BACKGROUND Heterozygous familial hypobetalipoproteinemia(FHBL)is a semi-autosomal disorder that is caused mainly by an APOB variant.It is usually asymptomatic and rarely leads to non-alcoholic steatohepatitis(NASH).CA...BACKGROUND Heterozygous familial hypobetalipoproteinemia(FHBL)is a semi-autosomal disorder that is caused mainly by an APOB variant.It is usually asymptomatic and rarely leads to non-alcoholic steatohepatitis(NASH).CASE SUMMARY A 12-year-old boy was referred to our hospital after prolonged elevation of liver enzymes was observed during health checkups in Kagawa Prefecture.Abdominal ultrasound showed a bright liver,and laboratory investigations revealed low lowdensity lipoprotein cholesterol and apolipoprotein B protein levels.His family history included fatty liver and hypolipidemia in his father,which led to a clinical diagnosis of FHBL.A liver biopsy was performed on suspicion of liver fibrosis based on biomarkers.The liver tissue showed fatty steatosis,inflammation,hepatocyte ballooning,and fibrosis,indicating NASH.Genetic testing detected the APOB variant,and the patient was treated successfully with vitamin E.CONCLUSION It is important to assess family history and liver dysfunction severity in non-obese patients with hypolipidemia and fatty liver.展开更多
目的评价微藻油对高脂饮食(high-fatdiet,HFD)诱导的大鼠高血脂的影响。方法选用70只4w龄SPF级雄性SD大鼠,随机分为高脂饮食组(n=50)和正常饮食组(n=20),分别给予高脂饲料和正常饲料喂养3w后,按照血清总胆固醇水平将高脂饮食组随机分为...目的评价微藻油对高脂饮食(high-fatdiet,HFD)诱导的大鼠高血脂的影响。方法选用70只4w龄SPF级雄性SD大鼠,随机分为高脂饮食组(n=50)和正常饮食组(n=20),分别给予高脂饲料和正常饲料喂养3w后,按照血清总胆固醇水平将高脂饮食组随机分为模型组、微藻油低、中、高干预组及阳性对照组,将正常饮食组分为对照组及微藻油高剂量对照组,对照组及模型组灌胃玉米油,微藻油低、中、高剂量组分别灌胃300、600、1800 mg/kg的微藻油,阳性对照组灌胃1mg/kg的阿托伐他汀钙片,各组饲料保持不变;干预5 w后,麻醉大鼠,腹主动脉取血后处死,分别检测大鼠血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)等指标,取各组大鼠肝脏、肾脏、颈动脉进行组织病理学检查。结果干预5w后,高脂饲料喂养的各组大鼠体重无显著差异(P>0.05);与模型组相比,微藻油中、高剂量干预组及阳性对照组TC、TG、LDL-C均显著降低(P<0.05),HDL-C显著升高(P<0.05);与干预前相比,干预后微藻油低、中、高剂量干预组TC分别降低了3.10%、9.9%、24.8%,TG分别降低了2.7%、5.4%、10.3%,LDL-C分别降低了3.4%、16.6%、18.4%,HDL-C分别升高了16.7%、28.2%、30.7%。此外,与模型组相比,中、高剂量干预组谷草转氨酶、谷丙转氨酶、肌酐及尿素氮水平均显著降低(P<0.05);而与对照组相比,高剂量对照组上述指标均无显著差异(P>0.05);病理学结果显示:对照组与高剂量对照组肝脏、肾脏结构正常,模型组肝脏出现脂肪化,肾脏组织出现肾小管细胞肿大、排列紊乱等病理改变,微藻油不同剂量干预组均有不同程度的改善。结论微藻油在特定剂量范围内具有辅助降血脂的功效。展开更多
Previous study demonstrated that Ganoderma meroterpene derivative(GMD)increased the abundance of butyrate-producing bacteria in gut and subsequently delivered anti-metabolic disorder effect of host.To specify the key ...Previous study demonstrated that Ganoderma meroterpene derivative(GMD)increased the abundance of butyrate-producing bacteria in gut and subsequently delivered anti-metabolic disorder effect of host.To specify the key commensal bacteria associating with the beneficial effects,we tried to isolate and compare the microbiota from the cecal samples of GMD-and vehicle-treated ob/ob mice,and further identified butyrate-producing bacterial strains.It was found that Faeciroseburia intestinalis was enriched and 11 strains affiliated to F.intestinalis were cultivated from the gut of GMD-treated mice.In vitro assay attested butyrate production by representative strain of F.intestinalis.Oral administration with F.intestinalis further demonstrated its benefits on regulating hyperglycemia and hyperlipidemia,on decreasing plasma lipopolysaccharide(LPS)and inflammation,and on improving hepatic injuries.Treatment with F.intestinalis effectively enhanced the level of gut butyrate,which subsequently ameliorated the intestinal barrier function and activated epithelial PPAR-γ signaling pathway to regulate microbiome homeostasis in gut.Our study demonstrated that the causal relationship between the butyrate-producing bacteria and the GMD's therapeutic effects and confirmed the important function of the butyrate-producing F.intestinalis in maintaining host metabolism homeostasis.展开更多
The study was carried out to evaluate the hypolipidemic and hypoglycemic effect of Nelumbo nucifera leaf powder. In rats, hyperlipidemia was induced by feeding High Fat Diet (Lab diet: Dalda: Coconut oil = 4:3:1) and ...The study was carried out to evaluate the hypolipidemic and hypoglycemic effect of Nelumbo nucifera leaf powder. In rats, hyperlipidemia was induced by feeding High Fat Diet (Lab diet: Dalda: Coconut oil = 4:3:1) and type 2 diabetes was built inside by injecting Alloxan. The sample N. nucifera leaf powder was added in different percentages with the regular Lab Diet for 21 days feeding. In case of hyperlipidemia developed groups, feeding of Nelumbo nucifera leaf powder at, 30% (70% Lab diet + 30% Sample), 20% (80% Lab diet + 20% Sample) and 10% (90% Lab diet + 10% Sample) showed varied but in a nutshell significant (p Nelumbo nucifera leaf powder for 21 days resulted significant decrease in the blood glucose levels of alloxan-induced diabetic rats. Both the percentages of Nelumbo nucifera leaf powder having 20% with 80% Lab Diet and 10% with 90% Lab Diet significantly (p Nelumbo nucifera powder was found to be exerted to the more prominent effects in lowering the blood glucose level. Nelumbo nucifera leaf powder had 75% efficacy rate in lowering the blood glucose level in comparison to the drug control group treated with glibenclamide which was found with the decreasing capability up to 66%. Although not significant, it, based on the data, can be stated that the sample is endowed with the quality of decreasing capability of body weight. Thus, results of the experimental study reveal that the leaf of Nelumbo nucifera has potent hypoglycemic and hypolipidemic properties.展开更多
文摘BACKGROUND Heterozygous familial hypobetalipoproteinemia(FHBL)is a semi-autosomal disorder that is caused mainly by an APOB variant.It is usually asymptomatic and rarely leads to non-alcoholic steatohepatitis(NASH).CASE SUMMARY A 12-year-old boy was referred to our hospital after prolonged elevation of liver enzymes was observed during health checkups in Kagawa Prefecture.Abdominal ultrasound showed a bright liver,and laboratory investigations revealed low lowdensity lipoprotein cholesterol and apolipoprotein B protein levels.His family history included fatty liver and hypolipidemia in his father,which led to a clinical diagnosis of FHBL.A liver biopsy was performed on suspicion of liver fibrosis based on biomarkers.The liver tissue showed fatty steatosis,inflammation,hepatocyte ballooning,and fibrosis,indicating NASH.Genetic testing detected the APOB variant,and the patient was treated successfully with vitamin E.CONCLUSION It is important to assess family history and liver dysfunction severity in non-obese patients with hypolipidemia and fatty liver.
文摘目的评价微藻油对高脂饮食(high-fatdiet,HFD)诱导的大鼠高血脂的影响。方法选用70只4w龄SPF级雄性SD大鼠,随机分为高脂饮食组(n=50)和正常饮食组(n=20),分别给予高脂饲料和正常饲料喂养3w后,按照血清总胆固醇水平将高脂饮食组随机分为模型组、微藻油低、中、高干预组及阳性对照组,将正常饮食组分为对照组及微藻油高剂量对照组,对照组及模型组灌胃玉米油,微藻油低、中、高剂量组分别灌胃300、600、1800 mg/kg的微藻油,阳性对照组灌胃1mg/kg的阿托伐他汀钙片,各组饲料保持不变;干预5 w后,麻醉大鼠,腹主动脉取血后处死,分别检测大鼠血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)等指标,取各组大鼠肝脏、肾脏、颈动脉进行组织病理学检查。结果干预5w后,高脂饲料喂养的各组大鼠体重无显著差异(P>0.05);与模型组相比,微藻油中、高剂量干预组及阳性对照组TC、TG、LDL-C均显著降低(P<0.05),HDL-C显著升高(P<0.05);与干预前相比,干预后微藻油低、中、高剂量干预组TC分别降低了3.10%、9.9%、24.8%,TG分别降低了2.7%、5.4%、10.3%,LDL-C分别降低了3.4%、16.6%、18.4%,HDL-C分别升高了16.7%、28.2%、30.7%。此外,与模型组相比,中、高剂量干预组谷草转氨酶、谷丙转氨酶、肌酐及尿素氮水平均显著降低(P<0.05);而与对照组相比,高剂量对照组上述指标均无显著差异(P>0.05);病理学结果显示:对照组与高剂量对照组肝脏、肾脏结构正常,模型组肝脏出现脂肪化,肾脏组织出现肾小管细胞肿大、排列紊乱等病理改变,微藻油不同剂量干预组均有不同程度的改善。结论微藻油在特定剂量范围内具有辅助降血脂的功效。
基金supported by the National Key R&D program of China(2019YFA0905602)the National Natural Science Foundation of China(81773614)the Strategic Priority Research Program of Chinese Academy of Sciences(grant No.XDB 38020300).
文摘Previous study demonstrated that Ganoderma meroterpene derivative(GMD)increased the abundance of butyrate-producing bacteria in gut and subsequently delivered anti-metabolic disorder effect of host.To specify the key commensal bacteria associating with the beneficial effects,we tried to isolate and compare the microbiota from the cecal samples of GMD-and vehicle-treated ob/ob mice,and further identified butyrate-producing bacterial strains.It was found that Faeciroseburia intestinalis was enriched and 11 strains affiliated to F.intestinalis were cultivated from the gut of GMD-treated mice.In vitro assay attested butyrate production by representative strain of F.intestinalis.Oral administration with F.intestinalis further demonstrated its benefits on regulating hyperglycemia and hyperlipidemia,on decreasing plasma lipopolysaccharide(LPS)and inflammation,and on improving hepatic injuries.Treatment with F.intestinalis effectively enhanced the level of gut butyrate,which subsequently ameliorated the intestinal barrier function and activated epithelial PPAR-γ signaling pathway to regulate microbiome homeostasis in gut.Our study demonstrated that the causal relationship between the butyrate-producing bacteria and the GMD's therapeutic effects and confirmed the important function of the butyrate-producing F.intestinalis in maintaining host metabolism homeostasis.
文摘The study was carried out to evaluate the hypolipidemic and hypoglycemic effect of Nelumbo nucifera leaf powder. In rats, hyperlipidemia was induced by feeding High Fat Diet (Lab diet: Dalda: Coconut oil = 4:3:1) and type 2 diabetes was built inside by injecting Alloxan. The sample N. nucifera leaf powder was added in different percentages with the regular Lab Diet for 21 days feeding. In case of hyperlipidemia developed groups, feeding of Nelumbo nucifera leaf powder at, 30% (70% Lab diet + 30% Sample), 20% (80% Lab diet + 20% Sample) and 10% (90% Lab diet + 10% Sample) showed varied but in a nutshell significant (p Nelumbo nucifera leaf powder for 21 days resulted significant decrease in the blood glucose levels of alloxan-induced diabetic rats. Both the percentages of Nelumbo nucifera leaf powder having 20% with 80% Lab Diet and 10% with 90% Lab Diet significantly (p Nelumbo nucifera powder was found to be exerted to the more prominent effects in lowering the blood glucose level. Nelumbo nucifera leaf powder had 75% efficacy rate in lowering the blood glucose level in comparison to the drug control group treated with glibenclamide which was found with the decreasing capability up to 66%. Although not significant, it, based on the data, can be stated that the sample is endowed with the quality of decreasing capability of body weight. Thus, results of the experimental study reveal that the leaf of Nelumbo nucifera has potent hypoglycemic and hypolipidemic properties.
