Birdsfoot trefoil (Lotus corniculatus L.) is high in nutritional value and adapted to slightly acidic, droughty, infertile, or wet soils. However, the impermeable seed coat restricts water imbibition and results in ...Birdsfoot trefoil (Lotus corniculatus L.) is high in nutritional value and adapted to slightly acidic, droughty, infertile, or wet soils. However, the impermeable seed coat restricts water imbibition and results in poor seed germination and subse- quent stand establishment. The aim of this study was to identify the dormancy mechanism of Birdsfoot trefoil seeds, and investigate the suitable pretreatment to overcome the poor seed germination. Germination behavior was characterized by using the final germination percentage and the mean germination time. The results indicated that coat thickness of dormant seeds was significant larger. The average hard seed rate was up to 92.3%. It was found that mechanical scarification, chemi- cal scarification with concentrated sulfuric acid (98% H_2SO_4), and gibberellic acid soaking following freezing below zero temperature were effective in reducing hard- seededness and lead to increases of seed germination.展开更多
[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. (Leguminosae) and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean see...[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. (Leguminosae) and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of (50.08-58.26 g); among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.展开更多
基金Supported by National Natural Science Foundation of China(No.31601997)~~
文摘Birdsfoot trefoil (Lotus corniculatus L.) is high in nutritional value and adapted to slightly acidic, droughty, infertile, or wet soils. However, the impermeable seed coat restricts water imbibition and results in poor seed germination and subse- quent stand establishment. The aim of this study was to identify the dormancy mechanism of Birdsfoot trefoil seeds, and investigate the suitable pretreatment to overcome the poor seed germination. Germination behavior was characterized by using the final germination percentage and the mean germination time. The results indicated that coat thickness of dormant seeds was significant larger. The average hard seed rate was up to 92.3%. It was found that mechanical scarification, chemi- cal scarification with concentrated sulfuric acid (98% H_2SO_4), and gibberellic acid soaking following freezing below zero temperature were effective in reducing hard- seededness and lead to increases of seed germination.
基金Supported by the"Twelfth Five-Year"Key Project for Crop Breeding of Sichuan Province(YZGG4-6)the Special Support Program for the Scientific Research Personnel of Sichuan Agricultural University~~
文摘[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. (Leguminosae) and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of (50.08-58.26 g); among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.