Handmade cloning （HMC） is the most awaited, simple and micromanipulator-ffee version of somatic cell nuclear transfer （SCNT）. The requirement of expensive micromanipulators and skilled expertise is eliminated in t...Handmade cloning （HMC） is the most awaited, simple and micromanipulator-ffee version of somatic cell nuclear transfer （SCNT）. The requirement of expensive micromanipulators and skilled expertise is eliminated in this technique, proving it as a major revolution in the field of embryology. During the past years, many modifications have been incorporated in this technique to boost its efficiency. This alternative approach to micromanipulator based traditional cloning O-C） works wonder in generating comparable or even higher birth rates in addition to declining costs drastically and enabling cryopreservation. This technique is not only applicable to intraspecies nuclear transfer but also to interspecies nuclear transfer （iSCNT） thus permitting conservation of endangered species. It also offers unique possibilities for automation of SCNT which aims at production of transgenic animals that can cure certain human diseases by producing therapeutics hence, providing a healthier future for the wellbeing of humans. The present review aims at highlighting certain aspects of HMC including recent advancements in procedure and factors involved in elevating its efficiency besides covering the potentials and pitfalls of this technique展开更多
Chinese traditional handmade papers,due to their numerous names,have been misunderstood by the public about raw materials for a long time.This manuscript aims to introduce the raw materials of traditional Chinese hand...Chinese traditional handmade papers,due to their numerous names,have been misunderstood by the public about raw materials for a long time.This manuscript aims to introduce the raw materials of traditional Chinese handmade paper in a botanical aspect in chronological order.Chinese handmade paper’s raw materials are divided into two groups,bast fiber and grass fiber.Bast fiber paper includes ramie-made rag paper,mulberry and paper mulberry paper,Thymelaeaceae paper,and vine paper.Stalk and grass fiber paper includes bamboo paper,grass paper,and stalk paper.展开更多
Both Edgeworthia and Wikstroemia have been used traditionally in hand papermaking in China.As plants from the Thymelaeaceae family,these species have been mentioned periodically and have been mixed or substituted with...Both Edgeworthia and Wikstroemia have been used traditionally in hand papermaking in China.As plants from the Thymelaeaceae family,these species have been mentioned periodically and have been mixed or substituted with each other.This study reviewed their papermaking background and status,and selected one of the Edgeworthia and three of the Wikstroemia raw materials that are still in use.Pulp was cooked under the same conditions in the laboratory,and handsheets of pure and mixed materials were obtained using a Rapid-Köthen sheet former.A series of physical properties,such as color,structure,and mechanical properties were tested,followed by a comparison and discussion of the raw material properties.The results show that Wikstroemia fibers are generally thinner and shorter,but there are variations between the different genera of raw materials.Thus,compared with Edgeworthia,Wikstroemia has some advantages in tensile strength,although it shows some disadvantages when it comes to tearing strength.The performance of a mixed-ingredient paper sheet falls between the performance of two pure-component papers.For cost and performance reasons,adding a small amount of Wikstroemia(e.g.,<20%)to Edgeworthia is preferable.展开更多
The mulberry paper handmade dryer uses downdraft gasifier, which is a continuous hot air dryer. The downdraft gasifier uses charcoal or wood chip as fuel to produce the producer gas for a dryer heat source. Two steps ...The mulberry paper handmade dryer uses downdraft gasifier, which is a continuous hot air dryer. The downdraft gasifier uses charcoal or wood chip as fuel to produce the producer gas for a dryer heat source. Two steps operation of a dryer as follows： The frst was to reduce mulberry paper pulp moisture by an air vacuum pump; the second was a continuous hot air drying process. The optimum condition drying, the capacity of dryer, the fuel consumption, the drying constant （k） and economics analysis were investigated. It was found that the first step could be to reduce mulberry paper pulp moisture content about 25% and the suitable condition drying was 80 ~C drying temperature, 0.04 kg/s air mass flow rate and 0.29 m/min chain conveyor speed, respectively. The capacity of this dryer was 20 sheets per hour. The quality of mulberry paper product was very good （based on the standard of mulberry paper community 41/2546） and the fuel consumption rate was 5 kg/h （charcoal）. The drying constant was about 0.532933-0.541367 min~ and the drying constant was a function of drying temperature （T）, air mass flow rate （F） and conveyor speed （10 as equation of k（T, F, V） = 0.567494 ＋ 0.000422T- 1.40588F- 0.000205 V （R2 = 0.9254） and the breakeven point of dryer was 0.79 years.展开更多
Effects of different enucleation methods on developmental potency of pig handmade clone（HMC）reconstructed embryo was investigated in the paper.We compared enucleation efficiency of blind cut method,first polar body...Effects of different enucleation methods on developmental potency of pig handmade clone（HMC）reconstructed embryo was investigated in the paper.We compared enucleation efficiency of blind cut method,first polar body（Pb1）positioning method and demecolcine（DM）assisted enucleation,as well as their effects on development of HMC reconstructed embryos.The results showed that overall enucleation efficiency of Pb1 positioning method was significantly higher than that of blind cut method（P〈0.05）.The protuberance rate and overall enucleation efficiency of 0.4μg/mL DM treated group for 60 min was significantly higher than that of other concentrations and time treatment groups（P〈0.05）.For effects on development of HMC reconstructed embryos,there was no significant difference between DM-assisted enucleation and Pb1 positioning method.In conclusion,appropriate addition of DM could enhance enucleation efficiency of HMC,which had no significant influence on developmental potency of reconstructed embryos.展开更多
The sound of loom weaving comes out of a three-story building in the Lugu Alley off the Barkor Street.Drugyal’s wife Norbu Droma and her assistants are making traditional Tibetan bags.The black pulu shop sign stands ...The sound of loom weaving comes out of a three-story building in the Lugu Alley off the Barkor Street.Drugyal’s wife Norbu Droma and her assistants are making traditional Tibetan bags.The black pulu shop sign stands out in the quiet alley.Step into the shop,you will see wool,calfskin and sheepskin bags hang neatly on the wall.The products on display on the counter are of the current style made for everyday life,while those traditional handmade bags in the展开更多
This study aims to explore the potential of using a blended pulp from Mikania micrantha(M.micrantha)and waste paper for producing composite paper.The effects of the mass ratio of M.micrantha stem to waste paper(MRMW),...This study aims to explore the potential of using a blended pulp from Mikania micrantha(M.micrantha)and waste paper for producing composite paper.The effects of the mass ratio of M.micrantha stem to waste paper(MRMW),the beating time(BT),the water-to-pulp mass ratio(WPMR)and the times of pulp suspension screening(TPSS)on the paper’s basic structural,optical and mechanical properties are investigated.It is found that MRMW primarily affects the grammage(mass per unit area),density,bulkness and whiteness;WPMR mainly affects the thickness and density;TPSS mainly affects the thickness and grammage.When MRMW is 3:7,the composite paper shows higher values for thickness,grammage,density and whiteness;whereas when MRMW is 7:3,these values are lower.Extending BT can increase paper density.The tensile strengths of all prepared samples fall in the range of 1.5 to 4.1 kN/m,indicating their excellent strength properties that meet the demands of many paper applications.The artistic bags and lampshades crafted from this composite paper exhibit a more natural texture compared to conventional packaging paper.This research demonstrates the feasibility of papermaking by using M.micrantha,while showcasing the potential for synergistic integration of waste resources with traditional hand papermaking techniques.展开更多
A 52-year-old female presented to our clinic after accidentallyingesting a push-through pack(PTP). After determining that the PTP was present in the stomach, we successfully and safely removed it endoscopically by usi...A 52-year-old female presented to our clinic after accidentallyingesting a push-through pack(PTP). After determining that the PTP was present in the stomach, we successfully and safely removed it endoscopically by using a handmade endoscopic hood fashioned from a cut endotracheal tube. Foreign body ingestion is a common clinical problem, and most ingested foreign bodies pass spontaneously. However, the ingestion of sharp objects, such as PTPs, increases the risk of complications, and urgent endoscopy is recommended to remove such objects. Previous studies have reported the use of other devices, both commercial and handmade, for the safe endoscopic removal of foreign bodies. The novel design of our handmade hood for the removal of the PTP, which was fashioned from a cut endotracheal tube, was beneficial in terms of maintaining a wide visual field, patient safety and tolerance, and easy preparation compared to previously reported commercial and handmade devices. It may be a viable and safe device for the retrieval of PTPs and other sharp foreign bodies.展开更多
In recent years, in order to better develop basic education, the continuous education reform in primary and secondary schools has been constantly moving closer to quality education and committed to the cultivation of ...In recent years, in order to better develop basic education, the continuous education reform in primary and secondary schools has been constantly moving closer to quality education and committed to the cultivation of students comprehensive quality, so as to help students lay a foundation for learning. After the implementation of the "double reduction policy", compulsory education in all regions has added after-school delay services which are used to guide students in their homework, study and implement the expansion and extension of classroom teaching. For the development of education in primary school, students have less learning pressure and burden. We should make full use of after-school services, increase practical activities in combination with pupils physical and mental development laws, and develop pupils abilities. This article mainly discusses the strategy of integrating handmade courses into after-school service teaching under the background of "double reduction", hoping to make full use of after-school services and mobilize the enthusiasm of students.展开更多
文摘Handmade cloning （HMC） is the most awaited, simple and micromanipulator-ffee version of somatic cell nuclear transfer （SCNT）. The requirement of expensive micromanipulators and skilled expertise is eliminated in this technique, proving it as a major revolution in the field of embryology. During the past years, many modifications have been incorporated in this technique to boost its efficiency. This alternative approach to micromanipulator based traditional cloning O-C） works wonder in generating comparable or even higher birth rates in addition to declining costs drastically and enabling cryopreservation. This technique is not only applicable to intraspecies nuclear transfer but also to interspecies nuclear transfer （iSCNT） thus permitting conservation of endangered species. It also offers unique possibilities for automation of SCNT which aims at production of transgenic animals that can cure certain human diseases by producing therapeutics hence, providing a healthier future for the wellbeing of humans. The present review aims at highlighting certain aspects of HMC including recent advancements in procedure and factors involved in elevating its efficiency besides covering the potentials and pitfalls of this technique
文摘Chinese traditional handmade papers,due to their numerous names,have been misunderstood by the public about raw materials for a long time.This manuscript aims to introduce the raw materials of traditional Chinese handmade paper in a botanical aspect in chronological order.Chinese handmade paper’s raw materials are divided into two groups,bast fiber and grass fiber.Bast fiber paper includes ramie-made rag paper,mulberry and paper mulberry paper,Thymelaeaceae paper,and vine paper.Stalk and grass fiber paper includes bamboo paper,grass paper,and stalk paper.
基金supported by the National Key R&D Program of China(2019YFC1520300).
文摘Both Edgeworthia and Wikstroemia have been used traditionally in hand papermaking in China.As plants from the Thymelaeaceae family,these species have been mentioned periodically and have been mixed or substituted with each other.This study reviewed their papermaking background and status,and selected one of the Edgeworthia and three of the Wikstroemia raw materials that are still in use.Pulp was cooked under the same conditions in the laboratory,and handsheets of pure and mixed materials were obtained using a Rapid-Köthen sheet former.A series of physical properties,such as color,structure,and mechanical properties were tested,followed by a comparison and discussion of the raw material properties.The results show that Wikstroemia fibers are generally thinner and shorter,but there are variations between the different genera of raw materials.Thus,compared with Edgeworthia,Wikstroemia has some advantages in tensile strength,although it shows some disadvantages when it comes to tearing strength.The performance of a mixed-ingredient paper sheet falls between the performance of two pure-component papers.For cost and performance reasons,adding a small amount of Wikstroemia(e.g.,<20%)to Edgeworthia is preferable.
文摘The mulberry paper handmade dryer uses downdraft gasifier, which is a continuous hot air dryer. The downdraft gasifier uses charcoal or wood chip as fuel to produce the producer gas for a dryer heat source. Two steps operation of a dryer as follows： The frst was to reduce mulberry paper pulp moisture by an air vacuum pump; the second was a continuous hot air drying process. The optimum condition drying, the capacity of dryer, the fuel consumption, the drying constant （k） and economics analysis were investigated. It was found that the first step could be to reduce mulberry paper pulp moisture content about 25% and the suitable condition drying was 80 ~C drying temperature, 0.04 kg/s air mass flow rate and 0.29 m/min chain conveyor speed, respectively. The capacity of this dryer was 20 sheets per hour. The quality of mulberry paper product was very good （based on the standard of mulberry paper community 41/2546） and the fuel consumption rate was 5 kg/h （charcoal）. The drying constant was about 0.532933-0.541367 min~ and the drying constant was a function of drying temperature （T）, air mass flow rate （F） and conveyor speed （10 as equation of k（T, F, V） = 0.567494 ＋ 0.000422T- 1.40588F- 0.000205 V （R2 = 0.9254） and the breakeven point of dryer was 0.79 years.
基金Supported by National Natural Science Foundation of China(31360544)
文摘Effects of different enucleation methods on developmental potency of pig handmade clone（HMC）reconstructed embryo was investigated in the paper.We compared enucleation efficiency of blind cut method,first polar body（Pb1）positioning method and demecolcine（DM）assisted enucleation,as well as their effects on development of HMC reconstructed embryos.The results showed that overall enucleation efficiency of Pb1 positioning method was significantly higher than that of blind cut method（P〈0.05）.The protuberance rate and overall enucleation efficiency of 0.4μg/mL DM treated group for 60 min was significantly higher than that of other concentrations and time treatment groups（P〈0.05）.For effects on development of HMC reconstructed embryos,there was no significant difference between DM-assisted enucleation and Pb1 positioning method.In conclusion,appropriate addition of DM could enhance enucleation efficiency of HMC,which had no significant influence on developmental potency of reconstructed embryos.
文摘The sound of loom weaving comes out of a three-story building in the Lugu Alley off the Barkor Street.Drugyal’s wife Norbu Droma and her assistants are making traditional Tibetan bags.The black pulu shop sign stands out in the quiet alley.Step into the shop,you will see wool,calfskin and sheepskin bags hang neatly on the wall.The products on display on the counter are of the current style made for everyday life,while those traditional handmade bags in the
文摘This study aims to explore the potential of using a blended pulp from Mikania micrantha(M.micrantha)and waste paper for producing composite paper.The effects of the mass ratio of M.micrantha stem to waste paper(MRMW),the beating time(BT),the water-to-pulp mass ratio(WPMR)and the times of pulp suspension screening(TPSS)on the paper’s basic structural,optical and mechanical properties are investigated.It is found that MRMW primarily affects the grammage(mass per unit area),density,bulkness and whiteness;WPMR mainly affects the thickness and density;TPSS mainly affects the thickness and grammage.When MRMW is 3:7,the composite paper shows higher values for thickness,grammage,density and whiteness;whereas when MRMW is 7:3,these values are lower.Extending BT can increase paper density.The tensile strengths of all prepared samples fall in the range of 1.5 to 4.1 kN/m,indicating their excellent strength properties that meet the demands of many paper applications.The artistic bags and lampshades crafted from this composite paper exhibit a more natural texture compared to conventional packaging paper.This research demonstrates the feasibility of papermaking by using M.micrantha,while showcasing the potential for synergistic integration of waste resources with traditional hand papermaking techniques.
文摘A 52-year-old female presented to our clinic after accidentallyingesting a push-through pack(PTP). After determining that the PTP was present in the stomach, we successfully and safely removed it endoscopically by using a handmade endoscopic hood fashioned from a cut endotracheal tube. Foreign body ingestion is a common clinical problem, and most ingested foreign bodies pass spontaneously. However, the ingestion of sharp objects, such as PTPs, increases the risk of complications, and urgent endoscopy is recommended to remove such objects. Previous studies have reported the use of other devices, both commercial and handmade, for the safe endoscopic removal of foreign bodies. The novel design of our handmade hood for the removal of the PTP, which was fashioned from a cut endotracheal tube, was beneficial in terms of maintaining a wide visual field, patient safety and tolerance, and easy preparation compared to previously reported commercial and handmade devices. It may be a viable and safe device for the retrieval of PTPs and other sharp foreign bodies.
文摘In recent years, in order to better develop basic education, the continuous education reform in primary and secondary schools has been constantly moving closer to quality education and committed to the cultivation of students comprehensive quality, so as to help students lay a foundation for learning. After the implementation of the "double reduction policy", compulsory education in all regions has added after-school delay services which are used to guide students in their homework, study and implement the expansion and extension of classroom teaching. For the development of education in primary school, students have less learning pressure and burden. We should make full use of after-school services, increase practical activities in combination with pupils physical and mental development laws, and develop pupils abilities. This article mainly discusses the strategy of integrating handmade courses into after-school service teaching under the background of "double reduction", hoping to make full use of after-school services and mobilize the enthusiasm of students.
