundation of China under Grant Nos 51175479 and 51475436the Education Department of Henan Province under Grant Nos 13A460725 and 2013GGJS-001
文摘A pseudoplastic metal nanoparticle fluid （PMNF） is used in nanoimprint to fabricate semiconductors and func- tional devices. The evaporation of the solvent and the sintering of the Au PMNF are investigated. The key parameters, which influence the morphology of patterning, such as the radius of metal particles, the concentra- tion of metal particles, the Hamaker constant of the solvent, viscosity of the fluids and the evaporation velocity, are analyzed. Based on a two-sphere sintering model, the equations are derived, which represent the relationships between the relative shrinkage and radius of the metal particles, sintering temperature and time. The optimal parameters for the heat treatment are provided in nanoimprint.
文摘脱水响应元件结合蛋白2(dehydration responsive element binding protein 2,DREB2)是一种转录因子,在模式植物应答干旱、高盐和热激胁迫中起调控作用。然而,在适应喀斯特干热石山环境的文采报春苣苔(Primulina wentsaii)中,这些基因的功能尚不清楚。筛选对干热双重胁迫联合响应的文采报春苣苔DREB2同源基因,可为苦苣苔抗逆种质的创制提供新的基因储备。该研究首先根据文采报春苣苔转录组的序列信息设计特异引物,以gDNA和cDNA为模板分离PwDREB2s基因,然后通过生物信息学方法,对氨基酸序列、系统进化关系及保守基序进行分析,再使用半定量RT-PCR(sqRT-PCR)分析PwDREB2s基因在低温、热激、模拟干旱(渗透)、高盐、外源ABA及氧化等单一胁迫下的表达模式,最后选择能同时应答模拟干旱与热激的成员,利用实时荧光定量PCR(qRT-PCR)检测其在单一和复合干热处理中的转录本水平。结果表明:(1)分离获得了8条缺少内含子的PwDREB2s基因,编码198~386个氨基酸,拥有AP2/ERF结构域、核定位信号或转录激活域等典型特征序列;(2)PwDREB2A/2AL1/2AL2、PwDREB2D/2DL和PwDREB2F转录因子被分别归入A-2亚组的亚型1、亚型2和亚型3,PwDREB2EL1/2EL2转录因子则被归入A-6亚组;(3)SqRT-PCR分析表明PwDREB2s基因表达受多种单一胁迫的诱导,其中PwDREB2A/2AL1/2AL2/2D基因能同时应答模拟干旱与热激胁迫;(4)qRT-PCR结果揭示了在液体培养条件下,PwDREB2D基因对干热处理的应答具有组织特异性,仅能在根状茎中被强烈诱导。在土壤栽培条件下,与对照相比时,除PwDREB2D基因以外,PwDREB2A/2AL1/2AL2基因在自然干旱-热激复合胁迫中的转录反应显著增强。与单一的自然干旱和高温处理相比时,PwDREB2AL1/2AL2基因在自然干旱-热激复合胁迫中的表达水平显著升高。由此可见,PwDREB2AL1/2AL2基因在文采报春苣苔干热复合胁迫应答中可能发挥了重要的调控作用。
