To develop a simple,validated method for identifying and quantifying 1,3-butadiene(BD) in human blood by gas chromatography-mass spectrometry(GC-MS) and head-space gas chromatography(HS-GC).BD was identified by GC-MS ...To develop a simple,validated method for identifying and quantifying 1,3-butadiene(BD) in human blood by gas chromatography-mass spectrometry(GC-MS) and head-space gas chromatography(HS-GC).BD was identified by GC-MS and HS-GC,and quantified by HS-GC.The method showed that BD had a good linearity from 50 to 500μg/mL(r>0.99).The limits of detection and quantification were 10 μg/mL and 50 μg/mL,respectively.Both the intra-day precision and inter-day precision were <6.08%,and the accuracy was 96.98%-103.81%.The method was applied to an actual case,and the concentration of BD in the case was 242 μg/mL in human blood.This simple method is found to be useful for the routine forensic analysis of acute exposure to BD.展开更多
BACKGROUND Odontogenic infection is one of the common infectious diseases in oral and maxillofacial head and neck regions.Clinically,if early odontogenic infections such as acute periapical periodontitis,alveolar absc...BACKGROUND Odontogenic infection is one of the common infectious diseases in oral and maxillofacial head and neck regions.Clinically,if early odontogenic infections such as acute periapical periodontitis,alveolar abscess,and pericoronitis of wisdom teeth are not treated timely,effectively and correctly,the infected tissue may spread up to the skull and brain,down to the thoracic cavity,abdominal cavity and other areas through the natural potential fascial space in the oral and maxillofacial head and neck.Severe multi-space infections are formed and can eventually lead to life-threatening complications(LTCs),such as intracranial infection,pleural effusion,empyema,sepsis and even death.CASE SUMMARY We report a rare case of death in a 41-year-old man with severe odontogenic multi-space infections in the oral and maxillofacial head and neck regions.One week before admission,due to pain in the right lower posterior teeth,the patient placed a cigarette butt dipped in the pesticide"Miehailin"into the"dental cavity"to relieve the pain.Within a week,the infection gradually spread bilaterally to the floor of the mouth,submandibular space,neck,chest,waist,back,temporal and other areas.The patient had difficulty breathing,swallowing and eating,and was transferred to our hospital as an emergency admission.Following admission,oral and maxillofacial surgeons immediately organized consultations with doctors in otolaryngology,thoracic surgery,general surgery,hematology,anesthesia and the intensive care unit to assist with treatment.The patient was treated with the highest level of antibiotics(vancomycin)and extensive abscess incision and drainage in the oral,maxillofacial,head and neck,chest and back regions.Unfortunately,the patient died of septic shock and multiple organ failure on the third day after admission.CONCLUSION Odontogenic infection can cause serious multi-space infections in the oral and maxillofacial head and neck regions,which can result in multiple LTCs.The management and treatment of LTCs such as multi-space infections should be multidisciplinary led by oral and maxillofacial surgeons.展开更多
Head and neck lipomas are fairly common but their presentation in areas like submandibular, submental and sublingual regions are rarely seen. Lipomas are generally asymptomatic and gradually progressive in size, causi...Head and neck lipomas are fairly common but their presentation in areas like submandibular, submental and sublingual regions are rarely seen. Lipomas are generally asymptomatic and gradually progressive in size, causing aesthetic and functional deficits. Surgical excision remains the mainstay of treatment. Radiological investigations like CECT and MRI play a critical role in diagnosis and management of these lesions. We describe a case of a giant lipoma involving the sublingual, submandibular and submental region, and its management.展开更多
为挖掘湘西黄金茶品种资源优势,区分黄金茶绿茶与其他品种烘青绿茶,本研究结合顶空固相微萃取-气相色谱-质谱(headspace-solid phase microextraction combined with gas chromatography-mass spectrometry,HS-SPMEGC-MS)联用和化学计...为挖掘湘西黄金茶品种资源优势,区分黄金茶绿茶与其他品种烘青绿茶,本研究结合顶空固相微萃取-气相色谱-质谱(headspace-solid phase microextraction combined with gas chromatography-mass spectrometry,HS-SPMEGC-MS)联用和化学计量学方法,比较了湘西州内多个产地黄金茶1号(HJC)、碧香早(BXZ)和福鼎大白(FDDB)烘青绿茶的关键挥发性化合物差异,并以此构建随机森林判别模型。感官审评结果表明,39个烘青绿茶样本的香气特征以清香、栗香为主。HS-SPME-GC-MS结果显示,共鉴定出161种挥发性化合物,HJC的总挥发性化合物、醇类和酯类化合物的含量显著高于BXZ和FDDB(P<0.05)。使用化学计量学方法分析并以变量投影重要性>1、P<0.05,且变化倍率值≥2或≤0.5为标准,共23种化合物被筛选为HJC区别于BXZ和FDDB的关键差异挥发性化合物。基于关键差异挥发性化合物构建的随机森林模型可以准确鉴别HJC和非HJC。并且香叶醇、α-松油醇等萜烯类化合物及其衍生物被认为是HJC的潜在生物标志物。研究结果可以为湘西黄金茶的质量控制和产业发展提供理论参考。展开更多
文摘To develop a simple,validated method for identifying and quantifying 1,3-butadiene(BD) in human blood by gas chromatography-mass spectrometry(GC-MS) and head-space gas chromatography(HS-GC).BD was identified by GC-MS and HS-GC,and quantified by HS-GC.The method showed that BD had a good linearity from 50 to 500μg/mL(r>0.99).The limits of detection and quantification were 10 μg/mL and 50 μg/mL,respectively.Both the intra-day precision and inter-day precision were <6.08%,and the accuracy was 96.98%-103.81%.The method was applied to an actual case,and the concentration of BD in the case was 242 μg/mL in human blood.This simple method is found to be useful for the routine forensic analysis of acute exposure to BD.
文摘BACKGROUND Odontogenic infection is one of the common infectious diseases in oral and maxillofacial head and neck regions.Clinically,if early odontogenic infections such as acute periapical periodontitis,alveolar abscess,and pericoronitis of wisdom teeth are not treated timely,effectively and correctly,the infected tissue may spread up to the skull and brain,down to the thoracic cavity,abdominal cavity and other areas through the natural potential fascial space in the oral and maxillofacial head and neck.Severe multi-space infections are formed and can eventually lead to life-threatening complications(LTCs),such as intracranial infection,pleural effusion,empyema,sepsis and even death.CASE SUMMARY We report a rare case of death in a 41-year-old man with severe odontogenic multi-space infections in the oral and maxillofacial head and neck regions.One week before admission,due to pain in the right lower posterior teeth,the patient placed a cigarette butt dipped in the pesticide"Miehailin"into the"dental cavity"to relieve the pain.Within a week,the infection gradually spread bilaterally to the floor of the mouth,submandibular space,neck,chest,waist,back,temporal and other areas.The patient had difficulty breathing,swallowing and eating,and was transferred to our hospital as an emergency admission.Following admission,oral and maxillofacial surgeons immediately organized consultations with doctors in otolaryngology,thoracic surgery,general surgery,hematology,anesthesia and the intensive care unit to assist with treatment.The patient was treated with the highest level of antibiotics(vancomycin)and extensive abscess incision and drainage in the oral,maxillofacial,head and neck,chest and back regions.Unfortunately,the patient died of septic shock and multiple organ failure on the third day after admission.CONCLUSION Odontogenic infection can cause serious multi-space infections in the oral and maxillofacial head and neck regions,which can result in multiple LTCs.The management and treatment of LTCs such as multi-space infections should be multidisciplinary led by oral and maxillofacial surgeons.
文摘Head and neck lipomas are fairly common but their presentation in areas like submandibular, submental and sublingual regions are rarely seen. Lipomas are generally asymptomatic and gradually progressive in size, causing aesthetic and functional deficits. Surgical excision remains the mainstay of treatment. Radiological investigations like CECT and MRI play a critical role in diagnosis and management of these lesions. We describe a case of a giant lipoma involving the sublingual, submandibular and submental region, and its management.
文摘为挖掘湘西黄金茶品种资源优势,区分黄金茶绿茶与其他品种烘青绿茶,本研究结合顶空固相微萃取-气相色谱-质谱(headspace-solid phase microextraction combined with gas chromatography-mass spectrometry,HS-SPMEGC-MS)联用和化学计量学方法,比较了湘西州内多个产地黄金茶1号(HJC)、碧香早(BXZ)和福鼎大白(FDDB)烘青绿茶的关键挥发性化合物差异,并以此构建随机森林判别模型。感官审评结果表明,39个烘青绿茶样本的香气特征以清香、栗香为主。HS-SPME-GC-MS结果显示,共鉴定出161种挥发性化合物,HJC的总挥发性化合物、醇类和酯类化合物的含量显著高于BXZ和FDDB(P<0.05)。使用化学计量学方法分析并以变量投影重要性>1、P<0.05,且变化倍率值≥2或≤0.5为标准,共23种化合物被筛选为HJC区别于BXZ和FDDB的关键差异挥发性化合物。基于关键差异挥发性化合物构建的随机森林模型可以准确鉴别HJC和非HJC。并且香叶醇、α-松油醇等萜烯类化合物及其衍生物被认为是HJC的潜在生物标志物。研究结果可以为湘西黄金茶的质量控制和产业发展提供理论参考。
