FROM May 8 to 12, 1998, the first annual computer festival was held at the Zhongguancun High-Tech Experimental Zone in Beijing’s Haidian District. Contributing to the festive atmosphere were the large and colorful ad...FROM May 8 to 12, 1998, the first annual computer festival was held at the Zhongguancun High-Tech Experimental Zone in Beijing’s Haidian District. Contributing to the festive atmosphere were the large and colorful advertisements, publicity banners, streamers, balloons and crowds buzzing about on either side of the street.展开更多
The Qiang nationqlity has a population of 198,2,52 (according to the 1991 census statistics), who mainly inhabit Maowen, Wenchuan, Lixian and Beichuan counties in Aba Tibet and Qiang Nationalities Autonomous Prefectur...The Qiang nationqlity has a population of 198,2,52 (according to the 1991 census statistics), who mainly inhabit Maowen, Wenchuan, Lixian and Beichuan counties in Aba Tibet and Qiang Nationalities Autonomous Prefecture of Sichuan Province. The Qiang is one of the oldest nationalities in China. It is said that the Qiang nationality was a main tribal race of the Xia Dynasty. The ancient Qiang nationality was an important part of the Huaxia nationality, the predecessor of the Han nationality. In a long historical process, they moved from areas in the northwest and展开更多
BACKGROUND Cardiovascular diseases are the first cause of death in the world.Ischemic heart disease is the main cause of heart failure.New approaches are continuously sou-ght to identify better therapeutic success.The...BACKGROUND Cardiovascular diseases are the first cause of death in the world.Ischemic heart disease is the main cause of heart failure.New approaches are continuously sou-ght to identify better therapeutic success.Thereby,current research has been drawn to identifying and completing the therapeutic profile of natural sources.Galium species are representatives exhibiting diuretic and antibacterial potential in living organisms and can treat burns,wounds,and skin diseases.Moreover,it was also observed that these plants manifest cardioprotective effects as well as having antihemolytic,antioxidant,antibacterial,anti-inflammatory,immunomod-ulatory,and antiproliferative potential.In ischemic heart disease,Galium verum(G.verum)extract manifested preservative properties in terms of contractility,systolic and diastolic function maintenance,and reduced damage to the heart after ischemia.In addition,G.verum extract upregulated the activity of antioxidant enzymes alleviating the production of pro-oxidants.AIM To test the ethanolic extract of G.verum on the H9C2(2-1)cell line by evaluating the in vitro biosafety profile and in ovo irritative potential.METHODS Cells were tested in vitro for viability(using the MTT test),cellular morphology,cell number,confluence,nuclear morphology(by immunofluorescence staining of cell nuclei and F-actin assay)and in ovo by the hen’s egg chorioal-lantoic membrane(CAM)test and CAM anti-irritant methods to study the irritation potential on the CAM.RESULTS The extract demonstrated a dose-dependent stimulatory activity.The viability increased to 170%for the dose of 55µg/mL and decreased to 135%at 200µg/mL.The results of cell number,confluence,and morphological analysis did not present significant changes compared with control untreated cells.The immunofluorescence assay showed insignificant apoptotic potential,and the hen’s egg CAM test revealed that the extract was in the weak to mode-rately irritating category with an irritation score of 5.3.When applying the sample to the CAM,only slight coagu-lation was observed(128 s).The anti-irritant test revealed the protective potential of the extract in the vascular plexus.CONCLUSION The ethanolic extract of G.verum manifests a stimulating effect on cardiomyocytes,enhancing cell viability,and maintaining a normal elongated shape,cell number,and confluence,without significant signs of apoptosis and with a weak irritative effect in ovo.In addition,the extract demonstrated a protective effect against hemorrhage,lysis,and coagulation of blood vessels induced by sodium dodecyl sulfate on the CAM.展开更多
文摘FROM May 8 to 12, 1998, the first annual computer festival was held at the Zhongguancun High-Tech Experimental Zone in Beijing’s Haidian District. Contributing to the festive atmosphere were the large and colorful advertisements, publicity banners, streamers, balloons and crowds buzzing about on either side of the street.
文摘The Qiang nationqlity has a population of 198,2,52 (according to the 1991 census statistics), who mainly inhabit Maowen, Wenchuan, Lixian and Beichuan counties in Aba Tibet and Qiang Nationalities Autonomous Prefecture of Sichuan Province. The Qiang is one of the oldest nationalities in China. It is said that the Qiang nationality was a main tribal race of the Xia Dynasty. The ancient Qiang nationality was an important part of the Huaxia nationality, the predecessor of the Han nationality. In a long historical process, they moved from areas in the northwest and
文摘BACKGROUND Cardiovascular diseases are the first cause of death in the world.Ischemic heart disease is the main cause of heart failure.New approaches are continuously sou-ght to identify better therapeutic success.Thereby,current research has been drawn to identifying and completing the therapeutic profile of natural sources.Galium species are representatives exhibiting diuretic and antibacterial potential in living organisms and can treat burns,wounds,and skin diseases.Moreover,it was also observed that these plants manifest cardioprotective effects as well as having antihemolytic,antioxidant,antibacterial,anti-inflammatory,immunomod-ulatory,and antiproliferative potential.In ischemic heart disease,Galium verum(G.verum)extract manifested preservative properties in terms of contractility,systolic and diastolic function maintenance,and reduced damage to the heart after ischemia.In addition,G.verum extract upregulated the activity of antioxidant enzymes alleviating the production of pro-oxidants.AIM To test the ethanolic extract of G.verum on the H9C2(2-1)cell line by evaluating the in vitro biosafety profile and in ovo irritative potential.METHODS Cells were tested in vitro for viability(using the MTT test),cellular morphology,cell number,confluence,nuclear morphology(by immunofluorescence staining of cell nuclei and F-actin assay)and in ovo by the hen’s egg chorioal-lantoic membrane(CAM)test and CAM anti-irritant methods to study the irritation potential on the CAM.RESULTS The extract demonstrated a dose-dependent stimulatory activity.The viability increased to 170%for the dose of 55µg/mL and decreased to 135%at 200µg/mL.The results of cell number,confluence,and morphological analysis did not present significant changes compared with control untreated cells.The immunofluorescence assay showed insignificant apoptotic potential,and the hen’s egg CAM test revealed that the extract was in the weak to mode-rately irritating category with an irritation score of 5.3.When applying the sample to the CAM,only slight coagu-lation was observed(128 s).The anti-irritant test revealed the protective potential of the extract in the vascular plexus.CONCLUSION The ethanolic extract of G.verum manifests a stimulating effect on cardiomyocytes,enhancing cell viability,and maintaining a normal elongated shape,cell number,and confluence,without significant signs of apoptosis and with a weak irritative effect in ovo.In addition,the extract demonstrated a protective effect against hemorrhage,lysis,and coagulation of blood vessels induced by sodium dodecyl sulfate on the CAM.
