Cor a 14 is one of the most vital allergens in hazelnuts.However,the features of intestinal metabolites and microbiota associated with Cor a 14-induced allergy remain unclear.In this study,we established a hazelnut Co...Cor a 14 is one of the most vital allergens in hazelnuts.However,the features of intestinal metabolites and microbiota associated with Cor a 14-induced allergy remain unclear.In this study,we established a hazelnut Cor a 14-allergic BALB/c mice model,which was distinguished by the dropped temperature and enhanced allergic inflammatory factor levels in serum.Faeces were collected to detect characteristics of the intestinal metabolites by untargeted metabolomics and the gut microbiota by 16S rRNA sequencing.Theα-andβ-diversity of gut microbiota in Cor a 14-allergic mice differed from the controls with elevated relative abundance of Lactobacillus and reduced relative abundances of Odoribacter,Chloroplast,Alistipes and Lachnospiraceae_NK4A136_group.Untargeted metabolomics results revealed that 238 significantly differential metabolites(111 up-regulated,127 down-regulated)were identified,of which,L-tryptophan,histamine and N-acetylhistamine were remarkably accumulated and primarily enriched in the enhanced L-tryptophan and histidine metabolism pathways.Spearman correlation analysis suggested that L-tryptophan was positively correlated with allergic indicators and screened as the potential biomarker for Cor a 14 allergy.Collectively,our findings uncovered the characteristics of intestinal metabolites and gut microbiota during Cor a 14 anaphylaxis and provided new potential insights for diagnosis of hazelnut allergy.展开更多
Coula edulis is non-timber forest product (NTFP) used in Africa for its hazelnuts, which contain edible seeds with a demonstrated nutritional potential. However, there have been very few scientific studies of this spe...Coula edulis is non-timber forest product (NTFP) used in Africa for its hazelnuts, which contain edible seeds with a demonstrated nutritional potential. However, there have been very few scientific studies of this species in the Republic of Congo. Thus, the aim of the current study was therefore to determine the fatty acid composition of the oil extracted from Coula edulis hazelnut seeds collected at random in the Republic of Congo. The oil was extracted using the Soxhlet method and its fatty acid composition was determined by gas chromatography. The extracted oil from Coula edulis hazelnut kernels is rich in monounsaturated fatty acids (95.28%), particularly oleic acid (94.5%), which classifies it as an oleic oil and gives it interesting nutritional and therapeutic properties. On the other hand, saturated fatty acids (4.15%) and polyunsaturated fatty acids (0.35%) are not well represented. Its low poly-unsaturated fatty acid content makes it more stable when stored at room temperature.展开更多
Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processi...Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processing methods.Here,we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil(CPO),ultrasonic-assisted hexane hazelnut oil(UHO)and enzyme-assisted aqueous hazelnut oil(EAO).A total of 10 subclasses of 98 lipids were identified,including35 glycerolipids(GLs),56 glycerophospholipids(GPs)and 7 sphingolipids(SPs).The total lipid and GL content were the highest in CPO,GP content was the highest in UHO and the ceramide content in SPs was most abundant in EAO.Multivariate statistical analysis showed that the lipid profiles of hazelnut oil prepared with different processing methods varied.Twelve significantly different lipids(TAG 54:3,TAG 52:2,TAG54:4,TAG 54:2,TAG 52:3,TAG 54:5,DAG 36:2,DAG 36:4,DAG 36:3,PC 36:2,PA 36:2 and PE 36:3)were identified,and these lipids could potentially be used as biomarkers to distinguish between hazelnut oil subjected to different processing methods.Our results provide useful information for hazelnut oil applications and new insight into the effects of edible oil processing.展开更多
The study of the water status and activity of catalase in Chinese tea plants (Camellia sinensis (L.) O. Kuntze) and hazelnut (Corylus pontica C. Koch.). The indicators, which not only describe the physiological state ...The study of the water status and activity of catalase in Chinese tea plants (Camellia sinensis (L.) O. Kuntze) and hazelnut (Corylus pontica C. Koch.). The indicators, which not only describe the physiological state of plants in the stressful period, but also allow it to identify more adaptive varieties within a given area was determined. Analysis of catalase activity data in Chinese tea and hazelnut revealed the presence of similar patterns: there is a change in enzymatic activity in response to hydrothermal factors. It is established that the stable varieties Camellia sinensis (L.) O. Kuntze and Corylus pontica C. Koch. characterized by a more active catalase, which is of particular importance during droughts. The water regime parameters variance scale for Camellia sinensis was developed, which allows differentiating varieties into groups of varying degrees of stability. It is shown that all methods that are used to estimate stability of plant species to extreme environmental conditions are based on several basic principles connected with peculiarities of adaptation mechanisms. The result was a selection of diagnostic indicators proposed for assessing adaptability: the water content of the leaf tissue, water-holding capacity of leaves, and concentration of cellular juice sprouts and activity of catalase.展开更多
Although a number of bioactive peptides are capable of angiotensin I-converting enzyme (ACE) inhibitory effects, little is known regarding the mechanism of hazelnut peptides using molecular simulation. In the present ...Although a number of bioactive peptides are capable of angiotensin I-converting enzyme (ACE) inhibitory effects, little is known regarding the mechanism of hazelnut peptides using molecular simulation. In the present study, gel filtration chromatography, reverse phase-high performance liquid chromatography, and liquid chromatography-electrospray ionization-tandem mass (LC-ESI-MS/MS) were employed for purifying and identifying the ACE inhibitory peptides from hazelnut. To understand the mode of action of these peptides, the interaction between the inhibitory peptides and ACE was investigated. The results identified novel ACE inhibitory peptides Asp-Asp-Glu-Leu-Arg-Gln-Ala (DDELRQA), Asp-Asp-Glu-Leu-Arg-Ala-Ala (DDELRAA), and Asp-Gly-Glu-Leu-Arg-Glu (DGELRE). The binding free energies of DDELRQA, DDELRAA, and DGELRE for ACE were -10.2, -9.0, and -8.8 kcal/mol, respectively. This study proves the high stability of ACE inhibitory peptides derived from hazelnut against different temperature and pH of processing.展开更多
Ping′ou hybrid hazelnut is produced by cross cultivation and is widely cultivated in northern China with good development prospects.Based on a field experiment of fertilizer efficiency,the leaf spectral reflectance a...Ping′ou hybrid hazelnut is produced by cross cultivation and is widely cultivated in northern China with good development prospects.Based on a field experiment of fertilizer efficiency,the leaf spectral reflectance and leaf potassium(K)concentration were measured with different quantities of K fertilizer applied at four fruit growth stages(fruit setting stage,fruit rapid growth stage,fruit fat-change stage,and fruit near-maturity stage)of Ping′ou hybrid hazelnut in 2019.Spectral parameters that were significantly correlated with leaf K concentration were selected using Pearson correlation analysis,and spectral parameter estimation models of leaf K concentration were established by employing six different modelling methods(exponential function,power function,logarithmic function,linear function,quadratic function,and cubic function).The results indicated that at the fruit setting period,leaf K concentration was significantly correlated with Dy(spectra slope of yellow edge),Rg(reflectance of the green peak position),λo(red valley position),SDb(blue edge area),SDr/SDb(where SDr represents red edge area),and(SDr–SDb)/(SDr+SDb)(P<0.01).There were significant correlations of leaf K concentration with Dy,Rg,SDb,Rg/Ro(where Ro is the reflectance of the red valley position),and(Rg–Ro)/(Rg+Ro)at the fruit rapid growth stage(P<0.01).Further,significant correlations of leaf K concentration with Rg,Ro,RNIR/Green,and RNIR/Blue were obtained at the fruit fat-change period(P<0.01).Finally,leaf K concentration showed significant correlations with Dr,Rg,Ro,SDy(yellow edge area),and SDr at the fruit near-maturity stage(P<0.01).Through a cubic function analysis,regression estimation model of leaf K concentration with highest fitting degree(R2)values at the four fruit growth stages was established.The findings in this study demonstrated that it is feasible to estimate leaf K concentration of Ping′ou hybrid hazelnut at the various phenological stages of fruit development by establishing regression models between leaf K concentration and spectral parameters.展开更多
Turkey has been the biggest common hazelnut(Corylus avellana L.) production country in the world. The country is an unquestionable leader in this area. The average area of production is within 500 to 800 thousand ha e...Turkey has been the biggest common hazelnut(Corylus avellana L.) production country in the world. The country is an unquestionable leader in this area. The average area of production is within 500 to 800 thousand ha every year, whereas the second biggest country-Italy-has only about 125 thousand ha every year. The paper gives information about the biggest hazelnut producing countries in the world as well as the world's biggest exporters and importers. It also shows where in Turkey are the main areas of hazelnut production. It also describes the most important cultivars in its production. Moreover, it shows how the bush plant is propagated, grown, managed and fertilized and the nuts are harvested. It also points out two main insects to be the biggest problem nowadays on the Turkish plantations. Finally, it makes comments on the state's subsidization to the production, research and the newest trials with the use of mechanical harvesters on the plantations.展开更多
Hazelnut husk brown rot has been identified as a new disease in Liaoning Province in recent years.The objective of this study as to identify the pathogen.[Method]In this study,a standard sample of hazelnut husk brown ...Hazelnut husk brown rot has been identified as a new disease in Liaoning Province in recent years.The objective of this study as to identify the pathogen.[Method]In this study,a standard sample of hazelnut husk brown rot was collected from Songmudao Base in Dalian City,Liaoning Province.The pathogen was identified by the studies of the morphology,pathogenicity,and analyses of ITS and LSU sequences.The pathogen was isolated and purified,which was confirmed by Koch’s postulates.The symptoms after inoculation were the same as those collected directly from a diseased tree,which showed that it was the pathogenic fungus.The cultural characteristics and conidia and the morphology of the pathogenic fungi were similar to those of Botrytis cinerea’s.The ITS sequences and LSU sequences were compared to the associated strain sequences in GenBank,with 100%identity to Botrytis cinerea(GenBank accession number:MN589848.1)and Botrytis cinerea(GenBank accession number:KU140653.1),respectively.The infection status of the pathogen on the hazelnut husks was also observed.The studies suggested that the pathogen leading to the hazelnut husk brown rot as a new disease in Liaoning Province was Botrytis cinerea.展开更多
The aim of this study is to apply the Nutrient Analysis Critical Control Point (NACCP) process to ensure that the highest nutrient levels in food can determine a beneficial effect on the health of the consumer. The NA...The aim of this study is to apply the Nutrient Analysis Critical Control Point (NACCP) process to ensure that the highest nutrient levels in food can determine a beneficial effect on the health of the consumer. The NACCP process involves a sequence of analysis and controls that depart from raw material production to the evaluation of the effect of nutrition on health. It is articulated through the following points: 1) identification of nutrient level in the food;2) identification of critical control points (environmental, genetic data, chemical and physical data, production technology, distribution and administration);3) establishing critical limits that can impoverish and damage the nutrient;4) establishing measures to monitor;5) establishing corrective actions. We selected as biomarkers the total phenolic content (TPC) and total antioxidant capacity (TAC) of a genotyped Italian hazelnut cultivars (Corylus e avellana L.). We performed a clinical study evaluating: a) nutritional status;b) clinical-biochemical parameters;c) low density lipoprotein oxidation (LDL-ox);d) the expression level changes of oxidative stress pathway genes in the blood cell at baseline and after 40 g/die of hazelnut consumption. In this study, we found a significant lowering (p ≤ 0.005) of LDL oxidized proteins, in association with the consumption of 40 g/d of hazelnuts. Also, we found a significant variation (p ≤ 0.005) of gene expression of antioxidant and pro-oxidant genes, between the intake of dietary with and without hazelnuts. This results support the hypothesis that the NACCP process could be applied to obtain significant benefits in terms of primary prevention and for contributing to the amelioration of food management at the consumer level.展开更多
Life sciences such as medical, nutritional, and those related to agrarian biotechnologies are experiencing a constant increase. Therefore, food consumption is today related to health state. Aiming to create an innovat...Life sciences such as medical, nutritional, and those related to agrarian biotechnologies are experiencing a constant increase. Therefore, food consumption is today related to health state. Aiming to create an innovative spinneret system that defines the tight correlation between high nutritional and nutraceutical value of foods and their beneficial effects on consumer’s health state, we tested the effects related to the administration of 40 g of the Tonda Gentile Romana Italian hazelnut cultivar (Viterbo, Italy). To this end, we enrolled 24 subjects for a period of 14 weeks, based on a crossover design. Although several papers and reviews on hazelnut intake benefits are available, few studies have investigated the effects of hazelnut-enriched diet on plasma key parameters commonly used to ascertain the individual’s well-being status. The primary endpoint of the trial was to assess LDL change related to hazelnut administration, which resulted in significant beneficial effects on cholesterol parameters (LDL dropped from 133.09 mg/dl to 119.18 mg/dl). Interestingly, despite within the normal ranges, uric acid and serum creatinine mean values significantly decreased after hazelnut intake. A significant increment was also observed for ALT (from 30.9 U/L to 35.22 U/L, P = 0.01), while GGT significantly decreased from 38.04 mg/dl at T0 to 35.27 mg/dl at T1 (P = 0.001). No significant changes were also observed in azotemia, serum iron, glycemia, AST, total bilirubin, Hb, WBCs, RBCs, platelet count, and total plasma proteins. All the blood parameters analyzed appeared to be reversible after 6 weeks from the end of the treatment. Mann-Whitney U test did not show any significant differences in T0 - T1 Δ% LDL considering gender, age and baseline LDL. These findings may be of help in the development of strategies for personalized nutrition and diseases management.展开更多
Roasted and raw Turkish and Oregon hazelnuts were examined. Whole nuts, skins, and skinless nuts of both hazelnut varieties were tested for fat contents, fatty acid profiles. Hazelnut and other byproducts were extract...Roasted and raw Turkish and Oregon hazelnuts were examined. Whole nuts, skins, and skinless nuts of both hazelnut varieties were tested for fat contents, fatty acid profiles. Hazelnut and other byproducts were extracted with 50% ace- tone and examined for total phenolic contents (TPC), antioxidant activities against the peroxyl (ORAC) and DPPH radicals, and were also administered in vitro to the human colon cancer HT-29 cell line to determine antiproliferative effects. The Turkish hazelnuts contained over 65% total oil while the Oregon roasted variety contained 43.8%. The primary fatty acid in both was oleic acid (18:1n-9) comprising 76.7 g/100 g oil in the Oregon variety and 83.3 g/100 g oil in the Turkish variety. The TPC were 91.4 and 102.16 mg gallic acid equivalents/ g sample for the Turkish roasted hazelnut skin and Oregon roasted hazelnut skin respectively, at least 30-folds as high as the hazelnut without skin. Turkish roasted hazelnut skin had the highest ORAC value of 1166.27 Trolox equivalents (TE) mmol/g sample (TE mmol/g), it is 38 times as high as the Oregon roasted hazelnut no skin which as a value of 30.2 TE mmol/g sample. The range of ED50 of DPPH? is from 118.22 to 0.075 mg sample equivalents/ mL among the samples, Oregon roasted hazelnut skin and Turkish raw hazelnut no skin exhibit the weakest and strongest ability to reduce DPPH? respectively. At 6 mg/mL media Oregon roasted hazelnut skin extract significantly inhibited the growth of the HT-29 cells by 96h following 4 days of treatment, and no effect was seen from the Turkish roasted skinned hazelnut extract. The Turkish raw hazelnut had significantly higher antioxidant activities compared to the Oregon roasted variety which may be explained by chemical changes during heating or possibly the total oil to flour ratio.展开更多
Agricultural cooperatives serve as typical entities within the agricultural sector of China and encounter common representative challenges during their growth.This study employs the business model canvas theory to ana...Agricultural cooperatives serve as typical entities within the agricultural sector of China and encounter common representative challenges during their growth.This study employs the business model canvas theory to analyze the profit and business models of the Shunxiang Hazelnut Planting Cooperative while clarifying its ongoing developmental hurdles.By addressing pivotal concerns the paper puts forth branding suggestions and outlines future developmental directions.展开更多
Managing orchards requires delicate agricultural operations being typically carried out in narrow zones where the operators usually drive machineries under stress that could result in poor performance.In such conditio...Managing orchards requires delicate agricultural operations being typically carried out in narrow zones where the operators usually drive machineries under stress that could result in poor performance.In such conditions,the use of technology would help manage the machines to reduce the hazardous work and eventual damage to the plants.To safely drive a tractor,the driver needs to be aware of its surroundings,thus a stereovision system can provide helpful information.Stereo imaging has proven to be an effective three-dimensional vision system.Indeed,the range(or third coordinate)information is useful to detect the obstacle distances.Such distances,when detected during agricultural operations,could be used to assist the operator in driving the tractor at regular or variable working speeds and eventually to provide manufacturers useful indications to model the form of ROPS(roll over protection structure).This study aimed to verify the closeness of agreement between manual and stereo-image measurements,and thus to provide helpful information regarding safety and working purposes.The system used a custom low-cost dual web-camera in combination with an image analysis algorithm in order to automatically extract the information needed.Manual independent measurements were carried out using a metric tape(sensitivity 1 cm).A regular structure was used for the analysis:four rows of ten trees each one.Alternated red and blue paper markers were placed on the hazelnut trees(two per tree)of two couples of rows for enhanced visibility.For each couple of trees(one on the right,the other on the left),the four markers formed a trapezoid that was measured.The results of the analysis demonstrated that the stereo vision provided distance measurements with reasonable accuracy(error<5%)in the range of distances lower than 20 m.The resolution assessed for the developed video system is suitable for obtaining distance information in real scenes.This information could be used to assist drivers to operate agricultural machineries through narrow tree rows during work execution.Moreover,such information could be used for safeguarding decision-making and/or for controlling some tractor functions such as continuing moving,changing driving direction,changing 3-point hitch position,reducing transmission speed,halting the tractor.These functions will be necessary before tractors become fully autonomous.Finally,the measured distances,marking the narrow transitions between the tree rows,could be also used to study the ROPS form,both for working safely and for avoiding possible damage caused to the hazel trees laterally.展开更多
Brown spot formation with necrosis and sloughing off pellicle and sunken surface lesions under visibly healthy shells is one of the quality problems of Iranian hazelnuts.The symptomatic kernels were investigated for i...Brown spot formation with necrosis and sloughing off pellicle and sunken surface lesions under visibly healthy shells is one of the quality problems of Iranian hazelnuts.The symptomatic kernels were investigated for isolation of the causative bioagents.The quality attributes of infected and non-infected hazelnuts were investigated.The results showed that the total phenol content and antioxidant activity of the infected hazelnut samples were higher than non-infected samples.The infection enhanced the soluble protein content and affected the soluble protein profiles,either in the pattern or in the intensity of the protein bands.The color parameters decreased,which led to darker infected hazelnut samples.The infection also caused the alteration of the fatty acids composition of hazelnuts,which was a remarkable increment in the relative amounts of saturated fatty acids and a decrement in the unsaturated fatty acids.The optical microscopic and Scanning Electron Microscope(SEM)micrograph observation of the defective hazelnut kernel indicated the kernel parenchyma tissue had been damaged,and the microbial culture from many necrotic points revealed that this contamination due to the presence of yeast-like fungus,white to slightly creamy,round colonies with raised centers and peripheral mycelial fringes.The pathogenicity of a yeast-like fungus(isolate of NAS 201)was proved by fulfilling Koch’s postulates,and the morphological and molecular identification(internal transcribed spacer region as DNA barcoding)indicated the presence of the fungal agent Eremothecium coryli.To our knowledge,this is the first report of the occurrence of yeast spots in hazelnut in Iran.展开更多
Mapping hazelnut orchards can facilitate land planning and utilization policies,supporting the development of cooperative precision farming systems.The present work faces the detection of hazelnut crops using optical ...Mapping hazelnut orchards can facilitate land planning and utilization policies,supporting the development of cooperative precision farming systems.The present work faces the detection of hazelnut crops using optical and radar remote sensing data.A comparative study of Machine Learning techniques is presented.The system proposed utilizes multi-temporal data from the Sentinel-1 and Sentinel-2 datasets extracted over several years and processed with cloud tools.We provide a dataset of 62,982 labeled samples,with 16,561 samples belonging to the‘hazelnut’class and 46,421 samples belonging to the‘other’class,collected in 8 heterogeneous geograph-ical areas of the Viterbo province.Two different comparative tests are conducted:firstly,we use a Nested 5-Fold Cross-Validation methodology to train,optimize,and compare different Machine Learning algorithms on a single area.In a second experiment,the algorithms were trained on one area and tested on the remaining seven geo-graphical areas.The developed study demonstrates how AI analysis applied to Sentinel-1 and Sentinel-2 data is a valid technology for hazelnut mapping.From the results,it emerges that Random Forest is the classifier with the highest generalizability,achieving the best performance in the second test with an accuracy of 96%and an F1 score of 91%for the‘hazelnut’class.展开更多
基金supported by the National Key Research and Development Program of China(2019YFC1605000)。
文摘Cor a 14 is one of the most vital allergens in hazelnuts.However,the features of intestinal metabolites and microbiota associated with Cor a 14-induced allergy remain unclear.In this study,we established a hazelnut Cor a 14-allergic BALB/c mice model,which was distinguished by the dropped temperature and enhanced allergic inflammatory factor levels in serum.Faeces were collected to detect characteristics of the intestinal metabolites by untargeted metabolomics and the gut microbiota by 16S rRNA sequencing.Theα-andβ-diversity of gut microbiota in Cor a 14-allergic mice differed from the controls with elevated relative abundance of Lactobacillus and reduced relative abundances of Odoribacter,Chloroplast,Alistipes and Lachnospiraceae_NK4A136_group.Untargeted metabolomics results revealed that 238 significantly differential metabolites(111 up-regulated,127 down-regulated)were identified,of which,L-tryptophan,histamine and N-acetylhistamine were remarkably accumulated and primarily enriched in the enhanced L-tryptophan and histidine metabolism pathways.Spearman correlation analysis suggested that L-tryptophan was positively correlated with allergic indicators and screened as the potential biomarker for Cor a 14 allergy.Collectively,our findings uncovered the characteristics of intestinal metabolites and gut microbiota during Cor a 14 anaphylaxis and provided new potential insights for diagnosis of hazelnut allergy.
文摘Coula edulis is non-timber forest product (NTFP) used in Africa for its hazelnuts, which contain edible seeds with a demonstrated nutritional potential. However, there have been very few scientific studies of this species in the Republic of Congo. Thus, the aim of the current study was therefore to determine the fatty acid composition of the oil extracted from Coula edulis hazelnut seeds collected at random in the Republic of Congo. The oil was extracted using the Soxhlet method and its fatty acid composition was determined by gas chromatography. The extracted oil from Coula edulis hazelnut kernels is rich in monounsaturated fatty acids (95.28%), particularly oleic acid (94.5%), which classifies it as an oleic oil and gives it interesting nutritional and therapeutic properties. On the other hand, saturated fatty acids (4.15%) and polyunsaturated fatty acids (0.35%) are not well represented. Its low poly-unsaturated fatty acid content makes it more stable when stored at room temperature.
基金supported by Key R&D Project of Liaoning Province,under Grant Research and Demonstration of Key Technologies for Deep Processing and Comprehensive Utilization of Northeast Hazelnuts(2020JH2/10200037)Service Local Project of Liaoning Province,under Grant Demonstration and Promotion of new deep-processing technology for comprehensive utilization of Northeast Hazelnuts(LSNFW201903)horizontal subject,under Grant Demonstration and Promotion of key technologies for transformation and deep processing of wild hazelnut forest in northwestern Liaoning(H2019388)。
文摘Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processing methods.Here,we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil(CPO),ultrasonic-assisted hexane hazelnut oil(UHO)and enzyme-assisted aqueous hazelnut oil(EAO).A total of 10 subclasses of 98 lipids were identified,including35 glycerolipids(GLs),56 glycerophospholipids(GPs)and 7 sphingolipids(SPs).The total lipid and GL content were the highest in CPO,GP content was the highest in UHO and the ceramide content in SPs was most abundant in EAO.Multivariate statistical analysis showed that the lipid profiles of hazelnut oil prepared with different processing methods varied.Twelve significantly different lipids(TAG 54:3,TAG 52:2,TAG54:4,TAG 54:2,TAG 52:3,TAG 54:5,DAG 36:2,DAG 36:4,DAG 36:3,PC 36:2,PA 36:2 and PE 36:3)were identified,and these lipids could potentially be used as biomarkers to distinguish between hazelnut oil subjected to different processing methods.Our results provide useful information for hazelnut oil applications and new insight into the effects of edible oil processing.
文摘The study of the water status and activity of catalase in Chinese tea plants (Camellia sinensis (L.) O. Kuntze) and hazelnut (Corylus pontica C. Koch.). The indicators, which not only describe the physiological state of plants in the stressful period, but also allow it to identify more adaptive varieties within a given area was determined. Analysis of catalase activity data in Chinese tea and hazelnut revealed the presence of similar patterns: there is a change in enzymatic activity in response to hydrothermal factors. It is established that the stable varieties Camellia sinensis (L.) O. Kuntze and Corylus pontica C. Koch. characterized by a more active catalase, which is of particular importance during droughts. The water regime parameters variance scale for Camellia sinensis was developed, which allows differentiating varieties into groups of varying degrees of stability. It is shown that all methods that are used to estimate stability of plant species to extreme environmental conditions are based on several basic principles connected with peculiarities of adaptation mechanisms. The result was a selection of diagnostic indicators proposed for assessing adaptability: the water content of the leaf tissue, water-holding capacity of leaves, and concentration of cellular juice sprouts and activity of catalase.
文摘Although a number of bioactive peptides are capable of angiotensin I-converting enzyme (ACE) inhibitory effects, little is known regarding the mechanism of hazelnut peptides using molecular simulation. In the present study, gel filtration chromatography, reverse phase-high performance liquid chromatography, and liquid chromatography-electrospray ionization-tandem mass (LC-ESI-MS/MS) were employed for purifying and identifying the ACE inhibitory peptides from hazelnut. To understand the mode of action of these peptides, the interaction between the inhibitory peptides and ACE was investigated. The results identified novel ACE inhibitory peptides Asp-Asp-Glu-Leu-Arg-Gln-Ala (DDELRQA), Asp-Asp-Glu-Leu-Arg-Ala-Ala (DDELRAA), and Asp-Gly-Glu-Leu-Arg-Glu (DGELRE). The binding free energies of DDELRQA, DDELRAA, and DGELRE for ACE were -10.2, -9.0, and -8.8 kcal/mol, respectively. This study proves the high stability of ACE inhibitory peptides derived from hazelnut against different temperature and pH of processing.
基金the National Natural Science Foundation of China(31960324)。
文摘Ping′ou hybrid hazelnut is produced by cross cultivation and is widely cultivated in northern China with good development prospects.Based on a field experiment of fertilizer efficiency,the leaf spectral reflectance and leaf potassium(K)concentration were measured with different quantities of K fertilizer applied at four fruit growth stages(fruit setting stage,fruit rapid growth stage,fruit fat-change stage,and fruit near-maturity stage)of Ping′ou hybrid hazelnut in 2019.Spectral parameters that were significantly correlated with leaf K concentration were selected using Pearson correlation analysis,and spectral parameter estimation models of leaf K concentration were established by employing six different modelling methods(exponential function,power function,logarithmic function,linear function,quadratic function,and cubic function).The results indicated that at the fruit setting period,leaf K concentration was significantly correlated with Dy(spectra slope of yellow edge),Rg(reflectance of the green peak position),λo(red valley position),SDb(blue edge area),SDr/SDb(where SDr represents red edge area),and(SDr–SDb)/(SDr+SDb)(P<0.01).There were significant correlations of leaf K concentration with Dy,Rg,SDb,Rg/Ro(where Ro is the reflectance of the red valley position),and(Rg–Ro)/(Rg+Ro)at the fruit rapid growth stage(P<0.01).Further,significant correlations of leaf K concentration with Rg,Ro,RNIR/Green,and RNIR/Blue were obtained at the fruit fat-change period(P<0.01).Finally,leaf K concentration showed significant correlations with Dr,Rg,Ro,SDy(yellow edge area),and SDr at the fruit near-maturity stage(P<0.01).Through a cubic function analysis,regression estimation model of leaf K concentration with highest fitting degree(R2)values at the four fruit growth stages was established.The findings in this study demonstrated that it is feasible to estimate leaf K concentration of Ping′ou hybrid hazelnut at the various phenological stages of fruit development by establishing regression models between leaf K concentration and spectral parameters.
文摘Turkey has been the biggest common hazelnut(Corylus avellana L.) production country in the world. The country is an unquestionable leader in this area. The average area of production is within 500 to 800 thousand ha every year, whereas the second biggest country-Italy-has only about 125 thousand ha every year. The paper gives information about the biggest hazelnut producing countries in the world as well as the world's biggest exporters and importers. It also shows where in Turkey are the main areas of hazelnut production. It also describes the most important cultivars in its production. Moreover, it shows how the bush plant is propagated, grown, managed and fertilized and the nuts are harvested. It also points out two main insects to be the biggest problem nowadays on the Turkish plantations. Finally, it makes comments on the state's subsidization to the production, research and the newest trials with the use of mechanical harvesters on the plantations.
基金This work was financially supported by the Liaoning Provincial Natural Science Foundation of China(Grant No.2021-MS-057).
文摘Hazelnut husk brown rot has been identified as a new disease in Liaoning Province in recent years.The objective of this study as to identify the pathogen.[Method]In this study,a standard sample of hazelnut husk brown rot was collected from Songmudao Base in Dalian City,Liaoning Province.The pathogen was identified by the studies of the morphology,pathogenicity,and analyses of ITS and LSU sequences.The pathogen was isolated and purified,which was confirmed by Koch’s postulates.The symptoms after inoculation were the same as those collected directly from a diseased tree,which showed that it was the pathogenic fungus.The cultural characteristics and conidia and the morphology of the pathogenic fungi were similar to those of Botrytis cinerea’s.The ITS sequences and LSU sequences were compared to the associated strain sequences in GenBank,with 100%identity to Botrytis cinerea(GenBank accession number:MN589848.1)and Botrytis cinerea(GenBank accession number:KU140653.1),respectively.The infection status of the pathogen on the hazelnut husks was also observed.The studies suggested that the pathogen leading to the hazelnut husk brown rot as a new disease in Liaoning Province was Botrytis cinerea.
文摘The aim of this study is to apply the Nutrient Analysis Critical Control Point (NACCP) process to ensure that the highest nutrient levels in food can determine a beneficial effect on the health of the consumer. The NACCP process involves a sequence of analysis and controls that depart from raw material production to the evaluation of the effect of nutrition on health. It is articulated through the following points: 1) identification of nutrient level in the food;2) identification of critical control points (environmental, genetic data, chemical and physical data, production technology, distribution and administration);3) establishing critical limits that can impoverish and damage the nutrient;4) establishing measures to monitor;5) establishing corrective actions. We selected as biomarkers the total phenolic content (TPC) and total antioxidant capacity (TAC) of a genotyped Italian hazelnut cultivars (Corylus e avellana L.). We performed a clinical study evaluating: a) nutritional status;b) clinical-biochemical parameters;c) low density lipoprotein oxidation (LDL-ox);d) the expression level changes of oxidative stress pathway genes in the blood cell at baseline and after 40 g/die of hazelnut consumption. In this study, we found a significant lowering (p ≤ 0.005) of LDL oxidized proteins, in association with the consumption of 40 g/d of hazelnuts. Also, we found a significant variation (p ≤ 0.005) of gene expression of antioxidant and pro-oxidant genes, between the intake of dietary with and without hazelnuts. This results support the hypothesis that the NACCP process could be applied to obtain significant benefits in terms of primary prevention and for contributing to the amelioration of food management at the consumer level.
文摘Life sciences such as medical, nutritional, and those related to agrarian biotechnologies are experiencing a constant increase. Therefore, food consumption is today related to health state. Aiming to create an innovative spinneret system that defines the tight correlation between high nutritional and nutraceutical value of foods and their beneficial effects on consumer’s health state, we tested the effects related to the administration of 40 g of the Tonda Gentile Romana Italian hazelnut cultivar (Viterbo, Italy). To this end, we enrolled 24 subjects for a period of 14 weeks, based on a crossover design. Although several papers and reviews on hazelnut intake benefits are available, few studies have investigated the effects of hazelnut-enriched diet on plasma key parameters commonly used to ascertain the individual’s well-being status. The primary endpoint of the trial was to assess LDL change related to hazelnut administration, which resulted in significant beneficial effects on cholesterol parameters (LDL dropped from 133.09 mg/dl to 119.18 mg/dl). Interestingly, despite within the normal ranges, uric acid and serum creatinine mean values significantly decreased after hazelnut intake. A significant increment was also observed for ALT (from 30.9 U/L to 35.22 U/L, P = 0.01), while GGT significantly decreased from 38.04 mg/dl at T0 to 35.27 mg/dl at T1 (P = 0.001). No significant changes were also observed in azotemia, serum iron, glycemia, AST, total bilirubin, Hb, WBCs, RBCs, platelet count, and total plasma proteins. All the blood parameters analyzed appeared to be reversible after 6 weeks from the end of the treatment. Mann-Whitney U test did not show any significant differences in T0 - T1 Δ% LDL considering gender, age and baseline LDL. These findings may be of help in the development of strategies for personalized nutrition and diseases management.
文摘Roasted and raw Turkish and Oregon hazelnuts were examined. Whole nuts, skins, and skinless nuts of both hazelnut varieties were tested for fat contents, fatty acid profiles. Hazelnut and other byproducts were extracted with 50% ace- tone and examined for total phenolic contents (TPC), antioxidant activities against the peroxyl (ORAC) and DPPH radicals, and were also administered in vitro to the human colon cancer HT-29 cell line to determine antiproliferative effects. The Turkish hazelnuts contained over 65% total oil while the Oregon roasted variety contained 43.8%. The primary fatty acid in both was oleic acid (18:1n-9) comprising 76.7 g/100 g oil in the Oregon variety and 83.3 g/100 g oil in the Turkish variety. The TPC were 91.4 and 102.16 mg gallic acid equivalents/ g sample for the Turkish roasted hazelnut skin and Oregon roasted hazelnut skin respectively, at least 30-folds as high as the hazelnut without skin. Turkish roasted hazelnut skin had the highest ORAC value of 1166.27 Trolox equivalents (TE) mmol/g sample (TE mmol/g), it is 38 times as high as the Oregon roasted hazelnut no skin which as a value of 30.2 TE mmol/g sample. The range of ED50 of DPPH? is from 118.22 to 0.075 mg sample equivalents/ mL among the samples, Oregon roasted hazelnut skin and Turkish raw hazelnut no skin exhibit the weakest and strongest ability to reduce DPPH? respectively. At 6 mg/mL media Oregon roasted hazelnut skin extract significantly inhibited the growth of the HT-29 cells by 96h following 4 days of treatment, and no effect was seen from the Turkish roasted skinned hazelnut extract. The Turkish raw hazelnut had significantly higher antioxidant activities compared to the Oregon roasted variety which may be explained by chemical changes during heating or possibly the total oil to flour ratio.
基金2019 Jilin Province College Students'Science and Technology Innovation"Research on the Construction and Promotion Path of Xilangzhen Brand under the Background of Rural Revitalization"(Project number:S2021111439056)。
文摘Agricultural cooperatives serve as typical entities within the agricultural sector of China and encounter common representative challenges during their growth.This study employs the business model canvas theory to analyze the profit and business models of the Shunxiang Hazelnut Planting Cooperative while clarifying its ongoing developmental hurdles.By addressing pivotal concerns the paper puts forth branding suggestions and outlines future developmental directions.
基金Part of this study was funded by the project“ALForLab”(PON03PE_00024_1)co-funded by the Italian National Operational Programme for Research and Competitiveness(PON R&C)2007-2013through the European Regional Development Fund(ERDF)and the national resource Revolving Fund-Cohesion Action Plan(CAP)MIUR and project AGROENER(D.D.n.26329)funded by Italian Ministry of Agriculture,Food and Forestry Policies(MiPAAF).
文摘Managing orchards requires delicate agricultural operations being typically carried out in narrow zones where the operators usually drive machineries under stress that could result in poor performance.In such conditions,the use of technology would help manage the machines to reduce the hazardous work and eventual damage to the plants.To safely drive a tractor,the driver needs to be aware of its surroundings,thus a stereovision system can provide helpful information.Stereo imaging has proven to be an effective three-dimensional vision system.Indeed,the range(or third coordinate)information is useful to detect the obstacle distances.Such distances,when detected during agricultural operations,could be used to assist the operator in driving the tractor at regular or variable working speeds and eventually to provide manufacturers useful indications to model the form of ROPS(roll over protection structure).This study aimed to verify the closeness of agreement between manual and stereo-image measurements,and thus to provide helpful information regarding safety and working purposes.The system used a custom low-cost dual web-camera in combination with an image analysis algorithm in order to automatically extract the information needed.Manual independent measurements were carried out using a metric tape(sensitivity 1 cm).A regular structure was used for the analysis:four rows of ten trees each one.Alternated red and blue paper markers were placed on the hazelnut trees(two per tree)of two couples of rows for enhanced visibility.For each couple of trees(one on the right,the other on the left),the four markers formed a trapezoid that was measured.The results of the analysis demonstrated that the stereo vision provided distance measurements with reasonable accuracy(error<5%)in the range of distances lower than 20 m.The resolution assessed for the developed video system is suitable for obtaining distance information in real scenes.This information could be used to assist drivers to operate agricultural machineries through narrow tree rows during work execution.Moreover,such information could be used for safeguarding decision-making and/or for controlling some tractor functions such as continuing moving,changing driving direction,changing 3-point hitch position,reducing transmission speed,halting the tractor.These functions will be necessary before tractors become fully autonomous.Finally,the measured distances,marking the narrow transitions between the tree rows,could be also used to study the ROPS form,both for working safely and for avoiding possible damage caused to the hazel trees laterally.
基金supported by the Center for International Scientific Studies and Collaboration(CISSC),Ministry of Science,Research and Technology,Iran.
文摘Brown spot formation with necrosis and sloughing off pellicle and sunken surface lesions under visibly healthy shells is one of the quality problems of Iranian hazelnuts.The symptomatic kernels were investigated for isolation of the causative bioagents.The quality attributes of infected and non-infected hazelnuts were investigated.The results showed that the total phenol content and antioxidant activity of the infected hazelnut samples were higher than non-infected samples.The infection enhanced the soluble protein content and affected the soluble protein profiles,either in the pattern or in the intensity of the protein bands.The color parameters decreased,which led to darker infected hazelnut samples.The infection also caused the alteration of the fatty acids composition of hazelnuts,which was a remarkable increment in the relative amounts of saturated fatty acids and a decrement in the unsaturated fatty acids.The optical microscopic and Scanning Electron Microscope(SEM)micrograph observation of the defective hazelnut kernel indicated the kernel parenchyma tissue had been damaged,and the microbial culture from many necrotic points revealed that this contamination due to the presence of yeast-like fungus,white to slightly creamy,round colonies with raised centers and peripheral mycelial fringes.The pathogenicity of a yeast-like fungus(isolate of NAS 201)was proved by fulfilling Koch’s postulates,and the morphological and molecular identification(internal transcribed spacer region as DNA barcoding)indicated the presence of the fungal agent Eremothecium coryli.To our knowledge,this is the first report of the occurrence of yeast spots in hazelnut in Iran.
文摘Mapping hazelnut orchards can facilitate land planning and utilization policies,supporting the development of cooperative precision farming systems.The present work faces the detection of hazelnut crops using optical and radar remote sensing data.A comparative study of Machine Learning techniques is presented.The system proposed utilizes multi-temporal data from the Sentinel-1 and Sentinel-2 datasets extracted over several years and processed with cloud tools.We provide a dataset of 62,982 labeled samples,with 16,561 samples belonging to the‘hazelnut’class and 46,421 samples belonging to the‘other’class,collected in 8 heterogeneous geograph-ical areas of the Viterbo province.Two different comparative tests are conducted:firstly,we use a Nested 5-Fold Cross-Validation methodology to train,optimize,and compare different Machine Learning algorithms on a single area.In a second experiment,the algorithms were trained on one area and tested on the remaining seven geo-graphical areas.The developed study demonstrates how AI analysis applied to Sentinel-1 and Sentinel-2 data is a valid technology for hazelnut mapping.From the results,it emerges that Random Forest is the classifier with the highest generalizability,achieving the best performance in the second test with an accuracy of 96%and an F1 score of 91%for the‘hazelnut’class.
