Haloarchaea represents a unique group of microorganisms that have adapted to thrive in high-salt environments.These microbes produce distinctive biomolecules,some of which exhibit extraordinary properties.One such bio...Haloarchaea represents a unique group of microorganisms that have adapted to thrive in high-salt environments.These microbes produce distinctive biomolecules,some of which exhibit extraordinary properties.One such biomolecule is bacterioruberin,a prominent red-pigmented C50 carotenoid commonly found in halophilic archaea,renowned for its antioxidant properties and potential as a functional resource.This study aimed to enhance the culture conditions for optimal production of C50 carotenoids,primarily bacterioruberin,using“Haloferax marinum”MBLA0078.The optimization process involved a combination of one-factor-at-a-time(OFAT)and statistical methodology.Under OFAT-optimized conditions,fed-batch fermentation,and response surface methodology(RSM)optimization,carotenoid production reached 0.954 mg/L,2.80 mg/L,and 2.16 mg/L,respectively,in a 7-L laboratory-scale fermenter.Notably,RSM-optimized conditions led to a 12-fold increase in productivity(0.72 mg/L/day)compared to the basal DBCM2 medium(0.06 mg/L/day).These findings suggest that strain MBLA0078 holds significant promise for commercial-scale production of bacterioruberin.展开更多
Skin cancer, including both melanoma and non-melanoma, is the most common type of malignancy, which causes substantial morbidities and mortalities. Although the significant increase in the understanding of skin cancer...Skin cancer, including both melanoma and non-melanoma, is the most common type of malignancy, which causes substantial morbidities and mortalities. Although the significant increase in the understanding of skin cancer formation and the development of novel personalized drug regimens have occurred, new treatment options are always of need. The use of natural compounds to alleviate the symptoms or even to prevent and treat cancer has long been proposed. Specifically, the use of marine-based organisms as a source for cancer cure and remedy is being evaluated extensively. The objective of the current study was to assess the ability of the green microalgae Dunaliella salina, the Dead-Sea-derived Haloferax volcanii, and its combinations to treat skin cancer in vitro. The results demonstrate the Dunaliella and Haloferax can reduce sarcoma and basal cell carcinoma cellular growth. Importantly, their combination acts synergistically in a caspase-3 independent manner. Moreover, a synergistic action was found when evaluated sarcoma cell invasion rate, which was completely blocked at pharmacological relevant amounts of the compounds. Collectively, the results demonstrate that the combination of Haloferax volcanii and Dunaliella salina can be used as a new treatment for skin cancer. The specific mechanism of action and further in vivo validation studies are of need.展开更多
The stationary phase of microbial growth is a very complex state regulated by various environmental and physiological factors. An intensive study of stationary phase could promote a comprehensive understanding of the ...The stationary phase of microbial growth is a very complex state regulated by various environmental and physiological factors. An intensive study of stationary phase could promote a comprehensive understanding of the complete life cycle of microorganisms, and may provide important insights into their adaptation to harsh and nutrient-depleted conditions. Although the underlying mechanisms have been weU-studied in bacteria and yeasts （Herman, 2002; Navarro Llorens et al., 2010）, less is known about this growth phase in archaea yet. The haloarchaeon Haloferax mediterranei has served as a good model for studying haloarchaeal physiology and metabolism for several decades because of its accelerated growth, remarkable metabolic ability and genomic stability （Han et al., 2012）. During stationary phase, H. mediterranei can produce halocin H4 （Cheung et al.,展开更多
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(2022R1F1A1062699).
文摘Haloarchaea represents a unique group of microorganisms that have adapted to thrive in high-salt environments.These microbes produce distinctive biomolecules,some of which exhibit extraordinary properties.One such biomolecule is bacterioruberin,a prominent red-pigmented C50 carotenoid commonly found in halophilic archaea,renowned for its antioxidant properties and potential as a functional resource.This study aimed to enhance the culture conditions for optimal production of C50 carotenoids,primarily bacterioruberin,using“Haloferax marinum”MBLA0078.The optimization process involved a combination of one-factor-at-a-time(OFAT)and statistical methodology.Under OFAT-optimized conditions,fed-batch fermentation,and response surface methodology(RSM)optimization,carotenoid production reached 0.954 mg/L,2.80 mg/L,and 2.16 mg/L,respectively,in a 7-L laboratory-scale fermenter.Notably,RSM-optimized conditions led to a 12-fold increase in productivity(0.72 mg/L/day)compared to the basal DBCM2 medium(0.06 mg/L/day).These findings suggest that strain MBLA0078 holds significant promise for commercial-scale production of bacterioruberin.
文摘Skin cancer, including both melanoma and non-melanoma, is the most common type of malignancy, which causes substantial morbidities and mortalities. Although the significant increase in the understanding of skin cancer formation and the development of novel personalized drug regimens have occurred, new treatment options are always of need. The use of natural compounds to alleviate the symptoms or even to prevent and treat cancer has long been proposed. Specifically, the use of marine-based organisms as a source for cancer cure and remedy is being evaluated extensively. The objective of the current study was to assess the ability of the green microalgae Dunaliella salina, the Dead-Sea-derived Haloferax volcanii, and its combinations to treat skin cancer in vitro. The results demonstrate the Dunaliella and Haloferax can reduce sarcoma and basal cell carcinoma cellular growth. Importantly, their combination acts synergistically in a caspase-3 independent manner. Moreover, a synergistic action was found when evaluated sarcoma cell invasion rate, which was completely blocked at pharmacological relevant amounts of the compounds. Collectively, the results demonstrate that the combination of Haloferax volcanii and Dunaliella salina can be used as a new treatment for skin cancer. The specific mechanism of action and further in vivo validation studies are of need.
基金supported by the grants from the National Natural Science Foundation of China(Nos.30925001 and 31271334)the Chinese Academy of Sciences(No. KSCX2-EW-G-2-4)
文摘The stationary phase of microbial growth is a very complex state regulated by various environmental and physiological factors. An intensive study of stationary phase could promote a comprehensive understanding of the complete life cycle of microorganisms, and may provide important insights into their adaptation to harsh and nutrient-depleted conditions. Although the underlying mechanisms have been weU-studied in bacteria and yeasts （Herman, 2002; Navarro Llorens et al., 2010）, less is known about this growth phase in archaea yet. The haloarchaeon Haloferax mediterranei has served as a good model for studying haloarchaeal physiology and metabolism for several decades because of its accelerated growth, remarkable metabolic ability and genomic stability （Han et al., 2012）. During stationary phase, H. mediterranei can produce halocin H4 （Cheung et al.,
