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BETWEEN HONOR AND HUMILIATION:——STARTING FROM THE RIGHTS AND WRONGS BY FOREIGN INVESTMENT(Ⅲ)
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作者 Ma Yu 《大经贸》 北大核心 1997年第6期90-92,共3页
The Poor Becomes Even Poorer, And TheRich Becomes Even Richer-The Mat-thew Effect In Foreign Investmenthe nature of the capital is keen on the rich but cold-shoul-der the poor. If you are rich, dollars, pounds or RMBs... The Poor Becomes Even Poorer, And TheRich Becomes Even Richer-The Mat-thew Effect In Foreign Investmenthe nature of the capital is keen on the rich but cold-shoul-der the poor. If you are rich, dollars, pounds or RMBs wouldgreet you with a genial smile. If you are poor, you will beignored. Maybe, you will be provided with some "grants", to showtheir generosity and mercy. Nevertheless, with regard to invest-ment, not a chance.Therefore, foreign investment in China takes the followingshape:by regions, the realized foreign investment in the 18 prov-inces and autonomous regions of the Mid-West accounts for lessthan 10% of the national total even though the area is in greatneed of funds; by industrial sectors, agriculture has only a tinyshare of less than 2% of total foreign investment in China eventhough it cniovs first Prioritv in economic development and de-mands immediate strengthening; likewise, foreign investment 展开更多
关键词 STARTING FROM THE RIGHTS AND WRONGS BY FOREIGN INVESTMENT BETWEEN HONOR AND humiliation
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BETWEEN HONOR AND HUMILIATION: STARTING FROM THE RIGHTS AND WRONGS BY FOREIGN INVESTMENT (Ⅱ)
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作者 Ma Yu 《大经贸》 北大核心 1997年第5期82-86,共5页
Money Scatters Everywhere in China It is said that the saving ratio of China is close to 40%.Authoritative persons thus analyze in this way: theoretically, 40% saving ratio of a country is enough tosustain its economi... Money Scatters Everywhere in China It is said that the saving ratio of China is close to 40%.Authoritative persons thus analyze in this way: theoretically, 40% saving ratio of a country is enough tosustain its economic growth at the rate over 10% even at thetime of its economic take-off; practically, "Four Tigers" inAsia achieved high economic growth in 1960s and 1970s withtheir saving ratio only about 30%. Conclusion: with the 40% saving ratio, or domesticfunds, China can fully sustain the growth rate of 8-9 % thisyear. The question remains: why do we want to introduceforeign funds? Why do we allow foreigners to come and maketens percent of profit while we can only get bits of 展开更多
关键词 BETWEEN HONOR AND humiliation STARTING FROM THE RIGHTS AND WRONGS BY FOREIGN INVESTMENT
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Eliminating a Century-oldNational Humiliation
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《China Today》 1997年第7期7-7,共1页
关键词 In Eliminating a Century-oldNational humiliation
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On Human Dignity as Social Status:A Bottom-Up Approach
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作者 CHEN Xuhui QIAN Chuijun(Translated) 《The Journal of Human Rights》 2025年第1期80-102,共23页
Human dignity is a core concept in contemporary moral philosophy,political science,and jurisprudence.The“social status theory”proposed by Jeremy Waldron argues that human dignity is a social construct,focusing on th... Human dignity is a core concept in contemporary moral philosophy,political science,and jurisprudence.The“social status theory”proposed by Jeremy Waldron argues that human dignity is a social construct,focusing on the fundamental equal social status of individuals.It addresses behaviors of humiliation or devaluation by others,emphasizing both the negative prohibition of such harms and the positive promotion of protections against them.Unlike traditional theories of dignity that often rely on abstract or metaphysical justifications,the social status theory adopts a bottom-up approach grounded in the social practices of dignity.This perspective avoids entanglement in endless value debates over the concept and provides new rationale for affirming human dignity.Furthermore,it refines the understanding of human dignity by identifying three specific types of social harm to dignity and corresponding protective measures.The social status theory significantly mitigates cultural skepticism regarding the concept of human dignity,offering a relatively objective and practicable framework for defining dignity norms.Practically,the theory aligns well with the application of human dignity in both public and private law domains and effectively explains the concept as utilized in international human rights conventions and practices.While there is room for further refinement,the social status theory currently stands out as the most advantageous and robust framework for understanding and promoting human dignity. 展开更多
关键词 human dignity social status RESPECT humiliation
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Selection of Suitable Area and Analysis of Adaptability Difference of Prunus humilis 被引量:1
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作者 Ze YAO Shengxiu JIANG +2 位作者 Zizhu YAN Qi WANG Dawei WANG 《Asian Agricultural Research》 2024年第3期41-45,共5页
This paper delves into and assesses the differences in biological traits and fruit nutritional composition of 3 varieties of Prunus humilis introduced from 2 different regions.Results showed that(i)the contents of pro... This paper delves into and assesses the differences in biological traits and fruit nutritional composition of 3 varieties of Prunus humilis introduced from 2 different regions.Results showed that(i)the contents of protein,total sugar,and calcium of No.5 variety were higher those of the other 2 varieties,which was recognized as the most suitable variety for fresh fruit;(ii)the comparison of leaf areas index and fruit shape index of 3 varieties shows that the fruit shape index and individual fruit weight of the introduced varieties were higher than those of the control,the diameter of individual fruit of the introduced varieties higher than that of the control indicated that they had a higher fruit hardness,more dry matter accumulation and better fruit quality,and that the introduction region was more suitable for scale-up of P.humilis;(iii)there was a positive correlation with the number of fruiting branches and there was a significant negative correlation with individual fruit weight,while the leaf area had a significant negative correlation with the number of fruiting branches.The introduced varieties can develop normally in the 2 testing areas,notably,No.5 variety performed better than No.4 and No.6.Improving management is the prerequisite for maintaining proper number of fruiting branches and high yield. 展开更多
关键词 PRUNUS humilis Biological TRAITS NUTRITIONAL components Introduction and CULTIVATION
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ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acidinduced anthocyanin biosynthesis in Cerasus humilis
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作者 Xiaolong Ji Zhe Li +2 位作者 Mingyu Zhang Shaoyu Lang Xingshun Song 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期51-65,共15页
Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit ... Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration.However,the regulatory network of C.humilis in anthocyanin biosynthesis is still unclear.In this study,the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species.Yeast one-hybrid,dual-luciferase assays,and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C.humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS(MYB-binding elements).ChMYB1 interacted with ChbHLH42and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT.In addition,abscisic acid(ABA)treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C.humilis fruit.Interestingly,ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42.Furthermore,ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42.Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C.humilis fruit,deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops. 展开更多
关键词 Cerasus humilis ANTHOCYANIN ChMYB1 FRUIT ABA
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不同影响因子对干旱半干旱区欧李嫩枝扦插育苗生根的影响
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作者 叶丙鑫 陈小丽 +2 位作者 谢淑琴 王剑 李春花 《林业科技通讯》 2024年第11期76-79,共4页
以欧李(Cerasus humilis)品种‘山农5号’为试材,以当年新生的半木质化新梢为扦插对象,研究不同种类生根剂和浓度、不同留叶数和生根剂蘸根时间对欧李嫩枝扦插育苗生根的影响。不同种类、不同浓度生根剂蘸根试验结果显示,使用200 mg/L A... 以欧李(Cerasus humilis)品种‘山农5号’为试材,以当年新生的半木质化新梢为扦插对象,研究不同种类生根剂和浓度、不同留叶数和生根剂蘸根时间对欧李嫩枝扦插育苗生根的影响。不同种类、不同浓度生根剂蘸根试验结果显示,使用200 mg/L ABT1生根剂,欧李插穗生根效果较好,单株根长为6.53 cm,单株根数为24.4根,生根率为51.9%,成活率为49.5%。不同留叶数和不同生根剂蘸根时间试验结果显示,用200 mg/L ABT1生根剂浸泡2 h,留叶1~3片时,单株根长为6.02~6.10 cm,单株根数为18.5~23.5根;相同的浸根时间下,不同留叶数对欧李插穗的生根率和成活率无显著性影响。综合分析,采用200 mg/L ABT1生根剂蘸根2 h,留叶1~3片时欧李插穗生根效果较好。 展开更多
关键词 欧李 Cerasus humilis 影响因子 嫩枝扦插
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Three Phases of a Person's Ruin:After watching the Movie Rice
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作者 严琦 冷小利 《海外英语》 2014年第19期191-192,共2页
Rice is regarded as a grey film because of its theme of human's evil nature. The dark, secret, and evil sides of human are vividly displayed, which arouse deep thoughts. How is the protagonist, Wulong, ruined? Wha... Rice is regarded as a grey film because of its theme of human's evil nature. The dark, secret, and evil sides of human are vividly displayed, which arouse deep thoughts. How is the protagonist, Wulong, ruined? What to blame for his downfall? In this essay, the writer would try to analyze the phases of his spiritual downfall and discuss the elements which influence the change of his character through a psychological analysis. 展开更多
关键词 the split of PERSONALITY humiliation REVENGE
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Comparison of Photosynthetic Adaptability Between Kobresia humilis and Polygonum viviparum on Qinghai Plateau 被引量:16
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作者 魏捷 余辉 +2 位作者 钟泽璞 匡廷云 贲桂英 《Acta Botanica Sinica》 CSCD 2001年第5期486-489,共4页
The chlorophyll fluorescence parameters of Kobresia humilis Serg. and Polygonum viviparum L. grown at two different altitudes (3?200 m, 3?980 m) were measured and the ultrastructure of chloroplasts were observ... The chlorophyll fluorescence parameters of Kobresia humilis Serg. and Polygonum viviparum L. grown at two different altitudes (3?200 m, 3?980 m) were measured and the ultrastructure of chloroplasts were observed for studying the photosynthetic adaptability of plants to the influences of stress conditions in alpine environment. Rfd _values, the vitality index, in leaves of K. humilis and P.viviparum grown at 3?980 m were higher than those at 3?200 m. The higher ratio of F v/F o and F v/F m in leaves of K. humilis and P.viviparum indicated that the rate of photosynthetic conversion of light energy increased at higher altitude. Ratios of F v/F o and F v/F m and Rfd _values in K.humilis were higher than that in P.viviparum grown at the same altitude. There were more irregular chloroplasts in leaves of both species grown at higher altitude. Many irregular chloroplasts such as swollen thylakoid, deformed chloroplast envelope, were observed in P.viviparum grown at 3?980 m, but few in K. humilis . These results were discussed in relation to the photosynthetic adaptability of alpine plants and the different adaptive competence between K.humilis and P.viviparum . 展开更多
关键词 alpine plants Kobresia humilis Polygonum viviparum chlorophyll fluorescence ultrastructure of chloroplast
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欧李种仁中苦杏仁苷的提取及其抗氧化活性 被引量:8
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作者 邬晓勇 孙雁霞 +2 位作者 何钢 赵琦 苟小军 《湖北农业科学》 北大核心 2013年第19期4764-4767,共4页
采用HPLC法测定欧李(Prunus humilis Bunge)种仁浓缩液中苦杏仁苷的含量,利用DPPH法测定苦杏仁苷的抗氧化性。结果表明,提取液中苦杏仁苷的浓度为7.94 mg/mL;苦杏仁苷清除DPPH的能力随着其浓度的降低而下降,当苦杏仁苷溶液浓度为7.50 mg... 采用HPLC法测定欧李(Prunus humilis Bunge)种仁浓缩液中苦杏仁苷的含量,利用DPPH法测定苦杏仁苷的抗氧化性。结果表明,提取液中苦杏仁苷的浓度为7.94 mg/mL;苦杏仁苷清除DPPH的能力随着其浓度的降低而下降,当苦杏仁苷溶液浓度为7.50 mg/mL时清除率最高,为90.9%。欧李种仁浓缩液经氯仿、乙酸乙酯和加热处理均能提高水相中苦杏仁苷的纯度。 展开更多
关键词 欧李(Prunus humilis Bunge) 种仁 苦杏仁苷 高效液相色谱 抗氧化性
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不同海拔高度矮嵩草的光合响应差异 被引量:11
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作者 韦玉 李熙萌 +2 位作者 桑卫国 周芸芸 冯金朝 《生态科学》 CSCD 北大核心 2014年第6期1160-1164,共5页
高海拔地区具有强光辐射、低CO2分压和低温等环境特征。长期生长在高海拔地区的植物各自形成了其独特的适应特性。为了揭示矮嵩草(Kobresia humilis)对不同海拔的光合生理适应机制,使用Li-6400便携式光合作用测量系统观测了大阪山阴坡... 高海拔地区具有强光辐射、低CO2分压和低温等环境特征。长期生长在高海拔地区的植物各自形成了其独特的适应特性。为了揭示矮嵩草(Kobresia humilis)对不同海拔的光合生理适应机制,使用Li-6400便携式光合作用测量系统观测了大阪山阴坡生长于3个不同海拔高度(3000 m、3400 m、3800 m)矮嵩草光响应和CO2响应曲线。结果显示,在测量温度下,矮嵩草的最大净光合速率和光合能力随海拔梯度升高而逐渐提高;高海拔地区生长的矮嵩草具有较高的羧化效率,而低海拔地区生长的矮嵩草具有较高的表观量子效率。高海拔地区生长的矮嵩草具较高的光饱和点,较低的CO2补偿点和饱和点,而不同海拔间的光呼吸速率无显著差异。研究结果表明,矮嵩草表现出对高海拔地区环境积极的适应方式。 展开更多
关键词 矮嵩草(Kobresia humilis) 海拔梯度 光响应曲线 CO2响应曲线
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鹿角珊瑚人工养殖的初步研究 被引量:9
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作者 鲍鹰 周学家 +5 位作者 黄美霞 梁凯珊 黄雪芬 黄少秋 邱庆昌 郑小欧 《海洋科学》 CAS CSCD 北大核心 2012年第1期69-72,共4页
为了探讨珊瑚人工养殖的基本条件和方法,分别在陆基水泥池中和海底珊瑚苗床上对3种鹿角珊瑚:粗野鹿角珊瑚(Acropora humilis)、霜鹿角珊瑚(Acropora pruinosa)和松枝鹿角珊瑚(Acropora brueggemanni)的人工养殖进行了实验。结果表明,这... 为了探讨珊瑚人工养殖的基本条件和方法,分别在陆基水泥池中和海底珊瑚苗床上对3种鹿角珊瑚:粗野鹿角珊瑚(Acropora humilis)、霜鹿角珊瑚(Acropora pruinosa)和松枝鹿角珊瑚(Acropora brueggemanni)的人工养殖进行了实验。结果表明,这3种珊瑚可以在深圳市鹅公湾近岸海域的海底珊瑚苗床上常年生长;在水泥池中当水温低于14℃或高于28℃时珊瑚陆续死亡;这3种珊瑚的生长速度在适温范围内随着水温的升高而加快,最大月生长值是6.05 mm;光照对这3种珊瑚的生长有影响,直接的阳光照射会引起水泥池中珊瑚的死亡。 展开更多
关键词 鹿角珊瑚(Acropora humilis) 养殖
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欧李浑浊果汁加工工艺的研究 被引量:2
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作者 王芹 蔡金星 +2 位作者 刘秀凤 庞杰 张长峰 《山东农业科学》 2012年第6期110-115,共6页
以欧李为原料,采用单因素试验确定欧李果浆加水量、适合稳定剂,采用正交试验筛选酶解最佳条件和最佳配方。随着加水量的增加,欧李果浆粘稠度降低,欧李原有香味变淡;适当酶解条件下欧李原果汁的出汁率显著提高;糖浓度为10%、原果汁浓度65... 以欧李为原料,采用单因素试验确定欧李果浆加水量、适合稳定剂,采用正交试验筛选酶解最佳条件和最佳配方。随着加水量的增加,欧李果浆粘稠度降低,欧李原有香味变淡;适当酶解条件下欧李原果汁的出汁率显著提高;糖浓度为10%、原果汁浓度65%、柠檬酸浓度0.12%~0.16%的果汁口味、颜色较佳;黄原胶单独使用时的浑浊果汁稳定性良好;制得成品营养丰富。制备欧李果浆的加水量(质量比):欧李∶水=1∶2;最佳酶解条件:果胶酶浓度0.05%,酶解温度30℃,酶解时间1.5 h;最佳配方(质量分数):原汁浓度65%,糖浓度10%,柠檬酸浓度0.12%;稳定剂选择黄原胶,浓度0.1%。本研究为欧李浑浊果汁加工与存放中的稳定性提供技术保障,拓宽了欧李果实的加工利用途径。 展开更多
关键词 欧李(PmnW humilis) 浑浊果汁 酶解 稳定性
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Study on the Influencing Factors and the Root Mechanism in Cutting Process of Prunus humilis(Bge). Sok
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作者 杨秀峰 李凤兰 +2 位作者 孙旭红 佟伟霜 胡宝忠 《Agricultural Science & Technology》 CAS 2009年第2期113-117,共5页
A procedure for cutting Prunus humilis(Bge). Sok was comprehensively studied in this paper. It was found that the key factors involved in this procession were medium, rooting accelerator, concentration of rooting ac... A procedure for cutting Prunus humilis(Bge). Sok was comprehensively studied in this paper. It was found that the key factors involved in this procession were medium, rooting accelerator, concentration of rooting accelerator and type of shoot. The results showed that send was used as mediums; Treatment with 1 000 mg/L AST rooting powder No. 2 and semi-woody shoots were the optimal materials for cutting, and the rooting rate reached 88.1%. Anatomical study on rooting of Prunus hum#is(Bge). Sok cutting has been carded out by the paraffin section method. The observation result shows that the adventitious root primordium of Prunus humili$(Bge). Sok cutting belongs to the type of induced root primordium. The adventitiousroot primordium originates from the cross region of vascular cambium and pith rays. 展开更多
关键词 Prunus humilis(Bge). Sok Green shoot cutting Adventitious roots ANATOMY
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欧李嫁接技术 被引量:2
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作者 张永信 安传志 李辉 《河北果树》 2016年第4期46-47,共2页
欧李(Gerasus humilis(Bge.)Sok)是蔷薇科樱属的一种落叶小灌木,因其果实含钙量居水果之首也称"钙果",是我国北方特有的第3代水果典型树种。欧李树体矮小,株高1.2-1.5 m,丛生。根系发达,根冠比为9∶1。4月开白花或粉红花,形似樱花... 欧李(Gerasus humilis(Bge.)Sok)是蔷薇科樱属的一种落叶小灌木,因其果实含钙量居水果之首也称"钙果",是我国北方特有的第3代水果典型树种。欧李树体矮小,株高1.2-1.5 m,丛生。根系发达,根冠比为9∶1。4月开白花或粉红花,形似樱花,极具观赏性,果似樱桃,味似李子,酸甜爽口,风味独特。单果质量5-15 g,果肉厚。果实有红、黄、紫等多种颜色。根系由主根、侧根组成,垂直分布达1-2 m,侧根伸向四周1-1.5 m,为深根性植物。 展开更多
关键词 欧李 嫁接技术 humilis 钙果 含钙量 樱属 单果质量 深根性 根冠比 垂直分布
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浙江发现解氏狭臀蚤
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作者 卢苗贵 赵芝雅 +4 位作者 叶小娜 叶仙萍 胡萍 吴军民 陈用琴 《浙江预防医学》 1991年第1期41-41,共1页
最近,在鉴定从浙江省义乌市采到的蚤类标本中发现狭臀蚤属(Genus Stenischia Jordan,1932)解氏狭臀蚤(S.Xiei Li.1987)一雌蚤,为本省首次纪录。现予报道。鉴别特征本蚤与奇异狭臀蚤(S.mirabilis Jordan,1932)、金氏狭臀蚤(S.chini Xie e... 最近,在鉴定从浙江省义乌市采到的蚤类标本中发现狭臀蚤属(Genus Stenischia Jordan,1932)解氏狭臀蚤(S.Xiei Li.1987)一雌蚤,为本省首次纪录。现予报道。鉴别特征本蚤与奇异狭臀蚤(S.mirabilis Jordan,1932)、金氏狭臀蚤(S.chini Xie et Lin,1989)和低地狭臀蚤(S.humilis Xie 展开更多
关键词 低地狭臀蚤 humilis GENUS 鉴别特征 蚤属 金氏 蚤类 JORDAN 颊栉 浙江省义乌市
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Adichie's Purple Hibiscus and the Issue of Feminism in African Novel
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作者 Ibeku Ijeoma Ann 《Journal of Literature and Art Studies》 2015年第6期426-437,共12页
Adichie's Purple Hibiscus is a feminist work that challenges the dehumanizing tendencies of the menfolk as evident in the character of Mama (Beatrice Achike) who eventually exposed the African conception of an idea... Adichie's Purple Hibiscus is a feminist work that challenges the dehumanizing tendencies of the menfolk as evident in the character of Mama (Beatrice Achike) who eventually exposed the African conception of an ideal woman who keeps dumb even in the face of humiliation, victimization, and brutality so as to be perceived as a good woman. We will make a resounding case to portray that Achike belongs to the category of liberal feminism. However, as events unfolds, she was forced by situations beyond her control to respond and go radical in order to crush anything that stands in her way to happiness. Efforts will be made to show how African women are rated based on the real and good women as represented by Ifeoma and Beatrice Achike respectively. This essay in exploring the different tenets of feminism will acknowledge that radical feminism is an off shoot of violence. We shall argue that radical feminism is a radical reaction to dehumanization, humiliation, and violence. This study seeks to address the issue of feminism and how characters subject to series of humiliation that leads to a radical approach to gain their freedom. 展开更多
关键词 FEMINISM humiliation subjugation CATHOLIC fanatic PATRIARCHY real woman good woman Africa
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从嵩草属植物看生物多样性
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作者 杨勇刚 杨福囤 《青海环境》 1994年第4期170-173,共4页
保护生物多样性日益引起人们的重视,也是当前国际上重大的环境问题之一。由于生物多样性是人类赖以生存的条件和经济发展的物质基础,故1992年6月在巴西召开了联合国环境与发展大会,大多数国家元首或政府首脑在《生物多样性公约》上签字... 保护生物多样性日益引起人们的重视,也是当前国际上重大的环境问题之一。由于生物多样性是人类赖以生存的条件和经济发展的物质基础,故1992年6月在巴西召开了联合国环境与发展大会,大多数国家元首或政府首脑在《生物多样性公约》上签字。提高环境保护意识已刻不容缓。1994年6月12日我国又召开专门会议,并正式开始实施《中国生物多样性保护行动计划》。 展开更多
关键词 嵩草 生物多样性 环境问题 高山唐松草 黑褐苔草 政府首脑 杂类草 矮火绒草 异针茅 humilis
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欧李ChIRT1基因的克隆与生物信息学分析 被引量:1
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作者 林琳 张雪萌 +1 位作者 郝梦琪 郭东林 《分子植物育种》 CAS 北大核心 2021年第3期808-814,共7页
欧李(Cerasus humilis)是蔷薇科矮生灌木,果实中的钙、铁等营养物质丰富。铁调转运基因1(IRT1)编码的蛋白是ZIP金属转运家族中主要的铁转运蛋白,负责植物中金属离子的转运,尤其是铁的吸收。本研究从欧李叶片中提取RNA,逆转录得到cDNA;... 欧李(Cerasus humilis)是蔷薇科矮生灌木,果实中的钙、铁等营养物质丰富。铁调转运基因1(IRT1)编码的蛋白是ZIP金属转运家族中主要的铁转运蛋白,负责植物中金属离子的转运,尤其是铁的吸收。本研究从欧李叶片中提取RNA,逆转录得到cDNA;参照小金海棠(Malus xiaojinensis)MxIRT1基因设计引物;克隆了欧李ChIRT1基因,并进行了生物信息学分析。结果表明,ChIRT1全长1417 bp,含有1个678 bp的开放阅读框,编码226个氨基酸。推测ChIRT1蛋白的分子量约为24 kD,理论等电点为7.72,含有4个跨膜结构域,属于跨膜蛋白。ChIRT1含有一个ZIP结构域,二级结构中α-螺旋占44.69%,无规则卷曲占38.94%。系统发育分析表明,欧李的ChIRT1基因与蔷薇科的IRT1基因关系密切,与樱桃李(Prunus persica)的PpIRT1基因相似度最高。本研究克隆了欧李ChIRT1基因,并分析了其序列特征,为研究欧李ChIRT1基因功能及铁调节机制提供了依据。 展开更多
关键词 欧李(Cerasus humilis) IRT基因 基因克隆 生物信息学分析
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欧李ChUFGT基因的克隆、生物信息学分析及亚细胞定位 被引量:2
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作者 王迪 成冠超 +3 位作者 祝利伟 刘宇涵 笈小龙 宋兴舜 《分子植物育种》 CAS 北大核心 2023年第3期782-788,共7页
为从分子水平上揭示欧李中3-O-类黄酮葡萄糖基转移酶(UFGT)基因的空间表达情况,以欧李叶片为试材,根据欧李的转录组序列设计特异性引物,通过PCR获得ChUFGT基因序列,并进行生物信息学分析及亚细胞定位分析。结果表明,ChUFGT基因DNA开放... 为从分子水平上揭示欧李中3-O-类黄酮葡萄糖基转移酶(UFGT)基因的空间表达情况,以欧李叶片为试材,根据欧李的转录组序列设计特异性引物,通过PCR获得ChUFGT基因序列,并进行生物信息学分析及亚细胞定位分析。结果表明,ChUFGT基因DNA开放阅读框为1425 bp(不含终止子),相对分子质量为51.63 kD,编码475个氨基酸,其对应的蛋白质是带正电荷的稳定疏水性蛋白。与近缘物种的蛋白质序列比对分析显示,欧李、李和樱桃李的UFGT氨基酸序列相似度达到97.22%和96.62%,整个系统发育树存在两个大分支,其中ChUFGT与同科植物的亲缘关系最近,其次是与白梨、西洋梨、荔枝等其他科植物相对较近,与葡萄、刺葡萄、石榴等植物的亲缘关系最远。ChUFGT的亚细胞定位结果分析显示,在洋葱内表皮细胞的细胞核和细胞质中都表达该基因。本研究结果为进一步阐明ChUFGT调控欧李细胞花色素苷的合成机制提供了理论基础。 展开更多
关键词 欧李(Cerasus humilis) UFGT基因 基因克隆 生物信息学分析 亚细胞定位
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