Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle H...Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle Harbor over a year,with monthly and quarterly sampling.Both species richness and bacterial numbers exhibited seasonal variations.Specifically,the abundance of attached bacteria and bacterioplankton peaked in June and July,corresponding to higher water temperatures in summer and autumn,while lower abundances were noted in January and December during cooler periods.Throughout the year,the species richness of attached bacteria consistently exceeded that of planktonic bacteria in both shallow and deep waters.Pseudoalteromonas emerged as the most prevalent genus among both planktonic and attached bacteria in surface and bottom seawater samples.Furthermore,the magnitude of changes in species richness and abundance for attached bacteria(0.66×10^(5)-15.85×10^(5)CFU/cm^(2))was greater than that observed for planktonic bacteria(0.58×10^(8)-5.33×10^(8)CFU/L).We propose that the attached bacterial populations,situated in limited microenvironments within the larger seawater ecosystem,exhibit heightened sensitivity to environmental fluctuations,resulting in more rapid shifts in population dynamics and lower ecological stability.The theoretical implications and potential applications of these findings warrant further investigation.展开更多
The distribution characteristics of heavy metals such as Cu, Zn, Pb, Cd, Hg. As in seawater, superficail sediments and several marine organisms of Dongzhai Harbor in Hainan were analyzed. Index methods of the single f...The distribution characteristics of heavy metals such as Cu, Zn, Pb, Cd, Hg. As in seawater, superficail sediments and several marine organisms of Dongzhai Harbor in Hainan were analyzed. Index methods of the single factor and geo-accumulation were used to assess the pollution degree of heavy metals. The results showed that the heavy metal levels in the seawater of Dongzhai Harbor accorded with the first category of the seawater except Pb, and were much lower than the fishery water quality standard; the concentration of the heavy metal in surface sediments did not exceed the national benchmark of the first-class of marine sediment quality basically, and the superficial sediments were essentially uncontaminated; the heavy metal content in wetland sediments was slightly higher than those in other typical mangrove wetland island of Hainan and marine sediments in Dongzhai Haibor, which indicated this area might has been affected by land-based pollution influence, and this should cause the attention of related departments; the heavy metal concentrations in marine organisms were up to the first category. All were not beyond the standard of non-environmental pollution aquatic products.展开更多
Temporal and spatial variations of concentrations of heavy metals including mercury （Hg）, zinc （Zn）, lead （Pb）, arsenic （As）, copper （Cu）, and cadmium （Cd） in the sediments of the Yangshan Deepwater Harbor...Temporal and spatial variations of concentrations of heavy metals including mercury （Hg）, zinc （Zn）, lead （Pb）, arsenic （As）, copper （Cu）, and cadmium （Cd） in the sediments of the Yangshan Deepwater Harbor were determined based on 6 cruises in autumn and winter, respectively, from 2010 to 2013. The results demonstrated that the overall concentrations of heavy metals were low and distributed in uniform patterns. The concentrations of Hg, Zn, Pb, and Cd in autumn were significantly higher than those in winter with small fluctuations for As and Cu in terms of seasonal variations. Results of factor analysis showed that Pb, Cd, and Zn were derived from inland industrial and shipping discharges as well as the degradation of organic pollutants in marine environment. While agricultural pollutions, cargo shifting and construction debris from reclamation projects contributed to the sources of Cu, As, and Hg. Ecological risk assessment by Mean Sediment Quality Guideline Quotient （SQG-Q） revealed that the degree for eco-risk of the sediments was low-and-moderate in autumn, higher than that in winter. Hg and Cu were the dominant eco-risk factors. The results of Index of geoaccumulation （Igeo） showed that the whole sites of the sea area were barely influenced by Hg, As, Zn, and Pb, and the extents of Cd and Cu contaminations were in low grade. Contamination degree of all the six heavy metals could be ranked as the following： Cd〉Pb〉Zn〉Hg〉As. According to the results of integrated score of factor analysis, the contamination degree for heavy metals in sediments of the Yangshan Deepwater Harbor was low, despite sites No.5, No.4 and No.3, which were heavily contaminated compared with others.展开更多
Based on the characteristics of waves, tidal currents, sediment and seabed evolution in the Caofeidian sea area in the Bohai Bay, a 2D sediment mathematical model of waves and tidal currents is employed to study the d...Based on the characteristics of waves, tidal currents, sediment and seabed evolution in the Caofeidian sea area in the Bohai Bay, a 2D sediment mathematical model of waves and tidal currents is employed to study the development schemes of the harbor. Verification of spring and neap tidal currents and sediment in the winter and summer of 2006 shows that the calculated values of tidal stages as well as flow velocities, flow directions and sediment concentration of 15 synchronous vertical lines are in good agreement with the measured data. Also, deposition and erosion of the sea area in front of Caofeidian ore terminal induced by suspended load under tidal currents and waves are verified; it shows that the calculated values of depth of deposition and erosion as well as their distribution are close to the measured data. Furthermore, effects of reclamation scheme of island in front of the land behind Caofeidian harbor on the hydrodynamic environment are studied, including changes of flow velocities in the deep channels at the south side of Caofeidian foreland and Laolonggou and in various harbor basins, as well as changes of deposition and erosion of seabed induced by the project.展开更多
The general features of oscillations within a rectangular harbor of exponential bottom are investigated analytically. Based on the linear shallow water approximation, analytical solutions for longitudinal oscillations...The general features of oscillations within a rectangular harbor of exponential bottom are investigated analytically. Based on the linear shallow water approximation, analytical solutions for longitudinal oscillations induced by the incident perpendicular wave are obtained by the method of matched asymptotics. The analytic results show that the resonant frequencies are shifted to larger values as the water depth increases and the oscillation amplitudes are enhanced due to the shoaling effect. Owing to the refraction effect, there could be several transverse oscillation modes existing in when the width of the harbor is on the order of the oscillation wavelength. These transverse oscillations are similar to standing edge waves, and there are m node lines in the longshore direction and n node lines running in the offshore direction corresponding to mode（n, m）. Furthermore, the transverse eigen frequency is not only related to the width of the harbor, but also to the boundary condition at the backwall and the bottom shape.展开更多
The long-term temporal and spatial dynamics of marine coastal water quality in Tolo Harbor, Hong Kong were explored. The Harbor is divided into three zones represented as Harbor, Buffer, and Channel Subzones. The time...The long-term temporal and spatial dynamics of marine coastal water quality in Tolo Harbor, Hong Kong were explored. The Harbor is divided into three zones represented as Harbor, Buffer, and Channel Subzones. The time range for the study covers the period from the 1970s to the 1990s. The selected indicators for the comprehensive assessment of water quality consist of physical, chemical and biological aspects, including suspended solids(SS), Secchi disk depth(SD), 5-day biochemical oxygen demand(BOD\-5), total nitrogen(TN), total phosphorus(TP), faecal coliform, chlorophyll-a(Chl-a), and the number of red tide occurrences. The results indicated the presence of obvious temporal and spatial trends with regard to changes in water quality. Spatially, water quality in the Channel Subzone is the best, while that in the Harbor Subzone is the worst. On a temporal basis, the average trend from bad to good was 1980s>1990s>1970s as indicated by most of the selected water quality indicators. Water quality during the late 1980s reached its worst level with the lowest SD, the highest BOD\-5, TN, TP, Chl-a concentrations, and the number of red tide occurrences. These long-term temporal-spatial water quality trends were also found in other studies of the Tolo Harbor. The large quantity of pollutants produced as a result of increasing population, industrial and commercial actives, and urbanization and industrialization trends in both Shatin and Tai Po seem to be primarily responsible for the changes in marine coastal water quality.展开更多
A composite numerical model is presented for computing the wave field in a harbor. The mild slope equation is discretized by a finite element method in the domain concerned. Out of the computational domain, the water ...A composite numerical model is presented for computing the wave field in a harbor. The mild slope equation is discretized by a finite element method in the domain concerned. Out of the computational domain, the water depth is assumed to be constant. The boundary element method is applied to the outer boundary for dealing with the infinite boundary condition. Because the model satisfies strictly the infinite boundary condition, more accurate results can be obtained. The model is firstly applied to compute the wave diffraction in a narrow rectangular bay and the wave diffraction from a porous cylinder. The numerical results are compared with the analytical solution, experimental data and other numerical results. Good agreements are obtained. Then the model is applied to computing the wave diffraction in a square harbor with varying water depth. The effects of the water depth in the harbor and the incoming wave direction on the wave height distribution are discussed.展开更多
A membrane bioreactor （MBR） was established for treating harbor oily wastewater. It showed good removal performance for chemical oxygen demand （COD）, oil content, suspended solids （SS）, and other pollutants. How...A membrane bioreactor （MBR） was established for treating harbor oily wastewater. It showed good removal performance for chemical oxygen demand （COD）, oil content, suspended solids （SS）, and other pollutants. However, serious membrane fouling occurred. It was recognized that the extracellular polymeric substances （EPS） accumulated on the membrane surface, especially the proteins, were of great importance for the transmembrane pressure （TMP） increment and membrane fouling. The MBR was optimized via improving aeration rate and reducing the ratio of Ar/Ad （At and Ad are the cross-sectional areas of the riser and the downcomer of the MBR）. The increasing rate of TMP was slowed, indicating that the optimization strategy could effectively mitigate membrane fouling. Microbial community evolution was monitored and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）, cloning, and sequencing of 16S ribosomal ribonucleic acid （rRNA） fragments. Results revealed that low community shift occurred during the whole operational period. Geobacter sp. and Rhodocyclales sp., which have also been identified by other studies in a petroleum refinery wastewater MBR or an infiltration basin receiving highway runoff, dominated in the MBR system throughout. Comamonas sp. was thought to accommodate the lower aeration rate in this study, while Rhodocyclales sp. preferred the higher aeration rate. In addition, during the operational time under high organic loading rate, a few species were present in abundance, and may have been responsible for the good removal performance at this time.展开更多
The influence of in-port ship emissions on gases and PM10 concentrations has been estimated in the port city of Calais, northern France, one of the busiest harbor in Europe, with numerous rotations of ferries or roll-...The influence of in-port ship emissions on gases and PM10 concentrations has been estimated in the port city of Calais, northern France, one of the busiest harbor in Europe, with numerous rotations of ferries or roll-on/roll-off cargo in average per day. NOx, SO2, O3 and PM10 concentrations were continuously measured over a three-month period, as well as real-time particle size distribution. A rural site located at Cape Gris-Nez, 20 km from Calais, was considered to deduce intrinsic contribution of ship emissions at the harbor city. The average concentrations of the studied species as well as the pattern of the conditional bivariate probability function at the two sites evidenced that in-port shipping, especially during the maneuvering operations, has an important influence on the NOx and SO2 concentrations. The impact of shipping in the harbor of Calais on average concentrations was estimated to 5l% for SO2, 35% for NO, 15% for NO2 and 2% for PM10 in the studied period. Concentration peaks of SO2 and NOx associated with an 03 depletion appeared synchronized with departures and arrivals of ferries. For winds blowing from the harbor, when compared to the background level, the number of particles appeared 10 times higher, with the highest differences in the 30-67 nm and the 109-167 nm size ranges. The average impact of in-port ships on PM10 concentrations was estimated to ＋28.9 μg/m^3 and concerned mainly the PM1 size fraction （40%）. Punctually, PM10 can potentially reach a concentration value close to 100μg/m^3.展开更多
Resulting from the rising levels of atmospheric carbon, ocean acidification has become a global problem. It has significant impacts on the development, survival, growth and physiology of marine organisms. Therefore, a...Resulting from the rising levels of atmospheric carbon, ocean acidification has become a global problem. It has significant impacts on the development, survival, growth and physiology of marine organisms. Therefore, a high- precision sensor is urgently needed to measure the pH of sea-water. Iridium wire with a diameter of 0.25 mm is used as the substrate, and an Ir/Ir（OH）x pH electrode is prepared by a one-step electrochemical method in a LiOH solution at the room temperature. A scanning electron microscope （SEM） observation reveals that it is coated with nanoscale particles. In laboratory tests, the electrode exhibits a very promising pH response, with an ideal Nernst slope （56.14-59.52）, fast response, good stability and long fife-span in tested pH buffer solutions. For a sea trial, four pH electrodes and one Ag/AgC1 reference electrode are integrated with a self-made chemical sensor, and a profile detection of nearly 70 m is implemented near Newport Harbor, California on August 3, 2015. The results reflect that the pH value measured by the sensor is very close to the data given by Sea-Bird 911 plus CTD, with a difference value ranging from 0.000 075 to 0.064 719. And the sensor shows a better data matching degree in 0-40 m water depth. In addition, the high precision and accuracy of the sensor make it possible to use in the ocean observation field.展开更多
In this paper,the open-sourced computational fluid dynamics software,OpenFOAM~?,is used to study the fluctuation phenomenon of the water body inside a horizontally one-dimensional enclosed harbor basin with constant w...In this paper,the open-sourced computational fluid dynamics software,OpenFOAM~?,is used to study the fluctuation phenomenon of the water body inside a horizontally one-dimensional enclosed harbor basin with constant water depth triggered by falling wedges with various horizontal falling positions,initial falling velocities and masses.Based on both Fourier transfo rm analysis and wavelet spectrum analysis for the time series of the free surface elevations inside the harbor basin,it is found for the first time that the wedge falling inside the harbor can directly trigger harbor resonance.The influences of the three factors(including the horizontal falling position,the initial falling velocity,and the mass)on the response amplitudes of the lowest three resonant modes are also investigated.The results show that when the wedge falls on one of the nodal points of a resonant mode,the mode would be remarkably suppressed.Conversely,when the wedge falls on one of the anti-nodal points of a resonant mode,the mode would be evidently triggered.The initial falling velocity of the wedge mainly has a remarkable effect on the response amplitude of the most significant mode,and the latter shows a gradual increase trend with the increase of the former.While for the other two less significant modes,their response amplitudes fluctuate around certain constant values as the initial falling velocity rises.In general,the response amplitudes of all the lowest three modes are shown to gradually increase with the mass of the wedge.展开更多
By analysis of published papers on the Yangtze estuary and hydrological and sediments data in Yangshan Harbor area, many similarities are found between Yangshan Harbor area and the Yangtze estuary. These similarities ...By analysis of published papers on the Yangtze estuary and hydrological and sediments data in Yangshan Harbor area, many similarities are found between Yangshan Harbor area and the Yangtze estuary. These similarities include the phenomenon of stagnating flow areas, the distributive characteristics of the highest suspended sediment concentration areas, superficial sediments and shoal bars. The stagnating flow area is the major similarity which causes other similarities. These similarities indicate that: 1) Turbidity Maximum and mouth bars in estuaries are mainly caused by the hydraulic balance of stagnating flow areas of estuaries; 2) The stagnating sand area of sands caused by stagnating flow area often locates on the narrower side of the stagnating flow area; 3) The location (or shape) of fine sediments area caused by stagnating flow area reflects the location (or shape) of the stagnating flow area. Both Yangshan Harbor area and the Yangtze estuary are the important developmental areas in the future (man-made similarity). In-depth studies on these similarities between Yangshan Harbor area and the Yangtze estuary will have momentous theoretical and practical significance.展开更多
Influences of topographic variations of the offshore fringing reef on the harbor oscillations excited by incident Nwaves with different amplitudes and waveform types are studied for the first time.Both the propagation...Influences of topographic variations of the offshore fringing reef on the harbor oscillations excited by incident Nwaves with different amplitudes and waveform types are studied for the first time.Both the propagation of the Nwaves over the reef and the subsequently-induced harbor oscillations are simulated by a Boussinesq-type numerical model,FUNWAVE-TVD.The present study concentrates on revealing the influences of the plane reef-face slope,the reef-face profile shape and the lagoon width on the maximum runup,the wave energy distribution and the total wave energy within the harbor.It shows that both the wave energy distribution uniformity and the total wave energy gradually increase with decreasing reef-face slope.The profile shape of the reef face suffering leading-elevation Nwaves(LEN waves)has a negligible impact on the wave energy distribution uniformity,while for leading-depression N-waves(LDN waves),the latter gradually decreases with the mean water depth over the reef face.The total wave energy always first increases and then decreases with the mean water depth over the reef face.In general,the total wave energy first sharply decreases and then slightly increases with the lagoon width,regardless of the reef-face width and the incident waveform type.The maximum runup subjected to the LEN waves decreases monotonously with the lagoon width.However,for the LDN waves,its changing trend with the lagoon width relies on the incident wave amplitude.展开更多
Dear Editor,Structural aberrations of the Y chromosome,including deletions,ring chromosomes,Y-autosomal or Y-X translocations,isochromosomes,and dicentrics,occur in approximately 0.2%of live births;1 among these,parti...Dear Editor,Structural aberrations of the Y chromosome,including deletions,ring chromosomes,Y-autosomal or Y-X translocations,isochromosomes,and dicentrics,occur in approximately 0.2%of live births;1 among these,partial deletion in the azoospermia factor(AZF)region of the Y chromosome is a well-characterized cause of nonobstructive severe oligozoospermia and azoospermia.2 Meanwhile,a dicentric Y chromosome,abbreviated as dic(Y),was observed to be exclusively present in males with azoospermia,suggesting more severe negative consequences of the AZF deletion for spermatogenesis.展开更多
Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent o...Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent overexpression of foreign genes,without side effects.However,relatively few safe harbor loci are available in pigs,a fact which has impeded the development of multi-transgenic pig research.We report a strategy for efficient transgene knock-in in the endogenous collagen type I alpha 1 chain(COL1A1)gene using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system.After the knock-in of a 2A peptide-green fluorescence protein(2A-GFP)transgene in the last codon of COL1A1 in multiple porcine cells,including porcine kidney epithelial(PK15),porcine embryonic fibroblast(PEF)and porcine intestinal epithelial(IPI-2I)cells,quantitative PCR(qPCR),Western blotting,RNA-seq and CCK8 assay were performed to assess the safety of COL1A1 locus.The qPCR results showed that the GFP knock-in had no effect(P=0.29,P=0.66 and P=0.20 for PK15,PEF and IPI-2I cells,respectively)on the mRNA expression of COL1A1 gene.Similarly,no significant differences(P=0.64,P=0.48 and P=0.80 for PK15,PEF and IPI-2I cells,respectively)were found between the GFP knock-in and wild type cells by Western blotting.RNA-seq results revealed that the transcriptome of GFP knock-in PEF cells had a significant positive correlation(P<2.2e–16)with that of the wild type cells,indicating that the GFP knock-in did not alter the global expression of endogenous genes.Furthermore,the CCK8 assay showed that the GFP knock-in events had no adverse effects(P_(24)h=0.31,P_(48)h=0.96,P_(72)h=0.24,P_(96)h=0.17,and P_(120)h=0.38)on cell proliferation of PK15 cells.These results indicate that the COL1A1 locus can be used as a safe harbor for foreign genes knock-in into the pig genome and can be broadly applied to farm animal breeding and biomedical model establishment.展开更多
The motion of the moored ship in the harbor is a classical hydrodynamics problem that still faces many challenges in naval operations,such as cargo transfer and ship pairings between a big transport ship and some smal...The motion of the moored ship in the harbor is a classical hydrodynamics problem that still faces many challenges in naval operations,such as cargo transfer and ship pairings between a big transport ship and some small ships.A mathematical model is presented based on the Laplace equation utilizing the porous breakwater to investigate the moored ship motion in a partially absorbing/reflecting harbor.The motion of the moored ship is described with the hydrodynamic forces along the rotational motion(roll,pitch,and yaw)and translational motion(surge,sway,and heave).The efficiency of the numerical method is verified by comparing it with the analytical study of Yu and Chwang(1994)for the porous breakwater,and the moored ship motion is compared with the theoretical and experimental data obtained by Yoo(1998)and Takagi et al.(1993).Further,the current numerical scheme is implemented on the realistic Visakhapatnam Fishing port,India,in order to analyze the hydrodynamic forces on moored ship motion under resonance conditions.The model incorporates some essential strategies such as adding a porous breakwater and utilizing the wave absorber to reduce the port’s resonance.It has been observed that these tactics have a significant impact on the resonance inside the port for safe maritime navigation.Therefore,the current numerical model provides an efficient tool to reduce the resonance within the arbitrarily shaped ports for secure anchoring.展开更多
Mercury （Hg） is present in the marine environment as a natural metal often enhanced through human activities. Depending on its chemical form, Hg can cause a wide range of immunotoxic effects. In this study, the infl...Mercury （Hg） is present in the marine environment as a natural metal often enhanced through human activities. Depending on its chemical form, Hg can cause a wide range of immunotoxic effects. In this study, the influence of methyl-, ethyl- and phenylmercury as well as mercurychloride on immune functions was evaluated. Two parameters of cellular immunity, proliferation and mRNA cytokine expression of interleukin-2, -4, and transforming growth factor β, were investigated in harbor seal lymphocytes after in vitro exposure to Hg compounds. While all Hg compounds had a suppressive effect on proliferation, differences between juvenile and adult seals were found. Lymphocytes from juveniles showed a higher susceptibility to the toxic effect compared to lymphocytes from adults. Furthermore, the degree of inhibition of proliferation varied among the four Hg compounds. The organic compounds seem to be more immunotoxic than the inorganic compound. Finally, for the cytokine expression of methylmercury-incubated lymphocytes, timedependent changes were observed, but no dose-dependency was found. Marine mammals of the North Sea are burdened with Hg, and lymphocytes of harbor seals may be functionally impaired by this metal. The present in vitro study provides baseline information for future studies on the immunotoxic effects of Hg on cellular immunity of harbor seals.展开更多
基金The National Natural Science Foundation of China under contract Nos 42206126 and 42076044the Natural Science Foundation of Shandong Province under contract No.ZR2021QD099.
文摘Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle Harbor over a year,with monthly and quarterly sampling.Both species richness and bacterial numbers exhibited seasonal variations.Specifically,the abundance of attached bacteria and bacterioplankton peaked in June and July,corresponding to higher water temperatures in summer and autumn,while lower abundances were noted in January and December during cooler periods.Throughout the year,the species richness of attached bacteria consistently exceeded that of planktonic bacteria in both shallow and deep waters.Pseudoalteromonas emerged as the most prevalent genus among both planktonic and attached bacteria in surface and bottom seawater samples.Furthermore,the magnitude of changes in species richness and abundance for attached bacteria(0.66×10^(5)-15.85×10^(5)CFU/cm^(2))was greater than that observed for planktonic bacteria(0.58×10^(8)-5.33×10^(8)CFU/L).We propose that the attached bacterial populations,situated in limited microenvironments within the larger seawater ecosystem,exhibit heightened sensitivity to environmental fluctuations,resulting in more rapid shifts in population dynamics and lower ecological stability.The theoretical implications and potential applications of these findings warrant further investigation.
文摘The distribution characteristics of heavy metals such as Cu, Zn, Pb, Cd, Hg. As in seawater, superficail sediments and several marine organisms of Dongzhai Harbor in Hainan were analyzed. Index methods of the single factor and geo-accumulation were used to assess the pollution degree of heavy metals. The results showed that the heavy metal levels in the seawater of Dongzhai Harbor accorded with the first category of the seawater except Pb, and were much lower than the fishery water quality standard; the concentration of the heavy metal in surface sediments did not exceed the national benchmark of the first-class of marine sediment quality basically, and the superficial sediments were essentially uncontaminated; the heavy metal content in wetland sediments was slightly higher than those in other typical mangrove wetland island of Hainan and marine sediments in Dongzhai Haibor, which indicated this area might has been affected by land-based pollution influence, and this should cause the attention of related departments; the heavy metal concentrations in marine organisms were up to the first category. All were not beyond the standard of non-environmental pollution aquatic products.
基金supported by the Study on the Analysis of the Impacts of Reclamation Engineering on Marine Ecological Environment in Yangshan Deepwater Harbor and Protecting Measures of Shanghai Municipal Science and Technology Commission (No. 12231203402)
文摘Temporal and spatial variations of concentrations of heavy metals including mercury （Hg）, zinc （Zn）, lead （Pb）, arsenic （As）, copper （Cu）, and cadmium （Cd） in the sediments of the Yangshan Deepwater Harbor were determined based on 6 cruises in autumn and winter, respectively, from 2010 to 2013. The results demonstrated that the overall concentrations of heavy metals were low and distributed in uniform patterns. The concentrations of Hg, Zn, Pb, and Cd in autumn were significantly higher than those in winter with small fluctuations for As and Cu in terms of seasonal variations. Results of factor analysis showed that Pb, Cd, and Zn were derived from inland industrial and shipping discharges as well as the degradation of organic pollutants in marine environment. While agricultural pollutions, cargo shifting and construction debris from reclamation projects contributed to the sources of Cu, As, and Hg. Ecological risk assessment by Mean Sediment Quality Guideline Quotient （SQG-Q） revealed that the degree for eco-risk of the sediments was low-and-moderate in autumn, higher than that in winter. Hg and Cu were the dominant eco-risk factors. The results of Index of geoaccumulation （Igeo） showed that the whole sites of the sea area were barely influenced by Hg, As, Zn, and Pb, and the extents of Cd and Cu contaminations were in low grade. Contamination degree of all the six heavy metals could be ranked as the following： Cd〉Pb〉Zn〉Hg〉As. According to the results of integrated score of factor analysis, the contamination degree for heavy metals in sediments of the Yangshan Deepwater Harbor was low, despite sites No.5, No.4 and No.3, which were heavily contaminated compared with others.
基金The project is financially supported by the National key Basic Research and Development Program(973 Program,No.2003CB415206)the National Natural Science Foundation of China (Grant No.50379027)
文摘Based on the characteristics of waves, tidal currents, sediment and seabed evolution in the Caofeidian sea area in the Bohai Bay, a 2D sediment mathematical model of waves and tidal currents is employed to study the development schemes of the harbor. Verification of spring and neap tidal currents and sediment in the winter and summer of 2006 shows that the calculated values of tidal stages as well as flow velocities, flow directions and sediment concentration of 15 synchronous vertical lines are in good agreement with the measured data. Also, deposition and erosion of the sea area in front of Caofeidian ore terminal induced by suspended load under tidal currents and waves are verified; it shows that the calculated values of depth of deposition and erosion as well as their distribution are close to the measured data. Furthermore, effects of reclamation scheme of island in front of the land behind Caofeidian harbor on the hydrodynamic environment are studied, including changes of flow velocities in the deep channels at the south side of Caofeidian foreland and Laolonggou and in various harbor basins, as well as changes of deposition and erosion of seabed induced by the project.
基金financially supported by the National Natural Science Foundation of China(Grant No.51209081)NSFC-RS Joint Projects(Grant No.51411130125)+1 种基金Open Foundation of State Key Laboratory of Coastal and Offshore Engineering(Grant No.LP1405)the Fundamental Research Funds for the Central Universities(Grant No.2015B15714)
文摘The general features of oscillations within a rectangular harbor of exponential bottom are investigated analytically. Based on the linear shallow water approximation, analytical solutions for longitudinal oscillations induced by the incident perpendicular wave are obtained by the method of matched asymptotics. The analytic results show that the resonant frequencies are shifted to larger values as the water depth increases and the oscillation amplitudes are enhanced due to the shoaling effect. Owing to the refraction effect, there could be several transverse oscillation modes existing in when the width of the harbor is on the order of the oscillation wavelength. These transverse oscillations are similar to standing edge waves, and there are m node lines in the longshore direction and n node lines running in the offshore direction corresponding to mode（n, m）. Furthermore, the transverse eigen frequency is not only related to the width of the harbor, but also to the boundary condition at the backwall and the bottom shape.
文摘The long-term temporal and spatial dynamics of marine coastal water quality in Tolo Harbor, Hong Kong were explored. The Harbor is divided into three zones represented as Harbor, Buffer, and Channel Subzones. The time range for the study covers the period from the 1970s to the 1990s. The selected indicators for the comprehensive assessment of water quality consist of physical, chemical and biological aspects, including suspended solids(SS), Secchi disk depth(SD), 5-day biochemical oxygen demand(BOD\-5), total nitrogen(TN), total phosphorus(TP), faecal coliform, chlorophyll-a(Chl-a), and the number of red tide occurrences. The results indicated the presence of obvious temporal and spatial trends with regard to changes in water quality. Spatially, water quality in the Channel Subzone is the best, while that in the Harbor Subzone is the worst. On a temporal basis, the average trend from bad to good was 1980s>1990s>1970s as indicated by most of the selected water quality indicators. Water quality during the late 1980s reached its worst level with the lowest SD, the highest BOD\-5, TN, TP, Chl-a concentrations, and the number of red tide occurrences. These long-term temporal-spatial water quality trends were also found in other studies of the Tolo Harbor. The large quantity of pollutants produced as a result of increasing population, industrial and commercial actives, and urbanization and industrialization trends in both Shatin and Tai Po seem to be primarily responsible for the changes in marine coastal water quality.
基金The present work was financially supported by the National Natural Science Foundation of China under contract No.50025924.
文摘A composite numerical model is presented for computing the wave field in a harbor. The mild slope equation is discretized by a finite element method in the domain concerned. Out of the computational domain, the water depth is assumed to be constant. The boundary element method is applied to the outer boundary for dealing with the infinite boundary condition. Because the model satisfies strictly the infinite boundary condition, more accurate results can be obtained. The model is firstly applied to compute the wave diffraction in a narrow rectangular bay and the wave diffraction from a porous cylinder. The numerical results are compared with the analytical solution, experimental data and other numerical results. Good agreements are obtained. Then the model is applied to computing the wave diffraction in a square harbor with varying water depth. The effects of the water depth in the harbor and the incoming wave direction on the wave height distribution are discussed.
基金supported by the Science and Technology Project of the Ministry of Transport(No.2011-329-224-330)the National Nonprofit Institute Research Grants of Tianjin Research Institute for Water Transport Engineering(Nos.TKS100216,TKS130206,and TKS160215),China
文摘A membrane bioreactor （MBR） was established for treating harbor oily wastewater. It showed good removal performance for chemical oxygen demand （COD）, oil content, suspended solids （SS）, and other pollutants. However, serious membrane fouling occurred. It was recognized that the extracellular polymeric substances （EPS） accumulated on the membrane surface, especially the proteins, were of great importance for the transmembrane pressure （TMP） increment and membrane fouling. The MBR was optimized via improving aeration rate and reducing the ratio of Ar/Ad （At and Ad are the cross-sectional areas of the riser and the downcomer of the MBR）. The increasing rate of TMP was slowed, indicating that the optimization strategy could effectively mitigate membrane fouling. Microbial community evolution was monitored and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）, cloning, and sequencing of 16S ribosomal ribonucleic acid （rRNA） fragments. Results revealed that low community shift occurred during the whole operational period. Geobacter sp. and Rhodocyclales sp., which have also been identified by other studies in a petroleum refinery wastewater MBR or an infiltration basin receiving highway runoff, dominated in the MBR system throughout. Comamonas sp. was thought to accommodate the lower aeration rate in this study, while Rhodocyclales sp. preferred the higher aeration rate. In addition, during the operational time under high organic loading rate, a few species were present in abundance, and may have been responsible for the good removal performance at this time.
基金the Atmosphere Protection Plan funded by the Environment, Planning and Housing Regional Agency (Nord-Pas-de-Calais)The “Unité de Chimie Environnementale et Interactions sur le Vivant”, UCEIV EA4492participates in the CLIMIBIO project, which is financially supported by the Hauts-de-France Region Council, the Ministry of Higher Education and Research, the European Regional Development Funds
文摘The influence of in-port ship emissions on gases and PM10 concentrations has been estimated in the port city of Calais, northern France, one of the busiest harbor in Europe, with numerous rotations of ferries or roll-on/roll-off cargo in average per day. NOx, SO2, O3 and PM10 concentrations were continuously measured over a three-month period, as well as real-time particle size distribution. A rural site located at Cape Gris-Nez, 20 km from Calais, was considered to deduce intrinsic contribution of ship emissions at the harbor city. The average concentrations of the studied species as well as the pattern of the conditional bivariate probability function at the two sites evidenced that in-port shipping, especially during the maneuvering operations, has an important influence on the NOx and SO2 concentrations. The impact of shipping in the harbor of Calais on average concentrations was estimated to 5l% for SO2, 35% for NO, 15% for NO2 and 2% for PM10 in the studied period. Concentration peaks of SO2 and NOx associated with an 03 depletion appeared synchronized with departures and arrivals of ferries. For winds blowing from the harbor, when compared to the background level, the number of particles appeared 10 times higher, with the highest differences in the 30-67 nm and the 109-167 nm size ranges. The average impact of in-port ships on PM10 concentrations was estimated to ＋28.9 μg/m^3 and concerned mainly the PM1 size fraction （40%）. Punctually, PM10 can potentially reach a concentration value close to 100μg/m^3.
基金The Key Laboratory Project of State Oceanic Administration for Marine Ecosystem and Biogeochemistry of China under contract No.529101-X21601the Foundation from Wendy Schmidt Ocean Health XPRIZE and the Southern California Coastal Water Research Project
文摘Resulting from the rising levels of atmospheric carbon, ocean acidification has become a global problem. It has significant impacts on the development, survival, growth and physiology of marine organisms. Therefore, a high- precision sensor is urgently needed to measure the pH of sea-water. Iridium wire with a diameter of 0.25 mm is used as the substrate, and an Ir/Ir（OH）x pH electrode is prepared by a one-step electrochemical method in a LiOH solution at the room temperature. A scanning electron microscope （SEM） observation reveals that it is coated with nanoscale particles. In laboratory tests, the electrode exhibits a very promising pH response, with an ideal Nernst slope （56.14-59.52）, fast response, good stability and long fife-span in tested pH buffer solutions. For a sea trial, four pH electrodes and one Ag/AgC1 reference electrode are integrated with a self-made chemical sensor, and a profile detection of nearly 70 m is implemented near Newport Harbor, California on August 3, 2015. The results reflect that the pH value measured by the sensor is very close to the data given by Sea-Bird 911 plus CTD, with a difference value ranging from 0.000 075 to 0.064 719. And the sensor shows a better data matching degree in 0-40 m water depth. In addition, the high precision and accuracy of the sensor make it possible to use in the ocean observation field.
基金financially supported by the National Natural Science Foundation of China (Grant No.51911530205)the Natural Science Foundation of Jiangsu Province (Grant No.BK20201455)+5 种基金the Guangdong Basic and Applied Basic Research Foundation (Grant No.2023A1515010890)the Key Laboratory of PortWaterway and Sedimentation Engineering of MOT (Grant No.YK222001-2)the Open Research Fund of Key Laboratory of Water Security Guarantee in Guangdong-Hong Kong-Marco Greater Bay Area of Ministry of Water Resources (Grant No.WSGBAKJ202309)the Qing Lan Project of Jiangsu Universitiesthe Royal Society (Grant No.IECNSFC181321)。
文摘In this paper,the open-sourced computational fluid dynamics software,OpenFOAM~?,is used to study the fluctuation phenomenon of the water body inside a horizontally one-dimensional enclosed harbor basin with constant water depth triggered by falling wedges with various horizontal falling positions,initial falling velocities and masses.Based on both Fourier transfo rm analysis and wavelet spectrum analysis for the time series of the free surface elevations inside the harbor basin,it is found for the first time that the wedge falling inside the harbor can directly trigger harbor resonance.The influences of the three factors(including the horizontal falling position,the initial falling velocity,and the mass)on the response amplitudes of the lowest three resonant modes are also investigated.The results show that when the wedge falls on one of the nodal points of a resonant mode,the mode would be remarkably suppressed.Conversely,when the wedge falls on one of the anti-nodal points of a resonant mode,the mode would be evidently triggered.The initial falling velocity of the wedge mainly has a remarkable effect on the response amplitude of the most significant mode,and the latter shows a gradual increase trend with the increase of the former.While for the other two less significant modes,their response amplitudes fluctuate around certain constant values as the initial falling velocity rises.In general,the response amplitudes of all the lowest three modes are shown to gradually increase with the mass of the wedge.
基金Key Project of Science and Technology,Ministry of Education, No. 01079
文摘By analysis of published papers on the Yangtze estuary and hydrological and sediments data in Yangshan Harbor area, many similarities are found between Yangshan Harbor area and the Yangtze estuary. These similarities include the phenomenon of stagnating flow areas, the distributive characteristics of the highest suspended sediment concentration areas, superficial sediments and shoal bars. The stagnating flow area is the major similarity which causes other similarities. These similarities indicate that: 1) Turbidity Maximum and mouth bars in estuaries are mainly caused by the hydraulic balance of stagnating flow areas of estuaries; 2) The stagnating sand area of sands caused by stagnating flow area often locates on the narrower side of the stagnating flow area; 3) The location (or shape) of fine sediments area caused by stagnating flow area reflects the location (or shape) of the stagnating flow area. Both Yangshan Harbor area and the Yangtze estuary are the important developmental areas in the future (man-made similarity). In-depth studies on these similarities between Yangshan Harbor area and the Yangtze estuary will have momentous theoretical and practical significance.
基金financially supported by the National Key Research and Development Program of China(Grant No.2017YFC1404200)the National Natural Science Foundation of China(Grant Nos.51911530205 and 51809039)+5 种基金the Natural Science Foundation of Jiangsu Province(Grant No.BK20201455)the Natural Science Foundation of the Jiangsu Higher Education Institutions(Grant No.20KJD170005)the Qing Lan Project of Jiangsu Universitiessupported by UK EPSRC(Grant No.EP/T026782/1)the Royal Academy of Engineering(Grant No.UK-CIAPP/73)the Royal Society(Grant No.IEC\NSFC\181321)。
文摘Influences of topographic variations of the offshore fringing reef on the harbor oscillations excited by incident Nwaves with different amplitudes and waveform types are studied for the first time.Both the propagation of the Nwaves over the reef and the subsequently-induced harbor oscillations are simulated by a Boussinesq-type numerical model,FUNWAVE-TVD.The present study concentrates on revealing the influences of the plane reef-face slope,the reef-face profile shape and the lagoon width on the maximum runup,the wave energy distribution and the total wave energy within the harbor.It shows that both the wave energy distribution uniformity and the total wave energy gradually increase with decreasing reef-face slope.The profile shape of the reef face suffering leading-elevation Nwaves(LEN waves)has a negligible impact on the wave energy distribution uniformity,while for leading-depression N-waves(LDN waves),the latter gradually decreases with the mean water depth over the reef face.The total wave energy always first increases and then decreases with the mean water depth over the reef face.In general,the total wave energy first sharply decreases and then slightly increases with the lagoon width,regardless of the reef-face width and the incident waveform type.The maximum runup subjected to the LEN waves decreases monotonously with the lagoon width.However,for the LDN waves,its changing trend with the lagoon width relies on the incident wave amplitude.
基金The National Natural Science Foundation of China(No.81871203)supported the study.
文摘Dear Editor,Structural aberrations of the Y chromosome,including deletions,ring chromosomes,Y-autosomal or Y-X translocations,isochromosomes,and dicentrics,occur in approximately 0.2%of live births;1 among these,partial deletion in the azoospermia factor(AZF)region of the Y chromosome is a well-characterized cause of nonobstructive severe oligozoospermia and azoospermia.2 Meanwhile,a dicentric Y chromosome,abbreviated as dic(Y),was observed to be exclusively present in males with azoospermia,suggesting more severe negative consequences of the AZF deletion for spermatogenesis.
基金supported by the Major Scientific Research Tasks for Scientific and Technological Innovation Projects of the Chinese Academy of Agricultural Sciences(CAAS-ZDRW202006)the National Transgenic Breeding Project(2018ZX08010-10B)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP-IAS05).
文摘Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent overexpression of foreign genes,without side effects.However,relatively few safe harbor loci are available in pigs,a fact which has impeded the development of multi-transgenic pig research.We report a strategy for efficient transgene knock-in in the endogenous collagen type I alpha 1 chain(COL1A1)gene using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system.After the knock-in of a 2A peptide-green fluorescence protein(2A-GFP)transgene in the last codon of COL1A1 in multiple porcine cells,including porcine kidney epithelial(PK15),porcine embryonic fibroblast(PEF)and porcine intestinal epithelial(IPI-2I)cells,quantitative PCR(qPCR),Western blotting,RNA-seq and CCK8 assay were performed to assess the safety of COL1A1 locus.The qPCR results showed that the GFP knock-in had no effect(P=0.29,P=0.66 and P=0.20 for PK15,PEF and IPI-2I cells,respectively)on the mRNA expression of COL1A1 gene.Similarly,no significant differences(P=0.64,P=0.48 and P=0.80 for PK15,PEF and IPI-2I cells,respectively)were found between the GFP knock-in and wild type cells by Western blotting.RNA-seq results revealed that the transcriptome of GFP knock-in PEF cells had a significant positive correlation(P<2.2e–16)with that of the wild type cells,indicating that the GFP knock-in did not alter the global expression of endogenous genes.Furthermore,the CCK8 assay showed that the GFP knock-in events had no adverse effects(P_(24)h=0.31,P_(48)h=0.96,P_(72)h=0.24,P_(96)h=0.17,and P_(120)h=0.38)on cell proliferation of PK15 cells.These results indicate that the COL1A1 locus can be used as a safe harbor for foreign genes knock-in into the pig genome and can be broadly applied to farm animal breeding and biomedical model establishment.
文摘The motion of the moored ship in the harbor is a classical hydrodynamics problem that still faces many challenges in naval operations,such as cargo transfer and ship pairings between a big transport ship and some small ships.A mathematical model is presented based on the Laplace equation utilizing the porous breakwater to investigate the moored ship motion in a partially absorbing/reflecting harbor.The motion of the moored ship is described with the hydrodynamic forces along the rotational motion(roll,pitch,and yaw)and translational motion(surge,sway,and heave).The efficiency of the numerical method is verified by comparing it with the analytical study of Yu and Chwang(1994)for the porous breakwater,and the moored ship motion is compared with the theoretical and experimental data obtained by Yoo(1998)and Takagi et al.(1993).Further,the current numerical scheme is implemented on the realistic Visakhapatnam Fishing port,India,in order to analyze the hydrodynamic forces on moored ship motion under resonance conditions.The model incorporates some essential strategies such as adding a porous breakwater and utilizing the wave absorber to reduce the port’s resonance.It has been observed that these tactics have a significant impact on the resonance inside the port for safe maritime navigation.Therefore,the current numerical model provides an efficient tool to reduce the resonance within the arbitrarily shaped ports for secure anchoring.
文摘Mercury （Hg） is present in the marine environment as a natural metal often enhanced through human activities. Depending on its chemical form, Hg can cause a wide range of immunotoxic effects. In this study, the influence of methyl-, ethyl- and phenylmercury as well as mercurychloride on immune functions was evaluated. Two parameters of cellular immunity, proliferation and mRNA cytokine expression of interleukin-2, -4, and transforming growth factor β, were investigated in harbor seal lymphocytes after in vitro exposure to Hg compounds. While all Hg compounds had a suppressive effect on proliferation, differences between juvenile and adult seals were found. Lymphocytes from juveniles showed a higher susceptibility to the toxic effect compared to lymphocytes from adults. Furthermore, the degree of inhibition of proliferation varied among the four Hg compounds. The organic compounds seem to be more immunotoxic than the inorganic compound. Finally, for the cytokine expression of methylmercury-incubated lymphocytes, timedependent changes were observed, but no dose-dependency was found. Marine mammals of the North Sea are burdened with Hg, and lymphocytes of harbor seals may be functionally impaired by this metal. The present in vitro study provides baseline information for future studies on the immunotoxic effects of Hg on cellular immunity of harbor seals.
