BACKGROUND： If changes of hemodynamics in internal or external cranial artery and stenosis of atherosclerosis are found eady, patients with transient ischemic attack （TIA） may be treated at an eady phase so as to p...BACKGROUND： If changes of hemodynamics in internal or external cranial artery and stenosis of atherosclerosis are found eady, patients with transient ischemic attack （TIA） may be treated at an eady phase so as to prevent and decrease the onset of cerebral infarction. Carotid Doppler can analyze carotid canal wall, hemodynamic properties and stenosis, and changes of plaque morphology; however, transcranial Doppler （TCD） can evaluate vascular stenosis and occlusion and judge collateral circulation in cranium through detecting velocity and direction of blood flow. Can the association of them increase the diagnostic rate of TIA？ OB3ECTIVE： To evaluate the effect of the association of carotid Doppler and TCD on TIA in internal carotid artery. DESIGN： Contrast observational study SETTING： Department of Neuroelectrophysiology, Central People＇s Hospital of Huizhou PARTICIPANTS： A total of 54 patients with TIA in internal carotid artery were selected from the Department of Neurology of Huizhou Central People＇s Hospital from May 2004 to June 2005. There were 35 males and 24 females aged 46-81 years. The clinical situation was asthenia of single limb, hemiplegia, anaesthesia of single upper or lower limb, hemianesthesia, sensory disorder and aphasia. The symptoms lasted for less than 2 hours. All cases were diagnosed with CT, and those who had pathological changes of acute cerebral infarction and history of cardiac disease were excluded. Additionally, 50 healthy subjects who were regarded as control group were selected from the Department of Neurology of Huizhou Central People＇s Hospital. There were 30 males and 20 females aged 45-80 years. All subjects were consent. METHODS： HD15000 color Doppler ultrasound （Philips Company, USA） and Muliti-DopX2 TCD （DWL Company, Germany） were used to detect hemodynamics, stenosis and distribution of atherosclerosis in carotid artery and internal carotid artery. Evaluation of marker： Stenosis was calculated by the ratio between the minimal cavity and vascular sectional area at the maximal site of plaque （mild： stenosis 〈 50%; moderate and severe： stenosis t〉 50%）. With TCD, the following results were regarded as stenosis： blood velocity of average envelope 〉 120 cm/s （diagnostic criteria of vascular stenosis of basilar artery： blood velocity of average envelope 〉 80 cm/s）, increase and segmental property of blood flow, and murmu of turbulent flow and vessel. MAIN OUTCOME MEASURES： Positive rate of atherosclerosis and incidence of stenosis with carotid Doppler and TCD. RESULTS： All 54 TIA patients and 50 healthy subjects were involved in the final analysis. ① Results of carotid Doppler： Atherosclerosis and stenosis were obvious in experimental group. Positive rate of atherosclerosis was 85% （46/54） and incidence of stenosis of carotid artery was 41% （22/54）, which were higher than those in control group [52% （26/54）, 41% （22/54）, x^2 = 13.42, 10.90, P 〈 0.01]. ② Results of TCD： In experimental group, positive rate of atherosclerosis at base of skull was 89% （48/54） and incidence of stenosis of internal cranial artery was 48% （26/54）, which were higher than those in control group [62% （31/50）, 0, x^2 = 10.28, 32.00, P 〈 0.01]. Stenosis of artery was mainly involved in middle cerebral artery, initial segment and crotch of carotid artery. CONCLUSION： The association of carotid Doppler and TCD can increase clinically diagnostic rate of TIA and provide bases for apposition and intensity of TIA lesion.展开更多
BACKGROUND Anxiety is a common comorbidity in patients with Crohn’s disease(CD).Data on the imaging characteristics of brain microstructure and cerebral perfusion in CD with anxiety are limited.AIM To compare the ima...BACKGROUND Anxiety is a common comorbidity in patients with Crohn’s disease(CD).Data on the imaging characteristics of brain microstructure and cerebral perfusion in CD with anxiety are limited.AIM To compare the imaging characteristics of brain microstructure and cerebral perfusion among CD patients with or without anxiety and healthy individuals.METHODS This prospective comparative study enrolled consecutive patients with active CD and healthy individuals who visited the study hospital between January 2022 and January 2023.Anxiety was measured using the Hospital Anxiety and Depression Scale-Anxiety.The imaging characteristics of brain microstructure and cerebral perfusion were measured by diffusion kurtosis imaging and intravoxel incoherent motion.RESULTSA total of 57 participants were enrolled. Among the patients with active CD, 16 had anxiety. Compared withhealthy individuals, patients with active CD demonstrated significantly lower radial kurtosis values in the rightcerebellar region 6, lower axial kurtosis (AK) values in the right insula, left superior temporal gyrus, and rightthalamus, and higher slow and fast apparent diffusion coefficients (ADCslow and ADCfast) in the bilateral frontal lobe,bilateral temporal lobe, and bilateral insular lobe (all P < 0.05). Compared with patients with CD without anxiety,patients with CD and anxiety exhibited significantly higher ADCslow values in the left insular lobe and lower AKvalues in the right insula and right anterior cuneus (all P < 0.05).CONCLUSIONThere are variations in brain microstructure and perfusion among CD patients with/without anxiety and healthyindividuals, suggesting potential use in assessing anxiety-related changes in active CD.展开更多
BACKGROUND Chronic hepatitis C(CHC)is associated with type 2 diabetes mellitus.Although the pathogenesis remains to be elucidated,a growing evidence has suggested a role of pro-inflammatory immune response.Increased s...BACKGROUND Chronic hepatitis C(CHC)is associated with type 2 diabetes mellitus.Although the pathogenesis remains to be elucidated,a growing evidence has suggested a role of pro-inflammatory immune response.Increased serum concentrations of Interleukin 6(IL-6)have been associated with insulin resistance,type 2 diabetes mellitus as well as advanced forms of liver disease in chronic hepatitis C infection.AIM To investigate the frequency of IL-6-174G/C(rs1800795)single nucleotide polymorphism(SNP)in CHC patients and in healthy subjects of the same ethnicity.Associations between type 2 diabetes mellitus(dependent variable)and demographic,clinical,nutritional,virological and,IL-6 genotyping data were also investigated in CHC patients.METHODS Two hundred and forty-five patients with CHC and 179 healthy control subjects(blood donors)were prospectively included.Type 2 diabetes mellitus was diagnosed according to the criteria of the American Diabetes Association.Clinical,biochemical,histological and radiological methods were used for the diagnosis of the liver disease.IL-6 polymorphism was evaluated by Taqman SNP genotyping assay.The data were analysed by logistic regression models.RESULTS Type 2 diabetes mellitus,blood hypertension and liver cirrhosis were observed in 20.8%(51/245),40.0%(98/245)and 38.4%(94/245)of the patients,respectively.The frequency of the studied IL-6 SNP did not differ between the CHC patients and controls(P=0.81)and all alleles were in Hardy-Weinberg equilibrium(P=0.38).In the multivariate analysis,type 2 diabetes mellitus was inversely associated with GC and CC genotypes of IL-6-174(OR=0.42;95%CI=0.22-0.78;P=0.006)and positively associated with blood hypertension(OR=5.56;95%CI=2.79-11.09;P<0.001).CONCLUSION This study was the first to show that GC and CC genotypes of IL-6-174 SNP are associated with a decreased risk of type 2 diabetes mellitus in patients chronically infected with hepatitis C virus.The identification of potential inflammatory mediators involved in the crosstalk between hepatitis C virus and the axis pancreas-liver remains important issues that deserve further investigations.展开更多
BACKGROUND Minimally invasive or noninvasive,sensitive and accurate detection of colorectal cancer(CRC)is urgently needed in clinical practice.AIM To identify a noninvasive,sensitive and accurate circular free DNA mar...BACKGROUND Minimally invasive or noninvasive,sensitive and accurate detection of colorectal cancer(CRC)is urgently needed in clinical practice.AIM To identify a noninvasive,sensitive and accurate circular free DNA marker detected by digital polymerase chain reaction(dPCR)for the early diagnosis of clinical CRC.METHODS A total of 195 healthy control(HC)individuals and 101 CRC patients(38 in the early CRC group and 63 in the advanced CRC group)were enrolled to establish the diagnostic model.In addition,100 HC individuals and 62 patients with CRC(30 early CRC and 32 advanced CRC groups)were included separately to validate the model.CAMK1D was dPCR.Binary logistic regression analysis was used to establish a diagnostic model including CAMK1D and CEA.RESULTS To differentiate between the 195 HCs and 101 CRC patients(38 early CRC and 63 advanced CRC patients),the common biomarkers CEA and CAMK1D were used alone or in combination to evaluate their diagnostic value.The area under the curves(AUCs)of CEA and CAMK1D were 0.773(0.711,0.834)and 0.935(0.907,0.964),respectively.When CEA and CAMK1D were analyzed together,the AUC was 0.964(0.945,0.982).In differentiating between the HC and early CRC groups,the AUC was 0.978(0.960,0.995),and the sensitivity and specificity were 88.90%and 90.80%,respectively.In differentiating between the HC and advanced CRC groups,the AUC was 0.956(0.930,0.981),and the sensitivity and specificity were 81.30%and 95.90%,respectively.After building the diagnostic model containing CEA and CAMK1D,the AUC of the CEA and CAMK1D joint model was 0.906(0.858,0.954)for the validation group.In differentiating between the HC and early CRC groups,the AUC was 0.909(0.844,0.973),and the sensitivity and specificity were 93.00%and 83.30%,respectively.In differentiating between the HC and advanced CRC groups,the AUC was 0.904(0.849,0.959),and the sensitivity and specificity were 93.00%and 75.00%,respectively.CONCLUSION We built a diagnostic model including CEA and CAMK1D for differentiating between HC individuals and CRC patients.Compared with the common biomarker CEA alone,the diagnostic model exhibited significant improvement.展开更多
Objective To investigate the potential value of saccade and antisaccade parameters in early identification of Parkinson's disease(PD)and its motor subtypes.Methods A total of 111 PD patients[tremor dominant(TD)typ...Objective To investigate the potential value of saccade and antisaccade parameters in early identification of Parkinson's disease(PD)and its motor subtypes.Methods A total of 111 PD patients[tremor dominant(TD)type in 45,postural instability/gait dfficulty(PIGD)type in 54 and indeterminate type in 12]and 54 healthy controls were recruited from Department of Neurology,Guangdong Provincial People's Hospital from July 2022 to July 2023.All subjects underwent oculomotor test including visually guided saccades and volitional antisaccades by the Eyeknow-M10-B3Eye tracker.For PD patients,TD and PIGD scoreswere measured using the Movement Disorder Society Unified Parkinson's Disease Rating Scale(MDS-UPDRS)Part II and Part II.Oculomotor parameters among TD,PIGD patients and healthy controls were firstly compared.Multiple linear regression analyses were performed to assess the relationship between ocular parameters with differences and TD/PIGD score.Then receiver operating characteristic(ROC)curve analysis was made between PD patients and healthy controls,as well as between PIGD and TD subtypes.Results Compared to healthy controls,PD patients showed significantly decreased saccadic accuracy[100.0%(90.0%,100.0%)vs 100.0%(100.0%,100.0%),U=1732.500,P<0.001],prolonged latency[252.2(228.5,300.1)ms us 227.7(214.2,241.8)ms,U=1401.000,P<0.001],minimum duration[233.6(211.2,278.8)ms vs 211.0(200.0,222.5)ms,U=1534.500,P<0.001],average duration[356.6(313.8,427.8)ms vs 279.4(267.4,312.9)ms,U=881.000,P<0.001],as well as decreased peak[444.4(335.0,593.7)°/s us 526.7(412.6,696.2)°/s,U=1971.000,P=0.007]and average velocity[196.3(144.4,240.5)/s us 256.7(226.7,312.0)°/s,U=1330.000,P<0.001]in saccades.And in antisaccades,PD patients also showed prolonged latency[432.0(362.9,599.8)ms us 352.9(309.8,407.6)ms,U=1553.000,P<0.001],minimum duration[333.4(299.8,377.6)ms vs 290.1(263.9,332.9)ms,U=1608.000,P<0.001],average duration[518.2(462.7,603.5)ms vs 424.2(377.1,473.5)ms,U=1181.000,P<0.001],decreased peak[458.5(327.9,604.3)°/s vs 560.4(440.3,698.5)°/s,U=1838.500,P=0.001]and average velocity[186.6(143.1,228.1)/s vs 263.2(217.2,301.5)/s,U=1131.000,P<0.001].There was no statistically significant difference in antisaccadic accuracy[55.0%(15.0%,80.0%)vs 66.7%(39.4%,86.9%),U=2167.500,P=0.053].Compared with TD subtype,PIGD patients showed significantly decreased antisaccadic peak velocity[416.2(300.3,534.3)/s s 527.1(402.3,636.4)/s,U=-26.474,P=0.009].After adjusting for age,gender and education,antisaccadic peak velocity was negatively correlated with PIGD score in PD patients(β=-0.296,P=0.001),and no correlation with TD score was found.The ROC analysis was performed on combined saccadic and antisaccade metrics between PD patients and healthy controls,with area under the curve(AUC)as 0.918.For antisaccadic peak velocity between PIGD and TD subtypes,the AUC was 0.690.Conclusion Eye movement metrics have potential value in distinguishing PD patients from healthy controls.The antisaccadic peak velocity is related to the severity of motor symptoms in PICD patients,which is helpful for distinguishing the motor subtypes of PD patients.展开更多
Objective To study the characteristics of the airway microbiome co-occurrence network in patients with type 2 and non-type 2 asthma.Methods In a prospective study based on a cohort of asthma patients,respiratory induc...Objective To study the characteristics of the airway microbiome co-occurrence network in patients with type 2 and non-type 2 asthma.Methods In a prospective study based on a cohort of asthma patients,respiratory induced sputum samples were collected from 55 asthma patients[25 males and 30 females,with a median age of 47.7 years(age range 34.3-63.0 years)]admitted to the Department of Respiratory and Critical Care,Beijing Chaoyang Hospital,Capital Medical University and 12 healthy controls from the Physical Examination Centre of Beijing Chaoyang Hospital,Capital Medical University,from May 2021 to May 2022.According to the level of exhaled breath nitric oxide(FeNO),the asthma patients were divided into 22 cases in the high FeNO group(FeNO≥40 ppb,i.e.,type 2asthma group)and 33 cases in the low FeNO group(FeNO<40 ppb,i.e.,non-type 2 asthma group).All induced sputum samples weresubjected1 tosecond-generation macrogenomic sequencingg and bioinformatic analysessof microbial community diversity,composition1alcharacteristics,symbiotic network characteristics and metabolic function prediction.The Kruskal-Wallis rank sum test was used forbetween-group comparisonsa,nd the linear discriminant analysis(LEfSe)methodwas used to compare the differences in flora composition between groups.The R language was used for microbial network analysis.In addition,PICRUSt was used to predict the metabolic-functional characteristics ofthemicrobial communities.Results The microbial communities in the healthy control group had a lower proportion of p_Firmicutes and p_Proteobacteria than asthma patients,29%and 21%,respectively;37%and 33%in the low FeNO group and 42%and 26%in the high FeNO group.The microbial network in the low FeNO group had 64 pairs of edges forming 16 communities,and about 75%of the nodes had eigenvector centrality values between 0 and 0.05,and 25%of the nodes had eigenvector centrality values between 0.10 and 0.45.There were four layers of k-nucleosynthesis,and about 42%of the vertices were in the centre of the two layers.The microbial network of the high-FeNO group had 80 pairs of edges forming 18 clusters,and 81%of the nodes had eigenvector centrality values between 0 and 0.05,and 19%of the nodes had eigenvector centrality values between 0.10 and 0.35.The k-nucleus decomposition had eight layers,and 21%of the vertices were located in the centre's two layers.The main functional differences between the low and high FeNO groups were shown in metabolic pathways(including sugar,lipid,amino acid,and energy metabolism),drug resistance,biofilm transport,signalling,intercellular communication,and cellular repair.Conclusion Compared with non-type 2 asthmatics,type 2 asthmatics have a higher alpha diversity of respiratory microbiota,lower levels of microorganisms in the P_Proteobacteria,and a more aggregated microbial network.There is a significant difference in the predicted metabolic function of the two endotypes of asthmatics.展开更多
文摘BACKGROUND： If changes of hemodynamics in internal or external cranial artery and stenosis of atherosclerosis are found eady, patients with transient ischemic attack （TIA） may be treated at an eady phase so as to prevent and decrease the onset of cerebral infarction. Carotid Doppler can analyze carotid canal wall, hemodynamic properties and stenosis, and changes of plaque morphology; however, transcranial Doppler （TCD） can evaluate vascular stenosis and occlusion and judge collateral circulation in cranium through detecting velocity and direction of blood flow. Can the association of them increase the diagnostic rate of TIA？ OB3ECTIVE： To evaluate the effect of the association of carotid Doppler and TCD on TIA in internal carotid artery. DESIGN： Contrast observational study SETTING： Department of Neuroelectrophysiology, Central People＇s Hospital of Huizhou PARTICIPANTS： A total of 54 patients with TIA in internal carotid artery were selected from the Department of Neurology of Huizhou Central People＇s Hospital from May 2004 to June 2005. There were 35 males and 24 females aged 46-81 years. The clinical situation was asthenia of single limb, hemiplegia, anaesthesia of single upper or lower limb, hemianesthesia, sensory disorder and aphasia. The symptoms lasted for less than 2 hours. All cases were diagnosed with CT, and those who had pathological changes of acute cerebral infarction and history of cardiac disease were excluded. Additionally, 50 healthy subjects who were regarded as control group were selected from the Department of Neurology of Huizhou Central People＇s Hospital. There were 30 males and 20 females aged 45-80 years. All subjects were consent. METHODS： HD15000 color Doppler ultrasound （Philips Company, USA） and Muliti-DopX2 TCD （DWL Company, Germany） were used to detect hemodynamics, stenosis and distribution of atherosclerosis in carotid artery and internal carotid artery. Evaluation of marker： Stenosis was calculated by the ratio between the minimal cavity and vascular sectional area at the maximal site of plaque （mild： stenosis 〈 50%; moderate and severe： stenosis t〉 50%）. With TCD, the following results were regarded as stenosis： blood velocity of average envelope 〉 120 cm/s （diagnostic criteria of vascular stenosis of basilar artery： blood velocity of average envelope 〉 80 cm/s）, increase and segmental property of blood flow, and murmu of turbulent flow and vessel. MAIN OUTCOME MEASURES： Positive rate of atherosclerosis and incidence of stenosis with carotid Doppler and TCD. RESULTS： All 54 TIA patients and 50 healthy subjects were involved in the final analysis. ① Results of carotid Doppler： Atherosclerosis and stenosis were obvious in experimental group. Positive rate of atherosclerosis was 85% （46/54） and incidence of stenosis of carotid artery was 41% （22/54）, which were higher than those in control group [52% （26/54）, 41% （22/54）, x^2 = 13.42, 10.90, P 〈 0.01]. ② Results of TCD： In experimental group, positive rate of atherosclerosis at base of skull was 89% （48/54） and incidence of stenosis of internal cranial artery was 48% （26/54）, which were higher than those in control group [62% （31/50）, 0, x^2 = 10.28, 32.00, P 〈 0.01]. Stenosis of artery was mainly involved in middle cerebral artery, initial segment and crotch of carotid artery. CONCLUSION： The association of carotid Doppler and TCD can increase clinically diagnostic rate of TIA and provide bases for apposition and intensity of TIA lesion.
基金Ethics Committee of Affiliated Changzhou Second People’s Hospital of Nanjing Medical University(approval number KY039-01).
文摘BACKGROUND Anxiety is a common comorbidity in patients with Crohn’s disease(CD).Data on the imaging characteristics of brain microstructure and cerebral perfusion in CD with anxiety are limited.AIM To compare the imaging characteristics of brain microstructure and cerebral perfusion among CD patients with or without anxiety and healthy individuals.METHODS This prospective comparative study enrolled consecutive patients with active CD and healthy individuals who visited the study hospital between January 2022 and January 2023.Anxiety was measured using the Hospital Anxiety and Depression Scale-Anxiety.The imaging characteristics of brain microstructure and cerebral perfusion were measured by diffusion kurtosis imaging and intravoxel incoherent motion.RESULTSA total of 57 participants were enrolled. Among the patients with active CD, 16 had anxiety. Compared withhealthy individuals, patients with active CD demonstrated significantly lower radial kurtosis values in the rightcerebellar region 6, lower axial kurtosis (AK) values in the right insula, left superior temporal gyrus, and rightthalamus, and higher slow and fast apparent diffusion coefficients (ADCslow and ADCfast) in the bilateral frontal lobe,bilateral temporal lobe, and bilateral insular lobe (all P < 0.05). Compared with patients with CD without anxiety,patients with CD and anxiety exhibited significantly higher ADCslow values in the left insular lobe and lower AKvalues in the right insula and right anterior cuneus (all P < 0.05).CONCLUSIONThere are variations in brain microstructure and perfusion among CD patients with/without anxiety and healthyindividuals, suggesting potential use in assessing anxiety-related changes in active CD.
基金Fundationde AmparoàPesquisa do Estado de Minas Gerais,No.APQ-02320-18.
文摘BACKGROUND Chronic hepatitis C(CHC)is associated with type 2 diabetes mellitus.Although the pathogenesis remains to be elucidated,a growing evidence has suggested a role of pro-inflammatory immune response.Increased serum concentrations of Interleukin 6(IL-6)have been associated with insulin resistance,type 2 diabetes mellitus as well as advanced forms of liver disease in chronic hepatitis C infection.AIM To investigate the frequency of IL-6-174G/C(rs1800795)single nucleotide polymorphism(SNP)in CHC patients and in healthy subjects of the same ethnicity.Associations between type 2 diabetes mellitus(dependent variable)and demographic,clinical,nutritional,virological and,IL-6 genotyping data were also investigated in CHC patients.METHODS Two hundred and forty-five patients with CHC and 179 healthy control subjects(blood donors)were prospectively included.Type 2 diabetes mellitus was diagnosed according to the criteria of the American Diabetes Association.Clinical,biochemical,histological and radiological methods were used for the diagnosis of the liver disease.IL-6 polymorphism was evaluated by Taqman SNP genotyping assay.The data were analysed by logistic regression models.RESULTS Type 2 diabetes mellitus,blood hypertension and liver cirrhosis were observed in 20.8%(51/245),40.0%(98/245)and 38.4%(94/245)of the patients,respectively.The frequency of the studied IL-6 SNP did not differ between the CHC patients and controls(P=0.81)and all alleles were in Hardy-Weinberg equilibrium(P=0.38).In the multivariate analysis,type 2 diabetes mellitus was inversely associated with GC and CC genotypes of IL-6-174(OR=0.42;95%CI=0.22-0.78;P=0.006)and positively associated with blood hypertension(OR=5.56;95%CI=2.79-11.09;P<0.001).CONCLUSION This study was the first to show that GC and CC genotypes of IL-6-174 SNP are associated with a decreased risk of type 2 diabetes mellitus in patients chronically infected with hepatitis C virus.The identification of potential inflammatory mediators involved in the crosstalk between hepatitis C virus and the axis pancreas-liver remains important issues that deserve further investigations.
基金Supported by the Henan Medical Science and Technology Research Program,No.LHGJ20210045.
文摘BACKGROUND Minimally invasive or noninvasive,sensitive and accurate detection of colorectal cancer(CRC)is urgently needed in clinical practice.AIM To identify a noninvasive,sensitive and accurate circular free DNA marker detected by digital polymerase chain reaction(dPCR)for the early diagnosis of clinical CRC.METHODS A total of 195 healthy control(HC)individuals and 101 CRC patients(38 in the early CRC group and 63 in the advanced CRC group)were enrolled to establish the diagnostic model.In addition,100 HC individuals and 62 patients with CRC(30 early CRC and 32 advanced CRC groups)were included separately to validate the model.CAMK1D was dPCR.Binary logistic regression analysis was used to establish a diagnostic model including CAMK1D and CEA.RESULTS To differentiate between the 195 HCs and 101 CRC patients(38 early CRC and 63 advanced CRC patients),the common biomarkers CEA and CAMK1D were used alone or in combination to evaluate their diagnostic value.The area under the curves(AUCs)of CEA and CAMK1D were 0.773(0.711,0.834)and 0.935(0.907,0.964),respectively.When CEA and CAMK1D were analyzed together,the AUC was 0.964(0.945,0.982).In differentiating between the HC and early CRC groups,the AUC was 0.978(0.960,0.995),and the sensitivity and specificity were 88.90%and 90.80%,respectively.In differentiating between the HC and advanced CRC groups,the AUC was 0.956(0.930,0.981),and the sensitivity and specificity were 81.30%and 95.90%,respectively.After building the diagnostic model containing CEA and CAMK1D,the AUC of the CEA and CAMK1D joint model was 0.906(0.858,0.954)for the validation group.In differentiating between the HC and early CRC groups,the AUC was 0.909(0.844,0.973),and the sensitivity and specificity were 93.00%and 83.30%,respectively.In differentiating between the HC and advanced CRC groups,the AUC was 0.904(0.849,0.959),and the sensitivity and specificity were 93.00%and 75.00%,respectively.CONCLUSION We built a diagnostic model including CEA and CAMK1D for differentiating between HC individuals and CRC patients.Compared with the common biomarker CEA alone,the diagnostic model exhibited significant improvement.
文摘Objective To investigate the potential value of saccade and antisaccade parameters in early identification of Parkinson's disease(PD)and its motor subtypes.Methods A total of 111 PD patients[tremor dominant(TD)type in 45,postural instability/gait dfficulty(PIGD)type in 54 and indeterminate type in 12]and 54 healthy controls were recruited from Department of Neurology,Guangdong Provincial People's Hospital from July 2022 to July 2023.All subjects underwent oculomotor test including visually guided saccades and volitional antisaccades by the Eyeknow-M10-B3Eye tracker.For PD patients,TD and PIGD scoreswere measured using the Movement Disorder Society Unified Parkinson's Disease Rating Scale(MDS-UPDRS)Part II and Part II.Oculomotor parameters among TD,PIGD patients and healthy controls were firstly compared.Multiple linear regression analyses were performed to assess the relationship between ocular parameters with differences and TD/PIGD score.Then receiver operating characteristic(ROC)curve analysis was made between PD patients and healthy controls,as well as between PIGD and TD subtypes.Results Compared to healthy controls,PD patients showed significantly decreased saccadic accuracy[100.0%(90.0%,100.0%)vs 100.0%(100.0%,100.0%),U=1732.500,P<0.001],prolonged latency[252.2(228.5,300.1)ms us 227.7(214.2,241.8)ms,U=1401.000,P<0.001],minimum duration[233.6(211.2,278.8)ms vs 211.0(200.0,222.5)ms,U=1534.500,P<0.001],average duration[356.6(313.8,427.8)ms vs 279.4(267.4,312.9)ms,U=881.000,P<0.001],as well as decreased peak[444.4(335.0,593.7)°/s us 526.7(412.6,696.2)°/s,U=1971.000,P=0.007]and average velocity[196.3(144.4,240.5)/s us 256.7(226.7,312.0)°/s,U=1330.000,P<0.001]in saccades.And in antisaccades,PD patients also showed prolonged latency[432.0(362.9,599.8)ms us 352.9(309.8,407.6)ms,U=1553.000,P<0.001],minimum duration[333.4(299.8,377.6)ms vs 290.1(263.9,332.9)ms,U=1608.000,P<0.001],average duration[518.2(462.7,603.5)ms vs 424.2(377.1,473.5)ms,U=1181.000,P<0.001],decreased peak[458.5(327.9,604.3)°/s vs 560.4(440.3,698.5)°/s,U=1838.500,P=0.001]and average velocity[186.6(143.1,228.1)/s vs 263.2(217.2,301.5)/s,U=1131.000,P<0.001].There was no statistically significant difference in antisaccadic accuracy[55.0%(15.0%,80.0%)vs 66.7%(39.4%,86.9%),U=2167.500,P=0.053].Compared with TD subtype,PIGD patients showed significantly decreased antisaccadic peak velocity[416.2(300.3,534.3)/s s 527.1(402.3,636.4)/s,U=-26.474,P=0.009].After adjusting for age,gender and education,antisaccadic peak velocity was negatively correlated with PIGD score in PD patients(β=-0.296,P=0.001),and no correlation with TD score was found.The ROC analysis was performed on combined saccadic and antisaccade metrics between PD patients and healthy controls,with area under the curve(AUC)as 0.918.For antisaccadic peak velocity between PIGD and TD subtypes,the AUC was 0.690.Conclusion Eye movement metrics have potential value in distinguishing PD patients from healthy controls.The antisaccadic peak velocity is related to the severity of motor symptoms in PICD patients,which is helpful for distinguishing the motor subtypes of PD patients.
文摘Objective To study the characteristics of the airway microbiome co-occurrence network in patients with type 2 and non-type 2 asthma.Methods In a prospective study based on a cohort of asthma patients,respiratory induced sputum samples were collected from 55 asthma patients[25 males and 30 females,with a median age of 47.7 years(age range 34.3-63.0 years)]admitted to the Department of Respiratory and Critical Care,Beijing Chaoyang Hospital,Capital Medical University and 12 healthy controls from the Physical Examination Centre of Beijing Chaoyang Hospital,Capital Medical University,from May 2021 to May 2022.According to the level of exhaled breath nitric oxide(FeNO),the asthma patients were divided into 22 cases in the high FeNO group(FeNO≥40 ppb,i.e.,type 2asthma group)and 33 cases in the low FeNO group(FeNO<40 ppb,i.e.,non-type 2 asthma group).All induced sputum samples weresubjected1 tosecond-generation macrogenomic sequencingg and bioinformatic analysessof microbial community diversity,composition1alcharacteristics,symbiotic network characteristics and metabolic function prediction.The Kruskal-Wallis rank sum test was used forbetween-group comparisonsa,nd the linear discriminant analysis(LEfSe)methodwas used to compare the differences in flora composition between groups.The R language was used for microbial network analysis.In addition,PICRUSt was used to predict the metabolic-functional characteristics ofthemicrobial communities.Results The microbial communities in the healthy control group had a lower proportion of p_Firmicutes and p_Proteobacteria than asthma patients,29%and 21%,respectively;37%and 33%in the low FeNO group and 42%and 26%in the high FeNO group.The microbial network in the low FeNO group had 64 pairs of edges forming 16 communities,and about 75%of the nodes had eigenvector centrality values between 0 and 0.05,and 25%of the nodes had eigenvector centrality values between 0.10 and 0.45.There were four layers of k-nucleosynthesis,and about 42%of the vertices were in the centre of the two layers.The microbial network of the high-FeNO group had 80 pairs of edges forming 18 clusters,and 81%of the nodes had eigenvector centrality values between 0 and 0.05,and 19%of the nodes had eigenvector centrality values between 0.10 and 0.35.The k-nucleus decomposition had eight layers,and 21%of the vertices were located in the centre's two layers.The main functional differences between the low and high FeNO groups were shown in metabolic pathways(including sugar,lipid,amino acid,and energy metabolism),drug resistance,biofilm transport,signalling,intercellular communication,and cellular repair.Conclusion Compared with non-type 2 asthmatics,type 2 asthmatics have a higher alpha diversity of respiratory microbiota,lower levels of microorganisms in the P_Proteobacteria,and a more aggregated microbial network.There is a significant difference in the predicted metabolic function of the two endotypes of asthmatics.
